LOCUS       sequence-420510-        8548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express tagged cyclin D1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Konagaya Y, Rosenthal D, Ratnayeke N, Fan Y, Meyer T
  TITLE     An intermediate Rb-E2F activity state safeguards proliferation 
            commitment.
  JOURNAL   Nature. 2024 Jul;631(8020):424-431. doi: 10.1038/s41586-024-07554-2.
            Epub 2024 Jun 26.
  PUBMED    38926571
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07554-2"; journalName: "Nature"; date: 
            "2024-07"; volume: "631"; issue: "8020"; pages: "424-431"
COMMENT     Sequence Label: pCW-CCND1-HA-puro
FEATURES             Location/Qualifiers
     source          1..8548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1150..1159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1449..1475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1490..2000
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1919..1937
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2010..2606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2010..2029)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2506..2526
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2607..2660
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2661..3278,3279..3284,3285..3320,3321..3323,3324..3359,
                     3360..3362,3363..3398,3399..3407)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3447..3467)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3865..3876)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4081..4314
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4386..4507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4423..4442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4477..4496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4547..4682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4682..4753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4754..4822,4823..5614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4972..4991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5785..6373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6274..6293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6527..6544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6661..6682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6697..6702,6703..6720,6721..6727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6735..6751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6740..6762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6759..6775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6759..6775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6794..6814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6796..6814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6842..7068
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7069..7249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7296..7421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7914..8147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8332..8376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggaacaccag ctcctgtgct gcgaagtgga aaccatccgc cgcgcgtacc
      601 ccgatgccaa cctcctcaac gaccgggtgc tgcgggccat gctgaaggcg gaggagacct
      661 gcgcgccctc ggtgtcctac ttcaaatgtg tgcagaagga ggtcctgccg tccatgcgga
      721 agatcgtcgc cacctggatg ctggaggtct gcgaggaaca gaagtgcgag gaggaggtct
      781 tcccgctggc catgaactac ctggaccgct tcctgtcgct ggagcccgtg aaaaagagcc
      841 gcctgcagct gctgggggcc acttgcatgt tcgtggcctc taagatgaag gagaccatcc
      901 ccctgacggc cgagaagctg tgcatctaca ccgacaactc catccggccc gaggagctgc
      961 tgcaaatgga gctgctcctg gtgaacaagc tcaagtggaa cctggccgca atgaccccgc
     1021 acgatttcat tgaacacttc ctctccaaaa tgccagaggc ggaggagaac aaacagatca
     1081 tccgcaaaca cgcgcagacc ttcgttgccc tctgtgccac agatgtgaag ttcatttcca
     1141 atccgccctc catggtggca gcggggagcg tggtggccgc agtgcaaggc ctgaacctga
     1201 ggagccccaa caacttcctg tcctactacc gcctcacacg cttcctctcc agagtgatca
     1261 agtgtgaccc ggactgcctc cgggcctgcc aggagcagat cgaagccctg ctggagtcaa
     1321 gcctgcgcca ggcccagcag aacatggacc ccaaggccgc cgaggaggag gaagaggagg
     1381 aggaggaggt ggacctggct tgcacaccca ccgacgtgcg ggacgtggac atcagcgctg
     1441 ggggatccta tccgtacgac gtaccagact acgcatgaga attcgatccg gggttggggt
     1501 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     1561 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     1621 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     1681 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     1741 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     1801 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     1861 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     1921 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     1981 tcaccgacct ctctccccag caattcacca tgaccgagta caagcccacg gtgcgcctcg
     2041 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     2101 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     2161 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     2221 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     2281 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     2341 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     2401 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     2461 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     2521 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     2581 ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc tctcctcact tgtggtgatg
     2641 ttgaagaaaa ccctggtcca atgtctagac tggacaagag caaagtcata aacggagctc
     2701 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     2761 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     2821 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     2881 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     2941 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     3001 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     3061 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     3121 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     3181 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     3241 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     3301 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     3361 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc
     3421 gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3481 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3541 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3601 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3661 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3781 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     3841 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3901 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3961 tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag gtacctttaa
     4021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4081 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4321 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4381 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4501 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4681 cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6661 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6781 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     6841 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     6901 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     6961 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     7021 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     7081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7261 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     7321 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     7381 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     7441 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     7501 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     7561 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     7621 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     7681 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     7741 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     7801 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     7861 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     7921 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     7981 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8041 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8101 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8161 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8221 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     8281 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     8341 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     8401 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     8461 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     8521 cagacccacc tcccaacccc gaggggac
//
