LOCUS       sequence-420516-        9831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Venus under CDC6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Konagaya Y, Rosenthal D, Ratnayeke N, Fan Y, Meyer T
  TITLE     An intermediate Rb-E2F activity state safeguards proliferation 
            commitment.
  JOURNAL   Nature. 2024 Jul;631(8020):424-431. doi: 10.1038/s41586-024-07554-2.
            Epub 2024 Jun 26.
  PUBMED    38926571
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07554-2"; journalName: "Nature"; date: 
            "2024-07"; volume: "631"; issue: "8020"; pages: "424-431"
COMMENT     Sequence Label: pLV-hCDC6p-Venus-puro
FEATURES             Location/Qualifiers
     source          1..9831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     regulatory      3508..3517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3514..3516,3517..3519,3520..4230)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3559..3580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3820..3839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4167..4188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4243..4263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4426..4650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4657..5167
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5086..5104
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5177..5776
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5177..5196)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5673..5693
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5800..6388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5853..5873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6271..6282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6595..7228
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7356..7372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7356..7372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7369..7391)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7380..7396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7404..7410,7411..7428,7429..7434))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7449..7470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7587..7604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7758..8346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7838..7857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8517..9308,9309..9377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9140..9159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9378..9482)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9530..9664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9567..9586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9621..9640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataccggt caattcactg atgatcgtaa tattgttgct
     2221 tccctctttc taggctttat ggctctattt tgtttgttac tgaggggtaa aagataaatg
     2281 tttaccatca cctagaattg ggttctggcc tctaaaggaa cctgaggctt agatgaatta
     2341 ttggctttgg aagctggcct tcaaattact gcgctaattt atatttttca ttaaaaactc
     2401 agcttgcctc ttctatatag ctgtcttccc tggccctgaa accctagtgt ttcgccataa
     2461 aagattttaa aattaagggg tcataattcc ctccccatga tgtgtggatt aatggtaaga
     2521 agatgcacag aacataatat tcttaggttg aacgaaataa aagtaaagag ttggctctgt
     2581 ttctcacctt tgaagcacaa atcaagagat actatgatga agcatagttt ttctttatat
     2641 aggtgtgtag aactttacca taaaaatcac tagttcagcc atcaggagat ctggatccta
     2701 ggctcttcac tgtcaccaag atgctgtgac ctctaacctt gtatagaagt ttgctttgta
     2761 ctttgcgagg ttgagcatta gagaggtaag gaaagtgcct agcatcatac ctggcgcaca
     2821 gaacccaaaa cggtaggtat catgtagcag ttctgaaaat ctagcccatc aggatgatgc
     2881 aaatgggtac tttaggcagt gagaagggga accacatctt gacacttcca gtcgaaggaa
     2941 gagtgcgact gcgcggcagc aaagactacg cctcccagcg tgctttgcgg cgggccggcc
     3001 cgctttaccc agagtcgccc tgccgcaatc gcgcgtcttt ccaccgaggc cccggatgta
     3061 gattccctcc cccgttcagt ggtcgtggcc tcacagcgac tctaagactt ggggctctct
     3121 cattggctgt aactcttcca ctggattggt agcaaaaaaa gaggcggtgc ccaaggcgaa
     3181 aggctctgtg actacagcca atcagaatcg aggccgggct ttggcgggag gtgggaacgc
     3241 tgtggccatt cggatttggc gcgagcgcgg ctggagtttg ctgctgccgc tgtgcagttt
     3301 gttcaggggc ttgtggtggt gagtccgaga ggctgcgtgt gagagacgtg agaaggatcc
     3361 tgcactgagg aggtggaaag aagaggattg ctcgaggagg cctggggtct gtgaggcagc
     3421 ggagctgggt gaaggctgcg ggttccggcg aggcctgagg tgaagtgaag ggaaaagagc
     3481 tgagctttcg aaactagtaa ttcgagcgcc accatggtga gcaagggcga ggagctgttc
     3541 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3601 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     3661 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     3721 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3781 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3841 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3901 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3961 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     4021 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     4081 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     4141 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4201 atcactctcg gcatggacga gctgtacaag ggaagtggtg gaccaaagaa gaagagaaag
     4261 gtaggtagtg gacatggctt cccgccggca gtggcagcac aggatgatgg cacgctgccc
     4321 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     4381 atcaatgtgt aggaattctc tagacctagg ggatcctgat cacgactgtg ccttctagtt
     4441 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4501 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4561 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4621 ggcatgctgg ggatgcggtg ggctctatgg ctcgaggggg ttggggttgc gccttttcca
     4681 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     4741 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     4801 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     4861 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     4921 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     4981 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     5041 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     5101 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     5161 tccccagcaa ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5221 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5281 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5341 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5401 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5461 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5521 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5581 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5641 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5701 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5761 caagcccggt gcctgacccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa
     5821 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5881 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5941 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6001 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6061 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6121 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6181 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6241 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6301 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6361 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6421 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6481 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6541 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     6601 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6661 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     6721 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     6781 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6841 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     6901 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     6961 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7021 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7201 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7261 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
     7321 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7381 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7441 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7501 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7561 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7621 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7681 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8161 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8341 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8401 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8461 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8521 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8581 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8641 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8701 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8761 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8821 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8881 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8941 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9001 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9061 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9121 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9181 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9241 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9301 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9361 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9421 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9481 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
     9541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9661 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9721 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9781 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
