LOCUS       sequence-420523-        6669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human C17orf53 N-terminally fused to mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6669)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmVen-C17orf53
FEATURES             Location/Qualifiers
     source          1..6669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        94..397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..601
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     552..572
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      640..649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(646..648,649..651,652..1362)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(691..712)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(952..971)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1299..1320
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3490..3611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3527..3546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3581..3600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3618..4073)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3755..3776)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3967..3986
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4100..4204
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4206..4563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4206..4397
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4229..4249)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4414..4549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4598..5392
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4598..5392
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4652..4671)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5262..5281
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5580..5599)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5624..5671
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6000..6588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6489..6508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
       61 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      601 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
      661 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      721 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      781 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      841 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      901 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      961 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1021 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1081 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1141 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     1201 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1261 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1321 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga
     1381 gctcaagctt cgaattctat ggcgtgcagt ttgcagaagc tgtttgctgt ggaagaggag
     1441 tttgaagatg aggatttctt gtctgctgtg gaggatgcag agaaccggtt tactggctca
     1501 ctgcctgtga atgctgggcg cctgagacct gtctcttcta ggccacagga gactgtgcag
     1561 gcacagtcct ccaggctgct gctgttacac cccactgctc cctcagaggc tttgggcctg
     1621 ccagacttgg acctctgcct ccctgcctcc agcacgccca gtgctgacag ccgtccatca
     1681 tgcataggag cagctcccct aaggcctgtc tctacttcca gcagctggat tggcaatcag
     1741 agaagagtga cagtgacaga agtgctcaga gagacagcaa gacctcagtc ctcagcctta
     1801 caccccctac tcacctttga gagccaacag cagcaagttg gtggctttga ggggcctgaa
     1861 caagacgaat ttgataaagt cctggcaagc atggagttgg aggagcctgg catggagctg
     1921 gaatgtggag tcagcagtga ggccatacca atcctgcctg cccagcagcg ggagggttca
     1981 gtattggcta aaaaagcccg ggtagttgat ctgagtggat cttgccagaa ggggcctgtg
     2041 cctgccatcc acaaagcggg tatcatgtcc gcccaggatg agtctctaga tcctgtcatc
     2101 caatgtagga ctccacgacc ccccttgaga cctggtgctg tgggtcacct tcctgttcca
     2161 actgccttaa cagttcccac tcagcaactc cactgggaag tctgtccgca acgctcccct
     2221 gttcaagcac ttcagcctct ccaagctgct agagggacca ttcagagcag ccctcaaaat
     2281 cgtttccctt gtcagccatt ccagtctcca agttcctggt taagtggcaa agctcattta
     2341 cccagacctc gaactcccaa ctcaagctgt tctactccct caaggactag ctctggatta
     2401 tttcctcgga tacccttaca accgcaagct ccagtgtctt ccattgggtc tcctgttggt
     2461 accccaaaag gtccccaggg agctctgcag acacccatag tcaccaacca cctggtgcag
     2521 ctagtcactg ctgccagccg gacaccccag cagcccaccc atccctccac ccgagccaaa
     2581 actcgccgtt tccctggccc agctgggatc ctgcctcacc agcagagtgg gagaagtctg
     2641 gaggacatca tggtttccgc gccccaaact ccaacccatg gtgctctggc taaattccag
     2701 acagagattg ttgctagttc ccaggcatct gtggaggagg attttgggcg agggccctgg
     2761 ctgaccatga aatccacgct aggcctggat gagagagacc ctagctgctt cctctgtacc
     2821 tacagcattg tcatggtgct gcgcaaggca gccctgaagc agcttcctag gaacaaggtc
     2881 cccaacatgg cggtgatgat caagtccctg actcggagca caatggacgc cagtgtggtt
     2941 ttcaaggacc ccacgggaga gatgcagggg acggtgcaca ggttgctgct ggagacgtgc
     3001 cagaatgagc tgaagcctgg ctcagtgctg ctgctgaagc agattggagt gttttctcct
     3061 tcacttcgaa atcactacct caacgtgaca cccaacaacc tggtccatat ttacagcccg
     3121 gattctgggg atgggagctt cctcaagcca tctcagccct tccccaagga ttcagggagc
     3181 ttccagcatg atgtggctgc aaagcccgag aaaggcttca gaacagcaca gaacctagag
     3241 gcagaggcgt cccctgagga agaactccca gaagcagatg acctggatgg actcctgagt
     3301 gagcttcctg aagacttctt ctgtgggacc agtagttgag tcgacggtac cgcgggcccg
     3361 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     3421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3601 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3721 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3841 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4021 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4201 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4381 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4441 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4501 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4561 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4621 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4681 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4741 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4801 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4861 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4921 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4981 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5041 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5101 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     5161 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5221 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5281 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5341 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5401 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5461 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5521 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5581 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5641 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5701 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5761 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5821 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6661 ttatcccct
//
