LOCUS       sequence-420567-        9744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2
FEATURES             Location/Qualifiers
     source          1..9744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     566..582
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     567..583
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    602..1168
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    618..1170
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(762..779)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     989..1008
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1182..1184,1185..1187,1188..1901)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1227..1248)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1488..1507)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1835..1856
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2004..2518
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2680..2696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2704..2710,2711..2728,2729..2734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2749..2770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2887..2904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3058..3646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3138..3157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3817..4608,4609..4677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4440..4459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4678..4782)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4850..4868
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4906..4928)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5028..5047
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(5078..5125)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5173..5502)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5187..5322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5241..5260)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5487..5507
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     5693..5715
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5843..5862
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6062..6578
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6642..6983
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7050..7466
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7396..7418
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7434..7450
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      9669..9678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggccgtact ctaccagcgg agcaagagcc tgagccccag cctgctgggc tatcagcagc
       61 cggccctcat ggccgccccg ctgtcccttg cggacgctca ccgctctgtg ctggtgcacg
      121 ccggctccca gggccagagc tcagccctgc tccacccctc tccgaccaac cagcaggcct
      181 cgcctgtgat ccactactca cccaccaacc agcagctgcg ctgcggaagc caccaggagt
      241 tccagcacat catgtactgc gagaatttcg caccaggcac caccagacct ggcccgcccc
      301 cggtcagtca aggtcagagg ctgagcccgg gttcctaccc cacagtcatt cagcagcaga
      361 atgccacgag ccaaagagcc gccaaaaacg gacccccggt cagtgaccaa aaggaagtat
      421 tacctgcggg ggtgaccatt aaacaggagc agaacttgga ccagacctac ttggatgatg
      481 ttaatgaaat tatcaggaag gagttttcag gacctcctgc cagaaatcag acgtaagcga
      541 tcgcttataa gcggccgcgt cgaggtcgag gtcgacggta tcgataagct tgatatcgaa
      601 ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
      661 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
      721 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
      781 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
      841 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
      901 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
      961 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     1021 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     1081 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     1141 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     1201 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1261 agttcagcgt gtctggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1321 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1381 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1441 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1501 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1561 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1621 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gcgaacttca
     1681 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1741 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1801 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1861 ccgccgggat cactctcggc atggacgagc tgtacaagta atgaattaat taagaattcc
     1921 tgcagcccgg gggatccgtc gacctgcagc caagcttatc gataaaataa aagattttat
     1981 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     2041 aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc
     2101 aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt
     2161 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
     2221 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     2281 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     2341 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
     2401 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     2461 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg
     2521 gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa
     2581 tcctgactca attagccact gttttgaatc cacatactcc aatactcctg aaatagttca
     2641 ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc tcacaattcc
     2701 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2761 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2821 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2881 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2941 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3481 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3901 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4141 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4441 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4681 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4741 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4801 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4861 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4921 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4981 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5041 gattgtactg agagtgcacc atatggttcc tcaccttgtc gtattatact atgccgatat
     5101 actatgccga tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc
     5161 tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     5221 tagctcagag gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     5281 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     5341 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5401 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5461 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gcatatgcgg
     5521 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     5581 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     5641 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     5701 cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     5761 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     5821 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     5881 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta
     5941 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     6001 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     6061 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     6121 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     6181 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6241 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6301 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6361 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6421 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca
     6481 ataaagcctc ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc
     6541 tcagattgat tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg
     6601 agacccctgc ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt
     6661 tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc
     6721 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     6781 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     6841 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     6901 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     6961 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     7021 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     7081 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     7141 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     7201 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     7261 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     7321 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     7381 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     7441 cagccctcac tccttctcta ggcgccggaa ttagatctgc ggccgcggat ttaaatccca
     7501 ccatgaacgc ccccgagcgg cagccccaac ccgacggcgg ggacgcccca ggccacgagc
     7561 ctgggggcag cccccaagac gagcttgact tctccatcct cttcgactat gagtatttga
     7621 atccgaacga agaagagccg aatgcacata aggtcgccag cccaccctcc ggacccgcat
     7681 accccgatga tgtcctggac tatggcctca agccatacag cccccttgct agtctctctg
     7741 gcgagccccc cggccgattc ggagagccgg atagggtagg gccgcagaag tttctgagcg
     7801 cggccaagcc agcaggggcc tcgggcctga gccctcggat cgagatcact ccgtcccacg
     7861 aactgatcca ggcagtgggg cccctccgca tgagagacgc gggcctcctg gtggagcagc
     7921 cgcccctggc cggggtggcc gccagcccga ggttcaccct gcccgtgccc ggcttcgagg
     7981 gctaccgcga gccgctttgc ttgagccccg ctagcagcgg ctcctctgcc agcttcattt
     8041 ctgacacctt ctccccctac acctcgccct gcgtctcgcc caataacggc gggcccgacg
     8101 acctgtgtcc gcagtttcaa aacatccctg ctcattattc ccccagaacc tcgccaataa
     8161 tgtcacctcg aaccagcctc gccgaggaca gctgcctggg ccgccactcg cccgtgcccc
     8221 gtccggcctc ccgctcctca tcgcctggtg ccaagcggag gcattcgtgc gccgaggcct
     8281 tggttgccct gccgcccgga gcctcacccc agcgctcccg gagcccctcg ccgcagccct
     8341 catctcacgt ggcaccccag gaccacggct ccccggctgg gtacccccct gtggctggct
     8401 ctgccgtgat catggatgcc ctgaacagcc tcgccacgga ctcgccttgt gggatccccc
     8461 ccaagatgtg gaagaccagc cctgacccct cgccggtgtc tgccgcccca tccaaggccg
     8521 gcctgcctcg ccacatctac ccggccgtgg agttcctggg gccctgcgag cagggcgaga
     8581 ggagaaactc ggctccagaa tccatcctgc tggttccgcc cacttggccc aagccgctgg
     8641 tgcctgccat tcccatctgc agcatcccag tgactgcatc cctccctcca cttgagtggc
     8701 cgctgtccag tcagtcaggc tcttacgagc tgcggatcga ggtgcagccc aagccacatc
     8761 accgggccca ctatgagaca gaaggcagcc gaggggctgt caaagctcca actggaggcc
     8821 accctgtggt tcagctccat ggctacatgg aaaacaagcc tctgggactt cagatcttca
     8881 ttgggacagc tgatgagcgg atccttaagc cgcacgcctt ctaccaggtg caccgaatca
     8941 cggggaaaac tgtcaccacc accagctatg agaagatagt gggcaacacc aaagtcctgg
     9001 agatcccctt ggagcccaaa aacaacatga gggcaaccat cgactgtgcg gggatcttga
     9061 agcttagaaa cgccgacatt gagctgcgga aaggcgagac ggacattgga agaaagaaca
     9121 cgcgggtgag actggttttc cgagttcaca tcccagagtc cagtggcaga atcgtctctt
     9181 tacagactgc atctaacccc atcgagtgct cccagcgatc tgctcacgag ctgcccatgg
     9241 ttgaaagaca agacacagac agctgcctgg tctatggcgg ccagcaaatg atcctcacgg
     9301 ggcagaactt tacatccgag tccaaagttg tgtttactga gaagaccaca gatggacagc
     9361 aaatttggga gatggaagcc acggtggata aggacaagag ccagcccaac atgctttttg
     9421 ttgagatccc tgaatatcgg aacaagcata tccgcacacc tgtaaaagtg aacttctacg
     9481 tcatcaatgg gaagagaaaa cgaagtcagc ctcagcactt tacctaccac ccagtcccag
     9541 ccatcaagac ggagcccacg gatgaatatg accccactct gatctgcagc cccacccatg
     9601 gaggcctggg gagccagcct tactaccccc agcacccgat ggtggccgag tccccctcct
     9661 gcctcgtggc caccatggct ccctgccagc agttccgcac ggggctctca tcccctgacg
     9721 cccgctacca gcaacagaac ccag
//
