LOCUS       sequence-420569-       17557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  helper plasmid for bacterial conjugal DNA transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
  TITLE     Reduction of conjugal transfer efficiency by three restriction 
            activities of Anabaena sp. strain PCC 7120.
  JOURNAL   J Bacteriol. 1997 Mar;179(6):1998-2005.
  PUBMED    9068647
REFERENCE   2  (bases 1 to 17557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1997-03"; volume: "179(6)"; pages: "1998-2005"
COMMENT     Sequence Label: pRL623
FEATURES             Location/Qualifiers
     source          1..17557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8890..8909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9130..9147
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9388..9410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     16007..16026
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttaagggtta atattttatt gtaagaagaa ggccactcat aatcgatgcc atcagcaaga
       61 tcatttctta cagctacaat gatcaaacgc tcgcgttttt gcggcacttt gtagaaaata
      121 gccttaagca ctcttggctc aaataaagtg tagcccaagt ctgtgataat attttttatt
      181 gtttctaaag ttcgtccagc atcatgattt agcaaccctc gaacattctc tgctaaaaga
      241 actttcggat tgatttcttt agcggctcgg gcgaattcaa agaaaagggt gccccgtgta
      301 tcttcaaaac caagtttttt gcctgcataa gagaatgcct ggcaaggaaa gccaccagcc
      361 agcacatcaa cggtattcct ataaggggtg aagtctactt ggctcacatc accttcaacc
      421 acattccatt caggcctatt ttttcgtaac gtcttacaag catgggagtc aatttcattt
      481 agtaaaacag attttaaacc ggctttttct aaacctaaag ccatcccccc tgcgccagca
      541 aataactcta atacagtata aacattattg cttattttag tctcatcagg ccactggctt
      601 ttaaataaaa actgagcttg ttcaaaattt ttaagttgct cttttttata aaatcgatag
      661 ttgttttcgt catttctttg agaaactaat tttccagcag tatcccaacg ccttaaagtt
      721 tcttttgaaa cgcccaaaat gtctgcaact tctgaaagtg aaaactcttc ctttaacata
      781 tttagtaacc atgccaacat tagtcatggt tactactgta tgggtttaca tgtgctttgt
      841 caaacattga tcatggttat cagccgtaag cgagcccaaa atacttaata aaactatctt
      901 tatcagggat ggaaaaacct tgctgcccaa gacgttcgct caaaatatac tcaattgcgt
      961 gagggagagc actatggagt tcttgcagag catctggtct gccagtgaca agctcataga
     1021 aactcgcacc atcaatgatt ctaatgttag ggttcgaagg acacagagta ccactacctt
     1081 tattggaagg agtaaagggg ctgttatatc tgataggttt acgagggatt atattaacaa
     1141 agtacgcaca gtaatcctta aatcggctat gtttcggtga taccaactca tctaagcctt
     1201 tatatttatc tgctaaatcc ccgccagtaa ccgttgagta tttatttttc acctcagcga
     1261 tgattcttct ttcttcgttg agaagatcca caggacgggt gtggtcgcca tgatcgcgta
     1321 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
     1381 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
     1441 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
     1501 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     1561 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     1621 accgcattaa agcttctaga ggatcccccc tttcaatttg gcttactcgg atctttaatg
     1681 ctcttgcctg ctcaacaaat ggtgttgctt ttcgatttct ttctaaagct aaagcaacaa
     1741 atcctgctcg gacttcttga tatggagtca caagatcatc attgctttga atatgatatt
     1801 gatatggcac tagggttttc tccaaacata cacacatttg cgaagtgtct ttcttccatg
     1861 tgtccccatt tgttggctac tgttaccttt gccagtaggt aagacaagaa ttttctccac
     1921 attaaaacca atttcttctg caatattaga aagaattaaa tcaaccgaaa tatcaatacc
     1981 tgcatagcga acattatcat ttaccataaa taaaggtgag ccatttttta aaactctaaa
     2041 gcattctata ataacgcaag ccatttcata gaaatatcct tttatcatac gaggaatacc
     2101 gttattatta agtttttttc tttcaagctc tcgctcaaga aagcgcaaga tactttgtaa
     2161 caattcttgt ttatcaagta tgcgtaatgc ttcctgccaa ttgtgaataa gagacttttc
     2221 tttgttttca acagtacaac tcagcatatc ttgcctaagt tgtactatat ctctttctcc
     2281 cacacctaat agagctaatt ctagagcgta tgtacgtgta tagtcataac gattacaata
     2341 gggtggagag gtaatgatac agtcgtaaaa atcttgctca aattcaggca gaattttaag
     2401 acatgaagca ttaaataaat taattccact tctttgagtc ccataacaaa ataatgtatt
     2461 acttattaag tcaaaggaat cattcaaaat taattttatt tgctcagtaa ttgcttcatc
     2521 gaaatccaga attttacctt tatcaaattt atctgatcca cttttcctaa atgctctttt
     2581 atcccaacgt agatactgtc catctttacg ggtatagctg atagattcaa gaatacacaa
     2641 tagagcaaaa cggagaactt gtttcacaag aatattctct ttttctatag aaaataaaaa
     2701 tctctctatt gatgcttctg tttcaggagg ataagctccg tcagtaattc ttaaacgatt
     2761 aagatatttt ctattattat gctgattcca aggtttttgt ttgtaccatt caatcaatct
     2821 caaaaaatct tctcgacgga attgtcgctg gattattttc cttacttcaa taatctcttg
     2881 accaataggt aacacttcta taccatctgc ttgaataccg gcatcactac aggcaaatag
     2941 cattgttcct gctcctgcga aggggtcaag aatcctctca aatgattttt tctcgtactc
     3001 gaatattaac tgatttacca aatcaactga aaaagcttct ttatatttaa accagcgata
     3061 ctgaggtttt gacttattag cttgaaaact aactattttt cgacttaaac tttcatgcaa
     3121 tcgcatctta tctctaaaac gttgctcaag tgctttattg aatcgagaaa tttcttgtac
     3181 aaaggaatca ctctcttgat caagatcagt cgggtcagtc tttatttcta ttggactctc
     3241 aagctcaaat gaagtcattt tatatttttc ttgtaacaaa gaggtaactt aagattctat
     3301 caggacaaag cactatcgtc tatcacttaa gatttgaacc ctatttttta tagactaatt
     3361 gacaacagtt agaacctgat tatactttgt tatacttcta cagtaatcaa gttaaatatt
     3421 gaatagggta ccgagctcga attcatcggt agggagattt tttcaagtaa cagaggtttt
     3481 ggattttaaa aaatatttcc ttgatttcga taaaacgaat aagttcccta tgagctttgt
     3541 tgttaaaact gagcttgagc cagaagaggt tatggacaag ataaagatta actctgaaaa
     3601 aagcatggaa aaggaattgg tgcagaaata tctgattttg tttgaagaaa tttttacatt
     3661 acagacgctc agaagtatcc tgggaatgat atctgactct cttgatgagc ttgaagagct
     3721 aaagagagtc ataatcgaaa attttaaatt agagtatggt cttcttgatt aaataataaa
     3781 tcattgttta atgggttaga gatgttgtca atatcatctc taatgttttt tgatttgttt
     3841 tttgtcttcc ttttttttac tttctccaat tcttttgggg tataatctga aggtataccc
     3901 aatcttctta tgcccatttt tacatactct tcgtttattt ctatgcctat aaatctcctg
     3961 tctagagact tggcaacatc tcctgttgta aagctaccag aaaaagggtc taagactaca
     4021 tcgcctgggt tcgatgaagc tagaataatt ctcctcaaaa gggacgtcgg tttctgtgtg
     4081 gggtggtttt catattcctc cataagaaat cttactctag gaaaatccca aacgtttcct
     4141 ggtacttttt gtgtgctata aggttggggt gggttttttc tataatcaat caaagccctt
     4201 ttagatcctg tttttgcttc aaccaaaata tctttgctgt taaatgtgta ttttttttga
     4261 tcttttacca tcattaagat tggttcgtac atggagccaa agtgtttttt ggcttgaaca
     4321 cctgagctgt cataagacca aactaatcga ctttttatat ggaagatttt tctacatttt
     4381 atgtcaatgt atggcatatt ctcagtgctg ttcataatat acatagtgcc atgctttttt
     4441 agcactctat ggcattcatc gacgcattcg aataaccaat gaaggaattc accctcatcc
     4501 catgactcaa caagtccgtc aaagtcctta ccaatattat atggggggtc tgcaaaaatt
     4561 aaatcaacac tctctgctgg caatttcttt aattcagtca acgcatcgcc atggataatt
     4621 ttattttttt catctttaaa atatctagct tcaaaatcta ttttcatttt ctacctcttg
     4681 tgtgactcat agtctactat gttaccgata tctagatctc gagctatcag gctagagatt
     4741 ttaccgccaa atcgaacctt attagagcgg tttaggctgg accggcagtt aaaattgggg
     4801 cttgagcggt aaacgagtga gggaatttca ggtaagatac ttcggatgag gagcaaaaag
     4861 gtggtttata cttcctatac ccaaaaaggt ggtttatact tcctataccc cggttatccg
     4921 gtggttttct gtttgcggaa taaaacagat tagtgacagt tttctcagaa tggcgtgttc
     4981 cggtgacttc tgttttcgtg tgctgcatcc ggaagtgtca ggcccgccgg atatgaaaaa
     5041 ggcctccgga gagtaccgtg actttatttt cggcacaaat acaggggtcg atggataaat
     5101 acggcgatag tttcctgacg gatgatccgt atgtaccggc ggaagacaag ctgcaaacct
     5161 gtcagatgga gattgattta atggcggatg tgctgagagc accgccccgt gaatccgcag
     5221 aactgatccg ctatgtgttt gcggatgatt ggccggaata aataaagccg ggcttaatac
     5281 agattaagcc cgtatagggt attattactg aataccaaac agcttacgga ggacggaatg
     5341 ttacccattg agacaaccag actgccttct gattattaat atttttcact attaatcaga
     5401 aggaataacc atgaatttta cccggattga cctgaatacc tggaatcgca gggaacactt
     5461 tgccctttat cgtcagcaga ttaaatgcgg attcagcctg accaccaaac tcgatattac
     5521 cgctttgcgt accgcactgg cggagacagg ttataagttt tatccgctga tgatttacct
     5581 gatctcccgg gctgttaatc agtttccgga gttccggatg gcactgaaag acaatgaact
     5641 tatttactgg gaccagtcag acccggtctt tactgtcttt cataaagaaa ccgaaacatt
     5701 ctctgcactg tcctgccgtt attttccgga tctcagtgag tttatggcag gttataatgc
     5761 ggtaacggca gaatatcagc atgataccag attgtttccg cagggaaatt taccggagaa
     5821 tcacctgaat atatcatcat taccgtgggt gagttttgac ggatttaacc tgaacatcac
     5881 cggaaatgat gattattttg ccccggtttt tacgatggca aagtttcagc aggaaggtga
     5941 ccgcgtatta ttacctgttt ctgtacaggt tcatcatgca gtctgtgatg gctttcatgc
     6001 agcacggttt attaatacac ttcagctgat gtgtgataac atactgaaat aaattaatta
     6061 attctgtatt taagccaccg tatccggcag gaatggtggc ttttttttat attttaaccg
     6121 taatctgtaa tttcgtttca gactggttca ggatcactgt acgataatgc ccccgcagtt
     6181 tggtaatacc cttaataaaa aagaaacagc aaagactgac agcaataata ataaagtaag
     6241 cagtaacaat aatattaaca acaccagatg cagttataat aatagtattt aagacaccag
     6301 aaagactgct gcgacagtca ttttgaacaa caccaaaatg ccgtaaaggc agtagtaaca
     6361 acaccagtga aaacatcacg atagcatagt gatatgcctg agtgtgtgta attaaacaat
     6421 aaataaaccg ccatatataa cagaagatag tattctgaat ggcatgcttt tctgttcagt
     6481 ataaacatat catcccggtt ggtataagga tgatatataa taagttaagc tgaacacata
     6541 tttattttgg ttttatttta caaataaagt aagacgatcc gttaagtcaa agcggggtat
     6601 atttattata ccctgctttt ttatttgtcc gccgggcgcg gataatggat cagattatgc
     6661 agtgtcacaa tggccttacc gggattggcg taagcgtgcg ggatatccgc atggaagcgc
     6721 agggattccc cggcagaaac ggtgtgccac tcatccccca gccgcagttg taatgcgcct
     6781 tccagtacaa tgacatgttc tctggttctg aaatccatcc ctgtcggtgt tgcttatgca
     6841 gtctggtcgg gactcggcgt cgtcataatt acagccattg cctggttgct tcatgggcaa
     6901 aagcttgatg cgtggggctt tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc
     6961 cgatccccat cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg ttcggcattc
     7021 tgaatctcac cgaggactcc ttcttcgatg agagccggcg gctagacccc gccggcgctg
     7081 tcaccgcggc gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg
     7141 ccagccatcc ggacgcgagg cctgtatcgc cggccgatga gatcagacgt attgcgccgc
     7201 tcttagacgc cctgtccgat cagatgcacc gtgtttcaat cgacagcttc caaccggaaa
     7261 cccagcgcta tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg
     7321 accctgcgct ctatcccgat attgctgagg cggactgcag atggaatgcg ctttgttacg
     7381 ccagtcagaa caatcaatgc cggaccgaac cgcaaatact ttggtaataa cagagggatc
     7441 acctggtaca actttgtgtc cgatcagtat tccggctttc atggcatcgt tataccgggg
     7501 acgctgaggg actctatctt tgtgctggaa ggccttctgg aacaggagac cgggctgaat
     7561 ccaaccgaaa ttatgaccga tacagcaggt accagcgaac ttgtctttgg ccttttctgg
     7621 ctgctgggat accagttttc tccacgcctg gctgatgccg gtgcttcggt tttctggcga
     7681 atgggccatg atgccaacta tggcgtgctg aatgatattg ccagagggca atcagatccc
     7741 cgaaaaatag tccttcagtg ggacgaaatg atccggaccg ctggctccct gaaactgggc
     7801 aaagtacagg cttcagtgct ggtccgttca ttgctgaaaa gtgaacgtcc ttccggactg
     7861 actcaggcaa tcattgaagt ggggcgcatc aacaaaacgc tgtatctgct taattatatt
     7921 gatgatgaag attaccgccg gcgcattctg acccagctta atcggggaga aagccgccat
     7981 gccgttgcca gagccatctg tcacggtcaa aaaggtgaga taagaaaacg atataccgac
     8041 ggtcaggaag atcaactggg cgcactgggg ctggtcacta acgccgtcgt gttatggaac
     8101 actatgtata tgcaggcagc cctggatcat ctccgggcgc aaggtgaaac actgaatgat
     8161 gaagatatcg cacgcctctc cccgctttgc cacggacata tcaatatgct cggccattat
     8221 tccttcacgc tggcagaact ggtgaccaaa ggacatctga gaccattaaa agaggcgtca
     8281 gaggtagaaa acgttgctta acgtgagttt tcgttccact gagcgtcaga cccctgtaca
     8341 aggaggaacc gttgcaccat cttcgtcttc tgaactgacg gggatcgcgg ttcagtagaa
     8401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg
     8461 aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt cagttgggga ctgcttaacc
     8521 gggtaactgg ctttagtgga gcacagatac caaatgctgt ccttccagtg tagccgctat
     8581 aaggcttcaa cttcaagagt tctgcgtatc taaatccact atccagttac caatggctgc
     8641 tgccagtggc gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8761 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccaaagg
     8821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8881 gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt
     8941 gagcgtcggt ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagctgc
     9001 gtggcctttt ccgggttctt gttttttgct cacatgtttc ttttggtgtt atcccctgat
     9061 tctgtggata accgcattac cgtctttgag tgagctgata ccgctcgccg cagccgaacg
     9121 accgagcgga gcgagtcagt gagcgaggaa gcggaaaagc gcctggacga gcattttctc
     9181 cttacgcatc tgtgcggcat ttcacacccg gcatggtgca cttttcatac aatcctcact
     9241 gatgccaaat gattaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     9301 cgccccgaca cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     9361 ccgctcacag gccagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     9421 tatccccgaa gcgtgcgagg cagctgcggt aaagctcgtc agcgtgatcg tgaagcgctt
     9481 cgcagatgtc agcctggtcg tcggcgtcca gttcattcag tttttccagc agggtcagcg
     9541 tctggcttct gatgaagcgg gccatgttaa gggcggtttt ttcctgttta gtcacagttc
     9601 tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat gataccggtg aaacgcgaaa
     9661 ggatgctcac gatacgggtc acggatgagg aacatgcgcg gctgctggca cgttgtgagg
     9721 gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg ggcaccgcca tcgaaaacgt
     9781 caggtcttcc gacactggca ccgccgctgt tgcgccagtt cgcttcggtc ggcaataacc
     9841 tgaatcagat agcccggaag ataaacagcg gacagtggtc ggggcatgac cgggttcatg
     9901 tggtggctgc actgatggcc atcggacgtg aactgagtga gctgagggat gaggtccgta
     9961 aacagggaga acgggatgat agttaagttt catgccaggg gaaaaggcgg tggcagtggt
    10021 ccggttgatt acctgctggg ccgggagcgt aaccgggaag gggcgagagt tctgcggggt
    10081 gctcccgaag aggtgcggga actcatcgat gccacgccgt ttgcgaaaaa atacacctcg
    10141 ggggttctgt cttttgcaga gcagaccctg ccaccgggag agcgtgaaag ggtgatggag
    10201 agctttgagc gggtactgat gcccggtctt gaaaaaaatc agtacagcat cctgtgggtg
    10261 gagcaccagg acaaggggcg tcttgagctg aattttgtca tcccgaatat ggagctggcg
    10321 agcggaaaac ggctccagcc ctattacgac cgagcggacc gtccgcgaat caatgcctgg
    10381 cagacactgg tgaatcacca ttacgggctg cacgacccga acgcaccgga gaacaggcgg
    10441 acacttgtca ccccgaataa tctgccgaaa gcgaaacagg aggccgcaga agcgattacg
    10501 aggggactgg aggctcttta ccatgccgga gcgctaaaaa cgcgtcagga tgtcactgag
    10561 gcgcttacag cggcaggttt tgaggttgtg aggacaacca ggagcagtat cagtatcgcc
    10621 gacccggagg gtgggcgtaa tctcaggctg aaaggggcgc tgtatgagca atcttttaca
    10681 aatggggaca gacttcgaga agaaactgaa agagcgtgcc gtctctacag agaacatgct
    10741 gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg gaactgaact taaacgggca
    10801 gaaaatcaga gacgctatca gcgcacacac agcagcggtg aaagaacaac tggagacgct
    10861 gaacagtgcc gtgaacgcgc agttcagcgc cacagagaac gaactggccc ggcggcagga
    10921 agagctgctc tggaagctgg tcaaagggcg gatactgtac ccgtcgctga cggcgctttg
    10981 cgtcacgggc gggatttttc tggcgtcatg ggggctgatt cagtggcagg aaagccggat
    11041 tgcggcgaac atcctggcta tcagggacca ggaagagacg ctggcgaaac tccgggaaaa
    11101 aacatggggt gtgacgtatc acgagggcag gaacgggaaa tttctagtgt tgccgtccgg
    11161 ggtgaagggg gaaaacaact ggacggtgga gaagaagaac gcagtaagac tggtcaggga
    11221 gtaagtgagt atgacagagc tggaaatgca cttactgaac gccttagagc aattgcagca
    11281 ggactatatg cagcggctga aagactggga gagcgccttc acggatttgc agcatatgtt
    11341 cgagctgaca cgacaggaga acgggcaact gagggaacag tgcgacgact tgagtcagca
    11401 ggtgcagaga ttagccgggc aggtggacca cttgagtcgg ttatttatca cgaacagcag
    11461 ataagggatg aacaggaaag gcaggcacgc ctggaacggg agcgtgcgcg gtcctggcat
    11521 gaagagcagg aacgggaagc acgggtatgg cgtggcccgt caataggtat gtgacggtga
    11581 gttgttccgg gaggttgcca gatggtaccg gaacgcgtta aaaacgcatc cgggcgcgat
    11641 tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc cagatcgcgc tcattcctca
    11701 gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa gatggaaaaa aaacagtgtc
    11761 atgaggttta cgcaggcaaa accagcgtta ttcacatggc tgaatagcta tgcatacggt
    11821 taaaatttat caggtgcgat cgcggcagct tttcgggtgg tttgttgcca tttttacctg
    11881 tcagcagccg tgatcgcgct gaacgcattt cagcggtgcg tacaattaag ggattatggt
    11941 aaatccctta ctgtctgccc tcgtatccat cgagataaac cgcagaaatg gtgtctgcca
    12001 ttagtttcat ttttttcggc gaaggcatat cgctgtttgt gtattttctg ccagcaaagg
    12061 ctgttgtgct ccggagtgag tgaaaaaatt tttctgagtg gaattctttt acacgttgct
    12121 gtgccgtttc tgctgatact ccggctgctt tgtctttcat ccccatatcc cggaactgct
    12181 gaaagaacgg gagcatcata cgtatggtgt ctgctttctg ggtgtagaag ttaggatcaa
    12241 atccatcatc tgaagcataa acataatgcc gggtatgtcg ttccaggctg gtctttggtg
    12301 tgcatcctgt aagagctatt gttgctgtta tcagaggtat cagaagcagc ctggcagcgg
    12361 cagatctcat aaacatctcc ttgcgttttt aatcttgata ctcagggagt tatatctccg
    12421 aagagtatct cttatcatac gtcggtttac tcccaatgtg atttgatggt caatgagtca
    12481 ggaataatcc cgtgaaaaaa tgtgtgccag tcctgctttt tagtgttctt tctgcttcgg
    12541 ttgccagtca tgcggacagc aatgtttttc aacaaaaggc cgagaagaca aaaaacggca
    12601 ttgttgcgga tgttaatgcc agacagaaaa tgttcagtaa tagactgaag atacaacttc
    12661 agatcgatga gttgagagca gagctggctg gcaccagaga tgcccaaaaa agagaggatt
    12721 tgcgtaaaaa actggagtct ctggagcggc aaaagaaatc cttacgctga tgggggcgta
    12781 ttttccccgg tttcttccat aaccttttta ccgttcagat atttacgggc gaggcgaatg
    12841 cgtcttgcct ggtataccgc cgagtaagcc tgggtgggat tcacaccatt actgatttca
    12901 ctgaccacat gacgctgatc agccagtgtc agcgtgtaaa cctgtttctg gatgaggtca
    12961 cgggctttgt catgctcata tccggcagca acaagggtgt cgagaagtcg tttgtctgtt
    13021 ttgttcagtt tatggcgggg tgatttgctc acatcgttct ccgttttttc atgatccaac
    13081 agttttatat ctcatcctga tttgctccgg tgttctgttt cgacgttctc aatgcctgcc
    13141 tccccatagt gtgatcctta ttgcaaaaat gaaaatccat gctcttgaca tggacaatgc
    13201 tgagtagtag gtttttactg tacataaaac cagtggttat atgtacagta tattgttttt
    13261 aatttattgt tttaattatc aaagaggaat ttatggctaa agaactaagt ggatatggac
    13321 caactgctgg tgagtctatg gggggaacag gggcgaatct gaatcagcaa ggtggaaata
    13381 ataatagtaa tagtggggtt cactggggtg gtggctctgg gcatggtaat aatggtggtc
    13441 agggaaattc aaattcatct ggttctacat caactgtgat gaaaaccggt gagtcatatc
    13501 tgactccgtg gggtgatgtt gtcattaata atgatggcct tccggtgatg aatggtattg
    13561 ttatgacaga agagaattca actctggttg ataatccatt tggaggggtc tccagagttc
    13621 ttaattcttt aattagtgat atgccatcac tttttgcaga aagctcagga aataacaaca
    13681 ataacactgc ttcagttaat actgcaccaa cgaatgctca ggtcagtgat atggataaga
    13741 gttcgaaggt ggtgagtaat gtaattaatg aaaaacaaaa gcagaagaat aaaattgcta
    13801 cacagattag tgaaaaacag aagaaaatcg aggagatgaa aaaagtattc aagcatcatt
    13861 cttatcatgg tatcactgat cttgagaggg atgttgatga gttgcaaaaa aaatcaaatc
    13921 agcttgatgc agatattagc aaacttaatt cttataaaaa tacattgcaa agtaaaattg
    13981 gtgatgtgaa taaacagaag gaagctgaag aaaaggctcg tgaaaatgca gaggtcgctg
    14041 aacatgaaac tcttaatgaa gagaaacagg cggtggcgga agcagagaag cgtttagctg
    14101 aggcgaaagc tgaactggcg aaggctgaaa gtgatgtaca gagtaagcaa gctactgttt
    14161 ccagagttgc aggagagctt gaaaacgctc agaagagtgt tgacgtgaag gtcaccggat
    14221 ttccgggatg gcgtgacgtt cagaaaaaac tacagcgtca gcttgaagct aagcaggctg
    14281 agtactccgc agttgaaaat gaacttaaga atgctgttag tttcagggat ggtaaggccg
    14341 ctgaggttaa agaggcagag cagaaattaa aagaagctca ggatgctctt gaaaagagcc
    14401 agataaaaga tgctgttgat actatggttg gattttatca atatataacc gaacaatatg
    14461 gagaaaaata cgcaaaaatt gctcaggatt tagcggaaaa gtctaaagga aagaaaattc
    14521 aaggtgttga tgaagctctg gcagcatttg aaaagtataa gaatgtactg gataagaaat
    14581 tcagtaaggt tgatcgggat gctattttta atgcgttaga gtctgttaat tatgatgagt
    14641 tatctaagaa tttaacgaag atatcaaagt cgcttaaaat caccagtcga gtatcttttc
    14701 tatatgatgt tggttctgat tttaaaaatg caatagaaac tggtaactgg cgacctttgt
    14761 ttgtcacgct ggagaaaagt gcggtcgatg taggtgtggc aaaaattgtg gcattaatgt
    14821 ttagttttat tgttggtgtg cctcttggtt tctgggggat tgcaattgtc acaggtattg
    14881 tttcttctta cataggggat gatgagttga gcaaacttaa tgagttgtta gggatttaat
    14941 ttctctttag agtgtaatat atgaaacttg gcaatcctac tgggattgcc attaataaat
    15001 aaaacaatgc agataacttg cataagtacc agggttcttt ttttaacttt gtattttctt
    15061 tgatatatct agatatggtt aatttagagt atggatatag caatacatgt attgatgata
    15121 taaatataaa taacaaagga gtgttatcat tgtcgttaaa tattaaaata agaatccatg
    15181 gtataagtga ttcaggtaaa ttatgtaggt agtattttaa atgcataagt atttttactt
    15241 ttgttggaaa gaaatatgaa aataataatt tttctaatta caatattatt cttgtcagga
    15301 tgtcaagcaa actatattcg tgatgtacgg ggtctgacgc tcagtggaac gaaaactcac
    15361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    15421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    15481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    15541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    15601 ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc
    15661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    15721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    15781 ttgccattgc tgcaggtcga ctctagagtc gacgctctcc cttatgcgac tcctgcatta
    15841 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
    15901 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
    15961 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
    16021 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
    16081 agaggatcga actgaacacg tcgcgtgacc gtgaaaacac cttcgaaccg cagttgatta
    16141 agaagcacca gacgcgcatt acgcagatgg acagccagat tttatccctc tacgccaaag
    16201 atatgactac ccgcgaaatt gtcgccacct tcaaagagat gtacgacgca gatgtgtcac
    16261 ccacgctgat atgtaaagtc actgatgcgg tcaaagagca ggttgcagaa tggcaaaatc
    16321 gacctctgga tgcactgtat cccattgttt accttgactg tatcgtggtg aaagttcgtc
    16381 aaggtggcac cgtaattaac aaagcggtgt tccttgcact cggtattaat actggaggcc
    16441 agaaagagtt gctgggcatg tggctggccg agaacgaagg ggcgaagttc tggctggggg
    16501 ggctgacaga gcttaagaat cgggggcttc aggatatcct tattgcctgc atggacggtc
    16561 tgaaaggctt tccggatgcg ataaacagcg tgtatccgta gacgcacatc cagttgtgca
    16621 tcattcatat ggtgcgcaac agcctgaaat acgtatcgtg gaaggactac aaagccgtca
    16681 ctggcgggtt aaaaacggtg tatcaggcgc caacagaaga ggcggcgcta gtggcaccgg
    16741 agcagttccc gaatgtctgg gacgataaat acccgcaaat cagcaaaagc tggcgtgcgc
    16801 actgggaaaa tatcaacacg ttcttcggct attcgcccga tatccgcaag gccatctaca
    16861 ccacgaacgc tatcgaatcg ctgaacagcg tgatccgtgc ggccattaag aagcgtaaag
    16921 tgttcccgac tgacgactcg ctgcggaagg tcatttctct ggcgatcaag gatgcatcaa
    16981 aaaaatggaa tatgccaatc cagagctggc ggctggcgat gagtcgcttt attatcgagt
    17041 tcggtgaccg cctgagcgat cacctttaat atggcagcaa ttacacagaa ttatttacag
    17101 ggtctatctt ttatttatct tatctaaaag atgtaccaca gatttgccaa cagcaaatga
    17161 cagattaaca ggaacagcgt ttcctatttg cttatatttc gctgacattg ggccttcaaa
    17221 tacccattca tcggggaagg tctgtattct tgcatactca cgcacagtta atggtcttgt
    17281 ttcttctggg tggcaacgct ctgtttgttt ctgtgctggg gcgcatgtta atgttaggct
    17341 tggttcatcc catgacaaac gacgagccat accagttttg cccccaccta agtaaaaact
    17401 cttgagcatg tattcttttt gaatatcttc aggaagatct ctccagtagc caccgggagg
    17461 aaccatactt aggatctctg cttttctttt gggatatttt tgtccttcag attctggcac
    17521 gtcgctatca tacagctctc ccttttttaa tgcatct
//
