LOCUS       sequence-42060-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PREX1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             426..443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(496..514)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      510..557
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(681..700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa tattttgttt aactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcaaga acaaacagct tcgcaacgac
      121 ttcaagctgg tggagaacat tctggccaag cgcctgctga tcctgcccca ggaggaggac
      181 tatggctttg acatcgagga gaagaacaag gctgtggtgg tgaagtccgt ccagaggggc
      241 tcgctggctg aggtggctgg cctgcaggtg gggaggaaga tctactccat caatgaggac
      301 ctggtgttcc tgcggccgtt ttcagaggtg gagtccatcc tcaaccagtc cttctgctcc
      361 cgccgccctc tgcgcctcct ggtggccacg aaggcctgat gacgaagctt gcggccgcac
      421 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      481 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      541 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      781 acggcacctc gac
//
