LOCUS       sequence-420635-       11997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, with YFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pMCS-YFP-GW
FEATURES             Location/Qualifiers
     source          1..11997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    1385..1439
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(1441..1443,1444..1446,1447..2157)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1486..1507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1747..1766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2094..2115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2227..2351
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2376..2381,2382..2399,2400..2406)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2460..3119
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2526..2545)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3461..3766
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3645..3664
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3807..3931)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3990..4697
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4722..4739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4722..4738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4731..4753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4941..4965
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6266..6895
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7324..8397
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8463..8657
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8893..8915)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9001..9141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9056..9075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9156..9173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9327..9915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9407..9426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10002..10796)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  10820..11587
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc cctaggagat cttcgaacac gtgaggcctc gagtataaga gctctatttt
     1381 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattacc
     1441 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1501 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1561 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1621 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     1681 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1741 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1801 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1861 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1921 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1981 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2041 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2101 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
     2161 ggactcagat cacgagctca agcttcaaat tctgcagtcg acggtaccgc gggcccggga
     2221 tcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     2281 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     2341 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2401 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     2461 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2521 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2581 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2641 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
     2701 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2761 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2821 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2881 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2941 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3001 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     3061 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     3121 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     3181 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     3241 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     3301 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3361 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3421 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     3481 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3541 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     3601 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3661 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3721 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     3781 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3841 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3901 tttctcgttc agctttcttg tacaaagtgg ttgatgatcc taggtgagtc tagagagtta
     3961 attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg
     4021 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
     4081 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
     4141 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
     4201 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
     4261 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
     4321 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
     4381 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
     4441 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
     4501 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
     4561 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
     4621 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
     4681 atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
     4741 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4801 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4861 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
     4921 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     4981 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     5041 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     5101 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     5161 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     5221 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     5281 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     5341 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     5401 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     5461 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     5521 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     5581 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     5641 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     5701 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     5761 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     5821 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     5881 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     5941 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     6001 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     6061 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     6121 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     6181 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     6241 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     6301 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     6361 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     6421 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     6481 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     6541 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     6601 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     6661 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     6721 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     6781 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     6841 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     6901 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     6961 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     7021 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     7081 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     7141 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     7201 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     7261 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     7321 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     7381 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     7441 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     7501 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     7561 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     7621 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     7681 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     7741 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     7801 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     7861 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     7921 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     7981 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     8041 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     8101 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     8161 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     8221 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     8281 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     8341 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     8401 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     8461 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     8521 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     8581 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     8641 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     8701 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     8761 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     8821 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     8881 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     8941 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9001 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     9061 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9121 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9961 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
    10021 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    10081 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    10141 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10201 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10261 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    10321 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    10381 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    10441 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    10501 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    10561 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    10621 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    10681 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    10741 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    10801 tatttccttc ctcttttctg gtaatgactc caacttattg atagtgtttt atgttcagat
    10861 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
    10921 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
    10981 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
    11041 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
    11101 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
    11161 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
    11221 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
    11281 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
    11341 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
    11401 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
    11461 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
    11521 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
    11581 catcaccctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    11641 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    11701 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    11761 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    11821 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    11881 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    11941 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctgg
//
