LOCUS       sequence-420639-       12777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis UBQ10 promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 12777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pUBQ10-YFP-GW
FEATURES             Location/Qualifiers
     source          1..12777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    2942..2996
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(2998..3000,3001..3003,3004..3714)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3043..3064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3304..3323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3651..3672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3784..3908
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3933..3938,3939..3956,3957..3963)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4017..4676
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4083..4102)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5018..5323
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5202..5221
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5364..5488)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5547..6254
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6279..6296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6279..6295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6498..6522
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7823..8452
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8881..9954
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10020..10214
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10450..10472)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10558..10698
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10613..10632
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10713..10730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10884..11472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10964..10983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11559..12353)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc gtatgatcgc gaagcaccca ccctaagcca cattttaatc cttctgttgg
     1381 ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg
     1441 tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa
     1501 tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc
     1561 ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag
     1621 cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg
     1681 tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga
     1741 atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg
     1801 actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt
     1861 aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac
     1921 caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata
     1981 acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac
     2041 ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa
     2101 acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg
     2161 cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata
     2221 aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct
     2281 tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg
     2341 ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac
     2401 ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg
     2461 tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg
     2521 tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca
     2581 gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt
     2641 aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt
     2701 tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt
     2761 tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt
     2821 caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg
     2881 gtgttagttt ctagtttgtg cgatcgaatt tgtcctcgag tataagagct ctatttttac
     2941 aacaattacc aacaacaaca aacaacaaac aacattacaa ttacatttac aattaccatg
     3001 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3061 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3181 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     3241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     3481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     3541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3601 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
     3721 ctcagatcac gagctcaagc ttcaaattct gcagtcgacg gtaccgcggg cccgggatca
     3781 tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     3841 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     3901 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     3961 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     4021 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     4081 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     4141 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     4201 ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc
     4261 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     4321 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     4381 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     4441 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     4501 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     4561 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     4621 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat
     4681 ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     4741 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     4801 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     4861 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     4921 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     4981 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     5041 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     5101 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     5161 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     5221 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     5281 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     5341 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     5401 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     5461 ctcgttcagc tttcttgtac aaagtggttg atgatcctag gtgagtctag agagttaatt
     5521 aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
     5581 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
     5641 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
     5701 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
     5761 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
     5821 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
     5881 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     5941 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     6001 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     6061 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     6121 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     6181 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     6241 tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     6301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6421 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
     6481 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     6541 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     6601 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     6661 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     6721 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     6781 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     6841 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     6901 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     6961 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     7021 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     7081 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     7141 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     7201 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     7261 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     7321 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     7381 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     7441 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     7501 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     7561 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     7621 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     7681 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     7741 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     7801 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     7861 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     7921 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     7981 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     8041 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     8101 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     8161 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     8221 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     8281 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     8341 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     8401 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     8461 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     8521 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     8581 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     8641 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     8701 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     8761 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     8821 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     8881 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     8941 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     9001 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     9061 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     9121 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     9181 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     9241 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     9301 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     9361 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     9421 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     9481 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     9541 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     9601 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     9661 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     9721 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     9781 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     9841 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     9901 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     9961 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
    10021 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    10081 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    10141 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    10201 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    10261 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    10321 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    10381 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    10441 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10501 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    10561 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    10621 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10681 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11521 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
    11581 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    11641 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    11701 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    11761 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    11821 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    11881 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    11941 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    12001 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    12061 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    12121 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    12181 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    12241 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    12301 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    12361 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    12421 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    12481 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    12541 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    12601 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    12661 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    12721 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctgg
//
