LOCUS       sequence-420641-       14059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Preparatory plasmid for gene disruption into the genome using 
            CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14059)
  TITLE     Harnessing xylitol utilization in Escherichia coli for 
            antibiotic-free plasmid and recombinant protein production.
REFERENCE   2  (bases 1 to 14059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: INT_GFPsp_CamRcargo
FEATURES             Location/Qualifiers
     source          1..14059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(345..363)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      359..406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             561..1220
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(627..646)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(1420..1437)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1420..1436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1429..1451)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(1583..2533)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2581..2803
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(3455..4270)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3455..4270)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4178..4197
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4262..4281
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4693..4710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4721..4755
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             5133..5155
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(5163..5165,5166..5879)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5163..5165,5166..5876)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5205..5226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5466..5485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5813..5834
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             12958..12969
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagccaacg aaagtttgca gaaaaaattg tatgcattct ctcagggaaa catgcgttcg
       61 ttgagaaacc ttatttatca agcatctatc gaagcaattg ataatcagca tgagacgata
      121 accgaagaag atttcgtttt tgcatcgaag ttgacatcgg gcgataaacc caactcatgg
      181 aaaaatcctt ttgaggaggg tgttgaggta acagaagata tgttacgacc gccaccaaaa
      241 gatattggtt gggaggacta tttgagacat tcaaccccga gagtgagtaa accaggtaga
      301 aataaaaact ttttcgaata acctaggctg ctgcgctgct gccaccgctg agcaataact
      361 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
      421 atttgttgtt gatacaacca taaaatgata attacaccca taaattgata attatcacac
      481 ccataaattg atattgcctc ttcatggtct aaacttcagt aagtttacga cattttcctc
      541 gaggtcattt ccggggatcc atggagaaaa aaatcactgg atataccacc gttgatatat
      601 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      661 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      721 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggagttcc
      781 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      841 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      901 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      961 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     1021 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg
     1081 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     1141 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     1201 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttct agagtcctta
     1261 ctgcagtagt tttgctgaaa tactcgattc acaaaaatat caacttatgg ttgttttgtg
     1321 agatatcaat atatggttgt tttgtggtta agttgctgat tataaataat tattaaatat
     1381 cactttatgg ttgcatcaac aggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     1441 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcggaca gttttccctt
     1501 tgatatgtaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat
     1561 agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg
     1621 tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca
     1681 tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt
     1741 gtagtgtttt tcttagtccg ttatgtaggt aggaatctga tgtaatggtt gttggtattt
     1801 tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat
     1861 ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt
     1921 tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc
     1981 ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa
     2041 tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa
     2101 tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt
     2161 tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc
     2221 ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc
     2281 tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat
     2341 tttccctact gatgttcatc atctgaacgt attggttata agtgaacgat accgtccgtt
     2401 ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct
     2461 tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa
     2521 ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac caattgagat
     2581 gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct
     2641 gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac
     2701 ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat
     2761 aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc
     2821 cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa
     2881 accctaaagg cttaagtagc accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg
     2941 ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat
     3001 tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct
     3061 tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc
     3121 agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt
     3181 cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac
     3241 tggctaatgc acccagtaag gcagcggtat catcaacggg gtctgacgct cagtggaacg
     3301 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3361 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3421 acagttacgt ttccaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     3481 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     3541 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     3601 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     3661 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     3721 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     3781 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     3841 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     3901 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     3961 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     4021 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     4081 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     4141 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     4201 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     4261 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     4321 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     4381 ccctgcaggg tttgcagtca gagtagaata gaagtatcaa aaaaagcacc gactcggtgc
     4441 cactttttca agttgataac ggactagcct tattttaact tgctatgctg tttgtgatgg
     4501 cttccatgtc ggcagaatgc ttaatgaatt acaacagttt ttatgcatgc gcccaatacg
     4561 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4621 cgactggtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4681 acgaccgagc gcagcgagtc agtgagcgag gacacaagct ttgacagcta gctcagtcct
     4741 aggtataata ctagcgtcga cgtggagata taccatgggt gaactgccga gtaggtagct
     4801 gataacgaga ccccgcagca gggaaataat tcccgccaaa tagcttttta tcacgcaaat
     4861 aatttgtggt gatctacact gatactctgt tgcattattc gcctgaaacc acaatattca
     4921 ggcgtttttt cgctatcttt gacaaaaaat atcaactttc tcgatttgct ctcagccctt
     4981 atatcacggg aaattccggc gatttgctcg catcaatatt catgccacat ttgccatcag
     5041 gggttgcctc agattctcag tatgttaggg tagaaaaaag tgactatttc cattgggtaa
     5101 tatatcgaca tagacaaata aatagaaata attttgttta actttaagaa ggagatatac
     5161 atatgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5221 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     5281 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5341 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     5401 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     5461 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     5521 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     5581 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     5641 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     5701 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5761 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5821 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgat
     5881 gggctgaagg gtttaagcga agacgaacaa taaggcctcc caaatcgggg ggcctttttt
     5941 attgataaca aaaaggcaac actatgacat cggaaaaccc gttactggcg ctgcgagaga
     6001 aaatcagcgc gctggatgaa aaattattag cgttactggc agagcggcgc gaaggtctcg
     6061 tgaactgccg agtaggtagc tgataacgga tccgaattcg agcgaaggag atatacatat
     6121 gtttttgcaa agacctaaac cttacagcga tgaaagttta gaaagtttct ttatccgagt
     6181 ggctaacaaa aatggctacg gtgatgtcca tcgcttccta gaagccacta aacgattcct
     6241 tcaagacatt gaccataatg gctatcaaac ctttccgact gatataactc ggataaaccc
     6301 atactcagct aaaaacagtt ccagcgcacg aactgcgtca ttcctgaaac ttgcacaatt
     6361 gacatttaat gaaccgccag agctacttgg gttggcaatt aacagaacaa acatgaaata
     6421 ctcgccgtca actagcgcgg ttgttagagg tgcagaagtc tttcctcgca gtttactacg
     6481 gacgcactcc atcccctgct gtcctttgtg tctgcgagaa aatggctacg cctcctacct
     6541 ttggcacttt caggggtacg aatactgcca cagccataac gtacctttaa ttaccacttg
     6601 tagctgtggt aaggagtttg actaccgagt atctgggtta aagggcattt gctgcaaatg
     6661 caaggagcct atcaccttaa ccagcaggga gaacggtcat gaggcagcgt gtactgtttc
     6721 aaactggctt gctggccatg aatctaaacc tctgccaaat cttcctaaaa gctaccgatg
     6781 gggtttagtt cattggtgga tgggtattaa agatagcgag ttcgatcact tttcgttcgt
     6841 tcaatttttc tcaaactggc caaggtcatt ccactcgata atcgaagatg aagtagagtt
     6901 caaccttgag catgctgttg tcagcacgtc tgaattacga ctaaaagatc ttcttggtcg
     6961 attgtttttc ggttcaattc ggttacctga gcggaatctt caacacaata tcatccttgg
     7021 tgagcttctc tgctatttag aaaatcgttt atggcaagac aagggattaa tcgccaacct
     7081 caaaatgaac gcgttagagg cgactgtaat gttaaattgt agcctcgatc agattgcatc
     7141 aatggttgaa caacgcatct tgaagccaaa tcgaaaaagc aagcccaaca gccctcttga
     7201 tgttaccgat tatctatttc atttcggcga tattttctgt ctttggttag ctgagttcca
     7261 aagcgatgag tttaaccgtt cgttttatgt gtcgaggtgg taaatgcaaa ctctgaaaga
     7321 actaatcgca tccaatcctg acgacttaac aactgaactt aagagagcat ttcgtccact
     7381 cacaccgcat attgcaattg atggtaatga acttgacgca ctgacgatat tagtcaattt
     7441 aaccgataag actgatgatc agaaagacct gctcgatcga gccaaatgca agcaaaaact
     7501 tcgagatgaa aaatggtggg ctagctgcat aaattgcgtt aattacagac aaagccataa
     7561 cccaaaattc ccggatatac gttctgaagg cgtgattcga acccaagccc tgggtgaatt
     7621 accgagtttc ctactctcgt cctcaaaaat cccaccatac cattggtcat atagccatga
     7681 ttcaaaatac gtcaacaaaa gcgcattcct caccaatgag ttttgttggg atggtgagat
     7741 ctcatgtttg ggtgagcttc ttaaagatgc agatcaccca ctttggaata ctttgaaaaa
     7801 gttaggttgt tctcaaaaaa cttgcaaagc aatggcaaaa caactagctg atattactct
     7861 cacgactatc aatgtcacac tcgcaccaaa ttacctgact caaatatctc tccccgatag
     7921 tgatacatcc tacatttcac tctctcctgt tgcatcgcta tcgatgcaaa gccactttca
     7981 tcagaggctc caagatgaaa atcggcatag cgcgataacg cggtttagcc gaactaccaa
     8041 catgggggtc actgcgatga catgtggcgg cgcatttaga atgttaaagt ctggcgctaa
     8101 gttttccagc ccccctcatc accgattaaa cagtaaacga agttggttga cgtcagagca
     8161 tgttcagtca ttaaaacagt accagcgcct caacaaaagc ctcatacctg aaaactctcg
     8221 gattgcactc cgtagaaaat acaaaatcga gcttcaaaat atggtcagat cttggtttgc
     8281 aatgcaagac catacccttg attcgaacat acttatccaa catttgaatc atgacctatc
     8341 ttacttagga gccacaaaac gttttgcata cgatccagcg atgaccaagc tctttactga
     8401 gcttttgaaa cgagagttat caaattcaat caataatggt gagcaacaca ctaatggatc
     8461 gtttttagtc ctaccgaata tcagggtttg tggcgcaaca gctttaagct ccccggtaac
     8521 ggtggggatt ccatcactta cagctttctt tggcttcgtt cacgcatttg aacggaatat
     8581 aaatcgcacc acctcatcgt ttcgtgttga atcctttgcg atatgcgtcc atcaactaca
     8641 tgtcgagaag cgaggtttga cagcagagtt tgtggaaaaa ggcgacggga ctatatccgc
     8701 tcccgcgacc cgggatgact ggcagtgtga tgtcgtattt agccttattt tgaacaccaa
     8761 ctttgctcaa catattgacc aagatacgtt agttacatca ctaccaaagc gattggctcg
     8821 gggttcagca aaaattgcga ttgatgactt taaacatatc aactcattct cgacattaga
     8881 aacagcgatc gaatctctgc caatagaagc tggtaggtgg ttatcacttt acgcacagtc
     8941 aaacaataat ctaagtgatc tattagcagc catgacagag gaccatcagc tcatggcaag
     9001 ctgcgtcggt taccacttgt tagaagagcc caaagataaa ccaaactccc tcagaggtta
     9061 caaacacgct atcgccgagt gcatcattgg actcattaac tcaatcacct ttagctcaga
     9121 gactgatccc aacacaatct tttggtcgct aaagaactat caaaactacc tagtggtaca
     9181 gccaaggagt atcaacgatg aaactaccga caaatctagc ctatgagcgc tctatcgacc
     9241 catcagatgt ctgttttttt gtcgtctggc ccgatgatag aaaaacacct ttaacctaca
     9301 attctcgtac tctgctcggg caaatggaag cggcatcatt agcctatgat gtctcaggtc
     9361 aaccaataaa aagtgccacc gctgaggcgt tagctcaagg gaaccctcat caagttgatt
     9421 tctgccacgt tccatacggt gcgagtcata ttgaatgcag tttctccgtc tcgttttctt
     9481 ctgaactacg tcaaccatat aagtgtaact caagcaaagt taaacaaacg ctagtgcaat
     9541 tagtcgagct ctacgaaacg aaaatcggct ggactgagct agcaacccga tatttgatga
     9601 acatttgcaa cggtaaatgg ctgtggaaaa atacccgtaa agcctattgc tggaacattg
     9661 tacttacacc ttggccttgg aacggggaaa aggttggatt tgaagatatt cgtactaact
     9721 acacctcacg gcaagacttt aaaaataata aaaattggtc tgctatagtt gaaatgatca
     9781 aaaccgcatt ttctagtact gatgggctgg cgatatttga agtcagggcc accttgcact
     9841 tgccaacgaa tgctatggtg cggccaagcc aagttttcac agaaaaagaa agtggcagta
     9901 aaagtaaatc taaaactcaa aacagtcgag tttttcagag tacaactatt gatggtgaac
     9961 gatcgccaat actaggggcc tttaaaacgg gagcagctat tgcaaccatt gacgactggt
    10021 atcctgaagc cactgagcca ctaagggtcg gacggtttgg ggttcatcgc gaagatgtca
    10081 cttgctaccg tcatccgtct accggaaaag attttttctc gatattacaa caagcagagc
    10141 actatattga agtgttgagc gccaacaaaa ctcccgctca agaaactatc aacgacatgc
    10201 actttttaat ggctaacctg attaagggtg ggatgttcca gcataaagga gactgactgt
    10261 gaaatggtat tataagacaa tcacctttct gccagagttg tgcaacaacg agtcactggc
    10321 tgcaaagtgt ctccgcgttc tgcatggatt taactatcag tatgagacac gaaatatcgg
    10381 cgtttcattt ccgctttggt gtgatgcaac ggttggaaaa aagatttcat ttgtcagcaa
    10441 gaacaagata gaactcgact tactacttaa acaacactat ttcgtccaaa tggaacaact
    10501 tcaatacttt catatatcca acactgttct cgtcccagaa gattgtacat acgtttcctt
    10561 tagacgctgt caatctatag ataagctcac agcagcaggg ctggcaagga aaatcagacg
    10621 cctggagaaa cgtgctctat ctagaggcga gcaatttgac ccatcatctt ttgctcaaaa
    10681 agagcatact gcaatagcgc actaccactc acttggggag tccagcaaac agacgaaccg
    10741 caactttcga ctcaatatca ggatgctctc ggagcaaccc cgtgagggga actcgatttt
    10801 tagtagctat ggcttatcaa attcagaaaa ctcgtttcag cctgtaccct taatctgacc
    10861 tttaataagg agatatacca tggcgacaag tttacctacg ccctcagcaa ttacgacttc
    10921 ggcgttagag tatgcattcc atactcccgc tcgcaatcta acgaaatctc gcggaaaaaa
    10981 cattcatcgt tatgtcagtg taaagatgag taagaggatt acggtagaat ctactctaga
    11041 gtgtgatgcc tgctatcact ttgattttga gccaagtatt gttcgctttt gcgctcaacc
    11101 gattcgattt ttatattatc tcaatggtca gtctcactcc tatgttcctg actttctagt
    11161 tcaatttgat accaacgagt ttgttctata tgaagtaaag tcagcttatg ctaagaacaa
    11221 acctgatttt gatgttgaat gggaggcgaa agtaaaagca gcaactgaac tagggctaga
    11281 attggagctt gttgaagaga gtgatattag ggatacggtt gtattaaata atcttaagcg
    11341 catgcatcgt tatgcttcga aagatgagct gaataacgta cataactctc tcttaaaaat
    11401 aataaagtac aatggcgccc aatctgcaag atgcttggga gaacagttgg gtttaaaagg
    11461 ccgaactgtt ttaccaattt tgtgcgattt gctgtcaagg tgtttactcg atacacgttt
    11521 ggataagcct ctatctcttg aatctcgatt tgagttggcc agttatggct aagaaagggt
    11581 tctcaagttt ccatagaaaa gcagtctctt cccaagatac gcttgaatcg atagagcttg
    11641 tctctagcgc taattgtcta gaaagtgtta cgtatcaaga tatatcagca tttcccgaaa
    11701 caattgcggt agagattaat ttccgattaa gcattcttcg ctttttagcg cgaaagtgcg
    11761 aaaccattgt ggccaaatca attgaaccac atcgtgtaga gctacagcaa aactatagta
    11821 gaaaaatacc cagtgcaata acgatatatc gatggtggct tgcttttcga aaatcagact
    11881 acaaccccat tagcttagca cctaatatca aggatagagg taatagagag acaaaagtgt
    11941 caacagttgt tgattctatt atggaacagg cagttgaaag agttatatct ggacgaaaag
    12001 tcaatgttag ctctgcatat aaacgtgttc gacgaaaagt tcgtcaatac aatctaactc
    12061 atggaacgaa atacacgtat cctaagtacg aatctgtaag aaagcgagta aaaaagaaaa
    12121 ccccatttga gttattagcc gcagggaaag gggagagagt agctaagaga gagtttcgcc
    12181 gaatgggaaa aaagatcctc acgtctagcg tgctagagag ggttgaaata gatcacactg
    12241 tcgttgacct ttttgcagta cacgaagagt atcgaatccc attgggccga ccttggctta
    12301 ctcaattggt tgattgttac agtaaagctg taatcggttt ttatttaggt ttcgagcctc
    12361 ctagctatgt gtcggtttcc cttgcactta agaatgcaat acaacgcaaa gatgacttaa
    12421 tctcctcgta tgaatcgatc gagaatgaat ggctatgtta tggcatccca gacctactcg
    12481 taactgataa tggtaaagag tttttgtcga aagcatttga tcaagcatgt gaatcactat
    12541 tgatcaatgt gcatcaaaat aaagttgaga cgcccgacaa caaacctcat gttgaacgta
    12601 actacgggac tattaatact tctctgttag acgatttacc tgggaaatcc ttcagccagt
    12661 accttcaaag agaagggtac gactctgtgg gagaagctac ccttacactc aatgagatta
    12721 gagaaattta cttaatttgg ttggtggata tttatcataa aaaacccaat cagagaggca
    12781 ctaattgtcc taatgttgcg tggaaaaagg gttgtcaaga atgggaacca gaggagttct
    12841 ctggttctaa agacgaatta gactttaaat ttgctattgt tgattacaaa caacttacta
    12901 aagtagggat aactgtctac aaagaactga gttatagcaa tgaccgttta gctgaatata
    12961 gagggaagaa aggaaaccat aaagttcagt tcaagtataa ccctgagtgt atggcagtta
    13021 tttgggtgtt ggatgaggat atgaatgagt actttacagt taatgcgatt gactacgaat
    13081 atgcaagtag agtatcactt tggcaacata aatataacat gaaatatcaa gcagaactaa
    13141 attcagcaga atatgatgag gacaaggaaa ttgatgcaga aataaaaatt gaagaaatcg
    13201 cagatcgttc aattgttaag actaacaaaa tcagagctcg gaggcgtggc gctaggcatc
    13261 aagagaatag cgcaagggct aagtcaatca gtaatgcgaa cccggcctcg atacaaaaac
    13321 atgaagatga aatcgttagt gcagataatg acgattggga tattgattat gtctgagaaa
    13381 tcgtcaaatg agtgaaacgc gtgaggctcg tatatcaaga gctaaaaggg catttgtatc
    13441 cacaccgagc gttaggaaaa tcttgagtta catggataga tgtagagatc tatcagacct
    13501 agagtctgag cctacatgca tgatggtcta tggtgcttcg ggtgtaggta aaacgaccgt
    13561 catcaagaaa tacttaaatc aaaacagaag agagtccgaa gccgggggcg atataatacc
    13621 ggttttgcat attgagttgc cagacaatgc gaagccagta gatgcagcaa gggaattgct
    13681 ggttgaaatg ggtgacccgc tagcacttta tgaaactgac ttagctagat tgacgaaaag
    13741 actgactgaa ttaatccctg cggtcggcgt gaagctgatt attatcgatg agttccaaca
    13801 tttggtggaa gaaaggtcaa atcgggttct tacccaagta ggtaattggc taaaaatgat
    13861 acttaacaaa acgaaatgtc caattgttat atttggtatg ccatactcaa aagttgtact
    13921 gcaagcaaac tcgcaacttc acgggcgatt ttccattcag gttgaactgc gcccctttag
    13981 ctaccaggga ggtagaggtg tatttaaaac ttttttggaa taccttgata aagccctacc
    14041 ttttgaaaaa caggctggc
//
