LOCUS       sequence-420643-       11845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Effector plasmid for for operon integration into the genome (Safe 
            Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating 
            cells at 37 C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11845)
  TITLE     Harnessing xylitol utilization in Escherichia coli for 
            antibiotic-free plasmid and recombinant protein production.
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: INT_SS9sp
FEATURES             Location/Qualifiers
     source          1..11845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(345..363)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      359..406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(429..446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(429..445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(438..460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(592..1542)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1590..1812
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(2464..3279)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2464..3279)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3187..3206
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3271..3290
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3702..3719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3730..3764
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             10744..10755
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagccaacg aaagtttgca gaaaaaattg tatgcattct ctcagggaaa catgcgttcg
       61 ttgagaaacc ttatttatca agcatctatc gaagcaattg ataatcagca tgagacgata
      121 accgaagaag atttcgtttt tgcatcgaag ttgacatcgg gcgataaacc caactcatgg
      181 aaaaatcctt ttgaggaggg tgttgaggta acagaagata tgttacgacc gccaccaaaa
      241 gatattggtt gggaggacta tttgagacat tcaaccccga gagtgagtaa accaggtaga
      301 aataaaaact ttttcgaata acctaggctg ctgcgctgct gccaccgctg agcaataact
      361 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
      421 atttgttcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      481 cttaatcgcc ttgcggacag ttttcccttt gatatgtaac ggtgaacagt tgttctactt
      541 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
      601 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
      661 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
      721 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
      781 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
      841 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
      901 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
      961 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     1021 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     1081 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     1141 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     1201 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     1261 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     1321 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgaacgta
     1381 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     1441 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     1501 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     1561 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     1621 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     1681 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     1741 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     1801 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     1861 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     1921 ctcggttgcg gccgcaatcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg
     1981 cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg
     2041 aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata
     2101 atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt
     2161 gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt
     2221 cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc
     2281 atcaacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2401 ctaaagtata tatgagtaaa cttggtctga cagttacgtt tccaaccaat taaccaattc
     2461 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     2521 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     2581 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     2641 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     2701 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     2761 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     2821 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     2881 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2941 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     3001 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3061 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3121 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     3181 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     3241 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     3301 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     3361 tcagagattt tgagacacaa cgtggctttc cctgcagggt ttgcagtcag agtagaatag
     3421 aagtatcaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt
     3481 attttaactt gctatgctgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     3541 caacagtttt tatgcatgcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3601 cattaatgca gctggcacga caggtttccc gactggtgga taaccgtatt accgcctttg
     3661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3721 acacaagctt tgacagctag ctcagtccta ggtataatac tagcgtcgac gtggagatat
     3781 accatgggtg aactgccgag taggtagctg ataatctggc gcagttgata tgtaaggcag
     3841 gtttattgaa ctgccgagta ggtagctgat aacggatccg aattcgagcg aaggagatat
     3901 acatatgttt ttgcaaagac ctaaacctta cagcgatgaa agtttagaaa gtttctttat
     3961 ccgagtggct aacaaaaatg gctacggtga tgtccatcgc ttcctagaag ccactaaacg
     4021 attccttcaa gacattgacc ataatggcta tcaaaccttt ccgactgata taactcggat
     4081 aaacccatac tcagctaaaa acagttccag cgcacgaact gcgtcattcc tgaaacttgc
     4141 acaattgaca tttaatgaac cgccagagct acttgggttg gcaattaaca gaacaaacat
     4201 gaaatactcg ccgtcaacta gcgcggttgt tagaggtgca gaagtctttc ctcgcagttt
     4261 actacggacg cactccatcc cctgctgtcc tttgtgtctg cgagaaaatg gctacgcctc
     4321 ctacctttgg cactttcagg ggtacgaata ctgccacagc cataacgtac ctttaattac
     4381 cacttgtagc tgtggtaagg agtttgacta ccgagtatct gggttaaagg gcatttgctg
     4441 caaatgcaag gagcctatca ccttaaccag cagggagaac ggtcatgagg cagcgtgtac
     4501 tgtttcaaac tggcttgctg gccatgaatc taaacctctg ccaaatcttc ctaaaagcta
     4561 ccgatggggt ttagttcatt ggtggatggg tattaaagat agcgagttcg atcacttttc
     4621 gttcgttcaa tttttctcaa actggccaag gtcattccac tcgataatcg aagatgaagt
     4681 agagttcaac cttgagcatg ctgttgtcag cacgtctgaa ttacgactaa aagatcttct
     4741 tggtcgattg tttttcggtt caattcggtt acctgagcgg aatcttcaac acaatatcat
     4801 ccttggtgag cttctctgct atttagaaaa tcgtttatgg caagacaagg gattaatcgc
     4861 caacctcaaa atgaacgcgt tagaggcgac tgtaatgtta aattgtagcc tcgatcagat
     4921 tgcatcaatg gttgaacaac gcatcttgaa gccaaatcga aaaagcaagc ccaacagccc
     4981 tcttgatgtt accgattatc tatttcattt cggcgatatt ttctgtcttt ggttagctga
     5041 gttccaaagc gatgagttta accgttcgtt ttatgtgtcg aggtggtaaa tgcaaactct
     5101 gaaagaacta atcgcatcca atcctgacga cttaacaact gaacttaaga gagcatttcg
     5161 tccactcaca ccgcatattg caattgatgg taatgaactt gacgcactga cgatattagt
     5221 caatttaacc gataagactg atgatcagaa agacctgctc gatcgagcca aatgcaagca
     5281 aaaacttcga gatgaaaaat ggtgggctag ctgcataaat tgcgttaatt acagacaaag
     5341 ccataaccca aaattcccgg atatacgttc tgaaggcgtg attcgaaccc aagccctggg
     5401 tgaattaccg agtttcctac tctcgtcctc aaaaatccca ccataccatt ggtcatatag
     5461 ccatgattca aaatacgtca acaaaagcgc attcctcacc aatgagtttt gttgggatgg
     5521 tgagatctca tgtttgggtg agcttcttaa agatgcagat cacccacttt ggaatacttt
     5581 gaaaaagtta ggttgttctc aaaaaacttg caaagcaatg gcaaaacaac tagctgatat
     5641 tactctcacg actatcaatg tcacactcgc accaaattac ctgactcaaa tatctctccc
     5701 cgatagtgat acatcctaca tttcactctc tcctgttgca tcgctatcga tgcaaagcca
     5761 ctttcatcag aggctccaag atgaaaatcg gcatagcgcg ataacgcggt ttagccgaac
     5821 taccaacatg ggggtcactg cgatgacatg tggcggcgca tttagaatgt taaagtctgg
     5881 cgctaagttt tccagccccc ctcatcaccg attaaacagt aaacgaagtt ggttgacgtc
     5941 agagcatgtt cagtcattaa aacagtacca gcgcctcaac aaaagcctca tacctgaaaa
     6001 ctctcggatt gcactccgta gaaaatacaa aatcgagctt caaaatatgg tcagatcttg
     6061 gtttgcaatg caagaccata cccttgattc gaacatactt atccaacatt tgaatcatga
     6121 cctatcttac ttaggagcca caaaacgttt tgcatacgat ccagcgatga ccaagctctt
     6181 tactgagctt ttgaaacgag agttatcaaa ttcaatcaat aatggtgagc aacacactaa
     6241 tggatcgttt ttagtcctac cgaatatcag ggtttgtggc gcaacagctt taagctcccc
     6301 ggtaacggtg gggattccat cacttacagc tttctttggc ttcgttcacg catttgaacg
     6361 gaatataaat cgcaccacct catcgtttcg tgttgaatcc tttgcgatat gcgtccatca
     6421 actacatgtc gagaagcgag gtttgacagc agagtttgtg gaaaaaggcg acgggactat
     6481 atccgctccc gcgacccggg atgactggca gtgtgatgtc gtatttagcc ttattttgaa
     6541 caccaacttt gctcaacata ttgaccaaga tacgttagtt acatcactac caaagcgatt
     6601 ggctcggggt tcagcaaaaa ttgcgattga tgactttaaa catatcaact cattctcgac
     6661 attagaaaca gcgatcgaat ctctgccaat agaagctggt aggtggttat cactttacgc
     6721 acagtcaaac aataatctaa gtgatctatt agcagccatg acagaggacc atcagctcat
     6781 ggcaagctgc gtcggttacc acttgttaga agagcccaaa gataaaccaa actccctcag
     6841 aggttacaaa cacgctatcg ccgagtgcat cattggactc attaactcaa tcacctttag
     6901 ctcagagact gatcccaaca caatcttttg gtcgctaaag aactatcaaa actacctagt
     6961 ggtacagcca aggagtatca acgatgaaac taccgacaaa tctagcctat gagcgctcta
     7021 tcgacccatc agatgtctgt ttttttgtcg tctggcccga tgatagaaaa acacctttaa
     7081 cctacaattc tcgtactctg ctcgggcaaa tggaagcggc atcattagcc tatgatgtct
     7141 caggtcaacc aataaaaagt gccaccgctg aggcgttagc tcaagggaac cctcatcaag
     7201 ttgatttctg ccacgttcca tacggtgcga gtcatattga atgcagtttc tccgtctcgt
     7261 tttcttctga actacgtcaa ccatataagt gtaactcaag caaagttaaa caaacgctag
     7321 tgcaattagt cgagctctac gaaacgaaaa tcggctggac tgagctagca acccgatatt
     7381 tgatgaacat ttgcaacggt aaatggctgt ggaaaaatac ccgtaaagcc tattgctgga
     7441 acattgtact tacaccttgg ccttggaacg gggaaaaggt tggatttgaa gatattcgta
     7501 ctaactacac ctcacggcaa gactttaaaa ataataaaaa ttggtctgct atagttgaaa
     7561 tgatcaaaac cgcattttct agtactgatg ggctggcgat atttgaagtc agggccacct
     7621 tgcacttgcc aacgaatgct atggtgcggc caagccaagt tttcacagaa aaagaaagtg
     7681 gcagtaaaag taaatctaaa actcaaaaca gtcgagtttt tcagagtaca actattgatg
     7741 gtgaacgatc gccaatacta ggggccttta aaacgggagc agctattgca accattgacg
     7801 actggtatcc tgaagccact gagccactaa gggtcggacg gtttggggtt catcgcgaag
     7861 atgtcacttg ctaccgtcat ccgtctaccg gaaaagattt tttctcgata ttacaacaag
     7921 cagagcacta tattgaagtg ttgagcgcca acaaaactcc cgctcaagaa actatcaacg
     7981 acatgcactt tttaatggct aacctgatta agggtgggat gttccagcat aaaggagact
     8041 gactgtgaaa tggtattata agacaatcac ctttctgcca gagttgtgca acaacgagtc
     8101 actggctgca aagtgtctcc gcgttctgca tggatttaac tatcagtatg agacacgaaa
     8161 tatcggcgtt tcatttccgc tttggtgtga tgcaacggtt ggaaaaaaga tttcatttgt
     8221 cagcaagaac aagatagaac tcgacttact acttaaacaa cactatttcg tccaaatgga
     8281 acaacttcaa tactttcata tatccaacac tgttctcgtc ccagaagatt gtacatacgt
     8341 ttcctttaga cgctgtcaat ctatagataa gctcacagca gcagggctgg caaggaaaat
     8401 cagacgcctg gagaaacgtg ctctatctag aggcgagcaa tttgacccat catcttttgc
     8461 tcaaaaagag catactgcaa tagcgcacta ccactcactt ggggagtcca gcaaacagac
     8521 gaaccgcaac tttcgactca atatcaggat gctctcggag caaccccgtg aggggaactc
     8581 gatttttagt agctatggct tatcaaattc agaaaactcg tttcagcctg tacccttaat
     8641 ctgaccttta ataaggagat ataccatggc gacaagttta cctacgccct cagcaattac
     8701 gacttcggcg ttagagtatg cattccatac tcccgctcgc aatctaacga aatctcgcgg
     8761 aaaaaacatt catcgttatg tcagtgtaaa gatgagtaag aggattacgg tagaatctac
     8821 tctagagtgt gatgcctgct atcactttga ttttgagcca agtattgttc gcttttgcgc
     8881 tcaaccgatt cgatttttat attatctcaa tggtcagtct cactcctatg ttcctgactt
     8941 tctagttcaa tttgatacca acgagtttgt tctatatgaa gtaaagtcag cttatgctaa
     9001 gaacaaacct gattttgatg ttgaatggga ggcgaaagta aaagcagcaa ctgaactagg
     9061 gctagaattg gagcttgttg aagagagtga tattagggat acggttgtat taaataatct
     9121 taagcgcatg catcgttatg cttcgaaaga tgagctgaat aacgtacata actctctctt
     9181 aaaaataata aagtacaatg gcgcccaatc tgcaagatgc ttgggagaac agttgggttt
     9241 aaaaggccga actgttttac caattttgtg cgatttgctg tcaaggtgtt tactcgatac
     9301 acgtttggat aagcctctat ctcttgaatc tcgatttgag ttggccagtt atggctaaga
     9361 aagggttctc aagtttccat agaaaagcag tctcttccca agatacgctt gaatcgatag
     9421 agcttgtctc tagcgctaat tgtctagaaa gtgttacgta tcaagatata tcagcatttc
     9481 ccgaaacaat tgcggtagag attaatttcc gattaagcat tcttcgcttt ttagcgcgaa
     9541 agtgcgaaac cattgtggcc aaatcaattg aaccacatcg tgtagagcta cagcaaaact
     9601 atagtagaaa aatacccagt gcaataacga tatatcgatg gtggcttgct tttcgaaaat
     9661 cagactacaa ccccattagc ttagcaccta atatcaagga tagaggtaat agagagacaa
     9721 aagtgtcaac agttgttgat tctattatgg aacaggcagt tgaaagagtt atatctggac
     9781 gaaaagtcaa tgttagctct gcatataaac gtgttcgacg aaaagttcgt caatacaatc
     9841 taactcatgg aacgaaatac acgtatccta agtacgaatc tgtaagaaag cgagtaaaaa
     9901 agaaaacccc atttgagtta ttagccgcag ggaaagggga gagagtagct aagagagagt
     9961 ttcgccgaat gggaaaaaag atcctcacgt ctagcgtgct agagagggtt gaaatagatc
    10021 acactgtcgt tgaccttttt gcagtacacg aagagtatcg aatcccattg ggccgacctt
    10081 ggcttactca attggttgat tgttacagta aagctgtaat cggtttttat ttaggtttcg
    10141 agcctcctag ctatgtgtcg gtttcccttg cacttaagaa tgcaatacaa cgcaaagatg
    10201 acttaatctc ctcgtatgaa tcgatcgaga atgaatggct atgttatggc atcccagacc
    10261 tactcgtaac tgataatggt aaagagtttt tgtcgaaagc atttgatcaa gcatgtgaat
    10321 cactattgat caatgtgcat caaaataaag ttgagacgcc cgacaacaaa cctcatgttg
    10381 aacgtaacta cgggactatt aatacttctc tgttagacga tttacctggg aaatccttca
    10441 gccagtacct tcaaagagaa gggtacgact ctgtgggaga agctaccctt acactcaatg
    10501 agattagaga aatttactta atttggttgg tggatattta tcataaaaaa cccaatcaga
    10561 gaggcactaa ttgtcctaat gttgcgtgga aaaagggttg tcaagaatgg gaaccagagg
    10621 agttctctgg ttctaaagac gaattagact ttaaatttgc tattgttgat tacaaacaac
    10681 ttactaaagt agggataact gtctacaaag aactgagtta tagcaatgac cgtttagctg
    10741 aatatagagg gaagaaagga aaccataaag ttcagttcaa gtataaccct gagtgtatgg
    10801 cagttatttg ggtgttggat gaggatatga atgagtactt tacagttaat gcgattgact
    10861 acgaatatgc aagtagagta tcactttggc aacataaata taacatgaaa tatcaagcag
    10921 aactaaattc agcagaatat gatgaggaca aggaaattga tgcagaaata aaaattgaag
    10981 aaatcgcaga tcgttcaatt gttaagacta acaaaatcag agctcggagg cgtggcgcta
    11041 ggcatcaaga gaatagcgca agggctaagt caatcagtaa tgcgaacccg gcctcgatac
    11101 aaaaacatga agatgaaatc gttagtgcag ataatgacga ttgggatatt gattatgtct
    11161 gagaaatcgt caaatgagtg aaacgcgtga ggctcgtata tcaagagcta aaagggcatt
    11221 tgtatccaca ccgagcgtta ggaaaatctt gagttacatg gatagatgta gagatctatc
    11281 agacctagag tctgagccta catgcatgat ggtctatggt gcttcgggtg taggtaaaac
    11341 gaccgtcatc aagaaatact taaatcaaaa cagaagagag tccgaagccg ggggcgatat
    11401 aataccggtt ttgcatattg agttgccaga caatgcgaag ccagtagatg cagcaaggga
    11461 attgctggtt gaaatgggtg acccgctagc actttatgaa actgacttag ctagattgac
    11521 gaaaagactg actgaattaa tccctgcggt cggcgtgaag ctgattatta tcgatgagtt
    11581 ccaacatttg gtggaagaaa ggtcaaatcg ggttcttacc caagtaggta attggctaaa
    11641 aatgatactt aacaaaacga aatgtccaat tgttatattt ggtatgccat actcaaaagt
    11701 tgtactgcaa gcaaactcgc aacttcacgg gcgattttcc attcaggttg aactgcgccc
    11761 ctttagctac cagggaggta gaggtgtatt taaaactttt ttggaatacc ttgataaagc
    11821 cctacctttt gaaaaacagg ctggc
//
