LOCUS       sequence-420646-       14673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis CAB1 promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 14673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pCAB1-YFP-GW
FEATURES             Location/Qualifiers
     source          1..14673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    3500..3554
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(3556..3558,3559..3561,3562..4272)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3601..3622)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3862..3881)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4209..4230
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4342..4466
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4491..4496,4497..4514,4515..4521)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4575..5234
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4641..4660)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5576..5881
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5760..5779
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5922..6046)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6105..6812
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6837..6854)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6837..6853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6846..6868)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7056..7080
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8381..9010
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9439..10512
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10578..10772
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11008..11030)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11116..11256
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11171..11190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11271..11288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11442..12030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11522..11541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13455..14249)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgagact aacttgtgag tgagagtgat taaaactggt tcgatagtgt tggattatat
     1381 agggatattg taacgtttgt tatatctgaa gtaatcttta tctgttcatt cattggattt
     1441 taagattttt gttttatcgt ttagtagtct tacgtggcaa tctctagtcc accagcattt
     1501 gttttgattt tttcattgca gccaagcgtt ttgtgaccac aagttatgag ccactgaata
     1561 cttcaacggt tacacttact tacaccctcg tgacaagaat gatttttgct acaacccaat
     1621 aactaaaact taaagtataa cattttgaaa atatagattc tgatgatcga aacattaata
     1681 taaagtaaca ttataaaaag catttaccca cacataaata tcaatatttt caatggtaat
     1741 tagttcatca tcactaccag tttaatttca attatgaatc gatttcatcg tcactttatc
     1801 caataaccaa aataatctca tgaattatgt gatatcacct taaaactttg acgttttact
     1861 ctttatcgaa gaaacacaac gattaatgaa acactgtttt aacttatcga ccatttctaa
     1921 gaacatgaaa gaactatatt aattcatatg gcatgttcat catcatgggt ttagacttta
     1981 gagaacatat agctactttt tttcttatca tgattcaagt tatctttatt gtaaacttca
     2041 acaacctttt gaacaaactt atttattcgc tttttccgaa ttcatcaaca aattactcct
     2101 caatcacact cctatagaaa acggtttaag ctatcattac atgtctagtt ggttttactc
     2161 agccctagaa gtgttgttta ttgcatcact ttccacgaag cacaattttt cttttttaca
     2221 atcactagac ctcacaggct cacacatatg ctttagagca cattctaaac tttgaactat
     2281 aaaagctgtt aacactaata cactatgcgt tcttttttgc tccaaacact tttgatccat
     2341 tattaggaga cactccactt agaaagattt tctaatcctt tggtcaacta ggaagttcaa
     2401 ggtttttcta aacagaaatt catttcacaa gtaatttaat ttataaggaa atgaatagag
     2461 aaatcaaatc attgaagaac tacaaaatat agattcaagg tcaggtctaa gaaaatattc
     2521 ctgaagctca aaaaagagtt ttcctctcac attatagaat tggcctttac ttcaacattt
     2581 tcccacctat tccacatttg gtcagaacat ttttaattac ttgtggatca atttccggtt
     2641 gaaatgggtt tggtgaatat ccggttcagt tatatggtgg ccgttggaat tggcttatta
     2701 gttgtggccg ttgttgaagc cgttggtatt ggtaagggag aagcagactt gtggctatga
     2761 gtctatgacc atgactcgtg attatggagc tgtcttatga ccctgaccat caccttgatc
     2821 tggtggattc caatgttttc ttcttcttct aataaaatat tatggtcaat acaggtgcta
     2881 attaagatgg taataatttc ttatgtttct gtggtaaagt ttgattcaat tccgtagttt
     2941 tagataatct tatttccata cataaatttt atagttttat ctactttgtt cttatgtttt
     3001 atctctagcc aagagttatt attattatca gaagaagaaa aaaaaaagaa gcatatatac
     3061 aaaaggttta ataaaatgta ttatacaagg caattatcca aatttttttt gttttggttt
     3121 acattgatgc tctcaggatt tcataaggat agagagatct attcgtatac gtgtcacgtc
     3181 atgagtgggt gtttcgccaa tccatgaaac gcacctagat atctaaaaca catatcaatt
     3241 gcgaatctgc gaagtgcgag ccattaacca cgtaagcaaa caaacaatct aaaccccaaa
     3301 aaaaatctat gactagccaa tagcaacctc agagattgat atttcaagat aagacagtat
     3361 ttagatttct gtattatata tagcgaaaat cgcatcaata ccaaaccacc catttcttgg
     3421 cttacaacaa caaatcttaa acgttttact ttgtgctgca ctactcaacc tcctcgagta
     3481 taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     3541 acatttacaa ttaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3601 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3661 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3721 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac
     3781 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3841 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3901 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3961 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4021 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4081 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4141 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     4201 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4261 gagctgtaca agtccggact cagatcacga gctcaagctt caaattctgc agtcgacggt
     4321 accgcgggcc cgggatcatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     4381 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     4441 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
     4501 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     4561 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     4621 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     4681 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     4741 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg
     4801 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     4861 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     4921 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     4981 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     5041 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     5101 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     5161 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     5221 agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt
     5281 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     5341 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     5401 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     5461 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     5521 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     5581 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     5641 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     5701 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     5761 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     5821 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     5881 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     5941 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     6001 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat gatcctaggt
     6061 gagtctagag agttaattaa gacccgggac tagtccctag agtcctgctt taatgagata
     6121 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     6181 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     6241 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
     6301 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
     6361 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     6421 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     6481 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     6541 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     6601 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     6661 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     6721 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     6781 gaattttgcg acaacatgtc gaggctcagc aggacctgca ggcatgcaag cttggcactg
     6841 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6901 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6961 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     7021 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     7081 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     7141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     7201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     7261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     7321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     7381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     7441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     7501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     7561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     7621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     7681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     7741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     7801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     7861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     7921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     7981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     8041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     8101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     8161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     8221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     8281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     8341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     8401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     8461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     8521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     8581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     8641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     8701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     8761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     8821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     8881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     8941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     9001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     9061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     9121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     9181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     9241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     9301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     9361 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
     9421 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     9481 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     9541 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     9601 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     9661 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     9721 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     9781 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     9841 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     9901 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     9961 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
    10021 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
    10081 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
    10141 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
    10201 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
    10261 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
    10321 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
    10381 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
    10441 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
    10501 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
    10561 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
    10621 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
    10681 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
    10741 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
    10801 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
    10861 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
    10921 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
    10981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    11041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    11101 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
    11161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    11221 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
    11281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    11341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    11401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    11461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    11521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    11581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11881 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    12001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    12061 gacgctcagt ggaacgaaaa ctcacgttaa gggctgatga atcccctaat gatttttatc
    12121 aaaatcatta agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa
    12181 atcaataatc agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat
    12241 tctgccccga attacactta aaacgactca acagcttaac gttggcttgc cacgccttac
    12301 ttgactgtaa aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa
    12361 caaaacataa catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc
    12421 gactcgctgt ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg
    12481 tacttgttga ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag
    12541 tcgcactaca cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat
    12601 gttcaaagaa agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca
    12661 ccacaccgct cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga
    12721 agctgggttg gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag
    12781 cggaaaactg gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt
    12841 taggctataa gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat
    12901 ctcgctctaa aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac
    12961 ctaaaatcta ctcagcgtcg gcaaaggagc catggattct agcaactaac ttacctgttg
    13021 aaattcgaac acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa
    13081 ccttccgaga cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca
    13141 gctcagagcg ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc
    13201 ttgcgggcgt tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca
    13261 gaaatcgaaa cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct
    13321 acacaataac aagggaagac ttactcgtgg ctgcaaccct actagctcaa aatttattca
    13381 cacatggtta cgctttgggg aaattatgag gggatctctc agcgttaagg gattttggtc
    13441 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    13501 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    13561 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    13621 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    13681 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    13741 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    13801 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    13861 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    13921 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    13981 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    14041 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    14101 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    14161 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    14221 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
    14281 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
    14341 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    14401 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
    14461 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
    14521 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
    14581 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
    14641 gagctgccgg tcggggagct gttggctggc tgg
//
