LOCUS       sequence-420648-       13612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis ADH1 promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pADH1-YFP-GW
FEATURES             Location/Qualifiers
     source          1..13612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    2439..2493
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(2495..2497,2498..2500,2501..3211)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2540..2561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2801..2820)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3148..3169
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3281..3405
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3430..3435,3436..3453,3454..3460)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3514..4173
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3580..3599)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4515..4820
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4699..4718
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4861..4985)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5044..5751
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5776..5793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5776..5792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5785..5807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5995..6019
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7320..7949
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8378..9451
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9517..9711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9947..9969)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10055..10195
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10110..10129
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10210..10227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10381..10969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10461..10480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12394..13188)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccacactga agaaaaagat tacaccggag aaattaaatg atctatgaat aaagaggtct
     1381 ggaaatagag aaatgtgagc tcttaagagt tgttcttgag gagcttgtga gaaagattaa
     1441 ttaacggtta attacattgt tgaatcaaat tactggtaaa tggttaggtg aataatttaa
     1501 ttttatgtat ttgatatcca acattgatcg acgtacgtac gtagtgaggt agaggcttag
     1561 gttaggaatc aatcgtgaat actatttcaa cgttgattcc cgcgagctga atattttttg
     1621 tgatgtatag taaaacgcat atgattatag gaatcatcat aaagattctt gtcatgaaga
     1681 acaatattaa accaaattat gcattacgag caaaataatt atacaaaaat actgataatt
     1741 ataacttggg tcattgtatt ttctgtgtaa tgtctactat cccttaatta gtcggtttaa
     1801 atcaggaaaa agtataatta atgacactca taattgtatc gttaagactt gaaagtgacg
     1861 gccaagaata caattaagag ccaattagtg atctttcata actttaaaaa tctcacaaaa
     1921 gtagaaaaaa aaaaattcca acttgatgac caagaataat actattaaag agctatttaa
     1981 gatgaaaccg cccgaaacca aaagcattcg atgggtacac cgattactgc ttttagcaac
     2041 accacggcgt gaccatcaag actaattaac taagaccaca ttttaaaaaa actattaata
     2101 attactacaa tttgtaatta aaaagatcaa cgagaaatgc cacgtggacg aatactagca
     2161 acgccaagtg gaaagagcgt tcgagagaac aaggcaaaac caaatacgcc cctagtattc
     2221 tacagatgtc gactggataa ttacaaaaga tttcaataaa cagtactaat taatttctag
     2281 tggtgagttt ttgtaaatat ctacttcttc caattaccag ctgctatata aatccccttc
     2341 tctgtttctc ttttcttaca tcacaatcac acaaaactaa caaaagatca aaagcaagtt
     2401 cttcactgtt gctcgagtat aagagctcta tttttacaac aattaccaac aacaacaaac
     2461 aacaaacaac attacaatta catttacaat taccatggtg agcaagggcg aggagctgtt
     2521 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2581 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2641 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     2701 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2761 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2821 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2881 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2941 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3001 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3061 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     3121 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3181 gatcactctc ggcatggacg agctgtacaa gtccggactc agatcacgag ctcaagcttc
     3241 aaattctgca gtcgacggta ccgcgggccc gggatcatca acaagtttgt acaaaaaagc
     3301 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3361 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3421 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3481 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3541 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3601 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     3661 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     3721 catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     3781 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     3841 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     3901 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3961 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     4021 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     4081 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     4141 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     4201 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     4261 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     4321 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4381 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4441 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4501 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4561 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     4621 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     4681 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     4741 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4801 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     4861 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4921 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4981 gtggttgatg atcctaggtg agtctagaga gttaattaag acccgggact agtccctaga
     5041 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     5101 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     5161 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     5221 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     5281 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     5341 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     5401 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     5461 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     5521 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     5581 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     5641 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     5701 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag
     5761 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5821 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5881 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     5941 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     6001 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     6061 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     6121 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     6181 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     6241 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     6301 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     6361 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     6421 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     6481 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     6541 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     6601 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     6661 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     6721 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     6781 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     6841 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     6901 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     6961 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     7021 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     7081 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     7141 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     7201 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     7261 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     7321 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     7381 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     7441 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     7501 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     7561 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     7621 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     7681 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     7741 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     7801 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     7861 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     7921 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     7981 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     8041 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     8101 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     8161 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     8221 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     8281 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     8341 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     8401 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     8461 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     8521 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     8581 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     8641 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     8701 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     8761 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     8821 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     8881 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     8941 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     9001 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     9061 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     9121 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     9181 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     9241 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     9301 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     9361 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     9421 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     9481 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     9541 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     9601 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     9661 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     9721 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     9781 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     9841 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     9901 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     9961 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10021 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    10081 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10141 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    10201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggctgatgaa
    11041 tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct tgtcatatga
    11101 tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga tgtgcgaact cgatatttta
    11161 cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa cagcttaacg
    11221 ttggcttgcc acgcattact tgactgtaaa actctcactc ttaccgaact tggccgtaac
    11281 ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt gttaggtaat
    11341 cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt tatctgttcg
    11401 ggcaatacga tgcccattgt acttgttgac tggtctgata ttcgtgagca aaaacgactt
    11461 atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta tgagaaagcg
    11521 ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc cgaccttgcg
    11581 agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt taaagtgcca
    11641 tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag aggaaaagta
    11701 caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca tgatatgtca
    11761 tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc aatctcatgc
    11821 caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc gacacggact
    11881 cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc atgggttcta
    11941 gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat ctattcgaag
    12001 cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg actaggccta
    12061 cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat cgccctgatg
    12121 cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg ggacaagcac
    12181 ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt aggcatggaa
    12241 gttttgcggc attctggcta cacaataaca agggaagact tactcgtggc tgcaacccta
    12301 ctagctcaaa atttattcac acatggttac gctttgggga aattatgagg ggatctctca
    12361 gcgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
    12421 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
    12481 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
    12541 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
    12601 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
    12661 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
    12721 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
    12781 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
    12841 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
    12901 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
    12961 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
    13021 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
    13081 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
    13141 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
    13201 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
    13261 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
    13321 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
    13381 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
    13441 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
    13501 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
    13561 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//
