LOCUS       sequence-420653-       14615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis WOL promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pWOL-YFP-GW
FEATURES             Location/Qualifiers
     source          1..14615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    3442..3496
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(3498..3500,3501..3503,3504..4214)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3543..3564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3804..3823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4151..4172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4284..4408
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4433..4438,4439..4456,4457..4463)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4517..5176
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4583..4602)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5518..5823
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5702..5721
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5864..5988)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6047..6754
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6779..6796)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6779..6795)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6788..6810)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6998..7022
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8323..8952
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9381..10454
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10520..10714
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10950..10972)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11058..11198
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11113..11132
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11213..11230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11384..11972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11464..11483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13397..14191)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc ctcacacacc acaccatcat tatctttgga aatttgtaac caactcaaga
     1381 ttttccaaac cgttttatct tcctctacaa aaatccaatt cacgttaaat ctatctcttg
     1441 ctcttgcttc ctccaaaaaa aaaaaaaaat cattcccaga tccatcgata tgaaattgta
     1501 tagaaaaaat ggtattcgat ccaagtttat tgtcttctat ttttcttagg ttaatttcac
     1561 tttattccag attcattgtt tgttttttct ttctcggaag agcacaatgt gagtttcact
     1621 ggcctctgtt ataaacatat atagaaatct gtaacaaaaa tcattactaa aattctgtga
     1681 catgtgcagc gatcaaagaa tcaatagcgg aaaaagaaac tacactgcat tcatctatga
     1741 ctgaaagctt ctgatcaagc catgaaatta aggtatccca aacacgtatc ttctctatgt
     1801 ttatcaatct tgctttaagt tctaattctg catatttcaa aggaaccata caagtgttcc
     1861 taaaatccat ttgaatattc aaaaacttct ctcaaatatc atgtagttat agaagctact
     1921 gtctctaagc gcacgagaga aagctacaca acccacgtca gtttccatct acacatataa
     1981 ggtaataata atattttcat gtatctttaa taatagctct atgttttttt ctgtattttt
     2041 cattataaaa ctcataacta tgttatcatt taatatggta ctaatttaat gggattgatt
     2101 tactattgcc tcaaacatgt aataatttaa tgattttttg tttttaacgt ttttagaaat
     2161 tcatgagcat tttaaatttg tggttaggtc ataacaattt gctattacaa aaaaaagaaa
     2221 cactctaaat aatataaaaa atagtttacc gtataatact agtagtaaat aaataatttg
     2281 attgttattc ataaattttg aattctaaaa tctcctgaat caactcatgc aattgtctta
     2341 agaattacac gtggataaat catgggctta tgagtcaggc ccatttaacc ggggtatttt
     2401 cgtagttaag agactagaat ggtgggtatt tcaggtaaaa ggtctatggg gccagatctg
     2461 cgctttgtcg cgatgtcatt atcgccaaag atatgcgata gcgactctcg tacaaagtct
     2521 ctcactcacc tatatttttt gttttcttat atttcaacaa aaaaacgttt tattttcctt
     2581 ttggtgtaag taaaaaaaca aaacaaaacg ttttatttct aaagttcaga aaacttattt
     2641 ataccaagga aaaaatagat aataaatttt gagaagttgg tgactatata ttacttcact
     2701 tattcaagaa atttaaacat ggtaaatgtt actttaaatg ttaaatgatg tataagaaat
     2761 gtaatgaaat tgaataaatg tagttttaaa gatgttttaa ttagtaagac aaacctagtt
     2821 agtgtcacaa taattatatt tttttttttg tcatccaaaa ttattaaagc tcaagtaaac
     2881 caatcctgag ggatattatt tacaaatgtg atatgatgcg gttcggtgcg gatcttccgc
     2941 gccaaattat acgcttttat attagcatta taaaaaatta tagataaaga gaagtttgtg
     3001 aattcttcat tgtcgctttg caatttctct aaatacacag taaataccga caattcggtt
     3061 agagaaaata tatctatttc gtataataat gttaactttg aggagatttt gggtaaaata
     3121 ataacttttg ttggatggat catatcatga gccattaaga aaaagtccaa aacttttctt
     3181 cttcaaagtt ggactcaagt tagaaaaaga aaaaagagct agagagatat aaaaatgaaa
     3241 agaaagttca tggcaaaaaa ctgatataga cagagacaca gagagagaga aacgtatctg
     3301 aagaaaatct aaaaaattcg attcaatttt tttcttactt ttaaaagcaa aaaatctcac
     3361 taaaacaaaa gaagaagaaa gaagaaagaa aatggaatac ctacatttga agtgctcgag
     3421 tataagagct ctatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     3481 ttacatttac aattaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3661 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct
     3721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3961 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4081 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     4141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4201 acgagctgta caagtccgga ctcagatcac gagctcaagc ttcaaattct gcagtcgacg
     4261 gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     4321 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     4381 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     4441 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     4501 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     4561 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     4621 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     4681 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg agttccgtat
     4741 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4801 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     4861 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     4921 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     4981 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     5041 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     5101 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     5161 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
     5221 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     5281 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     5341 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     5401 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     5461 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     5521 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     5581 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     5641 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     5701 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     5761 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     5821 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     5881 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     5941 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atgatcctag
     6001 gtgagtctag agagttaatt aagacccggg actagtccct agagtcctgc tttaatgaga
     6061 tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa
     6121 aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat
     6181 atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac
     6241 cctactactt atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat
     6301 agcgacatct atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc
     6361 aattttaaaa aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt
     6421 caaatttcaa aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa
     6481 cgctcactga agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt
     6541 gagtattggc cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg
     6601 cggccgggta accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg
     6661 ccccaaatga agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg
     6721 gcgaattttg cgacaacatg tcgaggctca gcaggacctg caggcatgca agcttggcac
     6781 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6841 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6901 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
     6961 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     7021 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     7081 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     7141 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     7201 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     7261 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     7321 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     7381 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     7441 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     7501 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     7561 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     7621 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     7681 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     7741 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     7801 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     7861 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     7921 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     7981 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     8041 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     8101 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     8161 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     8221 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     8281 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     8341 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     8401 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     8461 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     8521 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     8581 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     8641 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     8701 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     8761 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     8821 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     8881 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     8941 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     9001 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     9061 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     9121 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     9181 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     9241 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     9301 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
     9361 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     9421 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     9481 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     9541 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     9601 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     9661 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     9721 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     9781 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     9841 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     9901 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     9961 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
    10021 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
    10081 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
    10141 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
    10201 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
    10261 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
    10321 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
    10381 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
    10441 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
    10501 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
    10561 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
    10621 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
    10681 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
    10741 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
    10801 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
    10861 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
    10921 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10981 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    11041 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
    11101 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11161 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    11221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    11341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11821 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    12001 ctgacgctca gtggaacgaa aactcacgtt aagggctgat gaatccccta atgattttta
    12061 tcaaaatcat taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa
    12121 aaatcaataa tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca
    12181 attctgcccc gaattacact taaaacgact caacagctta acgttggctt gccacgcctt
    12241 acttgactgt aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag
    12301 aacaaaacat aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga
    12361 gcgactcgct gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat
    12421 tgtacttgtt gactggtctg atatccgtga gcaaaaacgg cttatggtat tgcgagcttc
    12481 agtcgcacta cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca
    12541 atgttcaaag aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa
    12601 caccacaccg ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga
    12661 gaagctgggt tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg
    12721 agcggaaaac tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac
    12781 tttaggctat aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa
    12841 atctcgctct aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc
    12901 acctaaaatc tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt
    12961 tgaaattcga acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga
    13021 aaccttccga gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag
    13081 cagctcagag cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg
    13141 gcttgcgggc gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt
    13201 cagaaatcga aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg
    13261 ctacacaata acaagggaag acttactcgt ggctgcaacc ctactagctc aaaatttatt
    13321 cacacatggt tacgctttgg ggaaattatg aggggatctc tcagcgttaa gggattttgg
    13381 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    13441 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    13501 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    13561 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    13621 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    13681 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    13741 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    13801 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    13861 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    13921 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    13981 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    14041 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    14101 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    14161 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    14221 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    14281 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    14341 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    14401 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    14461 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    14521 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    14581 ccgagctgcc ggtcggggag ctgttggctg gctgg
//
