LOCUS       sequence-420655-       14685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis SCR promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 14685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pSCR-YFP-GW
FEATURES             Location/Qualifiers
     source          1..14685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    3512..3566
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(3568..3570,3571..3573,3574..4284)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3613..3634)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3874..3893)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4221..4242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4354..4478
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4503..4508,4509..4526,4527..4533)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4587..5246
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4653..4672)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5588..5893
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5772..5791
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5934..6058)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6117..6824
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6849..6866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6849..6865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6858..6880)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7068..7092
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8393..9022
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9451..10524
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10590..10784
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11020..11042)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11128..11268
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11183..11202
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11283..11300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11454..12042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11534..11553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13467..14261)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgattgtga tcctctgcaa caaagcggat tttgctggtg ttgaatggat aagggataga
     1381 ggaagaggac tttgtttatc agaaaccttt tgatgggcct taatgggcct ataaactgta
     1441 actctgtagc gctttgccaa caagagactt tttaaggttt ttgttgccaa acagatattt
     1501 gcatttgggc tatgtaatgt tagaattatt ttataatgta tgctattgct agatattgtt
     1561 taagtgcatt tgtgatttac aaacatttca tttttatttt ggttttaatg agcatttcta
     1621 ttatagagac tttgatgtta ataaatggtg ttctaagata tattaaaata ttttatatac
     1681 tttcttaaaa ttggataaat tttgggaaaa tccttaatat cagttaaatt gaagataaag
     1741 agtattaaaa aaaactatgt agtaaaatac atttcacatt ttttgtgtat aatagtacat
     1801 ggtattcgtt aagatcactc aaaaattaac aaattaagtc taaaagggca gaaaagacta
     1861 ttcaaatatg gacttggaga aagacattca gctttttacg ctgagaaact ttcatattga
     1921 gccgtgtgtt tgtgttgtga agagaagtaa taaaaaataa tttgaagtga aaaaggagaa
     1981 gaaaaaataa gatcgtagaa agcgtggatg gtttcttctt gggttcactg ccatgcgatt
     2041 attaaattgg ccatggggct agtgtttgac gtacaaaagt ctaaaaattg tcagtcaaac
     2101 aggtccaaaa ctttgtaaga aaaataatat aataatagca aattttctaa aaattgttaa
     2161 aaaaagaaca aaagggaaaa gatgaggatg cagatgaaag caaaatgtca aacactagtt
     2221 tcagatttta tcgggaactg gggtttgaca gttggtgtat gtatgtaatg gcctctcatc
     2281 aaaacatgtg catctttttc cttttttgtt atttactgtt ttagctctac gtcttgtcca
     2341 attcctctca agtaaaatgc ctttaatatg atactaatat acaaggggac taatgctttt
     2401 tcccttttct tatccttgtt ttgtctaaat ctttacttgg attcctttat ttttctcctc
     2461 tctttagatt agtacggttt aaggaatacc atctttctaa ttttagcaca aaattgcaag
     2521 ttggtgcccc atcttagtaa gcacatcgta ccacactttg attgtgtgag agacttcttc
     2581 atcccatctc tcataccaaa cctaaatcaa atgactagtg gtgcaacctg ctgactccat
     2641 atgaccataa ctaataaatc ggtttatgaa tccaactcat gtagctctat agaatagaaa
     2701 cccattcatt tcacataatg aactgaatct gacattttat ttacatcatt tactactcaa
     2761 ttttgtaatt agcaagatca tctttttcat tattcaacaa ttttgatatt ccataattta
     2821 ttaactttgt catacatcat aatattctga aattttgtta tatattgtac cggttccacg
     2881 aaatagagct ctattattat agaccaaaca aacaaaatat tatcttcttg tggttagttc
     2941 gagagagagg tcaagaagaa acgaaatgga tcggcaaacg gaagacgtca aacacacaac
     3001 gacgaacatt ttccgatcac ccacctaatc tcttcccatt tttattattt ttcaaaactc
     3061 aaattaatta agaagaaaaa aacagaaaca gagagagaaa gagttaagat gaatagagat
     3121 agaaagagtc attaaatgta cgaagcgaca ttcacaataa ttcgaaaggt ggaagacgac
     3181 ttagatacgg ccaggcttca ctgtcctcct cgtcctcctc aattacccct aacccctttt
     3241 tccgggattc atctccaacc cacatccttc caaattctca ccccctcact gagtttttgc
     3301 tttttctcct catcggagat cgtgaagacg atcaagtaat ttaagaatcc caccattgat
     3361 aaaagagtct agcttttcta ctaccaaacc tttttctgtt tggaaatttt cgattttgga
     3421 tttaaccctt ttcttacctt atttataacc atgcaatctc acgaccaaca acccttcaat
     3481 ctccctcgag tataagagct ctatttttac aacaattacc aacaacaaca aacaacaaac
     3541 aacattacaa ttacatttac aattaccatg gtgagcaagg gcgaggagct gttcaccggg
     3601 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3661 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3721 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     3781 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3841 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3901 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3961 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4021 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4081 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4141 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     4201 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4261 ctcggcatgg acgagctgta caagtccgga ctcagatcac gagctcaagc ttcaaattct
     4321 gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa agctgaacga
     4381 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     4441 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     4501 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     4561 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     4621 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     4681 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     4741 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     4801 agttccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     4861 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     4921 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     4981 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     5041 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     5101 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     5161 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     5221 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
     5281 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     5341 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     5401 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     5461 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     5521 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     5581 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     5641 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     5701 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     5761 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     5821 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     5881 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     5941 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     6001 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
     6061 atgatcctag gtgagtctag agagttaatt aagacccggg actagtccct agagtcctgc
     6121 tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc
     6181 acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc
     6241 taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta
     6301 ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat attgtgctga
     6361 ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg cgttttatta
     6421 ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga ctgattacat
     6481 aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac accgagcggc
     6541 gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat gggtgagatt
     6601 ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca ttcaacccgg
     6661 tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc atttttttgg
     6721 tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca aatcgttggg
     6781 cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg caggcatgca
     6841 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     7021 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     7081 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     7141 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     7201 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     7261 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     7321 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     7381 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     7441 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     7501 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     7561 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     7621 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     7681 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     7741 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     7801 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     7861 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     7921 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     7981 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     8041 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     8101 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     8161 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     8221 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     8281 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     8341 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     8401 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     8461 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     8521 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     8581 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     8641 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     8701 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     8761 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     8821 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     8881 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     8941 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     9001 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     9061 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     9121 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     9181 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     9241 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     9301 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     9361 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
     9421 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     9481 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     9541 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     9601 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     9661 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     9721 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     9781 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     9841 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     9901 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     9961 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    10021 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    10081 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    10141 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    10201 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    10261 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    10321 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    10381 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    10441 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    10501 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    10561 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
    10621 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    10681 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    10741 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    10801 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    10861 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    10921 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    10981 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    11041 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11101 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    11161 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    11221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    11281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11881 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggctgat gaatccccta
    12121 atgattttta tcaaaatcat taagttaagg tagatacaca tcttgtcata tgatcaaatg
    12181 gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga actcgatatt ttacacgact
    12241 ctctttacca attctgcccc gaattacact taaaacgact caacagctta acgttggctt
    12301 gccacgcctt acttgactgt aaaactctca ctcttaccga acttggccgt aacctgccaa
    12361 ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg attgttaggt aatcgtcacc
    12421 tccacaaaga gcgactcgct gtataccgtt ggcatgctag ctttatctgt tcgggcaata
    12481 cgatgcccat tgtacttgtt gactggtctg atatccgtga gcaaaaacgg cttatggtat
    12541 tgcgagcttc agtcgcacta cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc
    12601 tttcagagca atgttcaaag aaagctcatg accaatttct agccgacctt gcgagcattc
    12661 taccgagtaa caccacaccg ctcattgtca gtgatgctgg ctttaaagtg ccatggtata
    12721 aatccgttga gaagctgggt tggtactggt taagtcgagt aagaggaaaa gtacaatatg
    12781 cagacctagg agcggaaaac tggaaaccta tcagcaactt acatgatatg tcatctagtc
    12841 actcaaagac tttaggctat aagaggctga ctaaaagcaa tccaatctca tgccaaattc
    12901 tattgtataa atctcgctct aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc
    12961 accacccgtc acctaaaatc tactcagcgt cggcaaagga gccatggatt ctagcaacta
    13021 acttacctgt tgaaattcga acacccaaac aacttgttaa tatctattcg aagcgaatgc
    13081 agattgaaga aaccttccga gacttgaaaa gtcctgccta cggactaggc ctacgccata
    13141 gccgaacgag cagctcagag cgttttgata tcatgctgct aatcgccctg atgcttcaac
    13201 taacatgttg gcttgcgggc gttcatgctc agaaacaagg ttgggacaag cacttccagg
    13261 ctaacacagt cagaaatcga aacgtactct caacagttcg cttaggcatg gaagttttgc
    13321 ggcattctgg ctacacaata acaagggaag acttactcgt ggctgcaacc ctactagctc
    13381 aaaatttatt cacacatggt tacgctttgg ggaaattatg aggggatctc tcagcgttaa
    13441 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
    13501 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
    13561 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
    13621 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
    13681 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
    13741 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
    13801 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
    13861 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
    13921 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
    13981 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
    14041 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
    14101 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
    14161 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
    14221 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
    14281 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    14341 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    14401 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
    14461 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    14521 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
    14581 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
    14641 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctgg
//
