LOCUS       sequence-420662-       13252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis COBL promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pCOBL-YFP-GW
FEATURES             Location/Qualifiers
     source          1..13252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    2079..2133
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(2135..2137,2138..2140,2141..2851)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2180..2201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2441..2460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2788..2809
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2921..3045
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3070..3075,3076..3093,3094..3100)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3154..3813
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3220..3239)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4155..4460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4339..4358
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4501..4625)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4684..5391
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5416..5433)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5416..5432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5447)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5635..5659
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6960..7589
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8018..9091
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9157..9351
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9587..9609)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9695..9835
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9750..9769
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9850..9867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10021..10609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10101..10120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12034..12828)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgactca tgtttggttg tactactgtc aatttggtag gattgtgaga ttttgcaaga
     1381 ctgtttattc ctgttgtttt gataaaactt ttacttggtc acctgcaatg caagactagc
     1441 aaagaatact ataaattttg ttctgttttc ttgtatggga cgggcttaaa cgggcctaac
     1501 agtcgttctt tatcggccca ctttaacaac acctaattgt ctagtctagt agtagtattc
     1561 tctagagact ttcaaaaagt ctttggatta ctaaatagta aatgcgaata taagtaaact
     1621 ataaatgtga ataagaatgt ttgaaattga aaaaaagaat tgctagctaa gtgcattaat
     1681 gcttgtgcat taagtcaaat tataacactt ggcacgaaaa aacccatctc atcccccact
     1741 aaagcagcct tcttttggct ttcattaatc tctctgcaca caaaaaccca aaaagctgga
     1801 tgtctccatt ttcaatgaac aattatgagt tcttttttta aaaactcatt atactttgaa
     1861 gattaatcac aaagatttcg aagctaattc gtcatttctt gttactataa tcttttcatt
     1921 gttattaatc actttggctt aagtttctgt atattacgtc gagcttcttc agtcttcatc
     1981 cttgtcttgt cacttgtcag agacagaaga ttaaagaaga agaggcgttt ctctctcttt
     2041 ttttgcttca gctcgagtat aagagctcta tttttacaac aattaccaac aacaacaaac
     2101 aacaaacaac attacaatta catttacaat taccatggtg agcaagggcg aggagctgtt
     2161 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2221 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2281 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     2341 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2401 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2461 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2521 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2581 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2641 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2701 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     2761 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2821 gatcactctc ggcatggacg agctgtacaa gtccggactc agatcacgag ctcaagcttc
     2881 aaattctgca gtcgacggta ccgcgggccc gggatcatca acaagtttgt acaaaaaagc
     2941 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3001 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3061 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3121 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     3301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     3361 catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     3421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     3481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     3541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     3661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     3721 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     3781 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     3841 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3901 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     3961 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4021 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4081 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4141 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4201 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     4261 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     4321 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     4381 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4441 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     4501 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4561 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4621 gtggttgatg atcctaggtg agtctagaga gttaattaag acccgggact agtccctaga
     4681 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     4741 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     4801 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     4861 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     4921 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     4981 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     5041 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     5101 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     5161 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     5221 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     5281 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     5341 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag
     5401 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5461 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5521 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     5581 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     5641 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     5701 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     5761 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     5821 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     5881 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     5941 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     6001 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     6061 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     6121 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     6181 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     6241 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     6301 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     6361 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     6421 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     6481 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     6541 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     6601 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     6661 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     6721 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     6781 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     6841 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     6901 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     6961 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     7021 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     7081 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     7141 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     7201 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     7261 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     7321 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     7381 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     7441 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     7501 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     7561 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     7621 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     7681 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     7741 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     7801 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     7861 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     7921 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     7981 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     8041 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     8101 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     8161 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     8221 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     8281 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     8341 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     8401 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     8461 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     8521 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     8581 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     8641 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     8701 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     8761 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     8821 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     8881 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     8941 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     9001 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     9061 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     9121 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     9181 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     9241 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     9301 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     9361 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     9421 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     9481 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     9541 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     9601 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9661 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     9721 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9781 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     9841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10441 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggctgatgaa
    10681 tcccctaatg atttttatca aaatcattaa gttaaggtag atacacatct tgtcatatga
    10741 tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa tgtgcgaact cgatatttta
    10801 cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa cagcttaacg
    10861 ttggcttgcc acgccttact tgactgtaaa actctcactc ttaccgaact tggccgtaac
    10921 ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt gttaggtaat
    10981 cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt tatctgttcg
    11041 ggcaatacga tgcccattgt acttgttgac tggtctgata tccgtgagca aaaacggctt
    11101 atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta tgagaaagcg
    11161 ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc cgaccttgcg
    11221 agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt taaagtgcca
    11281 tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag aggaaaagta
    11341 caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca tgatatgtca
    11401 tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc aatctcatgc
    11461 caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc gacacggact
    11521 cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc atggattcta
    11581 gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat ctattcgaag
    11641 cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg actaggccta
    11701 cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat cgccctgatg
    11761 cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg ggacaagcac
    11821 ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt aggcatggaa
    11881 gttttgcggc attctggcta cacaataaca agggaagact tactcgtggc tgcaacccta
    11941 ctagctcaaa atttattcac acatggttac gctttgggga aattatgagg ggatctctca
    12001 gcgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
    12061 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
    12121 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
    12181 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
    12241 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
    12301 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
    12361 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
    12421 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
    12481 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
    12541 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
    12601 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
    12661 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
    12721 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
    12781 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
    12841 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
    12901 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
    12961 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
    13021 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
    13081 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
    13141 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
    13201 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//
