LOCUS       sequence-420663-       13832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. Arabidopsis LBD16 promoter driving expression of 
            N-terminal YFP fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pLBD16-YFP-GW
FEATURES             Location/Qualifiers
     source          1..13832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    2659..2713
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(2715..2717,2718..2720,2721..3431)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2760..2781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3021..3040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3368..3389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3501..3625
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3650..3655,3656..3673,3674..3680)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3734..4393
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3800..3819)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4735..5040
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4919..4938
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5081..5205)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5264..5971
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5996..6013)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5996..6012)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6005..6027)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6215..6239
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7540..8169
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8598..9671
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9737..9931
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10167..10189)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10275..10415
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10330..10349
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10430..10447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10601..11189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10681..10700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12614..13408)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcggaaga acttataaaa taacttttct aaaattaaaa taaaaataaa gaatgaatga
     1381 aagaagatag acaaaattga ttattaattg agtttgcttt gaagctccaa gaaccaaacg
     1441 tatccgggta taaaataata cgagataaag tgatagaaga cctaagacta cctcattact
     1501 aaaaatatgg atatttttct atacaaatcc acgaatgaaa agaaaaagaa gttggactaa
     1561 ttaaattgaa atccaataat tgtaaatata atattaaaac ccaataaatt agaagtctca
     1621 tgttgcagtc tccattattg attgtctcct tagaatccaa catgtgaccc tccattgtct
     1681 accattcaca ccctttggct cactcgataa atttggacat taattgcatt cattttattt
     1741 gtaagaacca gaatctcagt agatgttatt tgtgttgagt aaactgttgt tatttataga
     1801 ctaggctatt cttcgtatac actttgatac tgatttttag ttcatcatct atctaccgac
     1861 taaatatagt gtttctatgt ggcttctcct caaatgtcaa atttatttct ttaatgttgt
     1921 ttgagtttca aacttgcaaa gtgcaaacca attttggttg ttatactaaa accacacata
     1981 taattaaaga cacccactat agcataaaat tcgtcgtgta agcaatttta ctatagtcta
     2041 aactccataa aaggacgtcg aaaaatattg tgatattgga attatagtgt cagtttgttt
     2101 ttctttgggc aaatggattg tagtttcatt atatattaca atttataaac gaaacgataa
     2161 gtatggctca tactttagtg atggagaatt aaagtatttt atattcagag atgacacaat
     2221 atggtagacc aaaaataact aattaaaata gtatcatatt agatatttat atgtgattgt
     2281 aaatttgatc attgagtgaa cattttaaat tagtatgccc aagaaagaaa agaaaaaaaa
     2341 aaaactcttt ctctctcatg ataatgttcc ttttataaaa tccacccaaa gacaccaaac
     2401 acttctccat catcatttca tttcttgttc tttttccacc atcaccattt cctagttcgc
     2461 tcgtgcacat ctccgacgat cccaattcta aaatcatcac ttcttccaat cttttcaaca
     2521 tctcttcaaa atatccagtc aacaaaaaaa aaagtactta aaacaaggaa accccttctt
     2581 tctttttgta catataacat aaaaatcatc acttttttgt tcgtttcgcc gctcgagtat
     2641 aagagctcta tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     2701 catttacaat taccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2761 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2821 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2881 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
     2941 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3001 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3061 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3121 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     3181 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     3241 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3301 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     3361 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3421 agctgtacaa gtccggactc agatcacgag ctcaagcttc aaattctgca gtcgacggta
     3481 ccgcgggccc gggatcatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3541 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3601 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     3661 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     3721 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     3781 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     3841 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     3901 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc
     3961 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     4021 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     4081 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     4141 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     4201 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     4261 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     4321 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     4381 gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt
     4441 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     4501 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     4561 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     4621 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     4681 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     4741 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     4801 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     4861 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     4921 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     4981 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     5041 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     5101 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     5161 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgatg atcctaggtg
     5221 agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt aatgagatat
     5281 gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa
     5341 cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc
     5401 acccgttact atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct
     5461 actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc
     5521 gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat
     5581 tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa
     5641 atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc
     5701 tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag
     5761 tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg
     5821 ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc
     5881 caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg
     5941 aattttgcga caacatgtcg aggctcagca ggacctgcag gcatgcaagc ttggcactgg
     6001 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6061 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6121 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     6181 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     6241 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     6301 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     6361 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     6421 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     6481 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     6541 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     6601 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     6661 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     6721 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     6781 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     6841 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     6901 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     6961 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     7021 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     7081 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     7141 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     7201 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     7261 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     7321 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     7381 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     7441 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     7501 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     7561 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     7621 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     7681 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     7741 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     7801 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     7861 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     7921 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     7981 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     8041 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     8101 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     8161 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     8221 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     8281 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     8341 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     8401 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     8461 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     8521 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
     8581 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     8641 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     8701 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     8761 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     8821 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     8881 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     8941 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     9001 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     9061 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     9121 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     9181 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     9241 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     9301 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     9361 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     9421 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     9481 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     9541 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     9601 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     9661 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     9721 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     9781 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     9841 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     9901 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     9961 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
    10021 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
    10081 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
    10141 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    10201 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10261 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    10321 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    10381 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    10441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11041 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11221 acgctcagtg gaacgaaaac tcacgttaag ggctgatgaa tcccctaatg atttttatca
    11281 aaatcattaa gttaaggtag atacacatct tgtcatatga tcaaatggtt tcgccaaaaa
    11341 tcaataatca gacaacaaaa tgtgcgaact cgatatttta cacgactctc tttaccaatt
    11401 ctgccccgaa ttacacttaa aacgactcaa cagcttaacg ttggcttgcc acgccttact
    11461 tgactgtaaa actctcactc ttaccgaact tggccgtaac ctgccaacca aagcgagaac
    11521 aaaacataac atcaaacgaa tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg
    11581 actcgctgta taccgttggc atgctagctt tatctgttcg ggcaatacga tgcccattgt
    11641 acttgttgac tggtctgata tccgtgagca aaaacggctt atggtattgc gagcttcagt
    11701 cgcactacac ggtcgttctg ttactcttta tgagaaagcg ttcccgcttt cagagcaatg
    11761 ttcaaagaaa gctcatgacc aatttctagc cgaccttgcg agcattctac cgagtaacac
    11821 cacaccgctc attgtcagtg atgctggctt taaagtgcca tggtataaat ccgttgagaa
    11881 gctgggttgg tactggttaa gtcgagtaag aggaaaagta caatatgcag acctaggagc
    11941 ggaaaactgg aaacctatca gcaacttaca tgatatgtca tctagtcact caaagacttt
    12001 aggctataag aggctgacta aaagcaatcc aatctcatgc caaattctat tgtataaatc
    12061 tcgctctaaa ggccgaaaaa atcagcgctc gacacggact cattgtcacc acccgtcacc
    12121 taaaatctac tcagcgtcgg caaaggagcc atggattcta gcaactaact tacctgttga
    12181 aattcgaaca cccaaacaac ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac
    12241 cttccgagac ttgaaaagtc ctgcctacgg actaggccta cgccatagcc gaacgagcag
    12301 ctcagagcgt tttgatatca tgctgctaat cgccctgatg cttcaactaa catgttggct
    12361 tgcgggcgtt catgctcaga aacaaggttg ggacaagcac ttccaggcta acacagtcag
    12421 aaatcgaaac gtactctcaa cagttcgctt aggcatggaa gttttgcggc attctggcta
    12481 cacaataaca agggaagact tactcgtggc tgcaacccta ctagctcaaa atttattcac
    12541 acatggttac gctttgggga aattatgagg ggatctctca gcgttaaggg attttggtca
    12601 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
    12661 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
    12721 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
    12781 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
    12841 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
    12901 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
    12961 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
    13021 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
    13081 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
    13141 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
    13201 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    13261 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    13321 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    13381 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    13441 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    13501 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    13561 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    13621 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    13681 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
    13741 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
    13801 agctgccggt cggggagctg ttggctggct gg
//
