LOCUS       sequence-420664-       12469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by CAB1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pCAB1-GW
FEATURES             Location/Qualifiers
     source          1..12469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3482..3606
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3631..3636,3637..3654,3655..3661)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3715..4374
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3781..3800)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4716..5021
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4900..4919
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5062..5186)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5239..5946
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5971..5988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5971..5987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5980..6002)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6190..6214
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7515..8144
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8573..9646
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9712..9906
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10142..10164)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10250..10390
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10305..10324
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10405..10422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10576..11164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10656..10675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11251..12045)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgagact aacttgtgag tgagagtgat taaaactggt tcgatagtgt tggattatat
     1381 agggatattg taacgtttgt tatatctgaa gtaatcttta tctgttcatt cattggattt
     1441 taagattttt gttttatcgt ttagtagtct tacgtggcaa tctctagtcc accagcattt
     1501 gttttgattt tttcattgca gccaagcgtt ttgtgaccac aagttatgag ccactgaata
     1561 cttcaacggt tacacttact tacaccctcg tgacaagaat gatttttgct acaacccaat
     1621 aactaaaact taaagtataa cattttgaaa atatagattc tgatgatcga aacattaata
     1681 taaagtaaca ttataaaaag catttaccca cacataaata tcaatatttt caatggtaat
     1741 tagttcatca tcactaccag tttaatttca attatgaatc gatttcatcg tcactttatc
     1801 caataaccaa aataatctca tgaattatgt gatatcacct taaaactttg acgttttact
     1861 ctttatcgaa gaaacacaac gattaatgaa acactgtttt aacttatcga ccatttctaa
     1921 gaacatgaaa gaactatatt aattcatatg gcatgttcat catcatgggt ttagacttta
     1981 gagaacatat agctactttt tttcttatca tgattcaagt tatctttatt gtaaacttca
     2041 acaacctttt gaacaaactt atttattcgc tttttccgaa ttcatcaaca aattactcct
     2101 caatcacact cctatagaaa acggtttaag ctatcattac atgtctagtt ggttttactc
     2161 agccctagaa gtgttgttta ttgcatcact ttccacgaag cacaattttt cttttttaca
     2221 atcactagac ctcacaggct cacacatatg ctttagagca cattctaaac tttgaactat
     2281 aaaagctgtt aacactaata cactatgcgt tcttttttgc tccaaacact tttgatccat
     2341 tattaggaga cactccactt agaaagattt tctaatcctt tggtcaacta ggaagttcaa
     2401 ggtttttcta aacagaaatt catttcacaa gtaatttaat ttataaggaa atgaatagag
     2461 aaatcaaatc attgaagaac tacaaaatat agattcaagg tcaggtctaa gaaaatattc
     2521 ctgaagctca aaaaagagtt ttcctctcac attatagaat tggcctttac ttcaacattt
     2581 tcccacctat tccacatttg gtcagaacat ttttaattac ttgtggatca atttccggtt
     2641 gaaatgggtt tggtgaatat ccggttcagt tatatggtgg ccgttggaat tggcttatta
     2701 gttgtggccg ttgttgaagc cgttggtatt ggtaagggag aagcagactt gtggctatga
     2761 gtctatgacc atgactcgtg attatggagc tgtcttatga ccctgaccat caccttgatc
     2821 tggtggattc caatgttttc ttcttcttct aataaaatat tatggtcaat acaggtgcta
     2881 attaagatgg taataatttc ttatgtttct gtggtaaagt ttgattcaat tccgtagttt
     2941 tagataatct tatttccata cataaatttt atagttttat ctactttgtt cttatgtttt
     3001 atctctagcc aagagttatt attattatca gaagaagaaa aaaaaaagaa gcatatatac
     3061 aaaaggttta ataaaatgta ttatacaagg caattatcca aatttttttt gttttggttt
     3121 acattgatgc tctcaggatt tcataaggat agagagatct attcgtatac gtgtcacgtc
     3181 atgagtgggt gtttcgccaa tccatgaaac gcacctagat atctaaaaca catatcaatt
     3241 gcgaatctgc gaagtgcgag ccattaacca cgtaagcaaa caaacaatct aaaccccaaa
     3301 aaaaatctat gactagccaa tagcaacctc agagattgat atttcaagat aagacagtat
     3361 ttagatttct gtattatata tagcgaaaat cgcatcaata ccaaaccacc catttcttgg
     3421 cttacaacaa caaatcttaa acgttttact ttgtgctgca ctactcaacc tcctcgagat
     3481 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     3541 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     3601 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     3661 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     3721 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     3781 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     3841 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     3901 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg
     3961 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     4021 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     4081 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     4141 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     4201 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     4261 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     4321 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct
     4381 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     4441 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     4501 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     4561 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     4621 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     4681 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     4741 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     4801 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     4861 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     4921 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     4981 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     5041 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     5101 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     5161 cgttcagctt tcttgtacaa agtggtgcct aggtgagtct agagagttaa ttaagacccg
     5221 ggactagtcc ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata
     5281 tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct
     5341 taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga
     5401 ataatattct ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa
     5461 tgaaaacaat atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat
     5521 aacaaacaat tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc
     5581 aaacctaaaa gactgattac ataaatctta ttcaaatttc aaaagtgccc caggggctag
     5641 tatctacgac acaccgagcg gcgaactaat aacgctcact gaagggaact ccggttcccc
     5701 gccggcgcgc atgggtgaga ttccttgaag ttgagtattg gccgtccgct ctaccgaaag
     5761 ttacgggcac cattcaaccc ggtccagcac ggcggccggg taaccgactt gctgccccga
     5821 gaattatgca gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg
     5881 acagtgacga caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct
     5941 cagcaggacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
     6001 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6061 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6121 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     6181 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     6241 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     6301 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     6361 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     6421 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     6481 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     6541 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     6601 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     6661 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     6721 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     6781 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     6841 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     6901 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     6961 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     7021 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     7081 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     7141 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     7201 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     7261 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     7321 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     7381 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     7441 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     7501 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     7561 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     7621 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     7681 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     7741 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     7801 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     7861 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     7921 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     7981 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     8041 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     8101 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     8161 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     8221 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     8281 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     8341 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     8401 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     8461 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     8521 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     8581 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     8641 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     8701 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     8761 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     8821 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     8881 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     8941 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     9001 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     9061 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     9121 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     9181 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     9241 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     9301 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     9361 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     9421 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     9481 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     9541 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     9601 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     9661 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     9721 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     9781 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     9841 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     9901 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     9961 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
    10021 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
    10081 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
    10141 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10201 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
    10261 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
    10321 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10381 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10981 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11221 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    11281 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    11341 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    11401 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    11461 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    11521 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    11581 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    11641 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    11701 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    11761 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    11821 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    11881 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    11941 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    12001 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    12061 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    12121 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    12181 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    12241 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    12301 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    12361 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    12421 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
//
