LOCUS       sequence-420665-       11408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by ADH1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pADH1-GW
FEATURES             Location/Qualifiers
     source          1..11408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    2421..2545
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2570..2575,2576..2593,2594..2600)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2654..3313
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2720..2739)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3655..3960
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3839..3858
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4001..4125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4178..4885
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4910..4927)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4910..4926)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4919..4941)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5129..5153
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6454..7083
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7512..8585
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8651..8845
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9081..9103)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9189..9329
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9244..9263
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9344..9361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9515..10103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9595..9614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10190..10984)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccacactga agaaaaagat tacaccggag aaattaaatg atctatgaat aaagaggtct
     1381 ggaaatagag aaatgtgagc tcttaagagt tgttcttgag gagcttgtga gaaagattaa
     1441 ttaacggtta attacattgt tgaatcaaat tactggtaaa tggttaggtg aataatttaa
     1501 ttttatgtat ttgatatcca acattgatcg acgtacgtac gtagtgaggt agaggcttag
     1561 gttaggaatc aatcgtgaat actatttcaa cgttgattcc cgcgagctga atattttttg
     1621 tgatgtatag taaaacgcat atgattatag gaatcatcat aaagattctt gtcatgaaga
     1681 acaatattaa accaaattat gcattacgag caaaataatt atacaaaaat actgataatt
     1741 ataacttggg tcattgtatt ttctgtgtaa tgtctactat cccttaatta gtcggtttaa
     1801 atcaggaaaa agtataatta atgacactca taattgtatc gttaagactt gaaagtgacg
     1861 gccaagaata caattaagag ccaattagtg atctttcata actttaaaaa tctcacaaaa
     1921 gtagaaaaaa aaaaattcca acttgatgac caagaataat actattaaag agctatttaa
     1981 gatgaaaccg cccgaaacca aaagcattcg atgggtacac cgattactgc ttttagcaac
     2041 accacggcgt gaccatcaag actaattaac taagaccaca ttttaaaaaa actattaata
     2101 attactacaa tttgtaatta aaaagatcaa cgagaaatgc cacgtggacg aatactagca
     2161 acgccaagtg gaaagagcgt tcgagagaac aaggcaaaac caaatacgcc cctagtattc
     2221 tacagatgtc gactggataa ttacaaaaga tttcaataaa cagtactaat taatttctag
     2281 tggtgagttt ttgtaaatat ctacttcttc caattaccag ctgctatata aatccccttc
     2341 tctgtttctc ttttcttaca tcacaatcac acaaaactaa caaaagatca aaagcaagtt
     2401 cttcactgtt gctcgagatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     2461 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     2521 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     2581 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     2641 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     2701 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     2761 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     2821 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc
     2881 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2941 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     3001 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     3061 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     3121 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     3181 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     3241 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     3301 gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt
     3361 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     3421 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     3481 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     3541 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     3601 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     3661 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     3721 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     3781 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     3841 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     3901 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     3961 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     4021 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     4081 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgccta ggtgagtcta
     4141 gagagttaat taagacccgg gactagtccc tagagtcctg ctttaatgag atatgcgaga
     4201 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     4261 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     4321 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     4381 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     4441 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     4501 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     4561 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     4621 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     4681 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     4741 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     4801 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     4861 gcgacaacat gtcgaggctc agcaggacct gcaggcatgc aagcttggca ctggccgtcg
     4921 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4981 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5041 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     5101 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     5161 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     5221 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     5281 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     5341 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     5401 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     5461 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     5521 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     5581 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     5641 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     5701 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     5761 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     5821 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     5881 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     5941 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     6001 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     6061 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     6121 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     6181 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     6241 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     6301 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     6361 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     6421 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     6481 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     6541 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     6601 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     6661 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     6721 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     6781 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     6841 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     6901 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     6961 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     7021 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     7081 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     7141 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     7201 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     7261 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     7321 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     7381 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     7441 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     7501 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     7561 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     7621 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     7681 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     7741 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     7801 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     7861 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     7921 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     7981 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     8041 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     8101 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     8161 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     8221 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     8281 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     8341 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     8401 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     8461 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     8521 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     8581 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     8641 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     8701 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     8761 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     8821 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     8881 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     8941 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     9001 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     9061 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9121 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9181 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     9241 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9301 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     9361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10141 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    10201 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    10261 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    10321 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    10381 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    10441 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    10501 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    10561 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    10621 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    10681 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    10741 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    10801 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    10861 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    10921 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    10981 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    11041 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    11101 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    11161 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    11221 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    11281 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    11341 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    11401 ctggctgg
//
