LOCUS       sequence-420666-       12411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by WOL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pWOL-GW
FEATURES             Location/Qualifiers
     source          1..12411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3424..3548
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3573..3578,3579..3596,3597..3603)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3657..4316
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3723..3742)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4658..4963
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4842..4861
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5004..5128)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5181..5888
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5913..5930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5913..5929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5922..5944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6132..6156
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7457..8086
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8515..9588
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9654..9848
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10084..10106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10192..10332
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10247..10266
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10347..10364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10518..11106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10598..10617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11193..11987)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc ctcacacacc acaccatcat tatctttgga aatttgtaac caactcaaga
     1381 ttttccaaac cgttttatct tcctctacaa aaatccaatt cacgttaaat ctatctcttg
     1441 ctcttgcttc ctccaaaaaa aaaaaaaaat cattcccaga tccatcgata tgaaattgta
     1501 tagaaaaaat ggtattcgat ccaagtttat tgtcttctat ttttcttagg ttaatttcac
     1561 tttattccag attcattgtt tgttttttct ttctcggaag agcacaatgt gagtttcact
     1621 ggcctctgtt ataaacatat atagaaatct gtaacaaaaa tcattactaa aattctgtga
     1681 catgtgcagc gatcaaagaa tcaatagcgg aaaaagaaac tacactgcat tcatctatga
     1741 ctgaaagctt ctgatcaagc catgaaatta aggtatccca aacacgtatc ttctctatgt
     1801 ttatcaatct tgctttaagt tctaattctg catatttcaa aggaaccata caagtgttcc
     1861 taaaatccat ttgaatattc aaaaacttct ctcaaatatc atgtagttat agaagctact
     1921 gtctctaagc gcacgagaga aagctacaca acccacgtca gtttccatct acacatataa
     1981 ggtaataata atattttcat gtatctttaa taatagctct atgttttttt ctgtattttt
     2041 cattataaaa ctcataacta tgttatcatt taatatggta ctaatttaat gggattgatt
     2101 tactattgcc tcaaacatgt aataatttaa tgattttttg tttttaacgt ttttagaaat
     2161 tcatgagcat tttaaatttg tggttaggtc ataacaattt gctattacaa aaaaaagaaa
     2221 cactctaaat aatataaaaa atagtttacc gtataatact agtagtaaat aaataatttg
     2281 attgttattc ataaattttg aattctaaaa tctcctgaat caactcatgc aattgtctta
     2341 agaattacac gtggataaat catgggctta tgagtcaggc ccatttaacc ggggtatttt
     2401 cgtagttaag agactagaat ggtgggtatt tcaggtaaaa ggtctatggg gccagatctg
     2461 cgctttgtcg cgatgtcatt atcgccaaag atatgcgata gcgactctcg tacaaagtct
     2521 ctcactcacc tatatttttt gttttcttat atttcaacaa aaaaacgttt tattttcctt
     2581 ttggtgtaag taaaaaaaca aaacaaaacg ttttatttct aaagttcaga aaacttattt
     2641 ataccaagga aaaaatagat aataaatttt gagaagttgg tgactatata ttacttcact
     2701 tattcaagaa atttaaacat ggtaaatgtt actttaaatg ttaaatgatg tataagaaat
     2761 gtaatgaaat tgaataaatg tagttttaaa gatgttttaa ttagtaagac aaacctagtt
     2821 agtgtcacaa taattatatt tttttttttg tcatccaaaa ttattaaagc tcaagtaaac
     2881 caatcctgag ggatattatt tacaaatgtg atatgatgcg gttcggtgcg gatcttccgc
     2941 gccaaattat acgcttttat attagcatta taaaaaatta tagataaaga gaagtttgtg
     3001 aattcttcat tgtcgctttg caatttctct aaatacacag taaataccga caattcggtt
     3061 agagaaaata tatctatttc gtataataat gttaactttg aggagatttt gggtaaaata
     3121 ataacttttg ttggatggat catatcatga gccattaaga aaaagtccaa aacttttctt
     3181 cttcaaagtt ggactcaagt tagaaaaaga aaaaagagct agagagatat aaaaatgaaa
     3241 agaaagttca tggcaaaaaa ctgatataga cagagacaca gagagagaga aacgtatctg
     3301 aagaaaatct aaaaaattcg attcaatttt tttcttactt ttaaaagcaa aaaatctcac
     3361 taaaacaaaa gaagaagaaa gaagaaagaa aatggaatac ctacatttga agtgctcgag
     3421 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     3481 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     3541 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     3601 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     3661 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     3721 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     3781 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     3841 ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc
     3901 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     3961 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     4021 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     4081 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     4141 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     4201 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     4261 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat
     4321 ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     4381 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     4441 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     4501 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     4561 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     4621 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     4681 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     4741 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     4801 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     4861 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     4921 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     4981 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     5041 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     5101 ctcgttcagc tttcttgtac aaagtggtgc ctaggtgagt ctagagagtt aattaagacc
     5161 cgggactagt ccctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga
     5221 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     5281 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     5341 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
     5401 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
     5461 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
     5521 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
     5581 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
     5641 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
     5701 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
     5761 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
     5821 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
     5881 ctcagcagga cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
     5941 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6001 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6061 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     6121 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     6181 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     6241 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     6301 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     6361 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     6421 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     6481 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     6541 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     6601 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     6661 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     6721 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     6781 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     6841 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     6901 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     6961 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     7021 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     7081 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     7141 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     7201 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     7261 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     7321 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     7381 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     7441 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     7501 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     7561 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     7621 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     7681 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     7741 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     7801 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     7861 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     7921 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     7981 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     8041 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     8101 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     8161 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     8221 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     8281 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     8341 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     8401 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     8461 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     8521 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     8581 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     8641 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     8701 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     8761 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     8821 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     8881 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     8941 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     9001 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     9061 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     9121 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     9181 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     9241 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     9301 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     9361 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     9421 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     9481 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     9541 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     9601 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     9661 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     9721 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     9781 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     9841 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     9901 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     9961 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    10021 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    10081 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10141 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10201 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10261 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10321 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10381 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10441 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10921 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11161 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
    11221 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11281 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11341 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11401 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11461 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    11521 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    11581 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    11641 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    11701 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    11761 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    11821 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    11881 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    11941 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12001 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12061 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12121 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    12181 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    12241 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    12301 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    12361 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
