LOCUS       sequence-420667-       12481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by SCR promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pSCR-GW
FEATURES             Location/Qualifiers
     source          1..12481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    3494..3618
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3643..3648,3649..3666,3667..3673)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3727..4386
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3793..3812)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4728..5033
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4912..4931
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5074..5198)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5251..5958
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5983..6000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5983..5999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5992..6014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6202..6226
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7527..8156
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8585..9658
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9724..9918
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10154..10176)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10262..10402
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10317..10336
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10417..10434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10588..11176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10668..10687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11263..12057)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgattgtga tcctctgcaa caaagcggat tttgctggtg ttgaatggat aagggataga
     1381 ggaagaggac tttgtttatc agaaaccttt tgatgggcct taatgggcct ataaactgta
     1441 actctgtagc gctttgccaa caagagactt tttaaggttt ttgttgccaa acagatattt
     1501 gcatttgggc tatgtaatgt tagaattatt ttataatgta tgctattgct agatattgtt
     1561 taagtgcatt tgtgatttac aaacatttca tttttatttt ggttttaatg agcatttcta
     1621 ttatagagac tttgatgtta ataaatggtg ttctaagata tattaaaata ttttatatac
     1681 tttcttaaaa ttggataaat tttgggaaaa tccttaatat cagttaaatt gaagataaag
     1741 agtattaaaa aaaactatgt agtaaaatac atttcacatt ttttgtgtat aatagtacat
     1801 ggtattcgtt aagatcactc aaaaattaac aaattaagtc taaaagggca gaaaagacta
     1861 ttcaaatatg gacttggaga aagacattca gctttttacg ctgagaaact ttcatattga
     1921 gccgtgtgtt tgtgttgtga agagaagtaa taaaaaataa tttgaagtga aaaaggagaa
     1981 gaaaaaataa gatcgtagaa agcgtggatg gtttcttctt gggttcactg ccatgcgatt
     2041 attaaattgg ccatggggct agtgtttgac gtacaaaagt ctaaaaattg tcagtcaaac
     2101 aggtccaaaa ctttgtaaga aaaataatat aataatagca aattttctaa aaattgttaa
     2161 aaaaagaaca aaagggaaaa gatgaggatg cagatgaaag caaaatgtca aacactagtt
     2221 tcagatttta tcgggaactg gggtttgaca gttggtgtat gtatgtaatg gcctctcatc
     2281 aaaacatgtg catctttttc cttttttgtt atttactgtt ttagctctac gtcttgtcca
     2341 attcctctca agtaaaatgc ctttaatatg atactaatat acaaggggac taatgctttt
     2401 tcccttttct tatccttgtt ttgtctaaat ctttacttgg attcctttat ttttctcctc
     2461 tctttagatt agtacggttt aaggaatacc atctttctaa ttttagcaca aaattgcaag
     2521 ttggtgcccc atcttagtaa gcacatcgta ccacactttg attgtgtgag agacttcttc
     2581 atcccatctc tcataccaaa cctaaatcaa atgactagtg gtgcaacctg ctgactccat
     2641 atgaccataa ctaataaatc ggtttatgaa tccaactcat gtagctctat agaatagaaa
     2701 cccattcatt tcacataatg aactgaatct gacattttat ttacatcatt tactactcaa
     2761 ttttgtaatt agcaagatca tctttttcat tattcaacaa ttttgatatt ccataattta
     2821 ttaactttgt catacatcat aatattctga aattttgtta tatattgtac cggttccacg
     2881 aaatagagct ctattattat agaccaaaca aacaaaatat tatcttcttg tggttagttc
     2941 gagagagagg tcaagaagaa acgaaatgga tcggcaaacg gaagacgtca aacacacaac
     3001 gacgaacatt ttccgatcac ccacctaatc tcttcccatt tttattattt ttcaaaactc
     3061 aaattaatta agaagaaaaa aacagaaaca gagagagaaa gagttaagat gaatagagat
     3121 agaaagagtc attaaatgta cgaagcgaca ttcacaataa ttcgaaaggt ggaagacgac
     3181 ttagatacgg ccaggcttca ctgtcctcct cgtcctcctc aattacccct aacccctttt
     3241 tccgggattc atctccaacc cacatccttc caaattctca ccccctcact gagtttttgc
     3301 tttttctcct catcggagat cgtgaagacg atcaagtaat ttaagaatcc caccattgat
     3361 aaaagagtct agcttttcta ctaccaaacc tttttctgtt tggaaatttt cgattttgga
     3421 tttaaccctt ttcttacctt atttataacc atgcaatctc acgaccaaca acccttcaat
     3481 ctccctcgag atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     3541 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     3601 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     3661 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     3721 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     3781 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     3841 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     3901 tttattcaca ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa
     3961 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     4021 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     4081 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     4141 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     4201 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     4261 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     4321 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     4381 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     4441 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     4501 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     4561 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     4621 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     4681 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     4741 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     4801 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     4861 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     4921 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     4981 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     5041 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     5101 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     5161 ttttacgttt ctcgttcagc tttcttgtac aaagtggtgc ctaggtgagt ctagagagtt
     5221 aattaagacc cgggactagt ccctagagtc ctgctttaat gagatatgcg agacgcctat
     5281 gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt
     5341 agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc
     5401 gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt
     5461 acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata
     5521 gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg
     5581 gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc
     5641 cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa
     5701 ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg
     5761 ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac
     5821 ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca
     5881 ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa
     5941 catgtcgagg ctcagcagga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
     6001 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6061 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6121 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct
     6181 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     6241 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     6301 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     6361 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     6421 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     6481 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     6541 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     6601 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     6661 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     6721 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     6781 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     6841 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     6901 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     6961 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     7021 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     7081 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     7141 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     7201 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     7261 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     7321 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     7381 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     7441 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     7501 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     7561 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     7621 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     7681 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     7741 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     7801 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     7861 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     7921 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     7981 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     8041 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     8101 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     8161 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     8221 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     8281 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     8341 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     8401 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     8461 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     8521 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     8581 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     8641 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     8701 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     8761 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     8821 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     8881 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     8941 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     9001 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     9061 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     9121 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     9181 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     9241 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     9301 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     9361 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     9421 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     9481 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     9541 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     9601 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     9661 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     9721 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     9781 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     9841 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     9901 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     9961 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
    10021 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
    10081 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
    10141 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10201 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    10261 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    10321 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10381 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    11041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11221 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
    11281 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    11341 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    11401 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    11461 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    11521 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    11581 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    11641 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    11701 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    11761 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    11821 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    11881 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    11941 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    12001 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    12061 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    12121 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    12181 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    12241 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    12301 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    12361 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    12421 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    12481 g
//
