LOCUS       sequence-42067-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CXXC1
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             309..326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(379..397)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      393..440
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(564..583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttaac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggccagatca aacggtcagc ccgcatgtgt
      121 ggtgagtgtg aggcatgtcg gcgcactgag gactgtggtc actgtgattt ctgtcgggac
      181 atgaagaagt tcgggggccc caacaagatc cggcagaagt gccggctgcg ccagtgccag
      241 ctgcgggccc gggaatcgta caagtacttc ccttcctcgt gatgacgaag cttgcggccg
      301 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      361 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      421 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      721 gccctgatag acggtttttc gccctttgac
//
