LOCUS       sequence-420670-       11628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by LBD16 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pLBD16-GW
FEATURES             Location/Qualifiers
     source          1..11628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    2641..2765
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2790..2795,2796..2813,2814..2820)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2874..3533
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2940..2959)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3875..4180
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4059..4078
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4221..4345)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4398..5105
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5130..5147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5130..5146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5139..5161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5349..5373
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6674..7303
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7732..8805
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8871..9065
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9301..9323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9409..9549
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9464..9483
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9564..9581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9735..10323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9815..9834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10410..11204)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcggaaga acttataaaa taacttttct aaaattaaaa taaaaataaa gaatgaatga
     1381 aagaagatag acaaaattga ttattaattg agtttgcttt gaagctccaa gaaccaaacg
     1441 tatccgggta taaaataata cgagataaag tgatagaaga cctaagacta cctcattact
     1501 aaaaatatgg atatttttct atacaaatcc acgaatgaaa agaaaaagaa gttggactaa
     1561 ttaaattgaa atccaataat tgtaaatata atattaaaac ccaataaatt agaagtctca
     1621 tgttgcagtc tccattattg attgtctcct tagaatccaa catgtgaccc tccattgtct
     1681 accattcaca ccctttggct cactcgataa atttggacat taattgcatt cattttattt
     1741 gtaagaacca gaatctcagt agatgttatt tgtgttgagt aaactgttgt tatttataga
     1801 ctaggctatt cttcgtatac actttgatac tgatttttag ttcatcatct atctaccgac
     1861 taaatatagt gtttctatgt ggcttctcct caaatgtcaa atttatttct ttaatgttgt
     1921 ttgagtttca aacttgcaaa gtgcaaacca attttggttg ttatactaaa accacacata
     1981 taattaaaga cacccactat agcataaaat tcgtcgtgta agcaatttta ctatagtcta
     2041 aactccataa aaggacgtcg aaaaatattg tgatattgga attatagtgt cagtttgttt
     2101 ttctttgggc aaatggattg tagtttcatt atatattaca atttataaac gaaacgataa
     2161 gtatggctca tactttagtg atggagaatt aaagtatttt atattcagag atgacacaat
     2221 atggtagacc aaaaataact aattaaaata gtatcatatt agatatttat atgtgattgt
     2281 aaatttgatc attgagtgaa cattttaaat tagtatgccc aagaaagaaa agaaaaaaaa
     2341 aaaactcttt ctctctcatg ataatgttcc ttttataaaa tccacccaaa gacaccaaac
     2401 acttctccat catcatttca tttcttgttc tttttccacc atcaccattt cctagttcgc
     2461 tcgtgcacat ctccgacgat cccaattcta aaatcatcac ttcttccaat cttttcaaca
     2521 tctcttcaaa atatccagtc aacaaaaaaa aaagtactta aaacaaggaa accccttctt
     2581 tctttttgta catataacat aaaaatcatc acttttttgt tcgtttcgcc gctcgagatc
     2641 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     2701 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     2761 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2821 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     2881 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2941 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3001 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3061 ttgcccgcct gatgaatgct catccggagt tccgtatggc aatgaaagac ggtgagctgg
     3121 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3181 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3241 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3301 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3361 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3421 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     3481 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     3541 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3601 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     3661 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3721 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3781 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3841 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     3901 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     3961 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     4021 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4081 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4141 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4201 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4261 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4321 gttcagcttt cttgtacaaa gtggtgccta ggtgagtcta gagagttaat taagacccgg
     4381 gactagtccc tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     4441 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     4501 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     4561 taatattctc cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat
     4621 gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata
     4681 acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca
     4741 aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt
     4801 atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg
     4861 ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt
     4921 tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag
     4981 aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga
     5041 cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc
     5101 agcaggacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     5161 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5221 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5281 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     5341 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5401 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     5461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     5521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     5581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     5641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     5701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     5761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     5821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     5881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     5941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     6001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     6061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     6121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     6181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     6241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     6301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     6361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     6421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     6481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     6541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     6601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     6661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     6721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     6781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     6841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     6901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     6961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     7021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     7081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     7141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     7201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     7261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     7321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     7381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     7441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     7501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     7561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     7621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     7681 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     7741 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     7801 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     7861 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     7921 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     7981 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     8041 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     8101 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     8161 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     8221 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     8281 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     8341 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     8401 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     8461 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     8521 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     8581 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     8641 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     8701 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     8761 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     8821 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     8881 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     8941 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     9001 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     9061 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     9121 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     9181 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     9241 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     9301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     9421 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     9481 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     9541 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10381 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    10441 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    10501 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    10561 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    10621 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    10681 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    10741 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    10801 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    10861 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    10921 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    10981 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    11041 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    11101 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    11161 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    11221 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    11281 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    11341 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    11401 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    11461 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    11521 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    11581 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctgg
//
