LOCUS       sequence-420671-       23876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing a 13,748 bp fragment of the P. tricornutum 
            chloroplast genome. The fragment is flanked by SapI recognition 
            sites, allowing it to be released from the cloning vector pSAP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23876)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 23876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP7
FEATURES             Location/Qualifiers
     source          1..23876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10925..11027
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11028..11687
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11094..11113)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    11907..11928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11943..11948,11949..11966,11967..11973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11981..11997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11986..12008
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12005..12021
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12005..12021
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             12017..12292
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     misc_feature    12064..12120
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(12122..12141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12123..12141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12148..12165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12148..12164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12157..12179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12372..12391)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12459..12492
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    12510..12908
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     CDS             complement(13972..14943)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKDGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(14943..16118)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFTPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(16514..17188)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(17189..17336)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    17390..17893
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    18055..18305
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(18308..19063)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      19154..19373
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      19441..19972
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      19449..20063
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 gggcaaccaa gtgttatcat aaatgcagaa ggtttacgaa caacaccatc tattgttgct
       61 tatacaaaaa aagaagaatt attagtgggt caaattgcaa aacgtcaagc agttattaat
      121 ccagagaaca ctttcttttc agttaaaaga tttattggtt caaaagaagg agaaatttca
      181 aaagaagcaa aaaaattacc ttataaggta agtaatgatc aaaataataa tattaaaata
      241 aagtgccccg cattaaatag ggatttcagt ccagaagaaa tttcagctca agttttaaga
      301 aaattaatta atgatgcaac agattatcta ggacaagagg ttactcaagc tgttattacg
      361 gttccagctt atttcaacga ttctcaacga caagcaacta tggatgctgg aaaaattgct
      421 ggcgttgaag ttttacgaat cattaatgaa ccaactgctg catcattagc atacggttta
      481 gataaaaaac aaaatgaaac cattttagtt tttgatttag gtggcggtac ctttgatgtt
      541 tcaattttag aagtaggaga cggaattttt gaagttctat cgacagcagg tgatacaaat
      601 ttaggtggtg acgattttga taatgttttg gttaattggt taattaatga ttttaaagaa
      661 aaaaataata tagacttagg gaatgatgtt caagctttac aaagattaac agaagcagca
      721 gaaaaagcaa aagttgagtt atcaacagtc gaaaaaacaa caattcattt accatttatt
      781 actgctgaca aaactggccc aaaacatatt gaacaagaat taacaagaga tgtgtttgaa
      841 aatttatgtc aagctcttat taatcgttgc cgaatcccag ttgaaaaatc actaaatgat
      901 gcacgccttg ataaagccga tattaatgaa attgttttag ttggaggttc aactcgaata
      961 ccagccgttc aaaatttggt tgaatcatta acaggtaaaa aacctaatca atcagtcaat
     1021 ccagatgagg tagttgctat cggtgcagca attcaagctg gcattttagc tggggaaatt
     1081 aaagatattc tattattaga tgttacacca ttatcattgg gagtagaaac gcttggtgga
     1141 gtaatgacaa aaattattgc tcgaaatacg acaattcctg taaaaaaatc ggaaatgttc
     1201 tcaacagcag tggataatca aacaaatgtc gaaattcata tcttacaagg tgaacgtgaa
     1261 cttgtcgcag ggaataagag tttaggtaat tttagattag atggtattcc agctgccgat
     1321 cgcggtgctc ctcaaattga agtaacattc gatatagatg ttgatgggat attatccgta
     1381 aaggcacgtg aaaaagaaac aggtgtagaa caagctgtta caattcaagg cgcgtcaaat
     1441 ttaaatgaaa aggaagtgga agaaatgcta gttgaagcag aaaaatttgc ctcagcggat
     1501 aaggtaaaac gtgaaaatat tgatgtaaaa aatcaggcag aagcattatg ttttgaagct
     1561 gaaaaagaag tttcgtcatt taacgacact gttcctgaaa ataaaaagca aaatattaag
     1621 acgttaattg aaaatattcg tcaagcagtc cagactgaca attttgaaac tttaaaagaa
     1681 caaatggagg aattgaaaac agctatgaaa gacatagttg cactgaaatc aactaatgat
     1741 acagctacag atccaatgtc gaatttaaat gatttataat ttatattgtt caaacgaatc
     1801 ctaagattcg tttgaacttt taacatcatc aacgataata cactctgtag ttaaaatcat
     1861 acttgcaatt gaagtagcat tttgtaatcc tgatcttgta actttagctg gatcaactat
     1921 tccctctgta tacatattac caaatgtatt tttagcagca ttataaccaa tttcaaattc
     1981 ttgttgctgt actttttcaa taataacagg accattaata ccagcatttt ccgcaatccg
     2041 ccgcaaaggc gctaagatcg cccgcgatat aatcattgca ccgattaatt catcttcttt
     2101 tagattattt tttgcccacg taaccaaatt ttccgacaaa tgtgttaagg ttgcaccacc
     2161 acctggcacg ataccttctt ctacggctgc tcgagtagca ttaattgcat cctctaaacg
     2221 taatttttta tccttcattt cagtttcagt aacagccccc acacgaatta cagcaattcc
     2281 accagataat ttggcaattc gatcctgtaa tttttctttt tcataacccg tatcagcaat
     2341 attaacctgt ttacgcaatt gttcgcatct aatattaatt tcatttaatg tttgtccatc
     2401 cgaaacaatt gttgttgaat ctttattgac aataattcgt cgagcttggc ctaataaatt
     2461 taattgaata ttatctaaac ttaagccagc atcctgtgta ataactgtcc caccagttaa
     2521 aatagctatg tcttccaaca tttgctttct tagttcacca aaccccggtg cacgaactgc
     2581 aacaacatta ataattcctc ttaatttatt taaaattaat gttgctaatg cctctttttc
     2641 aacatcttca gcaatgatta agagagggcg ttttgtttta gttatttgct ccaaaattgg
     2701 tagtaaatct tgttgaacta gtgtaatccg cttatctgtt agtagaatat aaggattatc
     2761 ataggaaact tccatctttt cagtatttgt aataaaatat ggtgaaataa aacctttttc
     2821 tagcttcata ccttcagtaa tttctaattc tgtaacaata ccttttcctt cttctaacga
     2881 aattacacct tcttttccaa cttttgataa tgcatcggca attaaagatc caataatttc
     2941 atcatttcct gctgaaattg aagcaacttg ttgaatcgat tgaatatcct ctactggttg
     3001 agcgaattca ttaatttgtg ttaccaaata ttgagatgct ttttccattc ctaatttaat
     3061 tgaaattgga tttgcgcctg ctgcaacatt tttcaatcct tcttttacca tcgcatacgc
     3121 taaaactgtt gcagttgttg ttccgtcacc tgcgacatca ttagtctttg aagccgcttg
     3181 gcggattaat gaaacgccgg tattttctat atgatctaat aatttgattt ctttcgcaat
     3241 tgttacacca tcattaacaa tttgaggcga accataagat ttttctaata caacatttcg
     3301 accttttggt cctaatgtaa ctgaaacagc ttcaaccatt atttccattc cacgttctaa
     3361 ggctcgtcga gcattgtctt gatataaaat tttctttgcc atattttaaa aataaatttt
     3421 gttaattttg aatgcgaact aagtgaagta taaatttaat tgaagagtta gtttttttgc
     3481 aagttgaaaa ccaagtttcg ttaatttttt ttcaacttta cgaaagtata attttttagt
     3541 actatatatt taagtaaata tattttgggc ttgtagctca gtggactaga gcacatggct
     3601 acgaaccatg gtgtcagggg ttcgaatccc ttcttgccca atactaaaat ttaaaattta
     3661 caaacagatt tggggtgtcg ccaagtggta aggctgtgga ctttgactcc gccattcgta
     3721 ggttcgaatc ctgccacccc agttaggaat atttatactt aaagctttag tagtaaaaac
     3781 tcgtttttaa tttacagaat tcacatagtt gaagtaagat acagttatta caatttttgg
     3841 tcttgaataa ggataaaatt atggaagcta aaattcaatt tataaaaggt cttgatgaaa
     3901 aagttttacc ggatgttcgt ttaactcgat caaaagatgg cagtacgggt acagcaacat
     3961 ttcgctttaa aaatccaaat atattagata aaactactgc aaaagagggt gaaattaccg
     4021 gtatgtatct aattgacgaa gaagggattt tagaaactcg cgatgtcaat gcaagatttg
     4081 ttaatggaaa accagaggcg attgaatcga tttatattat gaaaagtcct gaagcctggg
     4141 atagatttat gcgatttatg gaaagatatg gggaaacaaa tggtttagta tttagtaaag
     4201 caagttaatt agaaaattga taataactca aaaatgcaaa tttcactaaa atggattaaa
     4261 gaattaattg atattgaaaa tgttgattta gatgatttaa ttgaaaaatt aacacttggt
     4321 ggatttgaag ttgaagaaat cttggaattg gaaattaata atgaaaaaca aattgcgtta
     4381 gaaatttctt caacagcaaa ccgttcggat agtttatcga ttcaaggtat ttcagttgaa
     4441 attgcgtcat tatttaataa acagcctaaa gtttgtaaat atttcaattc atctttaaat
     4501 tggcaacaaa aaattaaaaa tttgacgact atcagaacaa gtaaaacaga gtgtctaatg
     4561 tttactgcag tgatattaga aggtttagaa gatcttactg ttccaaaatg gattcaaaat
     4621 aaattagtaa gttcaggaat tatgccttta aataatttgg tagattttca aaattatatc
     4681 ttattggaga ctggctatcc ttttgcattt tatgatttag ataaagttta ttcaaaggtc
     4741 cgaactccaa cattctcttt atcaatagaa aaggcagaaa acggtagtga attttttgcc
     4801 agtaatcaga taaattataa attagacaat tccatttttt tagtccaagc aaataatatt
     4861 ccaataagca tagctggaat tattgaaaat gatgaaatca tatgtcattc taaaactagt
     4921 tcgttattaa tagaaggcag tatttttagt gcatctaaaa ttcgtcagca gtcgagaaaa
     4981 ttggcaattc gaacagatcg ttcagcccgt tatgaaaaat ctctaaagag tacatattta
     5041 attgaagctt tgtatagatt aatctcttta ttacgaattt ccaatccaag tttagtctat
     5101 aaatttcatt catcaaataa ggttcttgaa aaagttttaa aaccgatcgt gttgaactat
     5161 aaaacaatag ttgaaatttt aggacctata aaaaaacgta caaacgaaca tttaatttat
     5221 atttcacctg aaaccgtaac tgactattta aagcgcttaa attttaaatt tctattcgac
     5281 tcttcgacat taaattggga agtgactatt ccatctatac gcagtgatga tattactcgt
     5341 gaaatcgatt taattgaaga agttggtcga ttgcatggat ttaataattt tttaacaatg
     5401 ttaccacaaa ttaaatccgt tggtagagca gattttagtt atcaaacacg taaaaaaatt
     5461 acctcatatt tattaaatat gggattaact gaattaattc actattcttt agtaagcaat
     5521 gaaacatttt taaaaaatga aattaaatta gttaacccac ttttatctga ctgttcgact
     5581 ttaagagtca gtttacttcc aagtttatta atgacaattc aggaaaatct taaacaagga
     5641 aattcgattt tagaaggatt cgagtatggt catgtatttt ctggaaatat cgaaacaaca
     5701 ttaacagaaa tagaatatgt aggagggata tttggtggta ctaaaataaa atcctcatgg
     5761 tttgaaaaag gccagtcttt aaaatggttt gaagcaaaag gaaaaattga aaaattattt
     5821 caacagttaa acttaggaat tcattggcga attaattcac aaacttatac aaaaaaattc
     5881 ttgcatccat atcgtagtgc tgaaattttt ctttcaagtg gtaaaaatat aggtgtattt
     5941 gggcaacttc acccactact tgccaataaa ctaggacttt cgagtgaaat ttatttattc
     6001 gaactaaatt tagaattgat tcaacagagt cttcaacaaa ataaattaac tatttatagt
     6061 caatattcag tttacccaaa aatagttaaa gatttatcat ttattattaa aaaaaatatt
     6121 aagtttgatg aattagaaaa aattatttat gcaaatggaa cggaattttt atcgcaaata
     6181 aatttattgg atgattataa aggtgaattt attccagaaa agcatactag tctctgtttg
     6241 caattaactt ttcaatcgaa taaaaagaca ttggagaata aagaaattga cagaattgtt
     6301 aaaaatctta aacgtgttct ggaacttaaa gtccaagcta tacttcgtga gtgattctat
     6361 aattttttat agaattaccc acctccaaca atcgtcacaa tttcaatttt atcattattt
     6421 tgaataaaaa tagtattcca attttttttc aaacagatta aattattgta ttctaggact
     6481 aatagtgctg tatcgtagtc aaaataatta attagctcaa agagcgttaa atctccgtca
     6541 gttgaatatt cctctccatt taaaacaaag tgtttgctat ttggcatatt ttcttagata
     6601 aaatttattt gaaatatagt agacgtaaca gaatagaaac tggtattatc ttgtttacaa
     6661 cagttaggcg ggtgtggcca agtggttaag gcagcgggtt gtgattccgc cattcgccgg
     6721 ttcaagtccg gtcactcgcc cttgttaaag tttatcgatt tcagtacttg taaaaaaatt
     6781 atttataatt ttaatcaatc aatttttatg tcacgttatc gaggacctaa attaaaaatt
     6841 agtcgacgat taggaacatt gcctggttta acaacaaaaa aatctaataa gttgaaccgc
     6901 ccgggcaagg acggaaatac cgatattgga aaaaaattaa cggagtacgg tgtacgtttg
     6961 gaagagaagc aaaaattaaa atttaattac ggcttaactg aaaaacaact ttttagatat
     7021 gtaaaagaag cacgccgccg ccaaggtgtt acaggtttaa ttctattaca gttattagag
     7081 atgcgtttag atactctttg ttttacttta ggttttgcta agagtctagc acaagctcgt
     7141 cagttagtta atcatggtca tatcactatc aacaaaaagg tcgttagcat tcccagcttt
     7201 caatgccgtt taaacgatat tattagtata aaagaaaaaa gtagttcaaa aacattagtt
     7261 gaaactaata ttaaaaataa tcaagttcgt gaaattccaa cacatttaag atttgatacg
     7321 gtaaaattag aggcaatagt tttagattat tgcgatcgga atgatgtatc cttacaactt
     7381 gatgagttac ttgttattga gcactattct cgccgttaat taaatttttg tcggatccat
     7441 ttttacttca aattatacta attatccttt gattttcacg caaacaatta atttatatat
     7501 tgaatttaag catatgttaa aattacgatt aaaacgaaca ggaagaaaac gatctccatc
     7561 atatcggtta gttgttatgg aaaatacagc acgacgagat ggacgtccaa tcgaagaact
     7621 tggctattat agcccaatca caaaacaata caagtttgat gtagaaaaaa ttaaaaagtg
     7681 gttagatttt ggtgtaaaac caactgaaac agtttcatcg ttattgagaa aagcggagat
     7741 tattagctag ttcttggaaa catatatgtt tccaagaact agctaataat ctttttacac
     7801 atagacgact taattaagtc aaaataagtt ataattttat atgaatttct ataaataata
     7861 ctaattaaaa attatgtcac attttacgca catgaaaacg cgatttcaaa acttattcta
     7921 tctcgaaaaa gcattaaatc gactaaatat tgctcataaa aaggaagaaa atgctgattt
     7981 aaactcgtac aatataaatt tagttattcc gcaatcgaat ggttacaaca ttgaatttgt
     8041 ttggcatgag gacgagtacg aactagttgt agatatgagt ttttgggaac aaccttatcc
     8101 agttgaaagt tttattgata aaatttcaca acaatatgct ggcgaagtga ttattggtga
     8161 aagtcaaaaa attggtttcc aaccaattaa atatcaacaa aatgttgatg gttcgaatac
     8221 attaatatta gaacgctgga acaacgaaaa aattgttgaa actgtataat atttcaatat
     8281 ccaatgattc attggatact ggaaacaaga ttgtttttta taagttttag ttagtcttat
     8341 aaaaacttta aaaataacga agaaaaattg agagggcttt ataggtttct aaacctttaa
     8401 tagaattaaa ggtttagaag aactagaaat taaccattaa cagctggagc tgttaaagca
     8461 actggtaata cttcacctga agctaaatct aatgggaagt tgtgcgcgtt acgttcgtgc
     8521 attacttcca tacctaaatc agcacggttg atgatgtcag cccatgtgtt gataacacga
     8581 ccttgagaat ctacaactga ttggttgaag ttgaaaccat ttaagttgaa cgccatagta
     8641 ctaacaccca tagcagttaa ccagatacct acaactggcc ataaagctaa gaagaagtgt
     8701 aaagcacgag agttgttaaa tgaagcgtat tggaagatta aacgaccaaa gtaaccgtgc
     8761 gctgcaacga tgttataagt ttcttcttct tgaccgaatt tgtaaccgta gttagttgat
     8821 tcgttttcag ttgtttcacg aattaaagaa gaagttacta atgaaccgtg catagcagag
     8881 aataaagaac caccgaatac acctgcaaca ccagccatgt ggaatgggtg cattaagatg
     8941 ttgtgctcag cttggaatac aagcatgaag ttaaaagtac cagaaatacc taaaggcata
     9001 ccatcagaga atgaaccttg accgattggg tatactaaga atacagcaga agctgctgct
     9061 actggcgctg aaaacgctac gaaaatccaa ggacgcatac ctaaacggta gcttaattcc
     9121 cattcacgac ccatgtaaga cgcaacgcct aataagaagt gtaatacgat taattggtaa
     9181 ggaccaccat tgtataacca ctcatcaact gaagctgctt cccaaattgg gtagaagtgg
     9241 ataccgatag cattagaact agggataact gcaccagtga tgatgttgtt accatataat
     9301 aaagaacctg caacaggctc acgaatacca tcgatatcaa ctggaggagc tgcgatgaat
     9361 gcaatgatgt agcaagaagt agctgttaat aatgtaggga acattaaaca accaaaccaa
     9421 ccgatgtata aacggttttc tgtactagta atccaaccac aaaaacgttc ccataaagaa
     9481 acgctttgac gtctttctaa agttgctgtc ataaaaaatt tgtataatta taattcttcc
     9541 caagtgttta acttgttaaa tataattata aaaccaataa gatgttacca tcactaattt
     9601 attttataat tttaaaaaat tccaattcaa aatcaaaaac tgccctattc gtagtaccac
     9661 caataaaaag ttttttctct ttttgaataa tccagatctg agaatacgtt atataactta
     9721 tcaacgtact caacatcaag aagaaaaatc cggagtaaat aatttgaatt cctggatctg
     9781 ttttaatctg taaacccgtt gaactaataa tttcaattaa ggttaatggt tgttttaaat
     9841 taattatttc gtttaattcg atattaccta aaaactggcc ggtttcattg tagattgaac
     9901 aatatccttc aagattatca atgatagtaa ctactccttc cgtcaaggat ttgttagttg
     9961 aaatccacgt tagccatatt ttttgattct ctgaaaaatt tattaatgga tactcaataa
    10021 tttcgttggc cattgtttta aagcgtaacc caactaaatt ccaagaagag cgggcttcgc
    10081 cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccac atcatggttc
    10141 tgtgttcatt aggttgttct gtccattgct gacataatcc gctccacttc aacgtaacac
    10201 cgcacgaaga tttctattgt tcctgaaggc atattcaaat cgttttcgtt accgcttgca
    10261 ggcatcatga cagaacacta cttcctataa acgctacaca ggctcctgag attaataatg
    10321 cggatctcta cgataattgg agattttccc gactgtttcg ttcgcttctc agtggataac
    10381 agccagcttc tctgtttaac agacaaaaac agcatatcca ctcagttcca catttccata
    10441 taaaggccaa ggcatttatt ctcaggataa ttgtttcagc atcgcaaccg catcagactc
    10501 cggcatcgca aactgcaccc ggtgccgggc agccacatcc agcgcaaaaa ccttcgtgta
    10561 gacttccgtt gaactgatgg acttatgtcc catcaggctt tgcagaactt tcagcggtat
    10621 accggcatac agcatgtgca tcgcatagga atggcggaac gtatgtggtg tgacctgaac
    10681 agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc tccccaatcc aggtcctgac
    10741 cgttctgtcc gtcacttccc agatccgcgc tttctctgtc cttcctgtgc gacggttacg
    10801 ccgctccatg agcttatcgc gaataaatac ctgtgacgga agatcacttc gcagaataaa
    10861 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
    10921 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
    10981 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
    11041 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
    11101 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
    11161 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
    11221 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
    11281 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
    11341 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
    11401 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
    11461 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
    11521 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
    11581 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
    11641 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
    11701 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
    11761 atggcagaaa ttcgatgata agctgtcaaa catgagaatt ggtcgacggc ccgggcggcc
    11821 gcaaggggtt cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactag
    11881 aaagcgggca gtgagctcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    11941 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    12001 cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaga ctatagaata
    12061 ctcaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
    12121 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
    12181 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    12241 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagct gaatggcgaa
    12301 tggcgcctga tgcggtattt tctccttacg catctgtgtg gtatttcaca ccgcatatgg
    12361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
    12421 acacccgctg acgcgaaccc cttgcggccg catcgaatat aacttcgtat aatgtatgct
    12481 atacgaagtt attagcgatg agctcggact tccattgttc attccacgga caaaaacaga
    12541 gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgtttc ctttcttttc
    12601 agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac
    12661 cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc
    12721 accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac gtgcgtggag
    12781 gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat tgatctgcat
    12841 caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat acggggcaac
    12901 ctcatgtccg agctcgcgag ctcgtcgaca gcgacacact tgcatcggat gcagcccggt
    12961 taacgtgccg gcacggcctg ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg
    13021 ttgtggataa gcaggacaca gcagcaatcc acagcaggca tacaaccgca caccgaggtt
    13081 actccgttct acaggttacg acgacatgtc aatacttgcc cttgacaggc attgatggaa
    13141 tcgtagtctc acgctgatag tctgatcgac aatacaagtg ggaccgtggt cccagaccga
    13201 taatcagacc gacaacacga gtgggatcgt ggtcccagac taataatcag accgacgata
    13261 cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtt
    13321 ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga
    13381 ccgacgatac gagtgggacc atggtcccag actaataatc agaccgacga tacgagtggg
    13441 accgtggtcc cagtctgatt atcagaccga cgatacgagt gggaccgtgg tcccagacta
    13501 ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat
    13561 acgagtggga ccgtggttcc agactaataa tcagaccgac gatacgagtg ggaccgtggt
    13621 cccagactaa taatcagacc gacgatacga gtgggaccat ggtcccagac taataatcag
    13681 accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg atacgagtgg
    13741 gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
    13801 aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga
    13861 tacaagtgga acagtgggcc cagagagaat attcaggcca gttatgcttt ctggcctgta
    13921 acaaaggaca ttaagtaaag acagataaac gtagactaaa acgaggtcgc atcagggtgc
    13981 tggcttttca agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg
    14041 agacctgtcc aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc
    14101 aaactgatgt cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt
    14161 taaaagagtg ataacttctt cagcttcaaa tatcacccca tcttttttct gctcatgaag
    14221 gttagatgcc tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc
    14281 acctgaccgg gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa
    14341 tttggcggtg ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag
    14401 cgcagaaata tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc
    14461 ataagcactt gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc
    14521 atccagctcg ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg
    14581 gcgactccca tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg
    14641 ttgaccagtc agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc
    14701 ctggtcacgt tcattacctg accatacccg agaggtcttc tcaacactat caccccggag
    14761 cacttcaaga gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg
    14821 agccattact cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa
    14881 cgaagtatct tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt
    14941 cattatctaa tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt
    15001 tcccaaatag aaagagcatt tctccaggca ccagttgatg agcgttgatc aatggcctgt
    15061 tcaaaaacag ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca
    15121 ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga
    15181 gagccattgc tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta
    15241 agatcaacgt tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag
    15301 tcaaacaact cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc
    15361 gtgccgatac ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca
    15421 gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat
    15481 ttgcccatta actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc
    15541 cccggccagc aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa
    15601 ggcaggagag tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag
    15661 gctgttccct gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga
    15721 tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc
    15781 ccgatcaccg gtgtaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc
    15841 atatgattaa tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc
    15901 atatccgggt gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc
    15961 ccaactaaat ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg
    16021 ctgtcatcat taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg
    16081 cactggttaa gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt
    16141 tttttattaa atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa
    16201 accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg agataagaag cgcacatacc
    16261 tcagtcactt attatcacta gcgtcaagtc cagactcctg tgtaaaatga tttataaggg
    16321 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    16381 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
    16441 tgtgcggtat ttcacaccgc atagatcggc aagtgcacaa acaatactta aataaatact
    16501 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
    16561 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
    16621 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg
    16681 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
    16741 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
    16801 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
    16861 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
    16921 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
    16981 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
    17041 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
    17101 ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
    17161 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
    17221 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
    17281 cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa
    17341 ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg atcgcttgcc
    17401 tgtaacttac acgcgcctcg tatcttttaa tgatggaata atttgggaat ttactctgtg
    17461 tttatttatt tttatgtttt gtatttggat tttagaaagt aaataaagaa ggtagaagag
    17521 ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact
    17581 ttacatatat atttattaga caagaaaagc agattaaata gatatacatt cgattaacga
    17641 taagtaaaat gtaaaatcac aggattttcg tgtgtggtct tctacacaga caagatgaaa
    17701 caattcggca ttaatacctg agagcaggta gagcaaggta aaaggtagta tttgttggcg
    17761 atccccctag agtcttttac atcttcggaa aacaaaaact attttttctt taatttcttt
    17821 ttttactttc tatttttaat ttatatattt atattaaaaa atttaaatta taattatttt
    17881 tatagcacgt gatgctcgcc gcagccgtgt aaccgagcat agcgagcgaa ctggcgagga
    17941 agcaaagaag aactgttctg tcagatagct cttacgctca gcgcaagaag aaatatccac
    18001 cgtgggaaaa actccaggta gaggtacaca cgcggatagc caattcagag taataaactg
    18061 tgataatcaa ccctcatcaa tgatgacgaa ctaacccccg atatcaggtc acatgacgaa
    18121 gggaaagaga aggaaatcaa ctgtgacaaa ctgccctcaa atttggcttc cttaaaaatt
    18181 acagttcaaa aagtatgaga aaatccatgc aggctgaagg aaacagcaaa actgtgacaa
    18241 attaccctca gtaggtcaga acaaatgtga cgaaccaccc tcaaatctgt gacagataac
    18301 cctcagacta tcctgtcgtc atggaagtga tatcgcggaa ggaaaatacg atatgagtcg
    18361 tctggcggcc tttctttttc tcaatgtatg agaggcgcat tggagttctg ctgttgatct
    18421 cattaacaca gacctgcagg aagcggcggc ggaagtcagg catacgctgg taactttgag
    18481 gcagctggta acgctctatg atccagtcga ttttcagaga gacgatgcct gagccatccg
    18541 gcttacgata ctgacacagg gattcgtata aacgcatggc atacggattg gtgatttctt
    18601 ttgtttcact aagccgaaac tgcgtaaacc ggttctgtaa cccgataaag aagggaatga
    18661 gatatgggtt gatatgtaca ctgtaaagcc ctctggatgg actgtgcgca cgtttgataa
    18721 accaaggaaa agattcatag cctttttcat cgccggcatc ctcttcaggg cgataaaaaa
    18781 ccacttcctt ccccgcgaaa ctcttcaatg cctgccgtat atccttactg gcttccgcag
    18841 aggtcaatcc gaatatttca gcatatttag caacatggat ctcgcagata ccgtcatgtt
    18901 cctgtagggt gccatcagat tttctgatct ggtcaacgaa cagatacagc atacgttttt
    18961 gatcccggga gagactatat gccgcctcag tgaggtcgtt tgactggacg attcgcgggc
    19021 tatttttacg tttcttgtga ttgataaccg ctgtttccgc catgacagat ccatgtgaag
    19081 tgtgacaagt ttttagattg tcacactaaa taaaaaagag tcaataagca gggataactt
    19141 tgtgaaaaaa cagcttcttc tgagggcaat ttgtcacagg gttaagggca atttgtcaca
    19201 gacaggactg tcatttgagg gtgatttgtc acactgaaag ggcaatttgt cacaacacct
    19261 tctctagaac cagcatggat aaaggcctac aaggcgctct aaaaaagaag atctaaaaac
    19321 tataaaaaaa ataattataa aaatatcccc gtggataagt ggataacccc aagggaagtt
    19381 ttttcaggca tcgtgtgtaa gcagaatata taagtgctgt tccctggtgc ttcctcgctc
    19441 actcgaccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
    19501 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
    19561 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
    19621 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
    19681 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
    19741 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
    19801 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
    19861 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
    19921 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccggccgcgg
    19981 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    20041 agcccagcgg cgagggcaac caggctcttc aagggttatg cagcggaaga taaaagcggc
    20101 gagggcaacc aggctcttca agggttatgc agcggaagat aaaagcggcg agggcaacca
    20161 ggctcttcaa gggttatgca gcggaagata aaaattcgtt aattatttac ttaatacgaa
    20221 catttaattt aatttatcaa aagttaaata aatttagttt caacgttttt aacgcaaatt
    20281 tccaattacc ataatgattc ttgctttttc actggtactt ttgtcttata gaaaagttta
    20341 acagttatat aactagtttg atctgaggct cctgagcttt tatttagaaa tttattaatt
    20401 tttgtttttt catcgatata aatttcactg acccaaatat caaaaaaatt cacagaaaaa
    20461 ttattgtgta atgaaagaac aggtgagtat aaaattgcta agagattatt acgttcactt
    20521 ggctttaagt taaagagata taaaatatca tcaattgcat agtagatata tttattctga
    20581 aaactgttca aaataaattt agctgttgca gataacttct tttcatattt tatctgaaat
    20641 gtttttgatg taatttcatt tgatttcgga tattgaagag gtttcccttc acttcgtgtc
    20701 ggtgatttac gttttttagt tattgaaaat tcttcattac atctacatct actttcatat
    20761 agaattcgat tcatcgttta gtttatttag cgtttagttt ttctatcagc tttaatatga
    20821 gttctaaaat ttcttgtcga tacaaattct cctagtttat gtccaactaa ttgatccgaa
    20881 ataaaaattg gtacgtgttg ctttccatta tatacagcaa tcgtaaagcc aatcatacta
    20941 ggtaaaatag ttgatgaacg agaccaagtt gtaattgtat cttttttacc agccttattc
    21001 atttgattaa ttttctttaa taaatgatag gcaacaaacg gacttttttt taatgatctt
    21061 ggcatcttga aaattttttt cttaattttt aaactgttca attagtgttt aagaacggcg
    21121 acgcaagata tatgcatcac ttaatttttt tctttttctt gttttcatac ctaaagctgg
    21181 tttaccccat ggtgttaatg gacgtgttcg accaataggt gctcgacctt ctccgccccc
    21241 gtgtggatga tcacatggat tcattacaga tcctcgaact gttggacgtt tacctaacca
    21301 gcgtgttcgt cctgctttac cactctgtac taaaaacgca tcattgttac taacttcacc
    21361 aatcgttgca aaacattctt tgcggactaa acgaatttct tttgatggta aacgtaatgt
    21421 aatataatca ccatctttag ccaaaatttt tgctgatgtt ccaccagctc taacaatttg
    21481 tccgccacga tttggaatta attcgatatt gtgaattgaa cttcctagag gaatttcatt
    21541 caaaggtagg gtgtttccaa tgtttaatgg agaacctgag ccggataata ttcgatcacc
    21601 aacagttaaa ttttttggtt gtaaaatata acgtttctcg ccatcagtat aattaactaa
    21661 agcgatgcgc gcatttctat ttggatcata ttcaatagaa gcgataactc cttcaacacc
    21721 gtatttatta cgtttaaaat cgattaaacg ataccggcgt ttatgaccac caccgcgatg
    21781 acgaacagta ataataccac gattatttcg ccctttattc cgatgatttt ttctaattaa
    21841 actcttttca ggtgtggttt ttgtaagttc atcaaaaacc gataaagcac gatttcttgt
    21901 acctggtgta tatgacttat aaagacgaat actcataaac tagatttttt tattaactat
    21961 ctgtaaataa attgattatg tcaccttcag ataaagttac aattgctttt ttatattgag
    22021 gtttccaccc aacatattta cccacacgtt tttgtttacg tggcaaacgg catgtattaa
    22081 tttttataac ttttacatta aataaatact caatcgcact tttaattgta attttatttg
    22141 aataacggtc tactacaaaa ctatattgat tattttctaa aagccgtgta gctttatctg
    22201 taataatcgg gtatttgata atttgtgcgc tactacgaga aaaatctgaa gaattaatca
    22261 caataactct cctttatatg atttattgct gctgatgtta gaataatttg tcttgctttt
    22321 aatatactta acgtgttcaa acttgacgct gctaataatt caacattttt aagattttgt
    22381 gttgctaatt ttaatacgga tgttttatcg gtaacaacta atagcacttt ttgttctaaa
    22441 ttgatttgac aatcttgaca aatttttaca aaagcttttg tttttggctc cgtcattcca
    22501 gtttctagat tttcaataat taaaatgtta ttttttttat tgaaaattaa agtctgtaaa
    22561 gctagttgac gttccttttt attcagtttt agcgaaattt tttttggttt tgggccaaaa
    22621 ataacaccac cacctttcca taatggcgat ctattagaac ctgcacgggc tttgcctgtt
    22681 cctttttgac gccaaggttt tcgaccacca cccctaactt cacttcgtgt ttttgatgat
    22741 gaagtcgctt gtctttgtga cccaaaatgt cttatcagat ccttatggac taaataattt
    22801 cctgaagttt ctaaaatatt taattttaat tcatgattaa aatttaattg cttaccattt
    22861 aaatctaatg gtgtatatgt tataaatttt tgaatggtca tactctcttt taatatattt
    22921 gttttgtaat ttcagttatt ttatgctgaa ggaacaatac tcaataaatt cccaggtttc
    22981 cctggaacag atcctttgat tactaaaata ttttcttctg aatttatttg aataatttta
    23041 agttttttaa ttgtcgtaat tttgtttcct aattgacctg acattttttt accaggtaat
    23101 acacgaccgg gagttgtacc cataccaata gaaccaggtg ctctatggtt ttttgaacca
    23161 tgtgtcattg gacctcttgt aaaattatga cgtttttgta aacctgaaaa tcctttaccg
    23221 atacttttac cttgaatatt tacaagttgt cctggtgtaa atgaatcaac atttaaaact
    23281 tgtccaattt ggaattctgt ctcttgattt attctgaatt cttttaaata ttttaaaggt
    23341 tgaatatttg atttttgcaa atgacctaat tctggttgag ttaatgcttt acttaaaaca
    23401 ttaccatatc ctatctgaat cgaatcatat ccatcttttg actgattttt cacttgcgtt
    23461 atgacacaag gaccaacttt taaaattgta accggaataa tattaccaga ctcgtcaaaa
    23521 atttgagtca taccaatttt atttcctaat agacctacaa ccacgagatt cctccacatt
    23581 tattctatac tttttaacta tgcttttcaa ttattagtaa ttggataaac taatttgaat
    23641 taatgaatct ttttcattag tttaaaaaat ttcctaaatt ttgtctatat aagttattta
    23701 tacaatatta ttataatagt aaaagttcta cggtttagat aatctatcaa atggatgcct
    23761 aaactattac aatttctata gaaatgttaa aataaaaata aaaagagaaa aaaatgggaa
    23821 aagtagtagg aattgattta ggcacgacaa attcagtagt tgctgcaatt gaaggt
//
