LOCUS       sequence-42068-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TBC1D7
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgactgagga ctctcagaga aactttcgtt
      121 cagtatatta tgagaaagtg gggtttcgtg gagttgaaga aaagaaatca ttagaaattc
      181 tcctaaaaga tgaccgtctg gatactgaga aactttgtac ttttagtcag aggttccctc
      241 tcccgtccat gtaccgtgca ttggtatgga aggtgcttct aggaatcttg cctccacacc
      301 acgagtccca tgccaaggtg atgatgtatc gtaaggagca gtacttggat gtccttcatg
      361 ccctgaaagt cgttcgcttt gttagtgatg ccacacctca ggctgaagtc tatctccgca
      421 tgtatcagct ggagtctggg aagttacctc gaagtccctc ttttccactg gagccagatg
      481 atgaagtgtt tcttgccata gctaaagcca tggaggaaat ggtggaagat agtgtcgact
      541 gttactggat cacccgacgc tttgtgaacc aattaaatac caagtaccgg gattccttgc
      601 cccagttgcc aaaagcgttt gaacaatact tgaatctgga agatggcaga ctgctgactc
      661 atctgaggat gtgttccgcg gcgcccaaac ttccttatga tctctggttc aagaggtgct
      721 ttgcgggatg tttgcctgaa tccagtttac agagggtttg ggataaagtt gtgagtggat
      781 cctgta
//
