LOCUS       sequence-42073-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Mihaylova MM, Vasquez DS, Ravnskjaer K, Denechaud PD, Yu RT, Alvarez
            JG, Downes M, Evans RM, Montminy M, Shaw RJ
  TITLE     Class IIa histone deacetylases are hormone-activated regulators of 
            FOXO and mammalian glucose homeostasis.
  JOURNAL   Cell. 2011 May 13;145(4):607-21.
  PUBMED    21565617
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-05-13"; volume: "145(4)"; pages: "607-21"
COMMENT     Sequence Label: pAd FLAG HDAC5 S259A/S498A
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(121..145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             171..212
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(252..271)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    296..344
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggtgggggcc gagcaggccc aggctgcggc agcccgggaa cacagcccca ggccggcaga
       61 ggagcccatg gagcaggagc ctgccctggg ggattataaa gatgatgatg ataaataaat
      121 acccagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ggtaagccta
      181 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gtttaaacgg
      241 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
      301 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
      361 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
      421 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
      481 caacgtcggg gcggcaggcc ctgccatagc agatccgatt cgacagatca ctgaaatgtg
      541 tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat
      601 ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag
      661 ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc
      721 cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt
      781 gtctggaacg ccgttggaga ctgcagcctc
//
