LOCUS       sequence-420756-       23908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing a 16,121 bp fragment of the P. tricornutum 
            chloroplast genome. The fragment is flanked by SapI recognition 
            sites, allowing it to be released from the cloning vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23908)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 23908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP4
FEATURES             Location/Qualifiers
     source          1..23908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    76..326
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(329..1084)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESMCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      1175..1394
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      1462..1993
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1470..2084
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        16985..17087
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             17088..17747
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(17154..17173)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    17967..17988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18003..18008,18009..18026,18027..18033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18041..18057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18046..18068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18065..18081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18065..18081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             18077..18352
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     misc_feature    18124..18180
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(18182..18201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18183..18201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18208..18225)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18208..18224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18217..18239)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(18432..18451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    18519..18552
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    18570..18968
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     CDS             complement(19901..20872)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(20872..22047)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(22443..23117)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(23118..23265)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    23319..23822
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac acgcggatag
       61 ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga actaaccccc
      121 gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca
      181 aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag
      241 gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg acgaaccacc
      301 ctcaaatctg tgacagataa ccctcatact atcctgtcgt catggaagtg atatcgcgga
      361 aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca
      421 ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag
      481 gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag
      541 agacgatgcc tgagccatcc ggcttacgat actgacacat ggattcgtat aaacgcatgg
      601 catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta
      661 acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg
      721 gactgtgcgc acgttttata aaccaaggaa aagattcata gcctttttca tcgccggcat
      781 cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta
      841 tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga
      901 tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga
      961 acagatacag catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt
     1021 ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg
     1081 ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga
     1141 gtcaataagc atggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag
     1201 ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt cacactgaaa
     1261 gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc
     1321 taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag
     1381 tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg
     1441 ttcccttgtg cttcctcgct cactcgaccg ggagggttcg agaagtgggg gcacccccct
     1501 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     1561 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     1621 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     1681 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     1741 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     1801 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     1861 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     1921 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
     1981 aacgccggcg gccggccgcg gtgtctcgca cactgcttcg acggcgtttc tggcgcgttt
     2041 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccaggctctt cacgccatgt
     2101 tcagcaagta aaatatttaa ttcacaattt actttataaa cttcttcacc taaccagata
     2161 atagttttat gagaaacaac aggtttataa ttgttcaaga agttaacacg acttaaataa
     2221 ttaatagttg aatttggttt gtaattttta tatacatttt ggtgatcata gtaaactgtt
     2281 ccacccacta atgttttaaa cctattagtg attaaggtag ctacttgata atttcttgtt
     2341 gcttgtaaat ataaccgtaa atctttcttt tgaattttta ctaaatattt atcaccttgt
     2401 aaattcaata aataattttt gtgctgaata gaatatctta atttttctaa ctttgaatta
     2461 aaaaatgcat atttattatt tttaatccta attaaacgtt gatataaatt tgatttagta
     2521 ttgataaatt ctacagagcc agaataatga ttaatgattt tagttctaaa gatataactt
     2581 ctacagttta atttatagtc agaataaaaa ttaacaaatg agttaattgg gccattataa
     2641 agttgtccag ataaaatcca caataattta ttattattta gtagattaat ttttttcttt
     2701 aaaacgggca taaaaatttc accacaggta tcgcttaaaa taggttttac ttccgtttta
     2761 atctgtctat ttgtatttaa taattcacca ataacagtat ctttcacaat atattgattg
     2821 tttttaggaa ataaaattgt atttcgtaaa atttcaattt gtactaaatc ttgatcttca
     2881 tcgtctggaa ttaaaatcac agaaccagaa ttttttgtaa ttaaaacgtc ttcaccccga
     2941 tttgttctta ataagacggt ttttaaagtt ttgtaaaatc ttataacacc gttaatagga
     3001 gtagtaattt gttgacgagc ttccgacgta aaaataccgc cggtgtgaaa tgtacgcata
     3061 gttagctgag tacctggctc accaatagat tgacccgcaa gaattccaat tgcttcacca
     3121 atatcgacta aattctcatt tgctaaatcc catccataac agttttgaca aattgctcga
     3181 tataaattac aagttaatgg tgaacgtata taaaatttct tgacattata ttccttaaat
     3241 ttctcaataa gttttggagt gatttgagta cctgccggaa ccaaaatcct atttgttttt
     3301 ggatcatgaa ttggtttgct taaaacacgc cctaaaatct tttgataaat caaatttagt
     3361 gatttcaatt cttcgttact aactgaaaac aagaatgagt gattggtcaa acaatctttt
     3421 tctcgaatta aaacatcttg tccaacatca attaaacgtc tcgttaagta tccagaattg
     3481 gctgttttta atgctgtatc aacaattcct tttctagcac catatccaga catcaaatag
     3541 tcagttattg ttaatccttc cctgaaattt tttttaatcg gtaaattcat tatttcacca
     3601 cttgggtcgg acattaaacc ccgcattcct actaattggc gaacttgaga taaatttcca
     3661 cgagcaccag aaaatgccat aatatagacg gaatttaaag ggtcataatt cttaaaataa
     3721 tatattactt gttctttcaa agactcgctt gttaggctcc aagtatcaat aattttttga
     3781 aacctttcaa cgtcagtgat ttttcctttt agatatattt tttcagcatt tataatttct
     3841 tgattcgctt tttcaagcat caaattttta acaaatggaa tttttaaatc ctcaatacta
     3901 atagaaattc cggcttgact agcatattta aatccaaggt atttcaattc gtcagctaat
     3961 gcacaagctt gcattgaatc ataattagta aaactccaag ctaataattg gcgtaactgt
     4021 ttttttccaa tcaacgtatt ttgatattta taatttttca taaaattgat ttgctatttc
     4081 acgttattat ttataaaaaa tttagggttt tttgaattat ataatttaaa atggcacgtc
     4141 ctgtagttgt ttgtacatat tgaacaatta attgattatc tttagtttta cgaatctgta
     4201 aattttcata aaactcaatt tttgtttcat cgtctaaaat agttgtttta agtaaaattg
     4261 attcgtttat ttcttgttct tccaatctaa tccatattgc gctatgaatc tctaatttac
     4321 tttggctata agctagtatt acatcagcaa gattagcaaa atagtgactt gaacctaata
     4381 atccattaat gttattaaca gttaaataat aacatcctaa taccatatct tgactcggca
     4441 taatgattgg ttctccgttt gccggggata aaaaattata tggtgctaac atcaacatat
     4501 aacattcagc ttgagcttct aaagataaag gaatatgaac agccatttga tcaccatcaa
     4561 aatcagcgtt aaaagctgaa caaactaacg gatgtaattt aattgctcga ccttgaacca
     4621 atataggttc aaaagcttga atacctaatc gatgtaaagt tggtgctcga tttaaaaaaa
     4681 tcgggtgact ttttaaaact ttttctaaaa ccggatcaat aattgattca ttttgttgaa
     4741 ttaaattttt agcaattttc atattacttg ctaaaccttg attaattaat tcacgaataa
     4801 taaaaggttg gaataactca attgccattt cataaggtaa gccacattga tttaatctta
     4861 aacttgggcc tactacaatt actgaacgac ctgagtaatc tactctcttt cctaataaat
     4921 tttgtcgaaa acgaccatgt ttacctttta tgatatcgga taaagatttt aaaggcctat
     4981 tatttgcact taaagcaatt ttaccacgtt ttccattatc aattaacgta tcaacagctt
     5041 cttgtaataa acgtttttcg tttctaatga ttaattgtgg cgcatcaatt tctaaaagtc
     5101 gaagtaatcg attatttctt gtaataatgc gtctataaag ttcatttaaa tcggaagtag
     5161 caaatcgccc gccctctaat tgaatcattg gacgaagcgc aggtggaata actggtaata
     5221 tagtaagaat catccaagct gggcttgatc ctgttccaac caaattttct aaaattcgaa
     5281 ttcgttttat agcttgttca cgaattttat aaatacgctg ttgttcccat tttctaaacc
     5341 atcttgattc atcataaata ggcttttctt tattcaatac tttcgtacaa actaaaataa
     5401 agtaacgcat gcgaaaaatt tctgacttta gatttaattt ttcaagctcc cgtttgatca
     5461 aaggagcacc gattaaaaaa ttaggtgttg ctcgtaataa atatttagga gtactataac
     5521 gacgattttg gggttttgga tatggtttta atggataacc agtataacct aattctcgac
     5581 tccttctata ttgctgtaaa tcccaatgaa tgccataaaa tgaaatttca tcttcggcaa
     5641 tgaaataggc aagagaggct agtttaatac gtttcactcg ttcatcccat aaatcaacaa
     5701 ctgttgaggt tgttaattta agaccgtcac attttttaca attttctgaa tgcgttgtgt
     5761 aagttgtatc cagctttttt tcaaattctt ctacttctaa taatagagcc atgaaatttg
     5821 ggcgactatt tatataccaa acatgagtga caggataaat taaattaata taccccattc
     5881 tatgacgtcg gactcttgat tctgttagtt caacgccaca tctttcacaa attaaacctt
     5941 catatctcac acgcttatat ttaccacaag cacattctaa acttttactg ggcccaaaaa
     6001 tacgttcaca aaataatcca tccatttcag gcttaaatgt acgataatta attgtctctg
     6061 atttttgaac ttctcctacg aattggccat ttggtaattt tcgatttgcc cattgtaaaa
     6121 tccgaaatgg agatgccagt ttaattttta tatagtcaaa ttcttttgtc gattctacca
     6181 ttagtaatat taaaatgaaa tatcgtttat atttgaagta ggggaaaaag ttttggataa
     6241 agcattatat ttttcgatta aattgacttc aacttcataa cgttttgtgg aattaaatcg
     6301 attaatttta tatgtactca tatctaatcc aatagagcgt aattcatgca ataaaacttt
     6361 aaatgattca ggaattccga atttaggaat ttgttgacct ttaacaatag catttaaagt
     6421 ttcatttcga ccttgcatat catctgattt tattgttaat aattctttca gtgtaaaagc
     6481 agctccaaaa ccttctaaag cccaaacttc catttcccca aaccgttgac ctccatgctg
     6541 ggctttacca cgaagtggtt gttgtgtaat taatgaatat ggacccgttg ctctagcatg
     6601 cattttatca tctactaaat gaataagttt taacatataa gcatttccaa ctgtaacagg
     6661 attttcaaac tcttttccgg ttcgaccatc tattaacacc attttaccag gtgcataagg
     6721 attaaatagc caactttcat ttttagcaat ggacgcttgc cgtaattttt tattaattag
     6781 aatgcgggaa atttctaacc catacatttc atcaaatggc aaaattttaa atctcgcatt
     6841 taatttgtca cccgctagtc ctaacagaca ttcatacaat tgaccaacat tcatacgcga
     6901 tggtacacct aatggattta ataaaatgtc taatggtgtg ccatcgggta aaaatggcat
     6961 atcttggcgc gataaaatac gcgaaattat acctttgtta ccatggcgtc cagcaatttt
     7021 atctccgact tgaatttttc taatttgagc aataaaaaca taaatttttt caaatttata
     7081 tgttaatttc gtacgacgat taaatgttac cgtttcaata acccgaccat attctccatc
     7141 tgacattcta taagagttat cttttactcc tttagctttt gcaccaaata ttgctcgtag
     7201 taattttgat tcaggtaact gttcagatgt gttatttgaa ataactttac caactaaaat
     7261 atcacccggt cgaacaaatg tacctacagt aactattcca tcatcattta aatgcctaat
     7321 ttctcgaggg tttaaatttg ggatattttt tgttgttcgt tccaatgtat ctgaatttcg
     7381 atcaatctca attttatatc tttcaatatg aatagacgta aatacgtcgt cgtacactaa
     7441 acgttcatta attaagatag catcttcaaa gttataacct tgccaaggca tataaccaat
     7501 taatacattt tgacctagag ataattcact gcttgtaatc gctggtccgt cagttaaaat
     7561 ttggccagat tttacttgtt cacccttcca aacaattggg cgatgattaa tacaagtttg
     7621 ttgatttgag cgcaaatatt tttgtaattt ataagttaat cttttgccag ttttatcatt
     7681 aactactatt ttattggctg ttactgaact aacgacaccg tttgaataag aatttaaagt
     7741 catacccgaa tcaattgcaa tttgattttc taaacctgtc ccgacaattg gtttttgagg
     7801 caacaataag ggaactgatt gccgttgcat attcgaaccc attaaggctc tatttgcatc
     7861 atcgtgttca aaaaatggaa tcaatgaggc cgctactgaa acgacctgaa ttgtcgatat
     7921 tgcaataaaa tctacttcag aaggtgtgac attaacaaaa tcttgtttgt aacgaactgg
     7981 aataacattc tttgttaaat agttatcctt attagttgca atatcagcag gagcaatttt
     8041 gtaaaaatct tcaatatcgg ccgttaaata gacaggctga cctgttttga ccactttacc
     8101 gttaattact cgccagaatg gtgtttctaa aaatcctgac ttatttacac gcgcacatgt
     8161 cgttaatgaa gcaattaatc caacgttttg cccttcgggt gtttcaatag gacaaattcg
     8221 accataatga cttgggtgaa tatcacgcac tgcaaaacta attcgatctc tatcgaagcc
     8281 gcctggccct agtccactta cacgtcggcg atgtgtaagt gacgataatg gatttgtttg
     8341 gtccagatat tgtgataact gacttgaacc aaaaaattct cgaatacttg cattaactat
     8401 gttaaagctt agtaactgac ccgaatcaat tttattagtt tggttacgta attttctacc
     8461 taaacgttga aaaccaattc taaaaagatt ttgtaataat tctcctacag atcgaactct
     8521 tcggtttttt aaatgatcaa tatcatcttg aactgtttta gaaatagtta aattaattag
     8581 tttatcagta attgcaaaaa tgtcttcata cgttattgtt tgcaatcgtt cactcatttt
     8641 taaatttaaa cgactattaa tttttaatcg cccaactcga cctaaacgat aataagtagg
     8701 atcaaaaatc cttgaaaatt cagaaatatt tttaaaataa cttaaaccta aattaaacct
     8761 tgataaaggc tcagagagac gttttgaatt aattaatagc ggtttattga aataaaagaa
     8821 atcagcgtat tctaaatttt gataaatttc taaatcagtt agacccattt cttttaataa
     8881 ataaagaact ggcttttgat ttatcttgtc caactgaata atgacttcat cttcttgact
     8941 tttaagagga tacttataag aattaatttt tgtattgcgt tgaaaaccga tacgaatcca
     9001 cgatccgtat tctggaatta ctgttgctgt ataaagatcc ttttcatcgt attttttatt
     9061 ataatttgtt ttaatttcat gatcttttcg ataaagaaga aattgggttt tagtttttaa
     9121 atatttatga cgatcagact tataggttaa tttgtttttc ccaaagacat atttcaattg
     9181 ttcttttaga cttattgaga aataaattgc tggttttaaa ttttgtaact gtgttaattc
     9241 ttgaaaactt ttcaatgtaa aggtagcttt actgatttgt tttttcaaaa ataaaaaatt
     9301 actcatctca gttagccatt ctcggggctc ttttgtaatt aaaaacaaat ttgaatttaa
     9361 ttccatttga gccaatgttt gagaattaat gattaatttt ttataaatca tggacaaatt
     9421 tatgatttgt ttatccgaga gatttaaaat agcattcgac tttgtgaatt ttgatagtaa
     9481 aatcgtttta tcttcattaa attttgttgt taaactggat tgcaaaattt catacttcat
     9541 caatgatgta agtaaaaaat taaaatattt aattaaataa atttgttttt catcttgaaa
     9601 ttcaaaagat ttattcttca gtttcaacca ttttaaaaat aattgaatta cgcgttgttt
     9661 tgattttgat gaaagtaatt ttgaggccac ccgatatagt ttaaaagatt gcaaaaaata
     9721 aaatgttgaa ttttgttgtt tctgttttaa gtacttaatt gagtaataat aaatggagtt
     9781 actttgccag tgtgggatta tttttttttt ctttttaact ggatcgtaaa ttgatgttgg
     9841 tagaggaaag aataaatcaa actcagagat aaaggcttca ccggatggaa taaatgatct
     9901 tagtttatta atatcagtag acaatttttt tttaaattga tttctagtct tttggttttt
     9961 atttttttca aaataaacgc caggacttcg aataatttga ctgacaataa cgcgttcaca
    10021 accatttatt ataaaagtcg cgtctgtagt cataagtggc aacgtaataa tcggaaattg
    10081 attatgatac cttatactat ttactacttt atttctgact tcaattggaa ttactaattg
    10141 agctcgatag tttccgctat attttcgtgc aattagtaaa ctgtatgaag gtttaattaa
    10201 gctatattct tctccaaaca taacatattc agtattttga ctgaagtctt gaattcggga
    10261 aaataaagca agttcttctg taagcccttg agtaataaac caacaaaaac ttattctttg
    10321 catggctaaa aaatctggga gagcattagt ataattcata ttttttttca taatttttat
    10381 tagtgaaaca acgaatagaa tatatttaga ttcgtcagtg actaaccgta aattcttaaa
    10441 aataaatgtt aagaaaattt aaaattttct taacgcgata aagtatcaac gaatctaact
    10501 tatgatattt ttaaagacga ggctaatttt gccttttttc gagccgccgc atttttatga
    10561 aatacatttt tttttgttcc cttatcaatt agactataaa cagagcttaa aattttttgt
    10621 aatttttctt ttccttctgg acttttcgaa gttttgtaaa tttctaaatt ttttaagaat
    10681 actttaatta atgtacgaac agaacttttg taaaaacgat tttgtaaacg attacgttca
    10741 tttacttgaa tacgtttttg cgcggattta ttattagcca tattattatt aattaattaa
    10801 tagttgtttt ataaataagg ggttttgact aatataatat atttgacaaa aaattaaaag
    10861 tttttatgat aaaaattttt aattctaata ataactatta acccttgata agatagaaaa
    10921 ttttaggcaa gatagtaagt aaaattatta gtcaacaatt ttatggcaaa aaataaaggt
    10981 actcgaatct tgattacttt agagtgtact gaatgtcgtt cgaatttaga gaagcgttca
    11041 cctggtgttt cccgttattc gacgcagaaa aatcgtcgaa ataatccaga aagattagaa
    11101 ttaaaaaagt attgttcgca ttgtaataaa acaactctac acaaagagat taaataaatt
    11161 atggtatcaa aaaaacaaaa attgtctcca attagcctaa atcaaaatat tgactacaaa
    11221 gatatcgatt tattaacttt atttgtcacc gaacaaggaa aaattctccc acgtcgtgcc
    11281 actggtgtga cggttcaaca acaacgaaga ttagcgaaag caattaagcg tgctcgtgta
    11341 ttatcattat ttccttttgt agcatctaat tctatttaaa attatttaga atcactatat
    11401 tttaacatgt agtgattcta aatgtttaat ttataaaatt tataaggaaa gttctatggg
    11461 aaattttatt gataaaacat tcacagtaat agcagatatt cttttaaaag ttttgccagc
    11521 tagtaaacaa gaaaaagaag ccttttctta ttatcgtgca ggtatgagtg ctcagtcaaa
    11581 aggtcgctat gctgaagctt tacaaaacta ttatgaagcc ctacaagttg aagaagatcc
    11641 atatgatcgt agttatacgt tatacaatat tggtctcatt tacggtaata caggaaaata
    11701 tacacaagct ttagagtttt atcatcaagc gttatcctta aacgcgaatc ttcctcaagc
    11761 tttaaataac atcgcagtta tatatcattc acaagcttta cgggctcaga gtctagaaga
    11821 agatgaatac atcgaattat caaaagaact ctttgataaa gccgcggaat attggattca
    11881 agctcttaaa ctagcaccag ataattatcc aggagcacga aattggttaa aagtaacagg
    11941 tagattaagt gataataact aattaataat cgaatacgct tttcaaaaaa tcttataagt
    12001 atcaagaatt caaattattt tgttgtacaa tgtttaataa ttattaaact tgaagtttga
    12061 taaaatttaa actgataaat aaaaccgtga aagtatattt attctcaagt attttaaata
    12121 tgttaaatca aaaatggtaa aaactaattt aaacaaaggt tacgttactc aaattattgg
    12181 tcctgtatta gacattaaat tttcagaagg aaatttacca ccgatctata gtgcaatcaa
    12241 aattatcttg gatgataata ccgaaacaat tgttgaagta caacaattat taggtgataa
    12301 taaggtacgt gcagtgtcta tgcgttcaac agatggttta aaacgtggtg tagaagcaat
    12361 tgatttaggc actccaatta atgttccagt cggtactcca acgttaggtc gaatttttaa
    12421 tgtaattggt gagcctgtcg atgagcaagg cgaagttaaa tatgatgaaa ctttaccaat
    12481 tcatcgtgat gctccagcat tcactgagtt agaaacaaaa ccatcaattt ttgaaactgg
    12541 tattaaagta gttgatttat tagcaccata tcgacgtggt ggcaaaattg gtttatttgg
    12601 tggagctggc gtaggtaaaa cggtattaat tatggagtta attaataata ttgcaaaagc
    12661 tcatggtggt gtatctgttt tcggtggtgt cggtgaacgg acgcgcgaag gaaatgattt
    12721 atacgaagaa atgaaagaat ctggtgtaat taatgaaaaa aactttgcgg aatctaaagt
    12781 agctttagtc tatggtcaaa tgaatgaacc accaggggca cgtatgcgtg ttggtttaac
    12841 agctttaaca atggctgagt attttcgcga tgtaaataag caagatgtat tattatttat
    12901 tgataacatt ttccgcttta ctcaagctgg ttcagaagta tcagcattat taggtcgtat
    12961 gccatcagcg gtaggctatc aaccaacatt agctactgaa atgggtgcat tgcaagagcg
    13021 aattacatca acaacgcaag gttcgattac atcaattcaa gctgtatacg taccagcaga
    13081 tgatttaaca gatcctgctc cagcgacaac attcgcacat ttagatgcga caactgtgtt
    13141 atcccgcggt ttagcagcga aaggtattta tcctgctgta gatccgttag attcaacttc
    13201 aacgatgtta caagtgggta ttgttacaga agaacattat gctacagcag aaaacgtaaa
    13261 agaaacatta caacgctata aagaattaca agacattatt gcaattttag gtatcgatga
    13321 attatcggaa gaagatcgtt taactgttgc acgtgcacgt aaagtagaac gctttttatc
    13381 tcaaccattt ttcgttgctg agatttttac tggttcacca ggtgaatacg ttagtttaga
    13441 ggatacaatt aagggtttct taatggtatt aaacggtgaa ttagatgatt taccagaaca
    13501 agcgttttat cttgtaggta atattgatca agcaattgca aaagcagaaa ctctaaaata
    13561 aggaatacga catatggtta tgaacattcg cgttcttacc cctgacagag taatttgttc
    13621 tacaacagcg gatgaagttg ttttacctgg tttaacaggt caagtcgggg tattagatgg
    13681 tcatgcagca ttaataacag ctttagagac aggattatta agaattaaat taaacgacaa
    13741 atggacacca attattttat gtggtggttt agctgaaatt gatcgagatc gtgttactgt
    13801 tttggtgaat gatgttgaag aacttacgga tatcggatta agtgaagcga cacaagaact
    13861 agaaaaagct actttagccg ttgaaaatgc gaagacgagt aaagaaagat tagatgcttc
    13921 agttgaatta aaaaaagcaa ctgctcgtct tgaagctgtg aattatttat cataaatatt
    13981 aataatcttc taagaactct ttttgtctaa ataatctgtt taacgattat ttagacaatt
    14041 aaatttaaca aactatcacc aataaatttt tattacatga cgactaattc taattttaat
    14101 tttacgaata atacaacagt aacattggaa ttaccttttt cagaacatat tgaagaatta
    14161 agacaacgaa tttttcttgt attttggata attctattat taacgtctgt atcatttatt
    14221 gaagtcaaaa gtttagttaa aattttagaa atgccaatta caaatgtaaa attctttcaa
    14281 gtagctcctg gcgaatattt tatttcaact attaaaattt cattttacac agggttactt
    14341 ttttcaagtc catttgtaat aggtcaattg attctatttt tactaccagg cttaacaaaa
    14401 aaggaaacaa aaattgtttt acctttgcta ttaagttcat tagttttatt tgcattagga
    14461 ttagcttttt catattacac gttaattccg gctgctttaa actttttctt aaactacagt
    14521 gaagaagttt tagaaccatt ctggtctttt gatcaatatt ttgaattcat tttagtttta
    14581 ttttacagca ctggcttagc atttcagata ccgattattc aaattttagt tgggttatta
    14641 aatattataa cagctaaaca aatgttgggt gcgtggcggt atataatact agtgtcgaca
    14701 attcttgggg caattttaac gccctcgact gatcctttta cacagttgtt gttatcttta
    14761 gcaatactgt tattatattt atcaggatta ggtatcttgt ttttaataaa gaactaattt
    14821 atataataaa atatccatac ttaaaaaata tttaaactat ggcaactaac aagtttttta
    14881 aaagtctttt atttgcttta acaattgcta taaattcatt tggtttttgc attcaagacg
    14941 cagtagcata tccagttttt gcacaacaaa actattcaaa tccacgtgct gcaaatggta
    15001 aattagcctg tgcaaattgc catttaaatc aaaaagcaat tgaaattgaa gcaccccagg
    15061 cagttcttcc aaattcgatt tttgaagtcg aaattaaagt tccctacgat actactaaac
    15121 aacaactcgg tgcaaatgga aaaaaagctg atttaaacgt tggtggaatt ttaatgctac
    15181 ctgaaggttt taaattagca ccgaaaaatc aaattccagc tgaagtaaaa gaaaaaaata
    15241 aaggtgtttt tatttcaccg tatagtagtg aatttgataa tatcttagta gttggaccaa
    15301 ttgcaggaaa gactcatcaa gagttaattt tccctgttat ggctcctgat ccagagaaaa
    15361 attcagatat aaaatattta acgtatccat tctacgctgg tggtaaccgt ggccgtggtc
    15421 aagtatatcc cacaggtgaa aaaagtaatg taaatgtatt tggtgcaaat caaagcggtc
    15481 aaataacaga gattacggta actgaaaagg gagagtctac tatcttaatt ttaaattcaa
    15541 acggtaaaca aacatcacaa gttcttccgg ctggattaat tttaagtata aaacaaggcc
    15601 aagtagtaaa agctgaccaa cctttaaata ttgatccaaa tgttggtggt tttggccaag
    15661 aagaatctga aattgtttta caaaatccga tccgtattta tggatatctt gcattttgtt
    15721 ttagtgtatt aataacacaa attatgttag tattaaagaa aaaacaattt gaaaaagtgc
    15781 aagctgcaga attaaacttc taaaggtatt tcgataatta aggaatagag caaattaatt
    15841 cgccttattc cttaattatc ttgaacttat gtagataagt taatataatt catcttcttg
    15901 atgtactgaa attgtacaat ctgatttcgg gtatgcaata caagttaata caaaaccagc
    15961 ttcaacctga tcatcatcta aaaatgtttg ttctgattga tcgatttcac cagctgttac
    16021 tttaccagca caagtagagc atgcacctgc gcgacatgaa taaggtaatt caataccttg
    16081 ctcctcagca gcatctaaaa caaatacatc atcattacaa tcatgaagag cgggcttcgc
    16141 cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccac atcatggttc
    16201 tgtgttcatt aggttgttct gtccattgct gacataatcc gctccacttc aacgtaacac
    16261 cgcacgaaga tttctattgt tcctgaaggc atattcaaat cgttttcgtt accgcttgca
    16321 ggcatcatga cagaacacta cttcctataa acgctacaca ggctcctgag attaataatg
    16381 cggatctcta cgataatggg agattttccc gactgtttcg ttcgcttctc agtggataac
    16441 agccagcttc tctgtttaac agacaaaaac agcatatcca ctcagttcca catttccata
    16501 taaaggccaa ggcatttatt ctcaggataa ttgtttcagc atcgcaaccg catcagactc
    16561 cggcatcgca aactgcaccc ggtgccgggc agccacatcc agcgcaaaaa ccttcgtgta
    16621 gacttccgtt gaactgatgg acttatgtcc catcaggctt tgcagaactt tcagcggtat
    16681 accggcatac agcatgtgca tcgcatagga atggcggaac gtatgtggtg tgaccggaac
    16741 agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc tccccaatcc aggtcctgac
    16801 cgttctgtcc gtcacttccc agatccgcgc tttctctgtc cttcctgtgc gacggttacg
    16861 ccgctccatg agcttatcgc gaataaatac ctgtgacgga agatcacttc gcagaataaa
    16921 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
    16981 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
    17041 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
    17101 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
    17161 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
    17221 taaagacagt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
    17281 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
    17341 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
    17401 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
    17461 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
    17521 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
    17581 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
    17641 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
    17701 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
    17761 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
    17821 atggcagaaa ttcgatgata agctgtcaaa catgagaatt ggtcgacggc ccgggcggcc
    17881 gcaaggggtt cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactag
    17941 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    18001 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    18061 cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaga ctatagaata
    18121 ctcaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
    18181 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
    18241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    18301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagct gaatggcgaa
    18361 tggcgcctga tgcggtattt tctccttacg catctgtgtg gtatttcaca ccgcatatgg
    18421 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
    18481 acacccgctg acgcgaaccc cttgcggccg catcgaatat aacttcgtat aatgtatgct
    18541 atacgaagtt attagcgatg agctcggact tccattgttc attccacgga caaaaacaga
    18601 gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgtttc ctttcttttc
    18661 agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac
    18721 cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc
    18781 accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac gtgcgtggag
    18841 gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat tgatctgcat
    18901 caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat acggggcaac
    18961 ctcatgtccg agctcgcgag ctcgtcgaca gcgacacact tgcatcggat gcagcccggt
    19021 taacgtgccg gcacggcctg ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg
    19081 ttgtggataa gcaggacaca gcagcaatcc acagcaggca tacaaccgca caccgaggtt
    19141 actccgttct acaggttacg acgacatgtc aatacttgcc cttgacaggc attgatggaa
    19201 tcgtagtctc acgctgatag tctgatcgac aatacaagtg ggaccgtggt cccagaccga
    19261 taatcagacc gacaacacga gtgggatcgt ggtcccagac taataatcag accgacgata
    19321 cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtt
    19381 ccagactaat aatcagaccg acgatacgag tgggcgtggt cccagactaa taatcagacc
    19441 gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac
    19501 cgtggttcca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
    19561 aatcagaccg acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac
    19621 gagtgggacc gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc
    19681 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac
    19741 cgacgatacg agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa
    19801 cagtgggccc agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat
    19861 taagtaaaga cagataaacg tagactaaaa cgaggtcgca tcagggtgct ggcttttcaa
    19921 gttccttaag aatggcctca attttctcta tacactcagt tggaacacga gacctgtcca
    19981 ggttaagcac cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc
    20041 gtgagcttaa actagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga
    20101 taacttcttc agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct
    20161 gctgcttaag taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg
    20221 cagatagttc accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt
    20281 tgatacagcg ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat
    20341 ttccagcaaa ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg
    20401 ttgggcgata atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc
    20461 caaccagaac acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat
    20521 cggcaatttc tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca
    20581 gtagaaaaga agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt
    20641 cattacctga ccatacccga gaggtcttct caacactatc accccggagc acttcaagag
    20701 taaacttcac atcccgacca catacaggca aagtaatggc attaccgcga gccattactc
    20761 ctacgcgcgc aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt
    20821 caaccggttg agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat
    20881 ctcccagcgt ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga
    20941 aagagcattt ctccaggcac cagttgatga gcgttgatca atggcctgtt caaaaacagt
    21001 tctcatccgg atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac
    21061 catgcttccc caggcatccc gaatttgctc ctccatccac ggggactgag agccattgct
    21121 attgctgtat ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt
    21181 cttgagcaga tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc
    21241 agcaggcgtg ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc
    21301 caggttaggc gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc
    21361 cagtcggagc atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa
    21421 ctcagtttca atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca
    21481 agtgggcttt attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt
    21541 gtcttctgca tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg
    21601 ggggtcgtta ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag
    21661 atgaacagaa actgaggttt tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg
    21721 tggaaatacg tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat
    21781 ttgttcaatt gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg
    21841 cggtagtcgc cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc
    21901 cgctgcttca cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt
    21961 aaactgtgca atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag
    22021 tgtttccatg agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa
    22081 tcttgcaatt tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt
    22141 gtttaaaata agagcaacac tacaaaagga gataagaagc gcacatacct cagtcactta
    22201 ttatcactag cgtcaagtcc agactcctgt gtaaaatgat ttataaggga ttttgccgat
    22261 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    22321 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    22381 tcacaccgca tagatcggca agtgcacaaa caatacttaa ataaatacta ctcagtaata
    22441 acctatttct tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt
    22501 gtctccacac ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca
    22561 acattttctg gcgtcagtcc accagctaac ataaaatgta agctttcggg gctctcttgc
    22621 cttccaaccc agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct
    22681 gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg
    22741 cagtcttttg gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct
    22801 tgccacgact catctccatg cagttggacg atatcaatgc cgtaatcatt gaccagagcc
    22861 aaaacatcct ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa
    22921 ctatttttat atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt
    22981 ctctttctat tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat
    23041 tctgcggcct ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg
    23101 aaattaataa cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg
    23161 ctcaatagtc accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaatta
    23221 attcttaatc ggcaaaaaaa gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct
    23281 atttttcaat aaagaatatc ttccactact gccatctgga tcgcttgcct gtaacttaca
    23341 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt
    23401 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga
    23461 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata
    23521 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg
    23581 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat
    23641 taatacctga gagcaggtag agcaaggtaa aaggtagtat ttgttggcga tccccctaga
    23701 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct
    23761 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg
    23821 atgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa gcaaagaaga
    23881 actgttctgt cagatagctc ttacgctc
//
