LOCUS       sequence-420757-       13068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Preparatory plasmid for operon integration into the genome using 
            CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13068)
  TITLE     Harnessing xylitol utilization in Escherichia coli for 
            antibiotic-free plasmid and recombinant protein production.
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: INT_GFPsp
FEATURES             Location/Qualifiers
     source          1..13068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(345..363)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      359..406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(429..446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(429..445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(438..460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(592..1542)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1590..1812
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(2464..3279)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2464..3279)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3187..3206
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3271..3290
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3702..3719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3730..3764
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             4142..4164
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(4172..4174,4175..4888)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4172..4174,4175..4885)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4214..4235)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4475..4494)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4822..4843
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             11967..11978
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagccaacg aaagtttgca gaaaaaattg tatgcattct ctcagggaaa catgcgttcg
       61 ttgagaaacc ttatttatca agcatctatc gaagcaattg ataatcagca tgagacgata
      121 accgaagaag atttcgtttt tgcatcgaag ttgacatcgg gcgataaacc caactcatgg
      181 aaaaatcctt ttgaggaggg tgttgaggta acagaagata tgttacgacc gccaccaaaa
      241 gatattggtt gggaggacta tttgagacat tcaaccccga gagtgagtaa accaggtaga
      301 aataaaaact ttttcgaata acctaggctg ctgcgctgct gccaccgctg agcaataact
      361 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
      421 atttgttcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      481 cttaatcgcc ttgcggacag ttttcccttt gatatgtaac ggtgaacagt tgttctactt
      541 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
      601 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
      661 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
      721 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
      781 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
      841 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
      901 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
      961 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     1021 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     1081 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     1141 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     1201 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     1261 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     1321 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgaacgta
     1381 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     1441 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     1501 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     1561 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     1621 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     1681 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     1741 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     1801 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     1861 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     1921 ctcggttgcg gccgcaatcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg
     1981 cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg
     2041 aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata
     2101 atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt
     2161 gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt
     2221 cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc
     2281 atcaacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2401 ctaaagtata tatgagtaaa cttggtctga cagttacgtt tccaaccaat taaccaattc
     2461 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     2521 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     2581 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     2641 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     2701 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     2761 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     2821 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     2881 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2941 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     3001 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3061 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3121 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     3181 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     3241 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     3301 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     3361 tcagagattt tgagacacaa cgtggctttc cctgcagggt ttgcagtcag agtagaatag
     3421 aagtatcaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt
     3481 attttaactt gctatgctgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     3541 caacagtttt tatgcatgcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3601 cattaatgca gctggcacga caggtttccc gactggtgga taaccgtatt accgcctttg
     3661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3721 acacaagctt tgacagctag ctcagtccta ggtataatac tagcgtcgac gtggagatat
     3781 accatgggtg aactgccgag taggtagctg ataacgagac cccgcagcag ggaaataatt
     3841 cccgccaaat agctttttat cacgcaaata atttgtggtg atctacactg atactctgtt
     3901 gcattattcg cctgaaacca caatattcag gcgttttttc gctatctttg acaaaaaata
     3961 tcaactttct cgatttgctc tcagccctta tatcacggga aattccggcg atttgctcgc
     4021 atcaatattc atgccacatt tgccatcagg ggttgcctca gattctcagt atgttagggt
     4081 agaaaaaagt gactatttcc attgggtaat atatcgacat agacaaataa atagaaataa
     4141 ttttgtttaa ctttaagaag gagatataca tatgagcaag ggcgaggagc tgttcaccgg
     4201 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4261 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4321 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4381 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4441 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4501 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4561 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4621 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4681 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4741 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4801 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4861 tctcggcatg gacgagctgt acaagtgatg ggctgaaggg tttaagcgaa gacgaacaat
     4921 aaggcctccc aaatcggggg gcctttttta ttgataacaa aaaggcaaca ctatgacatc
     4981 ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
     5041 gttactggca gagcggcgcg aaggtctcgt gaactgccga gtaggtagct gataacggat
     5101 ccgaattcga gcgaaggaga tatacatatg tttttgcaaa gacctaaacc ttacagcgat
     5161 gaaagtttag aaagtttctt tatccgagtg gctaacaaaa atggctacgg tgatgtccat
     5221 cgcttcctag aagccactaa acgattcctt caagacattg accataatgg ctatcaaacc
     5281 tttccgactg atataactcg gataaaccca tactcagcta aaaacagttc cagcgcacga
     5341 actgcgtcat tcctgaaact tgcacaattg acatttaatg aaccgccaga gctacttggg
     5401 ttggcaatta acagaacaaa catgaaatac tcgccgtcaa ctagcgcggt tgttagaggt
     5461 gcagaagtct ttcctcgcag tttactacgg acgcactcca tcccctgctg tcctttgtgt
     5521 ctgcgagaaa atggctacgc ctcctacctt tggcactttc aggggtacga atactgccac
     5581 agccataacg tacctttaat taccacttgt agctgtggta aggagtttga ctaccgagta
     5641 tctgggttaa agggcatttg ctgcaaatgc aaggagccta tcaccttaac cagcagggag
     5701 aacggtcatg aggcagcgtg tactgtttca aactggcttg ctggccatga atctaaacct
     5761 ctgccaaatc ttcctaaaag ctaccgatgg ggtttagttc attggtggat gggtattaaa
     5821 gatagcgagt tcgatcactt ttcgttcgtt caatttttct caaactggcc aaggtcattc
     5881 cactcgataa tcgaagatga agtagagttc aaccttgagc atgctgttgt cagcacgtct
     5941 gaattacgac taaaagatct tcttggtcga ttgtttttcg gttcaattcg gttacctgag
     6001 cggaatcttc aacacaatat catccttggt gagcttctct gctatttaga aaatcgttta
     6061 tggcaagaca agggattaat cgccaacctc aaaatgaacg cgttagaggc gactgtaatg
     6121 ttaaattgta gcctcgatca gattgcatca atggttgaac aacgcatctt gaagccaaat
     6181 cgaaaaagca agcccaacag ccctcttgat gttaccgatt atctatttca tttcggcgat
     6241 attttctgtc tttggttagc tgagttccaa agcgatgagt ttaaccgttc gttttatgtg
     6301 tcgaggtggt aaatgcaaac tctgaaagaa ctaatcgcat ccaatcctga cgacttaaca
     6361 actgaactta agagagcatt tcgtccactc acaccgcata ttgcaattga tggtaatgaa
     6421 cttgacgcac tgacgatatt agtcaattta accgataaga ctgatgatca gaaagacctg
     6481 ctcgatcgag ccaaatgcaa gcaaaaactt cgagatgaaa aatggtgggc tagctgcata
     6541 aattgcgtta attacagaca aagccataac ccaaaattcc cggatatacg ttctgaaggc
     6601 gtgattcgaa cccaagccct gggtgaatta ccgagtttcc tactctcgtc ctcaaaaatc
     6661 ccaccatacc attggtcata tagccatgat tcaaaatacg tcaacaaaag cgcattcctc
     6721 accaatgagt tttgttggga tggtgagatc tcatgtttgg gtgagcttct taaagatgca
     6781 gatcacccac tttggaatac tttgaaaaag ttaggttgtt ctcaaaaaac ttgcaaagca
     6841 atggcaaaac aactagctga tattactctc acgactatca atgtcacact cgcaccaaat
     6901 tacctgactc aaatatctct ccccgatagt gatacatcct acatttcact ctctcctgtt
     6961 gcatcgctat cgatgcaaag ccactttcat cagaggctcc aagatgaaaa tcggcatagc
     7021 gcgataacgc ggtttagccg aactaccaac atgggggtca ctgcgatgac atgtggcggc
     7081 gcatttagaa tgttaaagtc tggcgctaag ttttccagcc cccctcatca ccgattaaac
     7141 agtaaacgaa gttggttgac gtcagagcat gttcagtcat taaaacagta ccagcgcctc
     7201 aacaaaagcc tcatacctga aaactctcgg attgcactcc gtagaaaata caaaatcgag
     7261 cttcaaaata tggtcagatc ttggtttgca atgcaagacc atacccttga ttcgaacata
     7321 cttatccaac atttgaatca tgacctatct tacttaggag ccacaaaacg ttttgcatac
     7381 gatccagcga tgaccaagct ctttactgag cttttgaaac gagagttatc aaattcaatc
     7441 aataatggtg agcaacacac taatggatcg tttttagtcc taccgaatat cagggtttgt
     7501 ggcgcaacag ctttaagctc cccggtaacg gtggggattc catcacttac agctttcttt
     7561 ggcttcgttc acgcatttga acggaatata aatcgcacca cctcatcgtt tcgtgttgaa
     7621 tcctttgcga tatgcgtcca tcaactacat gtcgagaagc gaggtttgac agcagagttt
     7681 gtggaaaaag gcgacgggac tatatccgct cccgcgaccc gggatgactg gcagtgtgat
     7741 gtcgtattta gccttatttt gaacaccaac tttgctcaac atattgacca agatacgtta
     7801 gttacatcac taccaaagcg attggctcgg ggttcagcaa aaattgcgat tgatgacttt
     7861 aaacatatca actcattctc gacattagaa acagcgatcg aatctctgcc aatagaagct
     7921 ggtaggtggt tatcacttta cgcacagtca aacaataatc taagtgatct attagcagcc
     7981 atgacagagg accatcagct catggcaagc tgcgtcggtt accacttgtt agaagagccc
     8041 aaagataaac caaactccct cagaggttac aaacacgcta tcgccgagtg catcattgga
     8101 ctcattaact caatcacctt tagctcagag actgatccca acacaatctt ttggtcgcta
     8161 aagaactatc aaaactacct agtggtacag ccaaggagta tcaacgatga aactaccgac
     8221 aaatctagcc tatgagcgct ctatcgaccc atcagatgtc tgtttttttg tcgtctggcc
     8281 cgatgataga aaaacacctt taacctacaa ttctcgtact ctgctcgggc aaatggaagc
     8341 ggcatcatta gcctatgatg tctcaggtca accaataaaa agtgccaccg ctgaggcgtt
     8401 agctcaaggg aaccctcatc aagttgattt ctgccacgtt ccatacggtg cgagtcatat
     8461 tgaatgcagt ttctccgtct cgttttcttc tgaactacgt caaccatata agtgtaactc
     8521 aagcaaagtt aaacaaacgc tagtgcaatt agtcgagctc tacgaaacga aaatcggctg
     8581 gactgagcta gcaacccgat atttgatgaa catttgcaac ggtaaatggc tgtggaaaaa
     8641 tacccgtaaa gcctattgct ggaacattgt acttacacct tggccttgga acggggaaaa
     8701 ggttggattt gaagatattc gtactaacta cacctcacgg caagacttta aaaataataa
     8761 aaattggtct gctatagttg aaatgatcaa aaccgcattt tctagtactg atgggctggc
     8821 gatatttgaa gtcagggcca ccttgcactt gccaacgaat gctatggtgc ggccaagcca
     8881 agttttcaca gaaaaagaaa gtggcagtaa aagtaaatct aaaactcaaa acagtcgagt
     8941 ttttcagagt acaactattg atggtgaacg atcgccaata ctaggggcct ttaaaacggg
     9001 agcagctatt gcaaccattg acgactggta tcctgaagcc actgagccac taagggtcgg
     9061 acggtttggg gttcatcgcg aagatgtcac ttgctaccgt catccgtcta ccggaaaaga
     9121 ttttttctcg atattacaac aagcagagca ctatattgaa gtgttgagcg ccaacaaaac
     9181 tcccgctcaa gaaactatca acgacatgca ctttttaatg gctaacctga ttaagggtgg
     9241 gatgttccag cataaaggag actgactgtg aaatggtatt ataagacaat cacctttctg
     9301 ccagagttgt gcaacaacga gtcactggct gcaaagtgtc tccgcgttct gcatggattt
     9361 aactatcagt atgagacacg aaatatcggc gtttcatttc cgctttggtg tgatgcaacg
     9421 gttggaaaaa agatttcatt tgtcagcaag aacaagatag aactcgactt actacttaaa
     9481 caacactatt tcgtccaaat ggaacaactt caatactttc atatatccaa cactgttctc
     9541 gtcccagaag attgtacata cgtttccttt agacgctgtc aatctataga taagctcaca
     9601 gcagcagggc tggcaaggaa aatcagacgc ctggagaaac gtgctctatc tagaggcgag
     9661 caatttgacc catcatcttt tgctcaaaaa gagcatactg caatagcgca ctaccactca
     9721 cttggggagt ccagcaaaca gacgaaccgc aactttcgac tcaatatcag gatgctctcg
     9781 gagcaacccc gtgaggggaa ctcgattttt agtagctatg gcttatcaaa ttcagaaaac
     9841 tcgtttcagc ctgtaccctt aatctgacct ttaataagga gatataccat ggcgacaagt
     9901 ttacctacgc cctcagcaat tacgacttcg gcgttagagt atgcattcca tactcccgct
     9961 cgcaatctaa cgaaatctcg cggaaaaaac attcatcgtt atgtcagtgt aaagatgagt
    10021 aagaggatta cggtagaatc tactctagag tgtgatgcct gctatcactt tgattttgag
    10081 ccaagtattg ttcgcttttg cgctcaaccg attcgatttt tatattatct caatggtcag
    10141 tctcactcct atgttcctga ctttctagtt caatttgata ccaacgagtt tgttctatat
    10201 gaagtaaagt cagcttatgc taagaacaaa cctgattttg atgttgaatg ggaggcgaaa
    10261 gtaaaagcag caactgaact agggctagaa ttggagcttg ttgaagagag tgatattagg
    10321 gatacggttg tattaaataa tcttaagcgc atgcatcgtt atgcttcgaa agatgagctg
    10381 aataacgtac ataactctct cttaaaaata ataaagtaca atggcgccca atctgcaaga
    10441 tgcttgggag aacagttggg tttaaaaggc cgaactgttt taccaatttt gtgcgatttg
    10501 ctgtcaaggt gtttactcga tacacgtttg gataagcctc tatctcttga atctcgattt
    10561 gagttggcca gttatggcta agaaagggtt ctcaagtttc catagaaaag cagtctcttc
    10621 ccaagatacg cttgaatcga tagagcttgt ctctagcgct aattgtctag aaagtgttac
    10681 gtatcaagat atatcagcat ttcccgaaac aattgcggta gagattaatt tccgattaag
    10741 cattcttcgc tttttagcgc gaaagtgcga aaccattgtg gccaaatcaa ttgaaccaca
    10801 tcgtgtagag ctacagcaaa actatagtag aaaaataccc agtgcaataa cgatatatcg
    10861 atggtggctt gcttttcgaa aatcagacta caaccccatt agcttagcac ctaatatcaa
    10921 ggatagaggt aatagagaga caaaagtgtc aacagttgtt gattctatta tggaacaggc
    10981 agttgaaaga gttatatctg gacgaaaagt caatgttagc tctgcatata aacgtgttcg
    11041 acgaaaagtt cgtcaataca atctaactca tggaacgaaa tacacgtatc ctaagtacga
    11101 atctgtaaga aagcgagtaa aaaagaaaac cccatttgag ttattagccg cagggaaagg
    11161 ggagagagta gctaagagag agtttcgccg aatgggaaaa aagatcctca cgtctagcgt
    11221 gctagagagg gttgaaatag atcacactgt cgttgacctt tttgcagtac acgaagagta
    11281 tcgaatccca ttgggccgac cttggcttac tcaattggtt gattgttaca gtaaagctgt
    11341 aatcggtttt tatttaggtt tcgagcctcc tagctatgtg tcggtttccc ttgcacttaa
    11401 gaatgcaata caacgcaaag atgacttaat ctcctcgtat gaatcgatcg agaatgaatg
    11461 gctatgttat ggcatcccag acctactcgt aactgataat ggtaaagagt ttttgtcgaa
    11521 agcatttgat caagcatgtg aatcactatt gatcaatgtg catcaaaata aagttgagac
    11581 gcccgacaac aaacctcatg ttgaacgtaa ctacgggact attaatactt ctctgttaga
    11641 cgatttacct gggaaatcct tcagccagta ccttcaaaga gaagggtacg actctgtggg
    11701 agaagctacc cttacactca atgagattag agaaatttac ttaatttggt tggtggatat
    11761 ttatcataaa aaacccaatc agagaggcac taattgtcct aatgttgcgt ggaaaaaggg
    11821 ttgtcaagaa tgggaaccag aggagttctc tggttctaaa gacgaattag actttaaatt
    11881 tgctattgtt gattacaaac aacttactaa agtagggata actgtctaca aagaactgag
    11941 ttatagcaat gaccgtttag ctgaatatag agggaagaaa ggaaaccata aagttcagtt
    12001 caagtataac cctgagtgta tggcagttat ttgggtgttg gatgaggata tgaatgagta
    12061 ctttacagtt aatgcgattg actacgaata tgcaagtaga gtatcacttt ggcaacataa
    12121 atataacatg aaatatcaag cagaactaaa ttcagcagaa tatgatgagg acaaggaaat
    12181 tgatgcagaa ataaaaattg aagaaatcgc agatcgttca attgttaaga ctaacaaaat
    12241 cagagctcgg aggcgtggcg ctaggcatca agagaatagc gcaagggcta agtcaatcag
    12301 taatgcgaac ccggcctcga tacaaaaaca tgaagatgaa atcgttagtg cagataatga
    12361 cgattgggat attgattatg tctgagaaat cgtcaaatga gtgaaacgcg tgaggctcgt
    12421 atatcaagag ctaaaagggc atttgtatcc acaccgagcg ttaggaaaat cttgagttac
    12481 atggatagat gtagagatct atcagaccta gagtctgagc ctacatgcat gatggtctat
    12541 ggtgcttcgg gtgtaggtaa aacgaccgtc atcaagaaat acttaaatca aaacagaaga
    12601 gagtccgaag ccgggggcga tataataccg gttttgcata ttgagttgcc agacaatgcg
    12661 aagccagtag atgcagcaag ggaattgctg gttgaaatgg gtgacccgct agcactttat
    12721 gaaactgact tagctagatt gacgaaaaga ctgactgaat taatccctgc ggtcggcgtg
    12781 aagctgatta ttatcgatga gttccaacat ttggtggaag aaaggtcaaa tcgggttctt
    12841 acccaagtag gtaattggct aaaaatgata cttaacaaaa cgaaatgtcc aattgttata
    12901 tttggtatgc catactcaaa agttgtactg caagcaaact cgcaacttca cgggcgattt
    12961 tccattcagg ttgaactgcg cccctttagc taccagggag gtagaggtgt atttaaaact
    13021 tttttggaat accttgataa agccctacct tttgaaaaac aggctggc
//
