LOCUS       sequence-420900-         428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pCENTR_MGGAGM
FEATURES             Location/Qualifiers
     source          1..428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..75
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     67..84
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     68..84
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    100..199
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(221..320)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(345..363)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
       61 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
      121 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
      181 ttgtacaaaa aagcaggctt aatgggtggt gcaggaatga acccagcttt cttgtacaaa
      241 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      301 caaaataaaa tcattatttg ccatccagct gataagacga aagggcctcg tgatacgcct
      361 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      421 gggaaatg
//
