LOCUS       sequence-420904-        8534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 
            expression cells (BioID vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Roth JF, Braunschweig U, Wu M, Li JD, Lin ZY, Larsen B, Weatheritt 
            RJ, Gingras AC, Blencowe BJ
  TITLE     Systematic analysis of alternative exon-dependent interactome 
            remodeling reveals multitasking functions of gene regulatory 
            factors.
  JOURNAL   Mol Cell. 2023 Dec 7;83(23):4222-4238.e10. doi: 
            10.1016/j.molcel.2023.10.034.
  PUBMED    38065061
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.10"; journalName: "Mol Cell"; date: 
            "2023-12-7- 7"; volume: "83"; issue: "23"; pages: "4222-4238.e10"
COMMENT     Sequence Label: 5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5
FEATURES             Location/Qualifiers
     source          1..8534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..52
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2693..2717)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2730..2753)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(2784..3392,3393..3395,3396..3746))
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(3879..3903)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3925..3943
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3962..3980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3999..4017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4036..4054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4073..4091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4110..4128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4147..4165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4184..4202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4221..4239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4258..4276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4475..4494
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4569..4673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4674..4742,4743..5534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4892..4911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5705..6293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6194..6213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6447..6464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6617..6622,6623..6640,6641..6647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6660..6682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6679..6695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6679..6695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6744..6865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6755..6774)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6809..6828
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6995..8017)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(6995..8017)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(8025..8072)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8204..8223
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    join(8356..8534,1..46)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
       61 cgggtttaaa cgggccctct agactcgagt cacaccactt tgtacaagaa agttgggtat
      121 cacatacttg gggactgctg tgcagggaca cctggtgtgg cctgacgagg caaagtgtac
      181 atggctccca aaaactggtc ggcaatcctt cctgcttctc gaagcccact caacagagca
      241 ccatggactg tagctgggta gttccggatt gtgtgttctc cagcaaagaa gagtcttggg
      301 attggctgtg gggcacctgg aattgagggg ccaggagtga tcggctgagc cattaaatca
      361 tagtcatttc cagaggatcc tgcagccaca taagaatagg agccccgggc ccacggatca
      421 gcacgccaac gagataccac agtttccttg ggctgtggga ctgcactgct gccaaaaatc
      481 cctttgagaa tggccaggca ccggccgaca atcacatcat cactaatgtt ctccataatg
      541 ccagcagctt ctcctgctac cagggccaat agtattggag ctttatatag gttccagaag
      601 aggaagagct cacccctgct agcagttgta ctgccaacgt gcccaaacaa attgacactt
      661 gggtcccaga acacacggtc aaagcataac accaccttgt taaggttgcc aaatcccatc
      721 ctttggactg cagatgtttt ccactcagga agaggtggca caaactgaac agctggtggc
      781 tgctgcttca acactcccaa aggaagtgta cagagaactg catcacactt ataaataaag
      841 gtttgacttg tggaacgtgt gttcacagca atcacttcac atcctgaggc tgtgtagcga
      901 acctgccgca ctgctgtgtt cagtttaatg tccaagcctt cagctaaagc cacaggcaca
      961 catgagtagc cattccttac tgtcaggtgg cttccagtaa actcaaagtc atcatcctga
     1021 tcccaatgtt taagagagag ggtagagaga ggtgtggcgt tggcaaattc aagatttgca
     1081 aaatgccagt caagtatttg tctgtctctt gatgagaggt atacatcact tgggggattg
     1141 gcttccaatt cttgaagttt ttcttctagc tttccttgtg tttcagctaa ttcatcatat
     1201 tccttgcaga gggcagtcag gtccctgtgc ttgctcttca ccaggaactc ggctgtgata
     1261 tctctgggcg gcttcacttc tgaagcttct ttgtattgct gatggagctc tttaattttc
     1321 tccttcaaat ttaccatctt attaagaagc tctttcaact cctcctgagt tttcactatc
     1381 ttcttccaat gttcaatctg ctcatctttg acatgctttt cttgcagctg aatgacaacc
     1441 tccaatgcct ggccaaggga tacaggttta ttattgagga cgttgaagtc taactggtga
     1501 ctaaggtaag aagtggcttc tagcaaccgg ttaaattctt gttctaccat ttcatctttt
     1561 tcttttggaa ccttgacagt gtcagcttgt ccattggctt cataaagtgg gcatttttgc
     1621 ttgatcttgg ccagttccat atttacttgt ttgctgacga cagccatggg attccctcca
     1681 agacctgtta caaccatggc gccaagatca gctacatagt ttccttttcg aaatgtagca
     1741 actcgtccac ctactcgatc cctggcttcc agaagtgtga catccatccc aaaactctgt
     1801 agctgtcgag ctgctgccaa gccagaaaca cctgaaccta taataatcac ctttcctgtc
     1861 tttttaattg gtaagggttt tatcctcttg tagatgccga agttgataag accatggcgc
     1921 tctaagtaac tgtgaactcg gtggacaagc acagtatcgc tgttgtaagg cgcttccagc
     1981 tgctggagag tggcttcaaa cgtcagctgg atctttgggt tgtccagcca taactgcaat
     2041 gtgcgattcc tgatgaacag aaaaaccttc tgtgtctgct gaggcccact gatgatgtct
     2101 gggaaacagg ctgcttcctg agaggtcatt cggtcatggg gaagtcggct ttgaaaagct
     2161 gcaccctcca caccagacgg ctcttccggc tcactttcat tttcttcctc aggtggggct
     2221 tgaggaggtg gtgggggaag cttcttttcc ttctctgctt tagcatttct ttcttcttcc
     2281 gaataatatt catcttctga gaggttggcc aagctttcat ccatttctct gtattctacc
     2341 ttggcccgct tgcgccggct ggtccgtcgg ccctccgggg tctcggctat tccggtctcc
     2401 atgggcgtcg cggagccggg cccggctgtg ggccccgcct ggggcccagc agaccccggc
     2461 ggctcggcta ggcccccggg cggcgaggcc cgcggaggct ccttctttcg gggagtgcgc
     2521 tcgcccgccg cccccgtagc catgtcggtg gggcccgtca ggcccgcggg cggcgcggcc
     2581 acctcagagc cgttctcggc accgcccgcc gccccggacc cggcctcggt cccagcagcc
     2641 gccgccgccg ccgctgccgc tgccgccgcc gccgccttct tcccagacaa ggtgccaact
     2701 tttttgtaca aacttgtggg cgcgcctagt ttatcgtcat cgtctttgta gtctggcgcg
     2761 ccgccgccgg ggccagatcc ccccttctcg gcgctccgca ggctgatctc gccgcccatc
     2821 cagggcttga tgatgccgtc ctgctccagc agcagggcgc cctgcttgtc gatgccccgg
     2881 ctgatgccga agatctcctt gtcgccgatg atcagcttca cgggccggtt gatgaagttg
     2941 tccagcttct cccaccggct caggtagggg gccaggccct cctgctcgaa cagctccagg
     3001 gcggcccgca gctcccggat cagcatggcg gccagggtgt tccggtccag gttgatgccg
     3061 gcctcctgca gggtgatcca gccctggttc accacgctct cctccacccg ccgcatggcc
     3121 atgttgatgc cggcgccgat cacgatctgg gcggcgtcgc cggtcttgcc ggtcagctcc
     3181 accaggatgc cggccagctt ccggtcctgc aggtacaggt cgttgggcca cttcacccgc
     3241 accttgtcgg cgcccagctt ccgcagcacc tcggccatca cgatgccgat caccaggctc
     3301 aggccgatgg cggcggcggg gccctgctcc agccgccaga acatgctcag gtacaggttg
     3361 gcgccgaagg ggctgaacca cttccggccc cggccgcccc ggccggcctg ctggtactcg
     3421 gcgatgcagg cgtcgccgct cttcagctcg ccgatccggt ccagcaggta ctggttggtg
     3481 ctgtcgatca cgggcagcac ggccacgctg ccgccgtcca gctggcccag gatctgcttg
     3541 gcgttcagca gctggatggg ctcgggcagg ctgtagccct tgccgggcac ggtgaacacg
     3601 tccacgcccc agtcccgcag ggtctggatg tgcttgttga tggcggcccg gctcatgccc
     3661 agggtctcgc ccagctgctc gccgctgtgg aactcgccgt tggccagcag ggcgatcagc
     3721 ttcaggggca cggtgttgtc cttcatggtg gcgctagctg gatccgagct cggtaccaag
     3781 cttaagttta aacgctagag gatccagcct ccgcggaggc tggatcggtc ccggtgtctt
     3841 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     3901 gctctgctta tatagggggt accttctcta tcactgatag ggagctcgag acgtgtcgac
     3961 ttctctatca ctgataggga gacgttctag acgtaccttc tctatcactg atagggagct
     4021 cgagacgtgt cgacttctct atcactgata gggagacgtt ctagacgtac cttctctatc
     4081 actgataggg agctcgagac gtgtcgactt ctctatcact gatagggaga cgttctagac
     4141 gtaccttctc tatcactgat agggagctcg agacgtgtcg acttctctat cactgatagg
     4201 gagacgttct agacgtacct tctctatcac tgatagggag ctcgagacgt gtcgacttct
     4261 ctatcactga tagggagacg tgcggccagc ttggatccct cgagacgcgt atatctggcc
     4321 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
     4381 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
     4441 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4501 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg
     4561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6361 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6421 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6481 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6541 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6601 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6661 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
     6721 aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6781 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6841 cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc
     6901 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     6961 aggcggcggt ggaatcgaaa tctcgtagta cgtgctattc ctttgccctc ggacgagtgc
     7021 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7081 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7141 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7201 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7261 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7321 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7381 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7441 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7501 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7561 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7621 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7681 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     7741 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     7801 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     7861 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     7921 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     7981 acttctcgac agacgtcgcg gtgagttcag gctttttggc caaggaagtt cctatacttt
     8041 ctagagaata ggaacttcgg aataggaact tctaggtacg tgaaccatca ccctaatcaa
     8101 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8161 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8221 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8281 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     8341 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     8401 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     8461 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     8521 gggtcaagga aggc
//
