LOCUS       sequence-420906-        5304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for the expression of mouse ChREBP-alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Singh C, Jin B, Shrestha N, Markhard AL, Panda A, Calvo SE, Deik A, 
            Pan X, Zuckerman AL, Ben Saad A, Corey KE, Sjoquist J, Osganian S, 
            AminiTabrizi R, Rhee EP, Shah H, Goldberger O, Mullen AC, Cracan V, 
            Clish CB, Mootha VK, Goodman RP
  TITLE     ChREBP is activated by reductive stress and mediates GCKR-associated
            metabolic traits.
  JOURNAL   Cell Metab. 2024 Jan 2;36(1):144-158.e7. doi: 
            10.1016/j.cmet.2023.11.010. Epub 2023 Dec 14.
  PUBMED    38101397
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2023.11.010"; journalName: "Cell Metab"; date: 
            "2024-01-2- 2"; volume: "36"; issue: "1"; pages: "144-158.e7"
COMMENT     Sequence Label: pUC-ChREBP-alpha
FEATURES             Location/Qualifiers
     source          1..5304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3083..3099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3083..3099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3096..3118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3107..3123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3131..3137,3138..3155,3156..3161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3176..3197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3314..3331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3485..4073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3565..3584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4244..5035,5036..5104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4867..4886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5105..5209)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5277..5295
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgctag
      421 ccttaagaag cttatggcgc gcgcgctggc ggatctatcc gtgaacttgc aggtcccccg
      481 ggtcgtccct agcccggact cggactcgga tacggacttg gaggatccga gtccccggcg
      541 cagcgcgggt ggcctgcatc gatcacaggt catccacagc ggacacttca tggtgtcttc
      601 gccgcacagc gactcgctga cccggcgacg cgaccaggag gggcccgtgg ggctcgccga
      661 cttcgggccg cgcagcatcg atccgacact cacccacctc ttcgagtgct tgagcctggc
      721 ttacagtggc aagctggtct ctcccaagtg gaagaacttc aaaggcctca agttgctatg
      781 ccgggacaag atccggctga acaacgccat ctggagagcc tggtacattc agtatgtgca
      841 acggaggaag agcccagtgt gtggtttcgt gacccctctg caggggtctg aagcagatga
      901 gcaccggaaa cctgaggctg tcatcctgga gggtaattac tggaagcggc gcatcgaggt
      961 ggtgatgcgc gaataccaca agtggagaat ctactacaaa aagcggctcc gtaagtccag
     1021 cagggaaggg gatttcctgg ctcccaagca ggtggaaggt gggtggccgc caccagagcg
     1081 atggtgcgaa cagctcttct ccagcgtggt gcctgtgctg cttgggggct ccgaggagga
     1141 gcctgggggc cggcagcttc tagacctgga ctgcttcttg tccgatatct ccgacacact
     1201 cttcaccatg acacagccca gtccttcgtc cctgcagctg cccccagaag atgcttatgt
     1261 tggcaatgct gacatgatcc agccagacct gacgccgctg cagcccagcc tagatgactt
     1321 catggagata tcagatttct tcaccaatta ccgcccccct cagacaccca catcttcaaa
     1381 ctacatagag tcccccagct ttggccccat ggctgactcc ctcttcagca gtgggatcct
     1441 ggccccagag atgccatctc cagcctcgtc ttcctcctcc tcagggatga cccctcactc
     1501 agggaataca cgcctacagg ctcggaacag ctgctctgga cccttggatc ccaacccctt
     1561 tctgagctct gaattccttc ttcctgaaga ccctaagacc aagatcccac ctgctcctgg
     1621 accgacccct ctccttccat tccccacccc tgtcaaggtg catggcttag agccctgcac
     1681 cccctctccc ttccccacaa tggctccgcc cccctctttg ttgccagaag aatctctcct
     1741 ctctgccagg ttcccattca cctcagcccc tcctgctcca ggagtgtcta cgcttcctgc
     1801 acccacaacc tttgtcccca ccccacagcc cggccctggt cccgtcccct tctctgtaga
     1861 ccatctgccc catgggtacc tggaacccgt ctttgggcct cacttcactg tgcctcaggg
     1921 tatgcaaccc aggtgcaagc cctcctcccc atcccctggg ggacaaaaag caagtccacc
     1981 caccttggcc tctgccaccg ccagtcccac tgccactgcc actgccagag acaacaaccc
     2041 ctgtcttaca cagctgctca gggcagccaa gcctgagcaa gcactggagc ctccgactat
     2101 gcccggtacc ctcctccggc ctccagagtc cccgcaggat acagtttctg aaatcccccg
     2161 tgcccgtgcc tttttccccc caatcccggc ccctacacca ccccggccac ctccaggccc
     2221 agccacattg gctcctccca ggtccctggt tgtccccaaa gcagagcggc tctcgcctcc
     2281 agcctccagc ggcagtgagc ggcgactatc aggggatctc aactccatac aaccctcggg
     2341 ggcactgagt gtccacctgt ctccccctca aactgtccta agccggggtc gtgtagacaa
     2401 caacaagatg gagaaccgac gtatcacaca catctccgcg gagcagaaga ggcggttcaa
     2461 tattaagcta ggattcgaca ccctgcacgg acttgtcagc acgctcagtg cccagcccag
     2521 cctcaaggtg agcaaagcaa ccacgcttca gaagacagcg gagtacatcc tgatgctgca
     2581 gcaggaacgg gcagctatgc aggaggaggc gcagcagctg cgggatgaaa tagaggagct
     2641 caacgctgcc atcaacttgt gccagcagca gctaccggcc accggggtgc ccatcacaca
     2701 tcagcgcttt gaccagatgc gggacatgtt tgatgactat gtccggacgc gcacgctgca
     2761 caactggaag ttctgggtgt tcagcatcct catccgacct ttatttgaat ccttcaatgg
     2821 gatggtgtct accgccagct tgcacagcct ccgccagacc tcactggcct ggctggaaca
     2881 gtactgttcc ctacctgctc tccggccaac tgtcctgaat tcccttcgcc aactcagcac
     2941 ttccacaagc atcctgactg accccagcct tgtgcctgag caagccacac gggcagtcac
     3001 agagggcacc ctggggagac cattataatg tacagcggcc gcctcgagat cccaatggcg
     3061 cgccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3301 gctgttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4381 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5221 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5281 ggcgtatcac gaggcccttt tgtc
//
