LOCUS       sequence-420907-        3592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for the expression of mouse MLX-gamma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Singh C, Jin B, Shrestha N, Markhard AL, Panda A, Calvo SE, Deik A, 
            Pan X, Zuckerman AL, Ben Saad A, Corey KE, Sjoquist J, Osganian S, 
            AminiTabrizi R, Rhee EP, Shah H, Goldberger O, Mullen AC, Cracan V, 
            Clish CB, Mootha VK, Goodman RP
  TITLE     ChREBP is activated by reductive stress and mediates GCKR-associated
            metabolic traits.
  JOURNAL   Cell Metab. 2024 Jan 2;36(1):144-158.e7. doi: 
            10.1016/j.cmet.2023.11.010. Epub 2023 Dec 14.
  PUBMED    38101397
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2023.11.010"; journalName: "Cell Metab"; date: 
            "2024-01-2- 2"; volume: "36"; issue: "1"; pages: "144-158.e7"
COMMENT     Sequence Label: pUC-MLX-gamma
FEATURES             Location/Qualifiers
     source          1..3592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1371..1387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1371..1387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1384..1406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1395..1411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1419..1425,1426..1443,1444..1449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1464..1485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1602..1619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1773..2361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1853..1872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2532..3323,3324..3392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3155..3174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3393..3497)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3565..3583
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatgctag
      421 ccttaagatg acggagccgg gcgcctctcc ggaggaccct tgggtcaagg caagcttcgc
      481 ggacgcgcac gccggcgagg ggagggcggg tcgggctcgt gcacgtaggg ggtccggaag
      541 acgcggggct ccccaactat ccccagagtc tcccctgctg tccggggccc ggggctgcag
      601 agaagacagc tctcacccgg cctgtgccaa ggtcgagtat gcctacagtg acaacagcct
      661 ggatcctggg ctttttgtag aaagcaccca caaggggagt gtagtgtcca gagctaatag
      721 catcggctcc accagtgcct cttctgtccc caacacagat gatgaggaca gtgattatca
      781 gcaggagtcc tacaaggagt cctacaaaga ccggaggcgg cgtgcgcaca ctcaggctga
      841 acagaagaga agggatgcta ttaagagagg ctatgatgac cttcagacca ttgtccctac
      901 ctgccagcag caggatttct ccattggctc ccaaaaactc agcaaagcca tcgttctaca
      961 gaagaccatt gactacatcc agtttttaca caaggagaag aaaaagcagg aggaagaagt
     1021 atccacactg cgcaaggacg tcacagcctt gaagataatg aaagtgaact atgagcaaat
     1081 cgtgaaggca catcaggaca accctcatga gggagaggac caggtctctg accaggtcaa
     1141 gttcaacgtc tttcaaggca tcatggactc cctcttccag tctttcaacg cctctatctc
     1201 tgtggccagc ttccaggagc tctcagcttg tgtcttcagc tggattgagg aacactgtga
     1261 gccacagact ctacgagaga ttgtgatcgg tgtccttcat cagttgaaaa accaactcta
     1321 ctgatgtaca tctagaatcc caatggcgcg ccgagcttgg cgtaatcatg gtcatagctg
     1381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1561 gcggggagag gcggtttgcg tattgggcgc tgttccgctt cctcgctcac tgactcgctg
     1621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2641 ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta
     2701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3541 attatcatga cattaaccta taaaaatagg cgtatcacga ggcccttttg tc
//
