LOCUS       sequence-420911-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3- SREBP-1
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1097..1114)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1103..1327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1373..1801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1460..1479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1670..1691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1810..1830)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1815..2144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1995..2130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2057..2076
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2211..3005
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2265..2284)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2875..2894
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3179..3300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3216..3235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3270..3289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3349..3365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3349..3365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3362..3384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3373..3389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3397..3403,3404..3421,3422..3427))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3442..3463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3580..3597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3751..4339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3831..3850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4510..5301,5302..5370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5133..5152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5371..5475)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5550..5569)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5741..6120
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6121..6324
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6275..6295
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6369..6388
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6369..6387
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6413..6422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actgtgtgac ccagcccaac cccagccctg ggtcagctga tggggacaag gaattttcgg
       61 atgccctcgg gtacctgcag ctgctgaaca gctgttctga tgctgcgggg gctcctgcct
      121 acagcttctc catcagttcc agcatggcca ccaccaccgg cgtagacccg gtggccaagt
      181 ggtgggcctc tctgacagct gtggtgatcc actggctgcg gcgggatgag gaggcggctg
      241 agcggctgtg cccgctggtg gagcacctgc cccgggtgct gcaggagtct gagagacccc
      301 tgcccagggc agctctgcac tccttcaagg ctgcccgggc cctgctgggc tgtgccaagg
      361 cagagtctgg tccagccagc ctgaccatct gtgagaaggc cagtgggtac ctgcaggaca
      421 gcctggctac cacaccagcc agcagctcca ttgacaaggc cgtgcagctg ttcctgtgtg
      481 acctgcttct tgtggtgcgc accagcctgt ggcggcagca gcagcccccg gccccggccc
      541 cagcagccca gggcaccagc agcaggcccc aggcttccgc ccttgagctg cgtggcttcc
      601 aacgggacct gagcagcctg aggcggctgg cacagagctt ccggcccgcc atgcggaggg
      661 tgttcctaca tgaggccacg gcccggctga tggcgggggc cagccccaca cggacacacc
      721 agctcctcga ccgcagtctg aggcggcggg caggccccgg tggcaaagga ggcgcggtgg
      781 cggagctgga gccgcggccc acgcggcggg agcacgcgga ggccttgctg ctggcctcct
      841 gctacctgcc ccccggcttc ctgtcggcgc ccgggcagcg cgtgggcatg ctggctgagg
      901 cggcgcgcac actagagaaa cttggcgatc gccggctgct gcacgactgt cagcagatgc
      961 tcatgcgcct gggcggtggg accactgtca cttccagcgg agaattctca ggcgcccccg
     1021 tgccctaccc cgaccccctg gagccccggt gagcggccgc tcgagtctag tgggcccgtt
     1081 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     1141 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1201 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1261 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     1321 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     1381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1441 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1501 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1561 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1681 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1741 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1801 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     1861 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     1921 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     1981 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2041 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     2101 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     2161 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     2221 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2281 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     2341 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     2401 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     2461 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     2521 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     2581 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     2641 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     2701 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     2761 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2821 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2881 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2941 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3001 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3061 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3121 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     3181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3301 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     3361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     5521 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5581 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     5641 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     5701 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     5761 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5821 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5881 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5941 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6001 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6061 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6121 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     6181 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6241 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     6301 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     6361 atcgaaatta atacgactca ctatagggag acccaagctg gctagcggat ccgccaccat
     6421 ggcctctatg accggcggcc agcaaatggg tatggcctct atgaccggcg gccagcaaat
     6481 gggagcatcg atggacgagc cacccttcag cgaggcggct ttggagcagg cgctgggcga
     6541 gccgtgcgat ctggacgcgg cgctgctgac cgacatcgaa ggtgaagtcg gcgcggggag
     6601 gggtagggcc aacggcctgg acgccccaag ggcgggcgca gatcgcggag ctatggattg
     6661 cactttcgaa gacatgcttc agcttatcaa caaccaagac agtgacttcc ctggcctatt
     6721 tgacccaccc tatgctggga gtggggcagg gggcacagac cctgccagcc ccgataccag
     6781 ctccccaggc agcttgtctc cacctcctgc cacattgagc tcctctcttg aagccttcct
     6841 gagcgggccg caggcagcgc cctcacccct gtcccctccc cagcctgcac ccactccatt
     6901 gaagatgtac ccgtccatgc ccgctttctc ccctgggcct ggtatcaagg aagagtcagt
     6961 gccactgagc atcctgcaga cccccacccc acagcccctg ccaggggccc tcctgccaca
     7021 gagcttccca gccccagccc caccgcagtt cagctccacc cctgtgttag gctaccccag
     7081 ccctccggga ggcttctcta caggaagccc tcccgggaac acccagcagc cgctgcctgg
     7141 cctgccactg gcttccccgc caggggtccc gcccgtctcc ttgcacaccc aggtccagag
     7201 tgtggtcccc cagcagctac tgacagtcac agctgccccc acggcagccc ctgtaacgac
     7261 cactgtgacc tcgcagatcc agcaggtccc ggtcctgctg cagccccact tcatcaaggc
     7321 agactcgctg cttctgacag ccatgaagac agacggagcc actgtgaagg cggcaggtct
     7381 cagtcccctg gtctctggca ccactgtgca gacagggcct ttgccgaccc tggtgagtgg
     7441 cggaaccatc ttggcaacag tcccactggt cgtagatgcg gagaagctgc ctatcaaccg
     7501 gctcgcagct ggcagcaagg ccccggcctc tgcccagagc cgtggagaga agcgcacagc
     7561 ccacaacgcc attgagaagc gctaccgctc ctccatcaat gacaaaatca ttgagctcaa
     7621 ggatctggtg gtgggcactg aggcaaagct gaataaatct gctgtcttgc gcaaggccat
     7681 cgactacatt cgctttctgc aacacagcaa ccagaagctt aagcaggaga acctaagtct
     7741 gcgcactgct gtccacaaaa gcaaatctct gaaggatctg gtgtcggcct gtggcagtgg
     7801 agggaacaca gacgtgctca tggagggcgt gaagactgag gtggaggaca cactgacccc
     7861 acccccctcg gatgctggct cacctttcca gagcagcccc ttgtcccttg gcagcagggg
     7921 cagtggcagc ggtggcagtg gcagtgactc ggagcctgac agcccagtct ttgaggacag
     7981 caaggcaaag ccagagcagc ggccgtctct gcacagccgc ggcatgctgg accgctcccg
     8041 cctggccctg tgcacgctcg tcttcctctg cctgtcctgc aaccccttgg cctccttgct
     8101 gggggcccgg gggcttccca gcccctcaga taccaccagc gtctaccata gccctgggcg
     8161 caacgtgctg ggcaccgagt ctagagatgg ccctggctgg gcccagtggc tgctgccccc
     8221 agtggtctgg ctgctcaatg ggctgttggt gctcgtctcc ttggtgcttc tctttgtcta
     8281 cggtgagcca gtcacacggc cccactcagg ccccgccgtg tacttctgga ggcatcgcaa
     8341 gcaggctgac ctggacctgg cccggggaga ctttgcccag gctgcccagc agctgtggct
     8401 ggccctgcgg gcactgggcc ggcccctgcc cacctcccac ctggacctgg cttgtagcct
     8461 cctctggaac ctcatccgtc acctgctgca gcgtctctgg gtgggccgct ggctggcagg
     8521 ccgggcaggg ggcctgcagc aggactgtgc tctgcgagtg gatgcaagcg ccagcgcccg
     8581 agacgcagcc ctggtctacc ataagctgca ccagctgcac accatgggga agcacacagg
     8641 cgggcacctc actgccacca acctggcgct gagtgccctg aacctggcag agtgtgcagg
     8701 ggatgccgtg tctgtggcga cgctggccga gatctatgtg gcggctgcat tgagagtgaa
     8761 gaccagtctc ccacgggcct tgcattttct gacacgcttc ttcctgagca gtgcccgcca
     8821 ggcctgcctg gcacagagtg gctcagtgcc tcctgccatg cagtggctct gccaccccgt
     8881 gggccaccgt ttcttcgtgg atggggactg gtccgtgctc agtaccccat gggagagcct
     8941 gtacagcttg gccgggaacc cagtggaccc cctggcccag gtgactcagc tattccggga
     9001 acatctctta gagcgagcac tga
//
