LOCUS       sequence-420920-        4728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9A7_STOP
FEATURES             Location/Qualifiers
     source          1..4728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2853..2952)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2969..2988)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2970..2988)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2993..3009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2993..3009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3111..3130)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3122..3931
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3189..3208)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4078..4666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4567..4586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagc ctggcgatgc cgccaggcca ggaagcggaa gggcaaccgg
      721 agcaccccct ccaaggctgc tgctgctgcc actgctgctg ggatggggac tgagagtggc
      781 agcagcagcc tccgccagct cctctggcgc cgccgccgag gatagctccg ccatggagga
      841 gctggccacc gagaaggagg ccgaggagtc tcacaggcag gactctgtga gcctgctgac
      901 attcatcctg ctgctgaccc tgacaatcct gaccatctgg ctgtttaagc accggagagt
      961 gcgcttcctg cacgagaccg gactggcaat gatctacggc ctgatcgtgg gcgtgatcct
     1021 gagatatgga accccagcca catccggaag ggataagtcc ctgtcttgca cccaggagga
     1081 cagggccttt tccacactgc tggtgaacgt gtctggcaag ttctttgagt acacactgaa
     1141 gggcgagatc tctcccggca agatcaacag cgtggagcag aatgatatgc tgcgcaaggt
     1201 gaccttcgac cctgaggtgt tctttaatat cctgctgccc cctatcatct ttcacgccgg
     1261 ctactccctg aagaagcggc acttctttag aaacctgggc tctatcctgg cctatgcctt
     1321 tctgggcaca gccgtgagct gcttcatcat cggcaatctg atgtacggcg tggtgaagct
     1381 gatgaagatc atgggccagc tgtccgataa gttctactat accgactgtc tgttctttgg
     1441 cgccatcatc tctgccaccg accccgtgac agtgctggcc atcttcaacg agctgcacgc
     1501 cgacgtggat ctgtatgccc tgctgtttgg cgagagcgtg ctgaatgatg ccgtggccat
     1561 cgtgctgtct agctccatcg tggcatacca gcctgcaggc ctgaacaccc acgccttcga
     1621 cgcagccgcc ttctttaagt ccgtgggcat cttcctgggc atctttagcg gctccttcac
     1681 catgggcgcc gtgacaggcg tgaacgccaa tgtgaccaag tttacaaagc tgcactgttt
     1741 ccctctgctg gagaccgccc tgttctttct gatgtcttgg agcacatttc tgctggcaga
     1801 ggcatgcggc ttcaccggag tggtggccgt gctgttttgt ggcatcaccc aggcccacta
     1861 cacatataac aatctgagcg tggagtccag gtctcgcaca aagcagctgt ttgaggtgct
     1921 gcacttcctg gccgagaatt tcatctttag ctatatgggc ctggccctgt tcacctttca
     1981 gaagcacgtg ttttccccta tcttcatcat cggcgccttt gtggcaatct tcctgggaag
     2041 ggcagcacac atctacccac tgtccttctt tctgaacctg ggcaggcgcc acaagatcgg
     2101 ctggaatttt cagcacatga tgatgttcag cggactgaga ggagcaatgg ccttcgccct
     2161 ggcaatcagg gatacagcct cctatgccag acagatgatg tttaccacaa ccctgctgat
     2221 cgtgttcttt accgtgtgga tcatcggcgg cggcacaacc ccaatgctgt cctggctgaa
     2281 catcagggtg ggagtggagg agccatctga ggaggatcag aatgagcacc actggcagta
     2341 cttcagagtg ggagtggacc cagatcagga cccaccacct aacaatgact cttttcaggt
     2401 gctgcagggc gatggaccag acagcgccag gggaaacaga acaaagcagg agagcgcctg
     2461 gatctttcgg ctgtggtact ccttcgatca caattatctg aagcctatcc tgacccacag
     2521 cggcccaccc ctgacaacca cactgccagc atggtgcgga ctgctggcaa ggtgtctgac
     2581 atctccccag gtgtatgaca accaggagcc tctgcgcgag gaggatagcg acttcatcct
     2641 gaccgagggc gatctgaccc tgacatacgg cgactctacc gtgacagcca atggctctag
     2701 ctcctctcac accgcaagca catccctgga gggaagccgg agaacaaaga gctcctctga
     2761 ggaggtgctg gagcgggatc tgggcatggg cgaccagaag gtgagctcca ggggaaccag
     2821 gctggtgttc ccactggagg acaacgccta agacccagct ttcttgtaca aagttggcat
     2881 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2941 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     3001 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3061 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     3121 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     3181 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3241 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3301 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     3361 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3421 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3481 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3541 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3601 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3661 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3721 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3781 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3841 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3901 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     3961 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     4021 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4201 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
