LOCUS       sequence-420927-        4113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A14_STOP
FEATURES             Location/Qualifiers
     source          1..4113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2238..2337)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2354..2373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2355..2373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2378..2394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2378..2394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2496..2515)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2507..3316
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2574..2593)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3463..4051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3952..3971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagt ttcacaatgg aggacacgtg tctggaatcg gaggcttcct
      721 ggtgagcctg acctccagga tgaagcctca cacactggcc gtgaccccag ccctgatctt
      781 cgccatcaca gtggccacca tcggctcctt ccagtttggc tacaacaccg gcgtgatcaa
      841 tgccccagag acaatcatca aggagtttat caataagaca ctgaccgaca aggcaaacgc
      901 accaccttcc gaggtgctgc tgacaaacct gtggtctctg agcgtggcca tcttttctgt
      961 gggcggcatg atcggctcct tctctgtggg cctgtttgtg aaccgcttcg gccggagaaa
     1021 ttctatgctg atcgtgaacc tgctggcagc aaccggagga tgcctgatgg gcctgtgcaa
     1081 gatcgccgag agcgtggaga tgctgatcct gggccggctg gtcatcggcc tgttttgcgg
     1141 cctgtgcaca ggcttcgtgc ccatgtatat cggagagatc agccctaccg ccctgagggg
     1201 agcctttggc acactgaatc agctgggcat cgtgatcggc atcctggtgg cccagatctt
     1261 cggcctggag ctgatcctgg gctccgagga gctgtggcca gtgctgctgg gctttaccat
     1321 cctgccagca atcctgcagt ccgccgccct gccttgctgt ccagagtctc ccaggttcct
     1381 gctgatcaat cgcaagaagg aggagaacgc aaccaggatc ctgcagcgcc tgtggggcac
     1441 acaggacgtg agccaggata tccaggagat gaaggacgag tctgcccgga tgagccagga
     1501 gaagcaggtg accgtgctgg agctgttccg ggtgagctcc tacagacagc ccatcatcat
     1561 cagcatcgtg ctgcagctgt cccagcagct gtctggcatc aatgccgtgt tttactattc
     1621 caccggcatc ttcaaggatg ccggcgtgca gcagcctatc tatgccacaa tctctgccgg
     1681 cgtggtgaac acaatcttta ccctgctgag cctgttcctg gtggagaggg caggcaggcg
     1741 caccctgcac atgatcggac tgggaggcat ggccttctgc tctacactga tgaccgtgag
     1801 cctgctgctg aagaaccact acaatggcat gagcttcgtg tgcatcggcg ccatcctggt
     1861 gtttgtggcc tgtttcgaga tcggcccagg ccccatccct tggtttatcg tggccgagct
     1921 gttctcccag ggaccaagac cagcagcaat ggcagtggcc ggctgtagca actggacctc
     1981 caattttctg gtgggcctgc tgttcccttc tgccgcctac tatctgggcg cctacgtgtt
     2041 catcatcttt acaggcttcc tgatcacctt tctggccttc acattcttta aggtgccaga
     2101 gacacggggc agaacctttg aggatatcac aagggccttc gagggacagg cacacggagc
     2161 agacagaagc ggcaaggatg gcgtgatggg catgaattcc atcgagcctg ccaaggagac
     2221 cacaaccaac gtgtaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2281 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2341 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2401 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2461 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2521 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2581 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2641 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2701 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2761 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2821 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2881 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2941 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3001 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3061 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3121 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3181 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3241 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3301 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3361 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4081 cctggccttt tgctggcctt ttgctcacat gtt
//
