LOCUS       sequence-420948-        6948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes bacterial crtEBIY operon under the control of synthetic 
            promoter responsive to JUB1-derived artificial transcription factor 
            in bacterial cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Naseri G, Raasch H, Charpentier E, Erhardt M
  TITLE     A versatile regulatory toolkit of arabinose-inducible artificial 
            transcription factors for Enterobacteriaceae.
  JOURNAL   Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3.
  PUBMED    37789111
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-3"; volume: "6(1):1005. doi"; pages: " 
            10.1038/s42003-023-05363-3"
COMMENT     Sequence Label: pCAROTENE5X
FEATURES             Location/Qualifiers
     source          1..6948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        310..344
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     RBS             354..365
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4944..5030
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5067..5084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5280..5299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5783..5877)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5907..6698,6699..6767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6530..6549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6768..6872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(6940..6948,1..10)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcacgaggc cctttcgtct tcacctcgag gatcccgggt acctgcagct agcgtcgact
       61 gtggtctcta caggaattct ttcttctgtc gccgtcttgt caacggcacc agttatatat
      121 ttcttctgtc gccgtcttgt caacggcacc agttatatat tggtttcttc tgtcgccgtc
      181 ttgtcaacgg caccagttat atatttcttc tgtcgccgtc ttgtcaacgg caccagttat
      241 atatttcttc tgtcgccgtc ttgtcaacgg caccagttat atattgggtt ccaccggata
      301 cctccggact tgacagctag ctcagtccta gggattgtgc tagcgaattc attaaagagg
      361 agaaaggtac catgacggtc tgcgcaaaaa aacacgttca tctcactcgc gatgctgcgg
      421 agcagttact ggctgatatt gatcgacgcc ttgatcagtt attgcccgtg gagggagaac
      481 gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc accgggaaaa cgtattcgcc
      541 ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc tgtcagccat gacggattac
      601 tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc gctgatcctt gacgatatgc
      661 cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac cattcattct cattacggag
      721 agcatgtggc aatactggcg gcggttgcct tgctgagtaa agcctttggc gtaattgccg
      781 atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt ttctgaactg tcaaacgcca
      841 tcggcatgca aggattggtt cagggtcagt tcaaggatct gtctgaaggg gataagccgc
      901 gcagcgctga agctattttg atgacgaatc actttaaaac cagcacgctg ttttgtgcct
      961 ccatgcagat ggcctcgatt gttgcgaatg cctccagcga agcgcgtgat tgcctgcatc
     1021 gtttttcact tgatcttggt caggcatttc aactgctgga cgatttgacc gatggcatga
     1081 ccgacaccgg taaggatagc aatcaggacg ccggtaaatc gacgctggtc aatctgttag
     1141 gcccgagggc ggttgaagaa cgtctgagac aacatcttca gcttgccagt gagcatctct
     1201 ctgcggcctg ccaacacggg cacgccactc aacattttat tcaggcctgg tttgacaaaa
     1261 aactcgctgc cgtcagttaa taaactagag ctcaaggagg tactagatga ataatccgtc
     1321 gttactcaat catgcggtcg aaacgatggc agttggctcg aaaagttttg cgacagcctc
     1381 aaagttattt gatgcaaaaa cccggcgcag cgtactgatg ctctacgcct ggtgccgcca
     1441 ttgtgacgat gttattgacg atcagacgct gggctttcag gcccggcagc ctgccttaca
     1501 aacgcccgaa caacgtctga tgcaacttga gatgaaaacg cgccaggcct atgcaggatc
     1561 gcagatgcac gaaccggcgt ttgcggcttt tcaggaagtg gctatggctc atgatatcgc
     1621 cccggcttac gcgtttgatc atctggaagg cttcgccatg gatgtacgcg aagcgcaata
     1681 cagccaactg gatgatacgc tgcgctattg ctatcacgtt gcaggcgttg tcggcttgat
     1741 gatggcgcaa atcatgggcg tgcgggataa cgccacgctg gaccgcgcct gtgaccttgg
     1801 gctggcattt cagttgacca atattgctcg cgatattgtg gacgatgcgc atgcgggcca
     1861 ctgttatctg ccggcaagct ggctggagca tgaaggtctg aacaaagaga attatgcggc
     1921 acctgaaaac cgtcaggcgc tgagccgtat cgcccgtcgt ttggtgcagg aagcagaacc
     1981 ttactatttg tctgccacag ccggcctggc agggttgccc ctgcgttccg cctgggcaat
     2041 cgctacggcg aagcaggttt accggaaaat aggtgtcaaa gttgaacagg ccggtcagca
     2101 agcctgggat cagcggcagt caacgaccac gcccgaaaaa ttaacgctgc tgctggccgc
     2161 ctctggtcag gcccttactt cccggatgcg ggctcatcct ccccgccctg cgcatctctg
     2221 gcagcgcccg ctctaataat actagagctc aaggaggtac tagatgaaac caactacggt
     2281 aattggtgca ggcttcggtg gcctggcact ggcaattcgt ctacaagctg cggggatccc
     2341 cgtcttactg cttgaacaac gtgataaacc cggcggtcgg gcttatgtct acgaggatca
     2401 ggggtttacc tttgatgcag gcccgacggt tatcaccgat cccagtgcca ttgaagaact
     2461 gtttgcactg gcaggaaaac agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt
     2521 ttaccgcctg tgttgggagt cagggaaggt ctttaattac gataacgatc aaacccggct
     2581 cgaagcgcag attcagcagt ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga
     2641 ctattcacgc gcggtgttta aagaaggcta tctaaagctc ggtactgtcc cttttttatc
     2701 gttcagagac atgcttcgcg ccgcacctca actggcgaaa ctgcaagcat ggagaagcgt
     2761 ttacagtaag gttgccagtt acatcgaaga tgaacatctg cgccaggcgt tttctttcca
     2821 ctcgctgttg gtgggcggca atcccttcgc cacctcatcc atttatacgt tgatacacgc
     2881 gctggagcgt gagtggggcg tctggtttcc gcgtggcggc accggcgcat tagttcaggg
     2941 gatgataaag ctgtttcagg atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca
     3001 tatggaaacg acaggaaaca agattgaagc cgtgcattta gaggacggtc gcaggttcct
     3061 gacgcaagcc gtcgcgtcaa atgcagatgt ggttcatacc tatcgcgacc tgttaagcca
     3121 gcaccctgcc gcggttaagc agtccaacaa actgcaaact aagcgcatga gtaactctct
     3181 gtttgtgctc tattttggtt tgaatcacca tcatgatcag ctcgcgcatc acacggtttg
     3241 tttcggcccg cgttaccgcg agctgattga cgaaattttt aatcatgatg gcctcgcaga
     3301 ggacttctca ctttatctgc acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg
     3361 ttgcggcagt tactatgtgt tggcgccggt gccgcattta ggcaccgcga acctcgactg
     3421 gacggttgag gggccaaaac tacgcgaccg tatttttgcg taccttgagc agcattacat
     3481 gcctggctta cggagtcagc tggtcacgca ccggatgttt acgccgtttg attttcgcga
     3541 ccagcttaat gcctatcatg gctcagcctt ttctgtggag cccgttctta cccagagcgc
     3601 ctggtttcgg ccgcataacc gcgataaaac cattactaat ctctacctgg tcggcgcagg
     3661 cacgcatccc ggcgcaggca ttcctggcgt catcggctcg gcaaaagcga cagcaggttt
     3721 gatgctggag gatctgatat aataatacta gaggaggtac tagatgcaac cgcattatga
     3781 tctgattctc gtgggggctg gactcgcgaa tggccttatc gccctgcgtc ttcagcagca
     3841 gcaacctgat atgcgtattt tgcttatcga cgccgcaccc caggcgggcg ggaatcatac
     3901 gtggtcattt caccacgatg atttgactga gagccaacat cgttggatag ctccgctggt
     3961 ggttcatcac tggcccgact atcaggtacg ctttcccaca cgccgtcgta agctgaacag
     4021 cggctacttt tgtattactt ctcagcgttt cgctgaggtt ttacagcgac agtttggccc
     4081 gcacttgtgg atggataccg cggtcgcaga ggttaatgcg gaatctgttc ggttgaaaaa
     4141 gggtcaggtt atcggtgccc gcgcggtgat tgacgggcgg ggttatgcgg caaattcagc
     4201 actgagcgtg ggcttccagg cgtttattgg ccaggaatgg cgattgagcc acccgcatgg
     4261 tttatcgtct cccattatca tggatgccac ggtcgatcag caaaatggtt atcgcttcgt
     4321 gtacagcctg ccgctctcgc cgaccagatt gttaattgaa gacacgcact atattgataa
     4381 tgcgacatta gatcctgaat gcgcgcggca aaatatttgc gactatgccg cgcaacaggg
     4441 ttggcagctt cagacactgc tgcgagaaga acagggcgcc ttacccatta ctctgtcggg
     4501 caatgccgac gcattctggc agcagcgccc cctggcctgt agtggattac gtgccggtct
     4561 gttccatcct accaccggct attcactgcc gctggcggtt gccgtggccg accgcctgag
     4621 tgcacttgat gtctttacgt cggcctcaat tcaccatgcc attacgcatt ttgcccgcga
     4681 gcgctggcag cagcagggct ttttccgcat gctgaatcgc atgctgtttt tagccggacc
     4741 cgccgattca cgctggcggg ttatgcagcg tttttatggt ttacctgaag atttaattgc
     4801 ccgtttttat gcgggaaaac tcacgctgac cgatcggcta cgtattctga gcggcaagcc
     4861 gcctgttccg gtattagcag cattgcaagc cattatgacg actcatcgtt aataaactag
     4921 ttaagcggcc gctctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4981 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     5041 agacctaggc ggagatattc cgcttcctcg ctcactgact cgctccgctc ggtcgttcgg
     5101 ctgcggcgag cggtaccggc ttactggcgg ggcggaaatt tcctggaaga tgccaggaag
     5161 aaacttaaca gggaagcgat aaggccacgg cagagccgtt tttccatagg ctccgccccc
     5221 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactta
     5281 aagataccag gcgtttcccc ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg
     5341 gtttaccggt gtcactccgc tgttatggcc gcgtttatct cattccacgc ctgacactcg
     5401 gttccgggaa ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     5461 actgctgcgc ctgttccggt aactatcgac ttgagtccaa gccggaaaga cacgacaaag
     5521 cgccactggc agcagccact ggtaagaggg tgtacaagag attactgatg cagagttctt
     5581 gaagtcagag ccaaactacg gctaaactgg aaggacgaat ttggtggcag cggtcttgta
     5641 caccctgtta ccacggttca gaagttcccc aacttactga accttcgaaa aaccacctcc
     5701 ccaggtggtt ttttcgtttt cagagcaaga gattacgacg caacggaaag gatctcaaga
     5761 ggttcatctt actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     5821 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     5881 gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5941 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6001 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6061 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6121 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6181 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6241 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6301 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6361 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6421 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     6901 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgt
//
