LOCUS       sequence-420994-        8971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DNER-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..8971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     115..134
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     140..159
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      245..833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     734..753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     987..1004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1102..1119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1103..1119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1575..1591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1758..2137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2138..2341
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2292..2312
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2338..2362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2572..2590
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2572..2589
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2603..2622
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      4410..4419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4567..4602
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4612..4635
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6295..6339
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6340..6366
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6506..6523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6512..6623
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6741..6761)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6746..7075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6926..7061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6988..7007
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7123..7170
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7189..7788
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7189..7208)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7685..7705
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7918..8039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7955..7974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8009..8028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8092..8883,8884..8952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8715..8734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8953..8971,1..73))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
       61 aaaaataaac aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg
      121 agcccattta tacctgaata tggctcataa caccccttgc tcatgaccaa aatcccttaa
      181 cgtgagttac gcgcgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttagcccacc
      421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      901 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      961 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaggcgaga
     1021 gtagggaact gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt
     1081 ttctacaaac tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg
     1141 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
     1201 ttttttatgg ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca
     1261 ctttgcttga tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta
     1321 aataagatac gttgcttttt cgattgatga acacctataa ttaaactatt catctattat
     1381 ttatgatttt ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc
     1441 tcgaaaataa taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat
     1501 acaaaatata atacaaacta taagatgtta tcagtattta ttatcattta gaataaattt
     1561 tgtgtcgccc ttaattgtga gcggataaca attacgagct tcatgcacag tggcgttgac
     1621 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
     1681 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     1741 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
     1801 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1861 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1921 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1981 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2041 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2101 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2161 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2221 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2281 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2341 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2401 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     2461 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
     2521 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     2581 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     2641 ctgcacctcg gttctatcga aaacgcgcct gctagcgcca ccatgaagac catcattgcc
     2701 ctgagctaca tcttctgcct ggtgttcgct gcggccgcca atcctgttcc tgctgctcct
     2761 ctttctgccc ctggaccttg tgctgcccag ccttgtagaa atggcggcgt gtgtaccagc
     2821 agacccgagc ctgatcctca gcatcctgct ccagctggcg agcctggcta ctcttgtaca
     2881 tgtcctgccg gaatcagcgg cgccaattgt cagctggtgg ccgatccttg tgccagcaat
     2941 ccttgccacc acggcaattg cagcagcagc tctagcagct cctccgacgg ctacctgtgc
     3001 atctgcaatg agggctacga gggccccaat tgcgaacagg ctctgccttc tctgcctgcc
     3061 actggctgga cagaatctat ggcccctaga cagctgcagc ccgtgccagc tacacaagag
     3121 cccgataaga tcctgcctcg gagccaggct acagtgaccc tgcctacatg gcagcctaag
     3181 accggccaga aagtggtgga aatgaagtgg gaccaagtgg aagtgatccc cgatatcgcc
     3241 tgcggcaacg ccagcagcaa ttcttctgct ggcggcagac tggtgtcctt cgaggtgcca
     3301 cagaacacca gcgtgaagat cagacaggat gccaccgcca gcctgattct gctgtggaaa
     3361 gtgaccgcca ccggcttcca gcagtgctct ctgatcgatg gcagaagcgt gacacccctg
     3421 caagcaagcg gaggactggt gctgctcgaa gaaatgctgg ccctgggcaa caaccacttc
     3481 atcggcttcg tgaacgacag cgtgaccaag agcatcgtgg ccctgagact gaccctggtg
     3541 gtcaaggtgt ccacatgtgt gcctggcgag agccacgcca acgatctgga atgttctggc
     3601 aagggcaagt gcaccaccaa gcctagcgaa gccaccttca gctgtacctg cgaggaacag
     3661 tacgtgggca ccttctgcga agagtacgac gcctgccaga gaaagccctg tcagaacaac
     3721 gcctcctgca tcgacgccaa tgagaagcag gacggcagca acttcacctg tgtgtgtctg
     3781 cctggctata ccggcgagct gtgccagagc aagatcgact actgcattct ggacccctgc
     3841 agaaacggcg ccacctgtat ctctagcctg agcggcttta cctgtcagtg ccccgagggc
     3901 tattttggca gcgcctgtga agagaaggtg gacccttgtg caagctcccc atgccaaaac
     3961 aacggcacct gttacgtgga cggcgtgcac tttacctgca actgcagccc aggcttcact
     4021 ggccctacat gtgcccagct gatcgacttc tgtgctctga gcccttgcgc tcacggcaca
     4081 tgcagatctg tgggcaccag ctacaagtgc ctgtgcgatc ctggctatca cggcctgtat
     4141 tgcgaagagg agtacaacga gtgcctgagc gccccttgtc tgaatgccgc cacttgcaga
     4201 gatctggtca acggctacga gtgcgtgtgt ctggccgagt acaagggcac ccactgcgag
     4261 ctgtacaagg acccatgtgc caacgtgtcc tgcctgaacg gcgctacatg tgatagcgac
     4321 ggcctgaatg gaacctgcat ctgcgcccct gggtttaccg gcgaagagtg cgatatcgac
     4381 atcaatgagt gcgacagcaa cccctgtcac catggcggct cttgtctgga ccagcctaac
     4441 ggctacaact gccactgtcc tcacggatgg gtcggagcca actgcgagat tcatctgcag
     4501 tggaagtccg gccacatggc cgagagcctg accaacatgc ctcggcacag tctgtatggc
     4561 gcgccggaag atcaagttga tccaagattg attgatggca agggcggatc tctggaggtg
     4621 ctgttccagg gaccaggcgg aagcggcaat cccggcggcg atctggcccc ccagatgctg
     4681 cgcgagctgc aggagaccaa tgccgccctg caggacgtgc gcgagctgct gcgccaccgc
     4741 gtgaaggaga tcaccttcct gaagaacacc gtgatggagt gcgacgcctg cggtatgcag
     4801 ccctctagag gctccggctc catcatccca gttgaggagg agaacccgga cttctggaac
     4861 cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc
     4921 aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg
     4981 atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc
     5041 ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga
     5101 gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt
     5161 gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg
     5221 aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac
     5281 gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac
     5341 gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac
     5401 atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca
     5461 gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg
     5521 gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc
     5581 atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac
     5641 atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag
     5701 gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt
     5761 cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg
     5821 ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc
     5881 gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc
     5941 atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac
     6001 ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag
     6061 agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca
     6121 ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag
     6181 gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc
     6241 tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtgg cggaggactg
     6301 aacgacatct tcgaggctca gaaaatcgaa tggcacgaac atcaccatca ccatcaccat
     6361 caccattaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     6421 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     6481 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6541 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6601 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     6661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6721 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6901 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7081 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7141 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
     7201 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     7261 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     7321 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     7381 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     7441 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     7501 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     7561 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     7621 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     7681 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     7741 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
     7801 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7861 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     7921 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7981 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8041 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
     8101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     8221 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
     8281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8401 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8941 ttgaatactc atattcttcc tttttcaata t
//
