LOCUS       sequence-421004-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF3-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     567..586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(805..896)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     938..957
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     963..982
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1068..1656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1557..1576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1810..1827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1925..1942
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1926..1942
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2398..2414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        2581..2960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2961..3164
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3115..3135
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3161..3185
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3395..3413
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3395..3412
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3426..3445
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6887..6922
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6932..6955
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8615..8659
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8660..8686
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8826..8843)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8832..8943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9061..9081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9066..9395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9246..9381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9308..9327
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9443..9490
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9509..10108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9509..9528)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10005..10025
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10238..10359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10275..10294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10329..10348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10412..10468,1..735,736..804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
       61 ccccagcgct gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat
      121 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
      181 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
      241 caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
      301 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
      361 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
      421 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
      481 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
      541 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
      601 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
      661 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      721 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      781 gacacggaaa tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca
      841 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      901 ggtcagtgtt acaaccaatt aaccaattct gaacattatc gcgagcccat ttatacctga
      961 atatggctca taacacccct tgctcatgac caaaatccct taacgtgagt tacgcgcgcg
     1021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1201 ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa gaactctgta
     1261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1681 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1741 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1801 accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc
     1861 atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct
     1921 gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt
     1981 aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt
     2041 ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga
     2101 gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt
     2161 tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata
     2221 tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg
     2281 aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa
     2341 ctataagatg ttatcagtat ttattatcat ttagaataaa ttttgtgtcg cccttaattg
     2401 tgagcggata acaattacga gcttcatgca cagtggcgtt gactcaatat tggccattag
     2461 ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     2521 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     2581 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2641 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2701 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2761 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2821 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2881 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2941 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     3001 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     3061 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     3121 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     3181 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     3241 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
     3301 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
     3361 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
     3421 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
     3481 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
     3541 cctggtgttc gctgcggccg cccaaagaca agtgacagtg caagagggcc cactgtacag
     3601 aacagagggc agccacatca ccatctggtg caacgtgtcc ggctatcagg gccccagcga
     3661 gcagaatttc cagtggtcca tctacctgcc tagcagcccc gaacgggaag tgcagatcgt
     3721 gtctacaatg gacagcagct tcccctacgc catctacacc cagagagtca gaggcggcaa
     3781 gatcttcatc gagagagtgc agggcaacag caccctgctg cacatcacag atctgcaggc
     3841 cagagatgcc ggcgagtacg agtgtcacac ccctagcacc gacaagcagt acttcggcag
     3901 ctacagcgcc aagatgaacc tggtggtcat ccccgatagc ctgcagacca cagccatgcc
     3961 tcagacactg cacagagtgg aacaggaccc tctggaactg acatgcgagg tggccagcga
     4021 gacaatccag cactctcatc tgtctgtggc ctggctgaga cagaaagtgg gcgagaagcc
     4081 cgtggaagtg atcagcctga gccgggactt tatgctgcac agcagcagcg agtacgccca
     4141 gagacagagt ctgggagaag tgcggctgga caagctgggc agaaccacct tccggctgac
     4201 catcttccat ctgcaaccca gcgatcaggg cgagttctac tgtgaagccg ccgagtggat
     4261 tcaggacccc gatggatctt ggtacgccat gaccagaaag cggagcgaag gcgccgtggt
     4321 caatgtgcag cctaccgaca aagaattcac cgtgcgcctg gaaaccgaga agagactgca
     4381 caccgtgggc gagcctgtgg aattcagatg catcctggaa gcccagaacg tgcccgacag
     4441 atactttgcc gtgtcctggg ccttcaacag cagcctgatt gccacaatgg gccccaatgc
     4501 tgtgcccgtg ctgaatagcg agtttgccca cagagaggcc agaggccagc tgaaggtggc
     4561 caaagaaagc gacagcgtgt tcgtgctgaa gatctaccac ctgagacaag aggacagcgg
     4621 caagtacaac tgcagagtga ccgagcggga aaagaccgtg accggcgagt tcatcgacaa
     4681 agagagcaag cggcccaaga acatccccat catcgtgctg cctctgaagt ccagcatctc
     4741 tgtggaagtg gccagcaacg ccagcgtgat cctcgaaggc gaggacctga gattcagctg
     4801 ttctgtgcgg acagccggca gacctcaggg cagattttct gtgatctggc agctggtgga
     4861 ccggcagaac agacggtcca acatcatgtg gctggacaga gatggcaccg tgcagccagg
     4921 cagcagctac tgggagagaa gctcttttgg cggcgtccag atggaacaag tgcagcccaa
     4981 tagcttcagc ctgggcatct tcaactcccg gaaagaggac gagggccagt acgaatgcca
     5041 cgtgacagag tgggtccgag ccgttgatgg cgagtggcag attgtgggag agagaagggc
     5101 cagcacaccc atctctatca cagccctgga aatgggcttc gccgtgaccg ccattagcag
     5161 aacacctggc gtgacctaca gcgacagctt cgacctgcag tgcatcatca agcctcacta
     5221 ccctgcctgg gtgccagtgt ccgtgacttg gagatttcag cctgtgggca ccgtggaatt
     5281 tcacgacctg gtcaccttca caagagatgg cggagtgcag tggggcgaca gaagcagcag
     5341 ctttagaacc agaaccgcca tcgagaaggc cgagagcagc aacaatgtgc ggctgagcat
     5401 cagcagagcc agcgatacag aggccggaaa gtaccagtgt gtggccgagc tgtggcggaa
     5461 gaactacaac aacacctgga ccagactggc cgagcggacc agcaatctgc tggaaatcag
     5521 agtgctgcag cccgtgacca agctccaggt gtccaagagc aagagaaccc tgacactggt
     5581 ggaaaacaag cccatccagc tgaactgcag cgtgaagtcc cagaccagcc agaacagcca
     5641 tttcgccgtg ctttggtacg tgcacaagcc ctctgatgcc gacggcaagc tgatcctgaa
     5701 aaccacacac aacagcgcct ttgagtacgg cacctacgcc gaggaagaag gccttagagc
     5761 cagactgcag ttcgagcggc atgtgtctgg cggcctgttt tctctgactg tgcagcgggc
     5821 cgaagtgtcc gatagcggca gctattactg ccacgtggaa gaatggctgc tgagccccaa
     5881 ctacgcctgg tataagctgg ccgaagaggt gtccggacgg accgaagtga ctgtgaaaca
     5941 gcccgacagc agactgaggc tgtctcaggc tcagggcaat ctgagcgtgc tggaaacaag
     6001 acaggtgcag ctggaatgcg tggtgctgaa cagaaccagc atcaccagcc agctgatggt
     6061 tgagtggttc gtgtggaagc ctaatcaccc cgagagggaa accgtggcca gactgagtag
     6121 agatgccacc ttccactatg gcgagcaggc cgctaagaac aacctgaagg gcagactgca
     6181 cctggaatca ccctctccag gcgtgtaccg gctgttcatc cagaatgtgg ccgtgcagga
     6241 ctctggcacc tacagctgtc atgtcgaaga gtggctgcct tctccttccg gcatgtggta
     6301 caagagagcc gaggataccg ccggacagac agccctgaca gtgatgaggc ctgatgccag
     6361 cctgcaggtt gacaccgtgg tgcctaatgc caccgtgtct gagaaagccg ccttccagct
     6421 cgactgctcc atcgtgtcca gaagctccca ggacagcaga ttcgccgtgg cctggtacag
     6481 cctgagaaca aaagccggcg gaaagcggtc tagccctggc ctggaagaac aagaggaaga
     6541 gagggaagaa gaagaagaag aggacgacga cgacgatgac gaccccacag aaagaacagc
     6601 cctgctgagc gtgggacccg atgctgtgtt tggacctgag ggctctcctt gggaggggag
     6661 actgagattc cagagactga gccccgtgct gtacagactg acagtgctgc aagcctctcc
     6721 tcaggatacc ggcaactact cctgtcacgt cgaggaatgg ctcccaagtc ctcagaaaga
     6781 gtggtacagg ctgaccgaag aagagagcgc ccctatcggc attcgggtgc tcgatacaag
     6841 ccccacactg cagagcatta tctgcagcaa cgacgctggc gcgccggaag atcaagttga
     6901 tccaagattg attgatggca agggcggatc tctggaggtg ctgttccagg gaccaggcgg
     6961 aagcggcaat cccggcggcg atctggcccc ccagatgctg cgcgagctgc aggagaccaa
     7021 tgccgccctg caggacgtgc gcgagctgct gcgccaccgc gtgaaggaga tcaccttcct
     7081 gaagaacacc gtgatggagt gcgacgcctg cggtatgcag ccctctagag gctccggctc
     7141 catcatccca gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct
     7201 gggtgccgcc aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct
     7261 gggcgatggg atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa
     7321 ggacaaactg gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc
     7381 caagacatac aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct
     7441 gtgcggggtc aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca
     7501 gtgcaacacg acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg
     7561 gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta
     7621 cgcccacacg gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca
     7681 ggaggggtgc caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct
     7741 aggtggaggc cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga
     7801 ctacagccaa ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa
     7861 gcgccagggt gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc
     7921 gtctgtgacc catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg
     7981 agactccaca ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag
     8041 caggaacccc cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca
     8101 tgaaagcagg gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag
     8161 ggcgggccag ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca
     8221 cgtcttctcc ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg
     8281 caaggcccgg gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt
     8341 gctcaaggac ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg
     8401 gcagcagtca gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt
     8461 cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca
     8521 cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc
     8581 cggcaccacc gacgccgcgc acccgggtgg cggaggactg aacgacatct tcgaggctca
     8641 gaaaatcgaa tggcacgaac atcaccatca ccatcaccat caccattaag gccggcctgt
     8701 agtcactggc acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc
     8761 agacagggtt cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa
     8821 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8881 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8941 atcacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9061 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9121 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9241 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9301 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9361 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9421 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9481 ataatacgac aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc
     9541 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     9601 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     9661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     9721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     9781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     9841 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     9901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     9961 cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta
    10021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    10081 gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc
    10141 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10201 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10261 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10321 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10381 ctctagctag agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta
    10441 tctcagcgat ctgtctattt cgttcatc
//
