LOCUS       sequence-421011-        9371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTAB182.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pLNHA-C1-HsTAB182_K
FEATURES             Location/Qualifiers
     source          1..9371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..184
                     /gene="bla"
                     /label=AmpR promoter
     promoter        186..543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        186..377
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(209..229)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      394..529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             578..1372
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             578..1372
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(632..651)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1242..1261
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1560..1579)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1604..1651
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1980..2568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2469..2488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2743..3046
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3047..3250
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3201..3221
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3295..3314
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3295..3313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3341..3350
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3347..3412
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             3437..3463
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8841..8962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8878..8897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8932..8951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(join(8969..9371,1..53))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9106..9127)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9318..9337
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
       61 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      181 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      301 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      361 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      421 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      481 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      541 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      601 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      661 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      721 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
      781 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      841 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      901 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      961 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     1021 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     1081 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     1141 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1201 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1261 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1321 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1381 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1441 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1501 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     1561 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     1621 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     1681 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     1741 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     1801 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     1861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2041 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2101 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2161 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2221 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2281 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2341 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2401 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2461 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2521 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2581 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2641 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     2701 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2761 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2821 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2881 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2941 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3001 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3061 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3121 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3181 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3241 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
     3301 gactcactat agggagaccc aagctctagc gctaccggtc gccaccatgg acgcacaaac
     3361 acgacgacgt gagcgtcgcg ctgagaaaca agctcaatgg aaagctgcaa acggtggatc
     3421 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctca
     3481 agcttcgaat tctgcagtcg acggtaccgc gggcccatca aaagtgtcta ctctcaggga
     3541 aagctcagcc atggcttccc cactgccccg ggagatggag gaggagctgg tgcctactgg
     3601 ctctgagcca ggtgacactc gggccaaacc ccctgtcaag cccaaacccc gggccctgcc
     3661 tgccaagcca gccctgcctg ccaaacccag cctgctggtg cctgttgggc ctcggcctcc
     3721 ccggggtccc ctggctgagt tgccttctgc caggaagatg aacatgctgg caggacccca
     3781 gccctatggt ggcagcaagc gcccccttcc ctttgcacca aggcctgcgg ttgaggcctc
     3841 cactggagga gaagccaccc aagagactgg gaaagaggag gctgggaaag aggagccacc
     3901 ccctttgaca cccccagctc gatgtgcagc cccagggggt gtacggaagg cccctgcccc
     3961 tttccgccca gcctcagagc gcttcgcggc caccacggtg gaagagatcc tggccaagat
     4021 ggagcagcct cggaaggagg tccttgccag ccccgaccgc ctgtggggtt cccgcctcac
     4081 ctttaaccac gatggcagct cgcgatatgg ccccaggacc tatggcacga ccactgctcc
     4141 cagggatgag gatggcagca ccctcttcag gggatggtcc caggaggggc cagtaaagtc
     4201 tccagcagag tgccgggaag agcacagcaa gacccctgag gagaggagcc ttccttccga
     4261 cctggccttc aacggggacc tggctaaggc agccagctcg gagctacctg ctgatatttc
     4321 caagccctgg attccctcaa gtccagcccc ctcctcagag aatggaggcc ctgccagccc
     4381 aggcctcccc gcagaagcct caggctcagg ccctggctct ccccatcttc acccgcctga
     4441 taagagttct ccctgccact cacagcttct ggaagcccag actcctgaag cttcccaggc
     4501 ttctccctgc cccgctgtga ctccatcagc tccaagtgca gccctgcctg acgagggctc
     4561 ccgccacacc cccagcccgg ggctccctgc cgagggggct ccagaggccc ccagacccag
     4621 cagcccaccc cctgaggtct tggagcccca tagcctggat cagccccctg ccacctcacc
     4681 ccggcccctg atcgaggtgg gtgagttgct ggatctcact cggacgtttc catctggcgg
     4741 ggaggaggag gccaagggtg acgcacacct ccgccccacc agcctggttc agcgccgatt
     4801 ctctgaaggt gtgctccagt cacccagtca ggaccaggag aagctggggg gctcgctggc
     4861 tgccctgccc caaggccagg ggagccagtt ggccctggat cgtccctttg gggcagagtc
     4921 caactggagc ttatcacagt ccttcgaatg gaccttcccc acgaggccct cgggtctggg
     4981 cgtgtggcgg ctggactccc cgcctccctc ccccatcact gaagccagtg aggccgccga
     5041 ggctgctgag gctggcaact tggccgtttc cagcagggaa gaaggagtgt ctcagcaggg
     5101 gcaaggggct gggtcagctc caagtgggtc aggaagttcc tgggtgcagg gggatgatcc
     5161 aagcatgtcc ctcacccaga agggcgatgg ggagagtcaa cctcaattcc cagctgttcc
     5221 ccttgagccc ctgcctacaa ctgagggcac acctggatta cctttgcagc aggcagagga
     5281 gagatacgag tcgcaggagc ccttggctgg acaggagtcc cctctccccc tggctaccag
     5341 ggaggcagcc ttgcccatcc tggagccagt cctggggcag gagcagccag cagcccctga
     5401 ccagccctgt gttctctttg ctgatgcccc tgagcctgga caggcactgc ctgttgagga
     5461 ggaggccgtg accctagccc gggctgagac cacccaagcc aggacagagg ctcaagactt
     5521 gtgtagggca tcccccgagc ctccaggccc tgaaagcagc tcccgctggc tggacgacct
     5581 cctggcttca ccaccaccca gtggtggcgg tgcaaggcgg ggagctggag ctgagctgaa
     5641 ggacacacag tccccaagta cctgctctga gggactcctt ggctggtccc agaaagatct
     5701 gcagagtgaa tttgggatca caggagaccc acagcccagc agtttcagtc cttccagctg
     5761 gtgtcaaggt gcttctcagg actatggcct tgggggtgca agccctagag gagacccagg
     5821 tctcggagag agggactgga ccagcaagta tgggcaagga gcaggggaag ggagcaccag
     5881 ggagtgggcc agcaggtgtg gcatcggcca ggaggagatg gaggccagca gcagccaaga
     5941 ccagagtaaa gtgtctgccc caggggtgct cacagcccag gaccgggtag ttggaaagcc
     6001 agcccagctt ggcactcagc ggagccagga ggcagatgtt caggactggg agttcagaaa
     6061 gagggattcc cagggcactt actccagccg ggatgcagaa ctccaggacc aggaattcgg
     6121 aaagagagat tcactgggta cctacagtag tcgagatgta agccttgggg actgggaatt
     6181 tgggaagaga gattctctgg gtgcttatgc cagccaagat gccaacgagc agggccagga
     6241 tttggggaag agggaccacc atggtaggta cagcagccag gatgccgatg agcaggactg
     6301 ggagtttcag aagagagatg tgtcactcgg cacctatggc agccgggctg cggagccaca
     6361 ggaacaggag tttgggaaga gcgcttggat aagggactac agcagtggtg gcagctccag
     6421 gacccttgac gcccaggaca gaagctttgg aacgagaccc ctgagctctg ggttcagccc
     6481 cgaggaagcc cagcaacagg atgaggaatt tgagaagaag attccaagtg tggaagacag
     6541 ccttggagag ggcagcaggg atgctggccg gccaggagag agaggatccg ggggcttgtt
     6601 cagtcctagc actgcccacg tgccggatgg ggcactcggg cagagagacc agagcagctg
     6661 gcaaaacagt gatgctagcc aggaggtggg agggcatcag gagagacagc aggcaggggc
     6721 tcagggccct ggcagtgctg acctggaaga tggggagatg ggaaagcgag gctgggtcgg
     6781 tgagtttagc ctcagtgttg gcccccagcg agaggcagca tttagcccag ggcagcagga
     6841 ctggagccgg gacttctgca tcgaggccag tgagaggagc tatcagtttg gcatcattgg
     6901 caacgacaga gtgagtggtg ctggctttag cccttctagc aagatggaag gtggtcactt
     6961 tgtgcctcct gggaagacca cagctggctc ggtggactgg actgaccagc tgggtctcag
     7021 gaacttggaa gtgtccagct gtgtgggttc tgggggctct agcgaggcca gggagagtgc
     7081 cgtgggacag atgggctggt caggtggcct gagcttgaga gacatgaacc tgaccggctg
     7141 tttggaaagt ggagggtctg aagagccggg gggaatcgga gttggggaga aggactggac
     7201 ttctgatgtt aatgtgaaga gcaaagattt ggctgaggtc ggggagggag gaggccacag
     7261 ccaggccaga gagagtggcg tggggcagac tgactggtca ggtgtggagg ccggagagtt
     7321 ccttaaatca agggagcgtg gagttggaca ggcagactgg acacctgacc ttgggctgag
     7381 aaacatggcc ccaggggcag tctgcagtcc tggagagtcc aaagagcttg gggtgggcca
     7441 gatggactgg ggtaacaatc tgggcctgag ggatttggag gtgacctgtg acccagactc
     7501 tggaggttct caggggctac ggggatgtgg agtggggcag atggactgga cccaggactt
     7561 ggcgccccag aatgtggagc tctttggggc tccaagtgaa gccagggagc atggggtggg
     7621 cggggtgagc cagtgcccag agcccggcct gaggcacaat ggcagcttgt ctcctggcct
     7681 ggaggccaga gaccccttgg aggccaggga gctgggggtt ggtgagacaa gtgggccaga
     7741 gacccagggt gaagattact cctcgtcttc cttggagcca caccctgcag accctggaat
     7801 ggagacagga gaagccctca gcttcggagc aagccctggc aggtgcccgg cccgcccccc
     7861 accctccggc tcccagggcc tgctggagga gatgctggca gccagcagct ccaaggcggt
     7921 ggctcggagg gagtcagcgg cctcgggcct tgggggcctg ttggaggagg aaggagccgg
     7981 ggcaggtgct gcccaagagg aggtgctgga gcctggcagg gactctccac cctcctggag
     8041 gccgcagcct gatggtgagg ccagccagac agaagacgtg gatggcacct ggggctcttc
     8101 agcagccagg tggagcgatc aggggccagc acagacttct cggcgaccct cccaaggccc
     8161 tcctgccaga tcccccagtc aggacttctc cttcattgag gacaccgaga tcctcgacag
     8221 tgccatgtat cggagccgtg ccaacttggg gcgcaagcgt gggcaccggg ccccggtcat
     8281 tcggcctggg ggtaccttgg gcctgtcgga ggcagcagac tcggatgcac acctgttcca
     8341 ggactctaca gagccacggg catctcgggt gccatcttca gatgaagagg tagtggagga
     8401 acctcagagc cgccggacac ggatgtcgtt gggcaccaag gggctgaaag tcaacctctt
     8461 tcctggcctg agcccctcag ccctgaaggc caagctgcgc ccccggaatc gctcagctga
     8521 ggagggagag ctggctgaga gcaagtcgag ccagaaggag tccgcggtcc agcgttcgaa
     8581 atcctgcaag gtcccaggac tgggaaagcc cctcacgtta cctcccaagc cagagaaatc
     8641 ctcagggtca gaaggatcgt cgcccaactg gcttcaagcc ctgaaactga agaagaagaa
     8701 ggtctgagat gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     8761 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     8821 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     8881 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8941 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     9001 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     9061 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     9121 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     9181 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     9241 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     9301 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     9361 gtagcggtca c
//
