LOCUS       sequence-42102-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: nGCaMP2-NpuDnaEn
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..72
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(76..84,85..99)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..183
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     396..417
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccaccatgc ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
       61 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac catggtcgac
      121 tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc
      181 tcactcgaga acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc
      241 aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
      301 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      361 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      421 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggatcctg tttaagctat
      481 gaaacggaaa tattgacagt agaatatgga ttattaccga ttggtaaaat tgtagaaaag
      541 cgcatcgaat gtactgttta tagcgttgat aataatggaa atatttatac acaacctgta
      601 gcacaatggc acgatcgcgg agaacaagag gtgtttgagt attgtttgga agatggttca
      661 ttgattcggg caacaaaaga ccataagttt atgactgttg atggtcaaat gttgccaatt
      721 gatgaaatat ttgaacgtga attggatttg atgcgggttg ataatttgcc gaattag
//
