LOCUS       sequence-421023-        8538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LR vector for integration of Gse1-exon into N2a FRT rtTA3 expression
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Roth JF, Braunschweig U, Wu M, Li JD, Lin ZY, Larsen B, Weatheritt 
            RJ, Gingras AC, Blencowe BJ
  TITLE     Systematic analysis of alternative exon-dependent interactome 
            remodeling reveals multitasking functions of gene regulatory 
            factors.
  JOURNAL   Mol Cell. 2023 Dec 7;83(23):4222-4238.e10. doi: 
            10.1016/j.molcel.2023.10.034.
  PUBMED    38065061
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.10"; journalName: "Mol Cell"; date: 
            "2023-12-7- 7"; volume: "83"; issue: "23"; pages: "4222-4238.e10"
COMMENT     Sequence Label: 5'-3xFLAG Gse1-exon minimal CMV pCDNA5
FEATURES             Location/Qualifiers
     source          1..8538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    130..177
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             185..1207
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1337..1458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1374..1393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1428..1447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1507..1523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1507..1523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1520..1542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1531..1547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1555..1561,1562..1579,1580..1585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1600..1621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1738..1755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1909..2497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1989..2008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2668..3459,3460..3528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3291..3310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3529..3633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3708..3727)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    3926..3944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3963..3981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4000..4018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4037..4055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4074..4092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4111..4129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4148..4166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4185..4203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4222..4240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4259..4277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4299..4323
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4475..4498
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4499..4523
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6498..6518
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(8088..8112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8154..8171)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8160..8384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8517..8536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
       61 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      121 acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt
      181 ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
      241 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
      301 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
      361 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
      421 tggggagttc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
      481 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
      541 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
      601 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
      661 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
      721 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
      781 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
      841 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
      901 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
      961 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     1021 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     1081 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     1141 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     1201 ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1261 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1321 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1441 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1501 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1561 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1621 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1681 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1741 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1801 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1861 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1921 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1981 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2041 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2101 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2161 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2221 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2281 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2341 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2401 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2461 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2521 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2581 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2641 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2701 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2761 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2821 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2881 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2941 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3001 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3061 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3121 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3181 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3241 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3301 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3361 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3421 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3481 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3541 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3601 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3661 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3721 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3781 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3841 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtctc
     3901 gagggatcca agctggccgc acgtctccct atcagtgata gagaagtcga cacgtctcga
     3961 gctccctatc agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag
     4021 tcgacacgtc tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat
     4081 cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta
     4141 gaacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag
     4201 agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc
     4261 cctatcagtg atagagaagg taccccctat ataagcagag ctcgtttagt gaaccgtcag
     4321 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     4381 agcctccgcg gaggctggat cctctagcgt ttaaacttaa gcttccacca tggactacaa
     4441 agaccatgac ggtgatacat atgatcatga catcgattac aaggatgacg atgacaagac
     4501 aagtttgtac aaaaaagttg gcaccttcgg actgaagccg cctctctatt acttaccagg
     4561 catgagccat gagcccaagt ccccttccat agggatgctt tccacggcga ccaggaccac
     4621 cgccaccgtc aaccccctca ccccctcgcc actcaatggc gccctggtgc ccaccggtag
     4681 cccggccacc agcagtacgc tgtcggccca ggccgctcca tcctccagct ttgctgccgc
     4741 actgcgcaag ctcgccaaac aggcagagga gcccagaggg tcctcactga gcagcgagtc
     4801 atctccagtg tcctctccag ccaccaacca tagctctcca gctagcacgc ccaaacgagt
     4861 gcccatgggc cccatcatcg tcccccccgg gggtcacagt gtccccagca ctcccccagt
     4921 ggtcactatt gcccccacta aaaccgtcaa tggagtctgg agaagcgaga gccgccagga
     4981 ctctggctca cgaggcagca gcagtggtcg ggagcgccta ttggtggagc cacctctcgc
     5041 ccaggaaaag gctgcgggcc ccgccatccc ctcccaccta ctcagcaccc cgtacccctt
     5101 tggcctttcc cccggctcag ttgtacagga ctcccgcttc caaccactca acctccagcg
     5161 gcctgtgcac catgtggtgc cccccagcac ggtgactgag gactacttga gaagcttccg
     5221 gccctaccac actgctgaag acttacgcat gtcctccttg cctccgcttg gcctggaccc
     5281 agccaccgcc gcagcctact atcaccccag ctacctggcc ccacacccat tcccccaccc
     5341 ggccttcagg atggatgact cctattgcct gtcagcctta aggtctccgt tctaccccat
     5401 ccccacccct ggctccctgc ctccactgca tccgtcggcg atgcacctgc atctctccgg
     5461 cgtacggtat cctcctgagc tctcccactc gtccctggcg gcgctacact cggagcggat
     5521 gtccagcctc agtgcagaga ggttgcaaat ggacgaggaa ctgcggcggg agagagagcg
     5581 agagcgggaa cgggaacggg aggccgaccg ggagcgggag aaggagcggg agcgggagca
     5641 gcgggagaag gagcgggaga aggagctgga gcgcgagcgg gagaaggaac gggagcgcga
     5701 gctggagcgg cagcgggagc agcgggcgag ggagaaggag ctgctggctg ccaaggcctt
     5761 agagcccacc accttcctgc ctgtggccga gctgcacgga ctccgaggtc acagcacgga
     5821 ggagcggccc aagccctcgg agcagctgac cccaacccga gcagagaagc tgaaggacgt
     5881 gggcttgcag gcgcccaagc ccgtgcagca ccctctgcac ccagtgcctg ccccacacca
     5941 cacggtgccc agcctcatct ccagccacgg catcttctct ctgcctggaa gcagcgccac
     6001 caccgccctg ctgatccagc gcaccaacga ggaggagaag tggctggcac ggcagcgccg
     6061 cctgcggcag gagaaggaag accgccagtc ccaggtgtcc gagttccggc agcaggtcct
     6121 ggagcagcac ctagacctgg gccggcccct ggtgcccaca gaggcggagc acaggcccga
     6181 gagcaccagg ccaggaacaa accggcatga gcagggcagc cgtgaacccc cacagcactt
     6241 tggtggccct ccacccctca tctcacccaa gccccagcag cacaccgtac ccacagccct
     6301 ctggaaccca gtgtctctga tggacaatgc tctggagacg cggcgggccg agagccactc
     6361 tctgcatagc cacccgactg cttttgagcc cagccgccag gccgccgtgc cactggtgaa
     6421 ggtggagcga gtttattgct cagagaaggc agaggagccc cggaagcgtg aggccacacc
     6481 actggacaaa taccagccac caccaccacc accaccaccg cgggaggcag gaagtctgga
     6541 gcctcagacc tttccccacg gacctgggcc tttccttact gaacttgaaa agtcaacaca
     6601 gaccatcttg ggccagcagc ggccctctct ttcccaggca acctccttcg gggagctcag
     6661 tgggcctctg aaaccaggct ctccgtactg ccaccccaca gcgaggggcc ccgatccagc
     6721 ctacatctac gatgagtttc ttcagcagcg acggaagctg gtcagcaaac tggacctgga
     6781 ggaacgcagg cggcgcgagg cccaggagaa agggtactac tacgacctcg atgactcgta
     6841 cgacgagagt gacgaggagg aggtcagggc acacctccgc tgtgtggctg agcagccgcc
     6901 cctcaaactg gacacatcct cggagaagct agagttcttg caactttttg gcttgaccac
     6961 ccaacagcag aaggaggaac tggtggcgca gaagcgccgg aagcggaggc ggatgctcag
     7021 agagagaagc ccgtcgccac ctgccgttca gtgcaagcga cagacgcctt cccccaggct
     7081 ggctctgtcc acccgctaca gccccgacga gatgaacaac agccccaact ttgaggagaa
     7141 gaggaagttc ctgaccttct tcaacttgac gcacatcagt gcggagaaga ggaaagaacc
     7201 agccacacag ccagctcctc tagagacgga tcagcctgtc ggggtccctg cctccttgtc
     7261 agatgtccca aagaccacgg agaccgggag actggaacag ctccggcccc aggagctctt
     7321 gagagtccag gagccggctc ctcccagtgg tgagaaggcc aggctgagcg aggcccctgg
     7381 tggcaagaag agcctgagca tgctccatta cctccggggc gccgcgccca aggacattcc
     7441 tgtgccgttg tctcacagca tcaacgggaa gagcaagccc tgggagcctt tcgtggccga
     7501 agagtttgca catcaattcc acgagtccgt gctgcagtcc acacagaagg ctctgcagaa
     7561 gcataaaggg aactctgctt tgctatctgc agagcagagc cacaaagtcg acacggcaat
     7621 ccactacaac attcccgagc tgcagtcctc cagcagggtc cccttgcccc agcacaatgg
     7681 acaacaggag cctccaatgg ggaggaaggg ccctcctatg caggaggcgg accaggactc
     7741 tgaggaggac tctgaggagg acagcgagga ggaagctgag gaagccccga ggcgccagtg
     7801 gcaagggatt gaggcaatct ttgaagctta ccaggaacac atagaagagc aaaacctgga
     7861 gcggcaggtg ctgcagacgc agtgccggcg gttggaggcg cagaactaca gcctcagcct
     7921 gactgcggag cagctgtctc acagcatggc ggagcttcgg agtcagaaac agaagatggt
     7981 ctctgagcgg gagcggctcc aggcggagct ggaccacttg cggaagtgcc ttgccttgcc
     8041 caccatgcac tggcctaggg gctactttaa gggatatccg aggtgatacc caactttctt
     8101 gtacaaagtg gtctgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     8161 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8221 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8281 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8341 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     8401 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     8461 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8521 tttcgctttc ttcccttc
//
