LOCUS       sequence-421036-       10824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian-cell-based plasmid system that expresses ECFP (a non 
            R-loop-forming transcript) upon dox-induction, which will further 
            cause co-directional transcription-replication conflict (CD TRC)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA
  TITLE     Transcription-Replication Conflict Orientation Modulates R-Loop 
            Levels and Activates Distinct DNA Damage Responses.
  JOURNAL   Cell. 2017 Aug 10;170(4):774-786.e19. doi: 
            10.1016/j.cell.2017.07.043.
  PUBMED    28802045
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "774-786.e19"
COMMENT     Sequence Label: ECFP CD
FEATURES             Location/Qualifiers
     source          1..10824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      462..2251
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2553..4478)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGXGGXGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGXGXGGGAGXGGGXGGAGXGGGXGGXGGXGAGGGXGXGGXGGXGGXGXGGAGAGG
                     GAGGAGGXGAGGAGXGGXGXGGXGXGGXGGXGXGGXGGXGXGGXGGXGXGGGXGGXGXG
                     GGXGGXGXGGXGGXGXGGXGGXGXGGXGGXGXGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4913..4931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4999..5103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5104..5172,5173..5964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5322..5341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6135..6723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6624..6643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7279..7424
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7467..8504
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8502..8521)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8546..8594
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9108..9130
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    9273..9407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9310..9329)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9364..9383
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9440..10150,10151..10153,10154..10156))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9482..9503)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9831..9850
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10090..10111
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      10153..10162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10186..10500)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(10189..10213)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10260..10278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10296..10314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10331..10349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10367..10385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10403..10421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10438..10456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10474..10492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
       61 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
      121 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
      181 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
      241 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
      301 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
      361 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
      421 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
      481 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
      541 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
      601 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
      661 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
      721 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
      781 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
      841 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
      901 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
      961 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     1021 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     1081 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     1141 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     1201 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     1261 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     1321 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     1381 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     1441 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     1501 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     1561 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     1621 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     1681 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     1741 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     1801 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     1861 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     1921 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     1981 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     2041 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     2101 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     2161 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     2221 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     2281 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     2341 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     2401 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     2461 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     2521 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     2581 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     2641 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     2701 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     2761 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     2821 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     2881 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     2941 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     3001 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     3061 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     3121 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     3181 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     3241 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     3301 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     3361 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     3421 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     3481 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     3541 sctcctsccc ctcctscccc tcctsctcct scccctccts cccctcctsc tcctscccct
     3601 cctscccctc ctsctcctsc ccctcctscc cctcctccts ctcctscccc tcctscccct
     3661 cctcctsctc ctscccctcc tscccctcct sctcctsccc ctcctscccc tcctsctcct
     3721 scccctccts cccctcctsc tcctscccct cctsctccts cccctcctsc tcctgcccct
     3781 cctgctccts cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctsctcct
     3841 scccctccts cccctcctsc ccctcctsct cctscccctc ctcctgctcc tscccctcct
     3901 scccctccts cccctcctcc tsctcctgcc cctcctsccc ctcctcctsc tcctgcccct
     3961 cctcctsctc ctscccctcc tscycctcct scccctcctc ctsctcctsc ccctcctscc
     4021 cctcctccts ctcctscccc tcctcctsct cctscccctc ctscycctcc tscccctcct
     4081 cctsctccts cccctcctcc tsctcctscc cctcctsccc ctcctscccc tcctgcccct
     4141 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     4201 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     4261 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     4321 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     4381 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     4441 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     4501 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     4561 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
     4621 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     4681 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     4741 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     4801 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     4861 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     4921 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5341 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5641 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6241 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     6781 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     6841 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cggatctcat
     6901 ggtgctgtat ataaaaccag tggttatatg tacagtagct gtatataaaa ccagtggtta
     6961 tatgtacagt agctgtatat aaaaccagtg gttatatgta cagtagtacg ttagatccga
     7021 acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga
     7081 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     7141 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc
     7201 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
     7261 catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg
     7321 aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg
     7381 cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg
     7441 ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg
     7501 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     7561 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7621 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7681 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7741 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7801 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7861 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7921 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7981 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     8041 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     8101 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     8161 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     8221 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     8281 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     8341 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     8401 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     8461 gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca
     8521 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
     8581 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     8641 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     8701 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     8761 cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg
     8821 gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca
     8881 tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt
     8941 ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa
     9001 gctagtcgac ctcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     9061 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9121 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagccggatc ataatcagcc
     9181 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     9241 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     9301 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9361 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgg ccttgccggc
     9421 ctcgagatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     9481 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     9541 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     9601 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     9661 agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgatgt
     9721 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     9781 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     9841 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     9901 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccccagg
     9961 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
    10021 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
    10081 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
    10141 cgcccttgct caccatggtg gcgggtaccg agctcgaatt ctccaggcga tctgacggtt
    10201 cactaaacga gctctgctta tataggcctc ccaccgtaca cgcctacctc gacatacgtt
    10261 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggataaact
    10321 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
    10381 agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg acatcgttct
    10441 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
    10501 gtgtacatat tatgatatag atacaacgta tgcaatggcc aatagccaat attgatttat
    10561 gctatataac caatgactaa tatggctaat tgccaatatt gattcaatgt atagatctca
    10621 tggtgctgta tataaaacca gtggttatat gtacagtagc tgtatataaa accagtggtt
    10681 atatgtacag tagctgtata taaaaccagt ggttatatgt acagtagtac gttagatctt
    10741 ccatacctac cagttctgcg cctgcagcaa tggcaacaac gttgcccgga tctgcgatga
    10801 taagctgtca aacatgagaa ttgg
//
