LOCUS       sequence-421050-       17619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17619)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 17619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLCRISPR/Cas9Pubi-H
FEATURES             Location/Qualifiers
     source          1..17619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             730..750
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             775..4875
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             775..4875
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             1961..1969
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4876..4923
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4936..5188
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5306..5327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5342..5347,5348..5365,5366..5372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5380..5396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5438..5456
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5438..5455
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5530..5832
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5711..5730
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    6042..6075
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6119..6136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6119..6135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6128..6150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6338..6362
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7663..8292
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8721..9794
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9860..10054
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10290..10312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10398..10538
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10453..10472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10553..10570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10724..11312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10804..10823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11399..12193)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13817..13841
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13919..14093
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(14134..15159)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(15226..15902)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15269..15289)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    16093..16114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16129..16134,16135..16152,16153..16159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16167..16183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16191..16207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat
       61 gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag
      121 tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc
      181 atacatattc atagttacga attgaagatg atggatggaa atatcgatct aggataggta
      241 tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg
      301 tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt
      361 tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat
      421 agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg
      481 gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg
      541 agtacctatc tattataata aacaagtatg ttttataatt attttgatct tgatatactt
      601 ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat
      661 ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg
      721 cagatggctc ctaagaagaa gcggaaggtt ggtattcacg gggtgcctgc ggctgacaag
      781 aagtactcca tcggcctcga catcggcacc aacagcgtcg gctgggcggt gatcaccgac
      841 gagtacaagg tcccgtccaa gaagttcaag gtcctgggca acaccgaccg ccactccatc
      901 aagaagaacc tcatcggcgc cctcctcttc gactccggcg agacggcgga ggcgacccgc
      961 ctcaagcgca ccgcccgccg ccgctacacc cgccgcaaga accgcatctg ctacctccag
     1021 gagatcttct ccaacgagat ggcgaaggtc gacgactcct tcttccaccg cctcgaggag
     1081 tccttcctcg tggaggagga caagaagcac gagcgccacc ccatcttcgg caacatcgtc
     1141 gacgaggtcg cctaccacga gaagtacccc actatctacc accttcgtaa gaagcttgtt
     1201 gactctactg ataaggctga tcttcgtctc atctaccttg ctctcgctca catgatcaag
     1261 ttccgtggtc acttccttat cgagggtgac cttaaccctg ataactccga cgtggacaag
     1321 ctcttcatcc agctcgtcca gacctacaac cagctcttcg aggagaaccc tatcaacgct
     1381 tccggtgtcg acgctaaggc gatcctttcc gctaggctct ccaagtccag gcgtctcgag
     1441 aacctcatcg cccagctccc tggtgagaag aagaacggtc ttttcggtaa cctcatcgct
     1501 ctctccctcg gtctgacccc taacttcaag tccaacttcg acctcgctga ggacgctaag
     1561 cttcagctct ccaaggatac ctacgacgat gatctcgaca acctcctcgc tcagattgga
     1621 gatcagtacg ctgatctctt ccttgctgct aagaacctct ccgatgctat cctcctttcg
     1681 gatatcctta gggttaacac tgagatcact aaggctcctc tttctgcttc catgatcaag
     1741 cgctacgacg agcaccacca ggacctcacc ctcctcaagg ctcttgttcg tcagcagctc
     1801 cccgagaagt acaaggagat cttcttcgac cagtccaaga acggctacgc cggttacatt
     1861 gacggtggag ctagccagga ggagttctac aagttcatca agccaatcct tgagaagatg
     1921 gatggtactg aggagcttct cgttaagctt aaccgtgagg acctccttag gaagcagagg
     1981 actttcgata acggctctat ccctcaccag atccaccttg gtgagcttca cgccatcctt
     2041 cgtaggcagg aggacttcta ccctttcctc aaggacaacc gtgagaagat cgagaagatc
     2101 cttactttcc gtattcctta ctacgttggt cctcttgctc gtggtaactc ccgtttcgct
     2161 tggatgacta ggaagtccga ggagactatc accccttgga acttcgagga ggttgttgac
     2221 aagggtgctt ccgcccagtc cttcatcgag cgcatgacca acttcgacaa gaacctcccc
     2281 aacgagaagg tcctccccaa gcactccctc ctctacgagt acttcacggt ctacaacgag
     2341 ctcaccaagg tcaagtacgt caccgagggt atgcgcaagc ctgccttcct ctccggcgag
     2401 cagaagaagg ctatcgttga cctcctcttc aagaccaacc gcaaggtcac cgtcaagcag
     2461 ctcaaggagg actacttcaa gaagatcgag tgcttcgact ccgtcgagat cagcggcgtt
     2521 gaggaccgtt tcaacgcttc tctcggtacc taccacgatc tcctcaagat catcaaggac
     2581 aaggacttcc tcgacaacga ggagaacgag gacatcctcg aggacatcgt cctcactctt
     2641 actctcttcg aggataggga gatgatcgag gagaggctca agacttacgc tcatctcttc
     2701 gatgacaagg ttatgaagca gctcaagcgt cgccgttaca ccggttgggg taggctctcc
     2761 cgcaagctca tcaacggtat cagggataag cagagcggca agactatcct cgacttcctc
     2821 aagtctgatg gtttcgctaa caggaacttc atgcagctca tccacgatga ctctcttacc
     2881 ttcaaggagg atattcagaa ggctcaggtg tccggtcagg gcgactctct ccacgagcac
     2941 attgctaacc ttgctggttc ccctgctatc aagaagggca tccttcagac tgttaaggtt
     3001 gtcgatgagc ttgtcaaggt tatgggtcgt cacaagcctg agaacatcgt catcgagatg
     3061 gctcgtgaga accagactac ccagaagggt cagaagaact cgagggagcg catgaagagg
     3121 attgaggagg gtatcaagga gcttggttct cagatcctta aggagcaccc tgtcgagaac
     3181 acccagctcc agaacgagaa gctctacctc tactacctcc agaacggtag ggatatgtac
     3241 gttgaccagg agctcgacat caacaggctt tctgactacg acgtcgacca cattgttcct
     3301 cagtctttcc ttaaggatga ctccatcgac aacaaggtcc tcacgaggtc cgacaagaac
     3361 aggggtaagt cggacaacgt cccttccgag gaggttgtca agaagatgaa gaactactgg
     3421 aggcagcttc tcaacgctaa gctcattacc cagaggaagt tcgacaacct cacgaaggct
     3481 gagaggggtg gcctttccga gcttgacaag gctggtttca tcaagaggca gcttgttgag
     3541 acgaggcaga ttaccaagca cgttgctcag atcctcgatt ctaggatgaa caccaagtac
     3601 gacgagaacg acaagctcat ccgcgaggtc aaggtgatca ccctcaagtc caagctcgtc
     3661 tccgacttcc gcaaggactt ccagttctac aaggtccgcg agatcaacaa ctaccaccac
     3721 gctcacgatg cttaccttaa cgctgtcgtt ggtaccgctc ttatcaagaa gtaccctaag
     3781 cttgagtccg agttcgtcta cggtgactac aaggtctacg acgttcgtaa gatgatcgcc
     3841 aagtccgagc aggagatcgg caaggccacc gccaagtact tcttctactc caacatcatg
     3901 aacttcttca agaccgagat caccctcgcc aacggcgaga tccgcaagcg ccctcttatc
     3961 gagacgaacg gtgagactgg tgagatcgtt tgggacaagg gtcgcgactt cgctactgtt
     4021 cgcaaggtcc tttctatgcc tcaggttaac atcgtcaaga agaccgaggt ccagaccggt
     4081 ggcttctcca aggagtctat ccttccaaag agaaactcgg acaagctcat cgctaggaag
     4141 aaggattggg accctaagaa gtacggtggt ttcgactccc ctactgtcgc ctactccgtc
     4201 ctcgtggtcg ccaaggtgga gaagggtaag tcgaagaagc tcaagtccgt caaggagctc
     4261 ctcggcatca ccatcatgga gcgctcctcc ttcgagaaga acccgatcga cttcctcgag
     4321 gccaagggct acaaggaggt caagaaggac ctcatcatca agctccccaa gtactctctt
     4381 ttcgagctcg agaacggtcg taagaggatg ctggcttccg ctggtgagct ccagaagggt
     4441 aacgagcttg ctcttccttc caagtacgtg aacttcctct acctcgcctc ccactacgag
     4501 aagctcaagg gttcccctga ggataacgag cagaagcagc tcttcgtgga gcagcacaag
     4561 cactacctcg acgagatcat cgagcagatc tccgagttct ccaagcgcgt catcctcgct
     4621 gacgctaacc tcgacaaggt cctctccgcc tacaacaagc accgcgacaa gcccatccgc
     4681 gagcaggccg agaacatcat ccacctcttc acgctcacga acctcggcgc ccctgctgct
     4741 ttcaagtact tcgacaccac catcgacagg aagcgttaca cgtccaccaa ggaggttctc
     4801 gacgctactc tcatccacca gtccatcacc ggtctttacg agactcgtat cgacctttcc
     4861 cagcttggtg gtgataagcg tcctgctgcc accaaaaagg ccggacaggc taagaaaaag
     4921 aagtaggatc ctcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     4981 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     5041 attaacatgt aatgcatgac gttatttatg aggtgggttt ttatgattag agtcccgcaa
     5101 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     5161 cgcgcggtgt catctatgtt actagatcgg gagcaccggt aaggcgcgcc gtagtgctcg
     5221 agagacctct gaagtggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5281 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5341 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5401 acacaagaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     5461 tcaagctatg catcaagctc aatgggtcta gtctgtagat acccatcaca ctggcgaccg
     5521 ctcgaacatc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     5581 gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt
     5641 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     5701 gaaagctggc gcatgatgac caccgatatg gccagtgtgc ctgtctccgt tatcggggaa
     5761 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     5821 tggggaatat aaatgtcagg cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     5881 gcgcggcggg tgagcgtggg tctcgcggta tcattggcgc gcctctcgag ctagcggccg
     5941 catgcatcga tctcctacat cgtataaatt agcctatacg aagttattgc atctatgtcg
     6001 ggtgcggaga aagaggtaat gaaatggcag tattagatct gataacttcg tataatgtat
     6061 gctatacgaa gttatgactg caggtcgaca cccataatag ctgtttgcca agcttggcac
     6121 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6181 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6241 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
     6301 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     6361 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     6421 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     6481 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     6541 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     6601 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     6661 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     6721 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     6781 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     6841 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     6901 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     6961 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     7021 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     7081 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     7141 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     7201 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     7261 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     7321 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     7381 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     7441 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     7501 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     7561 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     7621 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     7681 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     7741 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     7801 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     7861 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     7921 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     7981 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     8041 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     8101 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     8161 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     8221 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     8281 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     8341 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     8401 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     8461 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     8521 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     8581 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     8641 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
     8701 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     8761 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     8821 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     8881 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     8941 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     9001 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     9061 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     9121 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     9181 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     9241 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     9301 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     9361 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     9421 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     9481 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     9541 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     9601 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     9661 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     9721 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     9781 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     9841 gtcgaaaagt tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     9901 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     9961 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
    10021 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
    10081 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
    10141 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
    10201 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
    10261 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10381 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
    10441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    10501 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    10561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
    11401 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    11461 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    11521 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    11581 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    11641 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    11701 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    11761 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    11821 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    11881 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    11941 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    12001 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    12061 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    12121 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    12181 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    12241 agctaaggaa ggtgcgaaca agtccctgat atgagatcat gtttgtcatc tggagccata
    12301 gaacagggtt catcatgagt catcaactta ccttcgccga cagtgaattc agcagtaagc
    12361 gccgtcagac cagaaaagag attttcttgt cccgcatgga gcagattctg ccatggcaaa
    12421 acatggtgga agtcatcgag ccgttttacc ccaaggctgg taatggccgg cgaccttatc
    12481 cgctggaaac catgctacgc attcactgca tgcagcattg gtacaacctg agcgatggcg
    12541 cgatggaaga tgctctgtac gaaatcgcct ccatgcgtct gtttgcccgg ttatccctgg
    12601 atagcgcctt gccggaccgc accaccatca tgaatttccg ccacctgctg gagcagcatc
    12661 aactggcccg ccaattgttc aagaccatca atcgctggct ggccgaagca ggcgtcatga
    12721 tgactcaagg caccttggtc gatgccacca tcattgaggc acccagctcg accaagaaca
    12781 aagagcagca acgcgatccg gagatgcatc agaccaagaa aggcaatcag tggcactttg
    12841 gcatgaaggc ccacattggt gtcgatgcca agagtggcct gacccacagc ctggtcacca
    12901 ccgcggccaa cgagcatgac ctcaatcagc tgggtaatct gctgcatgga gaggagcaat
    12961 ttgtctcagc cgatgccggc taccaagggg cgccacagcg cgaggagctg gccgaggtgg
    13021 atgtggactg gctgatcgcc gagcgccccg gcaaggtaag aaccttgaaa cagcatccac
    13081 gcaagaacaa aacggccatc aacatcgaat acatgaaagc cagcatccgg gccagggtgg
    13141 agcacccatt tcgcatcatc aagcgacagt tcggcttcgt gaaagccaga tacaaggggt
    13201 tgctgaaaaa cgataaccaa ctggcgatgt tattcacgct ggccaacctg tttcgggcgg
    13261 accaaatgat acgtcagtgg gagagatctc actaaaaact ggggataacg ccttaaatgg
    13321 cgaagaaacg gtctaaatag gctgattcaa ggcatttacg ggagaaaaaa tcggctcaaa
    13381 catgaagaaa tgaaatgact gagtcagccg agaagaattt ccccgcttat tcgcaccttc
    13441 cctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    13501 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    13561 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    13621 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    13681 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    13741 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    13801 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    13861 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
    13921 tctggatttt agtactggat tttggtttta ggaattagaa attttattga tagaagtatt
    13981 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
    14041 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
    14101 ttgtcgatcg acagatcccg gtcggcatct actctatttc tttgccctcg gacgagtgct
    14161 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
    14221 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
    14281 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
    14341 ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg
    14401 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
    14461 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
    14521 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
    14581 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
    14641 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
    14701 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
    14761 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg
    14821 ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgaacg
    14881 gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat
    14941 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
    15001 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
    15061 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
    15121 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccggatct
    15181 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
    15241 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
    15301 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
    15361 aacgtcttct ttttccacga tgctcttcgt gggtgggggt ccatctttgg gaccactgtc
    15421 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    15481 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    15541 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    15601 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
    15661 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    15721 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    15781 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    15841 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    15901 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    15961 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
    16021 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    16081 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    16141 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    16201 ctatgacatg attacgaatt cgagctcggt accgtaacta taacggtcct aaggtagcga
    16261 aggatccgct cgctacctta agagaggata tccctccatc ctataatgta ggctatagga
    16321 actagggcaa ggccggccat gcggccgcaa gctgggtgca gcgtgacccg gtcgtgcccc
    16381 tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt
    16441 gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta
    16501 cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga
    16561 acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta
    16621 tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact
    16681 tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat
    16741 ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta
    16801 ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa
    16861 ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt
    16921 agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca
    16981 gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga
    17041 cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg
    17101 cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca
    17161 ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
    17221 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
    17281 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
    17341 gctcgtcctc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
    17401 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
    17461 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
    17521 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
    17581 atttcatgat tttttttgtt tcgttgcata gggtttggt
//
