LOCUS       sequence-421053-        7992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses truncated BID upon doxycycline addition to trigger 
            apoptosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Nam M, Xia W, Mir AH, Jerrett A, Spinelli JB, Huang TT, Possemato R
  TITLE     Glucose limitation protects cancer cells from apoptosis induced by 
            pyrimidine restriction and replication inhibition.
  JOURNAL   Nat Metab. 2024 Dec;6(12):2338-2353. doi: 
            10.1038/s42255-024-01166-w. Epub 2024 Nov 26.
  PUBMED    39592843
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-024-01166-w"; journalName: "Nat Metab"; date: 
            "2024-12"; volume: "6"; issue: "12"; pages: "2338-2353"
COMMENT     Sequence Label: pCW57.1 blast tBID-flag
FEATURES             Location/Qualifiers
     source          1..7992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             966..989
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1017..1073
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        1101..1611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1530..1548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1640..2035
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2051..2104
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2105..2722,2723..2728,2729..2764,2765..2767,2768..2803,
                     2804..2806,2807..2842,2843..2851)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2891..2911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3309..3320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3525..3758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3830..3951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3867..3886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3921..3940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3991..4126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4126..4197
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4198..4266,4267..5058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4416..4435)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5229..5817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5718..5737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5971..5988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6105..6126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6141..6146,6147..6164,6165..6171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6179..6195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6184..6206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6203..6219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6203..6219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6238..6258
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6240..6258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6286..6512
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6513..6693
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6740..6865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7358..7591
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7776..7820
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcaccatggg caaccgcagc agccactccc gcttgggaag aatagaggca gattctgaaa
      601 gtcaagaaga catcatccgg aatattgcca ggcacctcgc ccaggtcggg gacagcatgg
      661 accgtagcat ccctccgggc ctggtgaacg gcctggccct gcagctcagg aacaccagcc
      721 ggtcggagga ggaccggaac agggacctgg ccactgccct ggagcagctg ctgcaggcct
      781 accctagaga catggagaag gagaagacca tgctggtgct ggccctgctg ctggccaaga
      841 aggtggccag tcacacgccg tccttgctcc gtgatgtctt tcacacaaca gtgaatttta
      901 ttaaccagaa cctacgcacc tacgtgagga gcttagccag aaatgggatg gacgccggag
      961 gagccgatta caaggacgac gatgacaagt gagaattcgt cgacaccggt tccggagcca
     1021 cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt cctaggctgc
     1081 agacgcgttg tacaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     1141 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     1201 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     1261 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     1321 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     1381 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     1441 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     1501 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     1561 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gcaattcacc
     1621 cgcgcgcggg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     1681 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     1741 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     1801 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     1861 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     1921 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     1981 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcttcga
     2041 aggaagcgga gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     2101 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     2161 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     2221 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     2281 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     2341 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     2401 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     2461 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     2521 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     2581 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     2641 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     2701 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     2761 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     2821 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     2881 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2941 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3001 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3061 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3121 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3181 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3241 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3301 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3361 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3421 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     3481 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3541 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3601 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3661 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3721 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     3781 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3841 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3901 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3961 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4021 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4081 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     4141 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4201 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4261 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4321 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4381 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4441 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4501 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4561 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4621 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4681 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4741 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4801 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4861 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4921 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4981 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5041 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5101 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5161 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5221 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5281 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5341 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5401 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5461 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5521 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5581 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5641 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5701 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5761 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5821 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5881 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5941 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6001 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6061 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     6181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     6241 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     6301 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     6361 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     6421 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     6481 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     6541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6661 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     6721 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     6781 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     6841 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6901 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6961 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     7021 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     7081 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     7141 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     7201 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     7261 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     7321 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     7381 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     7441 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     7501 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     7561 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     7621 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     7681 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     7741 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     7801 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     7861 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7921 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     7981 ccccgagggg ac
//
