LOCUS       sequence-421060-        8800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses flag-tagged cFLIP (CFLAR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Nam M, Xia W, Mir AH, Jerrett A, Spinelli JB, Huang TT, Possemato R
  TITLE     Glucose limitation protects cancer cells from apoptosis induced by 
            pyrimidine restriction and replication inhibition.
  JOURNAL   Nat Metab. 2024 Dec;6(12):2338-2353. doi: 
            10.1038/s42255-024-01166-w. Epub 2024 Nov 26.
  PUBMED    39592843
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-024-01166-w"; journalName: "Nat Metab"; date: 
            "2024-12"; volume: "6"; issue: "12"; pages: "2338-2353"
COMMENT     Sequence Label: pLJM1 flag-cFLIP
FEATURES             Location/Qualifiers
     source          1..8800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1833..1853
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2142..2345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2296..2316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2386..2409
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3894..4011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4060..4570
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4489..4507
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4592..5191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4592..4611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5088..5108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5319..5552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5624..5745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5661..5680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5715..5734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5785..5920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5940..5959)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5941..5959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5969..5986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5969..5985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5978..6000)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6127..6582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6214..6233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6424..6445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6608..6712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6713..6781,6782..7573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6931..6950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7744..8332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8233..8252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8486..8503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8620..8641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8656..8661,8662..8679,8680..8686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8694..8710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8699..8721
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8718..8734
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8718..8734
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8753..8773
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8755..8773
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1981 ttttaaaatt atgttttaaa atggactatc atatgccaag tacgccccct attgacgtca
     2041 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2101 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2161 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2221 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2281 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2341 gagctggttt agtgaaccgt cagatccgct agcaccggta ccatggacta caaagacgat
     2401 gacgacaaga tgtctgctga agtcatccat caggttgaag aagcacttga tacagatgag
     2461 aaggagatgc tgctcttttt gtgccgggat gttgctatag atgtggttcc acctaatgtc
     2521 agggaccttc tggatatttt acgggaaaga ggtaagctgt ctgtcgggga cttggctgaa
     2581 ctgctctaca gagtgaggcg atttgacctg ctcaaacgta tcttgaagat ggacagaaaa
     2641 gctgtggaga cccacctgct caggaaccct caccttgttt cggactatag agtgctgatg
     2701 gcagagattg gtgaggattt ggataaatct gatgtgtcct cattaatttt cctcatgaag
     2761 gattacatgg gccgaggcaa gataagcaag gagaagagtt tcttggacct tgtggttgag
     2821 ttggagaaac taaatctggt tgccccagat caactggatt tattagaaaa atgcctaaag
     2881 aacatccaca gaatagacct gaagacaaaa atccagaagt acaagcagtc tgttcaagga
     2941 gcagggacaa gttacaggaa tgttctccaa gcagcaatcc aaaagagtct caaggatcct
     3001 tcaaataact tcaggctcca taatgggaga agtaaagaac aaagacttaa ggaacagctt
     3061 ggcgctcaac aagaaccagt gaagaaatcc attcaggaat cagaagcttt tttgcctcag
     3121 agcatacctg aagagagata caagatgaag agcaagcccc taggaatctg cctgataatc
     3181 gattgcattg gcaatgagac agagcttctt cgagacacct tcacttccct gggctatgaa
     3241 gtccagaaat tcttgcatct cagtatgcat ggtatatccc agattcttgg ccaatttgcc
     3301 tgtatgcccg agcaccgaga ctacgacagc tttgtgtgtg tcctggtgag ccgaggaggc
     3361 tcccagagtg tgtatggtgt ggatcagact cactcagggc tccccctgca tcacatcagg
     3421 aggatgttca tgggagattc atgcccttat ctagcaggga agccaaagat gttttttatt
     3481 cagaactatg tggtgtcaga gggccagctg gaggacagca gcctcttgga ggtggatggg
     3541 ccagcgatga agaatgtgga attcaaggct cagaagcgag ggctgtgcac agttcaccga
     3601 gaagctgact tcttctggag cctgtgtact gcggacatgt ccctgctgga gcagtctcac
     3661 agctcaccat ccctgtacct gcagtgcctc tcccagaaac tgagacaaga aagaaaacgc
     3721 ccactcctgg atcttcacat tgaactcaat ggctacatgt atgattggaa cagcagagtt
     3781 tctgccaagg agaaatatta tgtctggctg cagcacactc tgagaaagaa acttatcctc
     3841 tcctacacat aacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     3901 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     3961 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     4021 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     4081 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     4141 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     4201 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     4261 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     4321 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     4381 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     4441 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     4501 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     4561 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     4621 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4681 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4801 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4981 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5161 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     5221 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     5281 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5341 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5401 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5461 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5521 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5761 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5881 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5941 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6001 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6061 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6121 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6181 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6241 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6301 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6361 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8641 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8701 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     8761 ccctcactaa agggaacaaa agctggagct gcaagcttaa
//
