LOCUS       sequence-421063-       13559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with GmR and LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW14K_7G5
FEATURES             Location/Qualifiers
     source          1..13559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1689..4763
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(1709..1726)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1709..1725)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1718..1740)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(4935..4940,4941..4957,4958..4963)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5152..5685
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    7860..8152
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8800..9253
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(9281..9303)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9389..9529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9444..9463
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9544..9561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9715..10303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9795..9814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10482..11276)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10593..10612)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11203..11222
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        11715..11930
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11931..12734
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg
     1741 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1801 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1861 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
     1921 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
     1981 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
     2041 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
     2101 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
     2161 gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
     2221 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc
     2281 tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata
     2341 aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc
     2401 gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa
     2461 cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg
     2521 aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa
     2581 acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca
     2641 ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga
     2701 ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc
     2761 acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc
     2821 tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc
     2881 tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa
     2941 cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga
     3001 gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt
     3061 cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat
     3121 ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg
     3181 atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga
     3241 aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg
     3301 aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc
     3361 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
     3421 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
     3481 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc
     3541 tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg
     3601 aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg
     3661 cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta
     3721 aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca
     3781 cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct
     3841 ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca
     3901 tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
     3961 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
     4021 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
     4081 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
     4141 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta
     4201 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
     4261 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
     4321 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
     4381 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
     4441 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
     4501 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     4561 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     4621 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     4681 actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc
     4741 agttggtctg gtgtcaaaaa taacgcgcgc gtaactataa cggtcctaag gtagcgagct
     4801 tacttgtgct gttgtgtaag gacacgctta acccagagaa ttccagcgac ctattctacg
     4861 actacgcgtc catggctcga ggagctcggt accgccccac tacccgattt atcaagattt
     4921 ggcggagagt cccattgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta
     4981 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     5041 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc
     5101 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     5161 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt
     5221 atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct
     5281 cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac
     5341 tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac
     5401 caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt
     5461 agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc
     5521 accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac
     5581 gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg
     5641 gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaagtcga ctggcaaaca
     5701 gctattatgg gtattatggg tgtgtatcca gaacgacgga tcttgacgca atttggcgtg
     5761 gctctcttaa ggtagccctc cgaccaattg aggtgcgctc tgaccgatca gtttagactt
     5821 gtgtataaga gtcagagcgt cctgaacgga acctttcccg ttttccagga tctgatcttc
     5881 catgtgacct cctaacatgg taacgttcat gataacttct gctcttcatc gtgcggccga
     5941 ctgggctaaa tctgtgttct cttcggcggc gctgggtgat cctcgccgta ctgcccgctt
     6001 ggttaacgtc gccgcccaat tggcaaaata ttctggtaaa tcaataacca tctcatcaga
     6061 gggtagtgaa gccatgcagg aaggcgctta ccgatttatc cgcaatccca acgtttctgc
     6121 cgaggcgatc agaaaggctg gcgccatgca aacagtcaag ttggctcagg agtttcccga
     6181 actgctggcc attgaggaca ccacctcttt gagttatcgc caccaggtcg ccgaagagct
     6241 tggcaagctg ggctctattc aggataaatc ccgcggatgg tgggttcact ccgttctctt
     6301 gctcgaggcc accacattcc gcaccgtagg attactgcat caggagtggt ggatgcgccc
     6361 ggatgaccct gccgatgcgg atgaaaagga gagtggcaaa tggctggcag cggccgcaac
     6421 tagccggtta cgcatgggca gcatgatgag caacgtgatt gcggtctgtg accgcgaagc
     6481 cgatattcat gcttatctgc aggacaaact ggcgcataac gagcgcttcg tggtgcgctc
     6541 caagcaccca cgcaaggacg tagagtctgg gttgtatctg tacgaccatc tgaagaacca
     6601 accggagttg ggtggctatc agatcagcat tccgcaaaag ggcgtggtgg ataaacgcgg
     6661 taaacgtaaa aatcgaccag cccgcaaggc gagcttgagc ctgcgcagtg ggcgcatcac
     6721 gctaaaacag gggaatatca cgctcaacgc ggtgctggcc gaggagatta acccgcccaa
     6781 gggtgagacc ccgttgaaat ggttgttgct gaccagcgaa ccggtcgagt cgctagccca
     6841 agccttgcgc gtcatcgaca tttataccca tcgctggcgg atcgaggagt tccataaggc
     6901 atggaaaacc ggagcaggag ccgagaggca acgcatggag gagccggata atctggagcg
     6961 gatggtctcg atcctctcgt ttgttgcggt caggctgtta cagctcagag aaagcttcac
     7021 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     7081 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     7141 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     7201 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggga
     7261 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     7321 gcaggggatc aagatctgac ccgggactag ttggtgtgga agtcctaata tcggtgatca
     7381 atatagtggt tgacatgctg gctagtcaac attgagcctt ttgatcatgc aaatatatta
     7441 cggtatttta caatcaaata tcaaacttaa ctattgactt tataacttat ttaggtggta
     7501 acattcttat aaaaaagaaa aaaattactg caaaacagta ctagctttta acttgtatcc
     7561 taggttatct atgctgtctc accatagaga atattaccta tttcagaatg tatgtccatg
     7621 attcgccggg taaatacata taatacacaa atctggctta ataaagtcta taatatatct
     7681 cataaagaag tgctaaattg gctagtgcta tatattttta agaaaatttc ttttgactaa
     7741 gtccatatcg actttgtaaa agttcacatt agcatacata tattacacga gccagaaata
     7801 gtaacttttg cctaaatcac aaattgcaaa atttaattgc ttgcaaaagg tcacatgctt
     7861 ataatcaact tttttaaaaa tttaaaatac ttttttattt tttattttta aacataaatg
     7921 aaataattta tttattgttt atgattaccg aaacataaaa cctgctcaag aaaaagaaac
     7981 tgttttgtcc ttggaaaaaa agcactacct aggagcggcc aaaatgccga ggctttcata
     8041 gcttaaactc tttacagaaa ataggcatta tagatcagtt cgagttttct tattcttcct
     8101 tccggtttta tcgtcacagt tttacagtaa ataagtatca cctcttagag ttaacctata
     8161 ctaaaattta tgccagttaa ctatgagata agcaagtatc atctcatttc attacctgaa
     8221 gtcgagtaaa cagaaaatcc aattgttgat gaacctcaat gacttagaac tatctatcgg
     8281 cagatcatat aaagaggatt taggtactcg agaacgctcc gtacatgcag ctaaacctcc
     8341 aagcatctct aacttgggta gtgctttctc caccattttc tgtgcttgct ccttcgtggc
     8401 aagtccagcc cataatgccc agaatgtagt tgcggattcg tatgacgttc tgtgcttgat
     8461 ttttgtgttg tagtcaaaga aaaggtccgg ttaaacggat ctcgcattga tgaggcaacg
     8521 ctaattatca acatatagat tgttatctat ctgcatgaac acgaaatctt tacttgacga
     8581 cttgaggctg atggtgttta tgcaaagaaa ccactgtgtt taatatgtgt cactgtttga
     8641 tattactgtc agcgtagaag ataatagtaa aagcggttaa taagtgtatt tgagataagt
     8701 gtgataaagt ttttacagcg aaaagacgat aaatacaaga aaatgattac gaggatacgg
     8761 agagaggtat gtacatgtgt atttatatac taagctgccg gcggttgttt gcaagaccga
     8821 gaaaaggcta gcaagaatcg ggtcattgta gcgtatgcgc ctgtgaacat tctcttcaac
     8881 aagtttgatt ccattgcggt gaaatggtaa aagtcaaccc cctgcgatgt atattttcct
     8941 gtacaatcaa tcaaaaagcc aaatgattta gcattatctt tacatcttgt tattttacag
     9001 attttatgtt tagatctttt atgcttgctt ttcaaaaggc ctgcaggcaa gtgcacaaac
     9061 aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg acgaaatttg
     9121 ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca tcaacaccaa
     9181 taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca ccagctaaca
     9241 taaaatgttg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9301 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9361 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     9421 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     9481 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     9541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10141 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10441 ctaaagtata tatgagtaaa cttggtctga caggagctcg ctcagaagaa ctcgtcaaga
    10501 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
    10561 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
    10621 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
    10681 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
    10741 ggcatccgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
    10801 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
    10861 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
    10921 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
    10981 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
    11041 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttggagt
    11101 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
    11161 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
    11221 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
    11281 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc
    11341 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
    11401 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
    11461 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
    11521 cagtagctga cattcatccg ggcgatcgat ggaagccggc ggcacctcgc taacggattc
    11581 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
    11641 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatcggg
    11701 gggggggggg ggntttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
    11761 cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
    11821 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
    11881 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
    11941 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
    12001 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
    12061 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
    12121 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
    12181 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
    12241 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
    12301 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
    12361 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
    12421 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
    12481 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
    12541 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
    12601 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
    12661 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
    12721 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
    12781 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata
    12841 atgacgaaaa aaaaaaaatt ggaaagaaaa cccccccccc gcagcgttgg gtcctggcca
    12901 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
    12961 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
    13021 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
    13081 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
    13141 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
    13201 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
    13261 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
    13321 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
    13381 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
    13441 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
    13501 gatgagcttt accgctgggc cattctcatg aagaatatct tgaatttatt gtcatatta
//
