LOCUS       sequence-421065-       16186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn7 with GmR and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 16186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTSK56K_3G7
FEATURES             Location/Qualifiers
     source          1..16186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1204..1369
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             1790..2500
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1935..1953)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2203..2222
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        join(2672..2677,2678..2694,2695..2700)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2889..3422
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  9573..9797
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     misc_feature    10486..10778
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      11426..11879
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(11907..11929)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12015..12155
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12070..12089
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12170..12187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12341..12929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12421..12440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13108..13902)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13219..13238)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13829..13848
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        14340..14555
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             14556..15359
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaatgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     1261 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     1321 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttt atctgcagca
     1381 ctcaacagat ctccgtctac ttgcgacacc tctcttaagg tagctggacc ggattcgggt
     1441 ctagcctata tcgtcaatcc gttgatctat gtcgggtgcg gagaaagagg taatgaaatg
     1501 ggcgatcgcc cacagcttac acagacctaa tcgtagagtt cctagtaata cttagtggct
     1561 cgttacctcg ttcttccagg ccagggggcc gtacgcgctc ggcgaacagt ataagcagat
     1621 tcgactcact agggataaca gggtaatctc tgggtatcga ctcaatctcg cgctattgtg
     1681 ctatatccgt ggacggttgc ggtcaattct gtcggaaaat caacggatac gaacaattac
     1741 tcgcgacttc ctcaatacgt gctgcaaaac tttagaagga gatataccta tggtgagcaa
     1801 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     1861 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1921 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     1981 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     2041 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2101 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat
     2161 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     2221 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     2281 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     2341 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     2401 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     2461 ccgccactcc accggcggca tggacgagct gtacaagtaa cgcgcgcgta actataacgg
     2521 tcctaaggta gcgagcttac ttgtgctgtt gtgtaaggac acgcttaacc cagagaattc
     2581 cagcgaccta ttctacgact acgcgtccat ggctcgagga gctcggtacc gccccactac
     2641 ccgatttatc aagatttggc ggagagtccc attgacataa gcctgttcgg ttcgtaaact
     2701 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2761 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
     2821 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     2881 cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2941 agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca
     3001 aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact
     3061 cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg
     3121 cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca
     3181 agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc
     3241 ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg
     3301 gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata
     3361 caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct
     3421 aagtcgactg gcaaacagct attatgggta ttatgggtgt gtatccagaa cgacggatct
     3481 tgacgcaatt tggcgtggct ctcttaaggt agccctccga ccaattgagg tgcgctctga
     3541 ccgatcagtt tagtcactct tccccataaa ccatttccaa gaatctttgt gcttcctcag
     3601 ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta agctcaacat
     3661 cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa tcttccacac
     3721 tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga ttttttgcaa
     3781 gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt gggtgatcaa
     3841 ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt cgatctcttt
     3901 ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt tgattccagt
     3961 gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca ccctctaaag
     4021 actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag tctttatgct
     4081 taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt gtcgttatag
     4141 agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt tttggcaata
     4201 aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc ttacggaata
     4261 tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt ttctctagtg
     4321 cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga atgtgcttgc
     4381 ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac tgctcaaggc
     4441 ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa gcagctagtg
     4501 ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta tgacccaaag
     4561 cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa gcaccgtgtt
     4621 ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct tccccatacc
     4681 tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg ctcttaactc
     4741 tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac tccattaacc
     4801 gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg aataaggtat
     4861 gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt cttgctatca
     4921 cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca tacacctcat
     4981 ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca atagtgctat
     5041 aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg cttgtttaaa
     5101 taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca tggttttttc
     5161 aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat cccagtcgct
     5221 gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt ctagttctga
     5281 cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact tttgtcttgt
     5341 catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc ttgaatcgta
     5401 atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact cttgaagggc
     5461 tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt ggattaaccg
     5521 tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg ggatacccga
     5581 gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca cttgccgcaa
     5641 taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag cgagtacaaa
     5701 aagttttact acaatgtcca tcactccttg gcttagctca taccacacat cacggacctc
     5761 atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa
     5821 agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat cccagaatat
     5881 agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc
     5941 tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg tcacaaaaaa
     6001 gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa tttcatcaat
     6061 aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca acatggtttc
     6121 tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg ctcgatccaa
     6181 cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc agtctatttt
     6241 caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag gatacgtggc
     6301 tagaatacga tgaagagagg tcgtcttccc actaccagaa caaccaatta acaataagct
     6361 ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg tttgaacacg
     6421 ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc tgcctacgta
     6481 gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac ctaatggctg
     6541 aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg acttttgcaa
     6601 gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca ctgactcttg
     6661 taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc ccgtatcacg
     6721 ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg atcctgaaat
     6781 aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc cactgcgttg
     6841 aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc cgcacgtttt
     6901 ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat gcgtgttgat
     6961 ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg ctgaataaac
     7021 gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt attgtgtttt
     7081 tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg ccgactacgt
     7141 tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa ataaatcgta
     7201 tcaaccagca ctgggtcata agccgcttct aaatgttgag gtctagctat atcagtgctc
     7261 cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc ccacaaatta
     7321 acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg caactgctct
     7381 tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca tagctggaca
     7441 ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc catcaccaga
     7501 tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga
     7561 gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc ctctacaatg
     7621 ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc cactatgcct
     7681 ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact agaaactaag
     7741 gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc tggcaaacct
     7801 acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat ggccgttttg
     7861 tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt ttcaaagccg
     7921 atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag cgttggtctt
     7981 cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat cgtggcatca
     8041 atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact taagggtcgt
     8101 acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc tttagggtat
     8161 tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg gggaatgcga
     8221 ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt ctttgtacct
     8281 ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt
     8341 accttggttc cttcaccctt accatattct ctggctcggc caatctttgc tgttcctgtc
     8401 gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa tgcattaggc
     8461 gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt agtaacctta
     8521 tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa acgatcctta
     8581 ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa gctgacagaa
     8641 tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct aactaagcgg
     8701 ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac tcctttatca
     8761 gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg atacggatca
     8821 ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca tattcactac
     8881 ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa tattgaactt
     8941 aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta actccaaatc
     9001 ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt tttcttgcag
     9061 gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt ctgttttcac
     9121 tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat cagtaaaaat
     9181 gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt tttctaaggt
     9241 acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct caaaaggacc
     9301 atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac gaataacagg
     9361 atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata aggggaactg
     9421 ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag caagctctaa
     9481 gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt gggaacgacc
     9541 tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat gcccttggcc
     9601 acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc
     9661 cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact
     9721 attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac
     9781 tttattgtcc gcccacaccc gcttacgcag ggcatccatt tattactcaa ccgtaaccga
     9841 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
     9901 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
     9961 ctcgacccgg gactagttgg tgtggaagtc ctaatatcgg tgatcaatat agtggttgac
    10021 atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat
    10081 caaatatcaa acttaactat tgactttata acttatttag gtggtaacat tcttataaaa
    10141 aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc
    10201 tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa
    10261 tacatataat acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct
    10321 aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt
    10381 tgtaaaagtt cacattagca tacatatatt acacgagcca gaaatagtaa cttttgccta
    10441 aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac atgcttataa tcaacttttt
    10501 taaaaattta aaatactttt ttatttttta tttttaaaca taaatgaaat aatttattta
    10561 ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt ttgtccttgg
    10621 aaaaaaagca ctacctagga gcggccaaaa tgccgaggct ttcatagctt aaactcttta
    10681 cagaaaatag gcattataga tcagttcgag ttttcttatt cttccttccg gttttatcgt
    10741 cacagtttta cagtaaataa gtatcacctc ttagagttaa cctatactaa aatttatgcc
    10801 agttaactat gagataagca agtatcatct catttcatta cctgaagtcg agtaaacaga
    10861 aaatccaatt gttgatgaac ctcaatgact tagaactatc tatcggcaga tcatataaag
    10921 aggatttagg tactcgagaa cgctccgtac atgcagctaa acctccaagc atctctaact
    10981 tgggtagtgc tttctccacc attttctgtg cttgctcctt cgtggcaagt ccagcccata
    11041 atgcccagaa tgtagttgcg gattcgtatg acgttctgtg cttgattttt gtgttgtagt
    11101 caaagaaaag gtccggttaa acggatctcg cattgatgag gcaacgctaa ttatcaacat
    11161 atagattgtt atctatctgc atgaacacga aatctttact tgacgacttg aggctgatgg
    11221 tgtttatgca aagaaaccac tgtgtttaat atgtgtcact gtttgatatt actgtcagcg
    11281 tagaagataa tagtaaaagc ggttaataag tgtatttgag ataagtgtga taaagttttt
    11341 acagcgaaaa gacgataaat acaagaaaat gattacgagg atacggagag aggtatgtac
    11401 atgtgtattt atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa aggctagcaa
    11461 gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat
    11521 tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa
    11581 aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga
    11641 tcttttatgc ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa
    11701 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
    11761 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
    11821 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgttgcctc
    11881 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11941 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    12001 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    12061 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    12121 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    12181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    12241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12781 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    13021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    13081 agtaaacttg gtctgacagg agctcgctca gaagaactcg tcaagaaggc gatagaaggc
    13141 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
    13201 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
    13261 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
    13321 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt
    13381 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
    13441 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
    13501 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
    13561 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
    13621 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
    13681 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc
    13741 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
    13801 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
    13861 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
    13921 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
    13981 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg
    14041 ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc
    14101 tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
    14161 catccgggcg atcgatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
    14221 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
    14281 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atcggggggg ggggggggtt
    14341 tcaattcatc attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg
    14401 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
    14461 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
    14521 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
    14581 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
    14641 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
    14701 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
    14761 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
    14821 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
    14881 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
    14941 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
    15001 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
    15061 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
    15121 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
    15181 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
    15241 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
    15301 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
    15361 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
    15421 tatatcagtt attacccggg aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa
    15481 aaattggaaa gaaaaccccc cccccccgca gcgttgggtc ctggccacgg gtgcgcatga
    15541 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
    15601 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
    15661 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
    15721 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
    15781 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
    15841 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
    15901 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
    15961 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
    16021 acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
    16081 acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc
    16141 gctgggccat tctcatgaag aatatcttga atttattgtc atatta
//
