LOCUS       sequence-42108-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: NpuDnaEc-cGCaMP2
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(175..196)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(436..455)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             571..1014
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atgatcaaaa tagccacacg taaatattta ggcaaacaaa atgtctatga cattggagtt
       61 gagcgcgacc ataattttgc actcaaaaat ggcttcatag cttctaattg tttcaatggt
      121 accgctagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      181 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat
      241 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      301 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      361 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc
      421 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      481 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      541 ctggggcaca agctggagta caacacgcgt gaccaactga ctgaagagca gatcgcagaa
      601 tttaaagagg ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag
      661 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      721 atcaatgaag tagatgccga cggtaatggc acaatcgact tccctgagtt cctgacaatg
      781 atggcaagaa aaatgaaaga cacagacagt gaagaagaaa ttagagaagc gttccgtgtg
      841 tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac
      901 cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat
      961 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtga
//
