LOCUS       sequence-421136-        4729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 promoter 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_Promoter_5
FEATURES             Location/Qualifiers
     source          1..4729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(282..870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(362..381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1070..1861,1862..1930))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2035..2083
                     /note="synthetic polyadenylation signal"
     misc_feature    2097..2188
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2137..2156
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2711..2742
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2769..2778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2775..4427
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4471..4592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4482..4501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4536..4555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
       61 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
      121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1021 gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt taccagtgct
     1081 tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata gtggcctgac
     1141 tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc agcgcagcaa
     1201 tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac cagccagcag
     1261 ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag tctatgagct
     1321 gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga gttgtggcca
     1381 ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc aactctggtt
     1441 cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca gtcagctcct
     1501 tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc atggtaatgg
     1561 cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc gtgaccggcg
     1621 agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc tcttgcccgg
     1681 cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc atcatcggga
     1741 atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc agttcgatat
     1801 agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt
     1861 gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt
     1921 ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg
     1981 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
     2041 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     2101 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     2161 gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtaccctg ccgagagagg
     2221 ccagcggctg gctgaggctg tagagcagcg cgcggccggg ggcggcgggg gcagccagcg
     2281 cgggaggact cagctgcacc atgcggccgt cgccgggcag ctccgctgta accgaggcgg
     2341 gcgcgggggc cggggcgggc ccgggaggtc tgcacagctc ggtggtgggc acccgatggc
     2401 gcgcttcggc cgtcgccgcg tcgcggccct ttaagtctct cgcggcgccg cccccaccgg
     2461 cagggccgcc ccccgggcct ccgcgcgcgc ccagttcgat gactgggggc tccgctgcgg
     2521 ccgcgcggct cgtctccttg gcgacgccgt tcagcaggac caggtgcggg ggggccatgc
     2581 tggcctcggc cgcgtcccgt ccctctagct gggggtcact gcgagccgcc tcgctcggcg
     2641 gccgctcggt catcgagctc gctagcctcg aggatatcaa gatctggcct cggcggccaa
     2701 gcttagacac tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg
     2761 ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     2821 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
     2881 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     2941 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     3001 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     3061 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     3121 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     3181 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     3241 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     3301 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     3361 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     3421 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     3481 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     3541 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     3601 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     3661 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     3721 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     3781 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     3841 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     3901 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     3961 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     4021 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     4081 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     4141 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     4201 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     4261 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     4321 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     4381 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaataa ttctagagtc
     4441 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     4501 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4561 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4621 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4681 gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaacc
//
