LOCUS       sequence-421137-       15491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ
FEATURES             Location/Qualifiers
     source          1..15491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..246
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             247..427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    474..599
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1092..1325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1510..1554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1821..1937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1980..2191
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2204..2472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             10321..10344
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10360..10386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10360..10386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    10397..10969
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10564..10581)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10791..10810
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10978..11577
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10978..10997)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11474..11494
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11584..12172
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11637..11657)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12055..12066)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12246..12479
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12551..12672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12588..12607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12642..12661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12712..12847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12880..12896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12880..12896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12893..12915)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12904..12920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12928..12934,12935..12952,12953..12958))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12973..12994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13111..13128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13282..13870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14041..14832,14833..14901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14664..14683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14902..15006)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15074..15092
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15130..15152)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15252..15271
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15465..15487
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(15479..15491,1..5)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(15480..15491,1..5)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccagtgccaa gctgacgcgt gtagtcttat gcaatactct tgtagtcttg caacatggta
       61 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      121 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      181 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      241 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      301 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      361 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      421 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      481 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      541 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      601 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      661 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
      721 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
      781 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
      841 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
      901 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cactgatctt cagacctgga
      961 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1021 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1081 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1141 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1201 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1261 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1321 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1381 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1441 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1501 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1561 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1621 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1681 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     1741 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     1801 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     1861 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     1921 acaaaattca aaattttatc gatactagtg gatctgcgat cgctccggtg cccgtcagtg
     1981 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2041 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2101 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2161 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     2221 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     2281 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     2341 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     2401 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     2461 ccgttacaga tccaagctgt gaccggcgcc tactctagag ctagcgaatt cgaatttaaa
     2521 tcggatccta gcaccatgaa tcttctgcgt cggagtggga aacggcggcg ttcagaatca
     2581 ggctcagatt cgttctcggg aagcggcggt gacagcagtg ccagccccca gttcctctcc
     2641 gggtccgtgc tgagcccgcc gcccggcctt ggtcgctgcc tgaaggccgc agctgcagga
     2701 gaatgcaagc ctacagttcc tgactacgaa atagacaagc tactattggc aaactgggga
     2761 cttcctaaag cagttctgga aaaataccac agttttggtg taaaaaagat gtttgaatgg
     2821 caggcagagt gccttttgct tggacaagtc ctggaaggaa agaatttagt ttattcagct
     2881 cctacaagtg ctgggaagac tcttgtggca gaattactta ttttgaagcg ggttttggaa
     2941 atgcggaaga aagctttgtt tattcttccc tttgtttctg tggctaaaga gaagaaatac
     3001 tacctccaga gtctgtttca ggaagtagga ataaaagtag acggttatat gggcagcacc
     3061 tctccatcaa ggcatttctc ttcattggat attgcagtct gcacaattga gagagccaat
     3121 ggtctgatca atcgcctcat agaggaaaat aagatggatc tgttaggaat ggtggttgtg
     3181 gatgaattac atatgctggg agactctcac cgagggtatc tgctggaact tttgctgacc
     3241 aagatttgct atattactcg gaaatcagca tcttgtcagg cagatctagc cagttctctg
     3301 tctaatgctg tgcaaatcgt tggcatgagt gctacccttc ctaatttgga gcttgtggct
     3361 tcctggttga atgctgaact ctaccatacc gactttcgcc ctgtaccgct tttggagtca
     3421 gtaaaagttg gaaattccat atatgactct tcaatgaaac ttgtgaggga atttgagccc
     3481 atgcttcaag tgaagggaga tgaggaccat gttgttagct tatgttatga gacgatttgt
     3541 gataaccatt cagtattact tttttgtcca tcaaagaaat ggtgtgagaa gctggcagat
     3601 atcattgctc gagagtttta taatctacat catcaagctg agggattggt gaaaccctct
     3661 gaatgcccac cagtaattct ggaacaaaaa gaactcctgg aagtgatgga tcagttaaga
     3721 cggttgcctt caggactgga ctctgtatta cagaaaactg taccatgggg agtagcattt
     3781 catcatgcag gtcttacttt tgaggagagg gatatcattg aaggagcctt tcgtcaaggt
     3841 ctcattcgag tcttggcggc aacttctact ctttcttctg gggtgaattt acctgcacgt
     3901 cgtgtgatta ttcgaacccc tatttttggt ggtcgacctc tagatattct tacttataag
     3961 cagatggttg gtcgtgctgg caggaaagga gtggacacag taggcgagag tatcttaatt
     4021 tgtaagaact ctgagaaatc aaaaggcata gctctccttc agggttctct aaagcctgtt
     4081 cgcagctgtc tgcaaagacg agaaggagaa gaagtaactg gcagcatgat acgagctatt
     4141 ctggagataa tagttggtgg agtggcaagt acatcacaag atatgcatac ttatgctgcc
     4201 tgcacatttt tggctgcaag tatgaaagaa gggaagcaag gaattcagag aaatcaagag
     4261 tctgttcagc ttggagcgat tgaggcctgt gtgatgtggc tactagaaaa tgaattcatc
     4321 cagagtacag aagccagtga tggaacagaa ggaaaggtgt atcatccaac acatcttggt
     4381 tcggccactc tttcttcttc actttctcca gctgatactt tagatatttt tgctgacctg
     4441 caaagagcaa tgaagggctt tgttttagag aatgatcttc atattctcta tctggttaca
     4501 cctatgtttg aggattggac tactattgat tggtatcgat ttttctgttt atgggagaag
     4561 ttgccaactt caatgaaaag ggtggcagag ctagtgggag ttgaagaggg gttcttggcc
     4621 cgttgtgtga aaggaaaagt agtagccaga actgagagac agcatcgaca aatggccatc
     4681 cataaaaggt ttttcaccag tcttgtgcta ttagatttaa tcagtgaagt tcccttaagg
     4741 gaaataaatc agaaatatgg atgcaatcgt gggcagattc aatctttgca acagtcagct
     4801 gctgtttatg cagggatgat tacagtattt tccaaccgtc tgggctggca caacatggaa
     4861 ctactacttt cccaatttca gaagcgtctt acgtttggca tccagaggga gctgtgtgac
     4921 ctggttcggg tatccttact aaatgctcag agagccaggg ttctctatgc ttctggcttt
     4981 catactgtgg cagaccttgc tagagcaaat attgtggagg tggaggtgat tctgaaaaat
     5041 gctgtgcctt tcaaaagtgc ccggaaggca gtggatgagg aagaggaagc agttgaagaa
     5101 cgtcgcaata tgcgaactat ctgggtgact ggcagaaaag gtttaactga aagggaagca
     5161 gcagccctta tagtggaaga agccagaatg attctgcagc aggacttagt tgaaatggga
     5221 gtgcaatgga atccatgtgc cctgttacat tctagtacat gctcattgac tcatagtgag
     5281 tccgaagtaa aggaacacac atttatatcc caaactaaga gttcttataa aaaattaaca
     5341 tcaaagaaca aaagtaacac aatatttagt gattcttata ttaagcattc accaaatata
     5401 gtgcaagact taaataaaag tagagagcat acaagttcct ttaattgtaa tttccagaat
     5461 gggaatcaag aacatcagag atgttccatt ttcagagcaa gaaaacgggc ctctttagat
     5521 ataaataaag agaagccagg agcctctcag aatgagggga aaacaagtga taagaaagtt
     5581 gttcagactt tttcacagaa aacaaaaaag gcacctttga atttcaattc agaaaagatg
     5641 agcagaagtt ttcgatcttg gaaacgtaga aagcatctaa agcgatctag ggacagcagc
     5701 cccctgaaag actctggagc gtgtagaatc catttacaag gacagactct gtctaatcct
     5761 agtctttgtg aagacccgtt taccttagat gagaagaaaa cggaatttag aaattcaggg
     5821 ccatttgcta aaaatgtatc tttgagtggt aaggaaaaag ataataaaac atcattccca
     5881 ttacaaataa agcaaaattg ttcatggaac ataacactaa ctaatgataa ttttgtggag
     5941 catattgtca caggatctca gagtaaaaat gtgacttgtc aggccactag tgtggttagt
     6001 gaaaagggca gaggagtagc tgttgaggca gaaaaaataa atgaagtgct gatacaaaat
     6061 ggttcaaaaa accagaatgt ttatatgaaa caccatgaca tccatccaat taaccagtac
     6121 ctgcgaaagc aatctcatga acagacaagc actattacca aacagaaaaa tataatagag
     6181 agacaaatgc cctgtgaagc agtcagtagt tacataaata gagactcaaa tgttactatc
     6241 aattgtgaaa ggataaagct taatacagag gaaaataaac caagtcattt tcaggcatta
     6301 ggagatgata taagcagaac tgtgataccc agtgaagtac ttccatcagc tggagcattt
     6361 agcaaatcag aaggccagca tgagaatttt ctaaatattt ctagactaca agaaaaaaca
     6421 ggtacttata caacaaacaa aactaaaaat aatcatgttt ctgacttagg tttagtcctc
     6481 tgtgattttg aagatagttt ctatctggat actcagtcag agaaaataat acaacagatg
     6541 gcaactgaaa atgccaaact aggagcaaag gacaccaacc tggcagcagg gataatgcag
     6601 aagagcttag tccaacagaa ctcaatgaac tcttttcaga aggagtgtca cattcctttt
     6661 cctgctgaac agcaccctct aggagcgact aagatagatc atttggacct taagactgta
     6721 ggtactatga aacaaagcag tgattcacat ggggttgata tcctgactcc agaaagcccg
     6781 attttccatt ctccaatact attggaggaa aatggtcttt ttttaaaaaa gaatgaagtt
     6841 tctgttactg attcacaatt aaatagtttt cttcaaggtt atcaaacaca agaaactgtg
     6901 aaaccagtta tacttctgat tcctcaaaag agaactccca ctggtgtaga aggagaatgt
     6961 cttccagttc ctgaaacaag tttgaatatg agtgatagtt tactatttga tagcttcagt
     7021 gatgactatc tagtaaaaga acaattacct gatatgcaaa tgaaagaacc ccttccttca
     7081 gaagtaacat caaaccattt tagtgattct ctgtgtctac aagaagacct aattaaaaaa
     7141 tcaaatgtaa atgagaatca agatacccac cagcagttga cttgttccaa tgatgaatct
     7201 attatatttt cagaaatgga ttctgttcag atggttgaag ctttggacaa tgtggatata
     7261 tttcctgtcc aagagaagaa tcatactgta gtatctccta gagcattaga actaagtgat
     7321 ccagtacttg atgagcacca ccaaggtgat caagatggag gagatcaaga tgaaagggct
     7381 gaaaaatcaa aattaactgg gaccaggcaa aatcattcat tcatttggtc aggggcatca
     7441 tttgatctaa gtccaggact gcaaaggatt ttagataaag tatccagtcc tctagaaaat
     7501 gaaaagctaa aatcaatgac tataaacttt tccagtttga atagaaaaaa tacagagtta
     7561 aatgaagaac aagaagttat ttcaaacttg gagacaaaac aagtgcaggg aatttcattt
     7621 tcttctaata atgaagtaaa aagcaagatt gagatgctag aaaacaatgc caatcatgat
     7681 gaaacctcat ccctcttacc tcgtaaagaa agtaatatag ttgatgataa tggtctcatt
     7741 cctcctacac ccattccaac atctgcttct aagctgacat ttccagggat tcttgaaaca
     7801 cctgtaaacc cttggaaaac taataatgtt ttacaacctg gtgaaagtta tttatttggc
     7861 tcaccttcag atattaaaaa ccacgattta agtccaggga gtagaaatgg gttcaaagac
     7921 aacagcccta ttagtgacac aagcttttca cttcagttat cacaggatgg attacagtta
     7981 actccagcct caagcagttc agaaagtttg tccataattg atgtagcaag tgaccaaaat
     8041 cttttccaaa cattcattaa ggagtggcgg tgcaaaaagc gattttccat ctcactggct
     8101 tgtgaaaaga ttagaagttt gacatcttct aaaactgcta ctattggcag taggtttaag
     8161 caagctagct cacctcagga aattcctatt agagatgatg gatttcccat taaaggttgt
     8221 gatgacacct tggtggttgg actggcagta tgctggggtg gaagggatgc ctattatttt
     8281 tcactgcaga aggaacaaaa gcattctgaa attagtgcca gtttggttcc accttcttta
     8341 gatccaagcc tgactttgaa agacaggatg tggtaccttc aatcttgctt gcgaaaggaa
     8401 tctgataaag aatgttctgt tgtcatctat gacttcatcc agagctataa aattcttctt
     8461 ctttcttgtg gcatctcctt ggagcaaagt tatgaagatc ctaaggtggc atgctggtta
     8521 ctagatccag attctcagga gccgactctt catagcatag ttaccagttt tcttcctcat
     8581 gagcttccac tcctagaagg gatggagacc agccaaggga ttcaaagcct ggggctaaat
     8641 gctggcagtg agcattctgg gcgatacaga gcatctgtgg agtccattct catcttcaac
     8701 tctatgaatc agctcaactc tttgttgcag aaggaaaacc ttcaagatgt tttccgtaag
     8761 gtggaaatgc cctctcagta ctgcttggcc ttgctagaac taaatggaat tggctttagt
     8821 actgcagaat gtgaaagtca gaaacatata atgcaagcca agctggatgc aattgagacc
     8881 caggcctatc aactagctgg ccacagtttt tctttcacca gttcagatga catcgctgag
     8941 gttttatttt tggaattgaa gttgccccca aatagagaga tgaaaaacca aggcagcaag
     9001 aaaactctgg gttctaccag aagagggatt gacaatggac gcaagctaag gctgggaaga
     9061 cagttcagca ctagtaagga cgttttaaat aaattaaagg cattacatcc tttaccaggc
     9121 ttgatattag aatggagaag aatcactaat gctattacca aagtggtctt tccccttcag
     9181 cgggaaaagt gtcttaatcc ttttcttgga atggaaagaa tctatcctgt atcacagtcg
     9241 cacactgcta caggacgaat aacctttaca gaaccaaata ttcagaatgt gccaagagat
     9301 tttgaaatca aaatgccaac actagtagga gaaagcccac cttctcaagc tgtaggcaaa
     9361 ggcctacttc ccatgggcag aggaaaatat aagaagggtt tcagcgtgaa tcctagatgc
     9421 caggcacaga tggaggagag agctgcagac agaggaatgc cattttcaat tagcatgcga
     9481 catgcctttg tgcctttccc aggtggttca atactggctg ctgactactc tcagcttgaa
     9541 ctgaggatct tggctcattt atcccatgat cgtcgtctca ttcaagtgtt aaacactgga
     9601 gctgatgttt tcaggagcat tgcagcagag tggaagatga ttgagccaga gtctgttggg
     9661 gatgatctga ggcagcaggc aaaacagatt tgctatggga tcatttatgg aatgggagct
     9721 aaatctttgg gagagcagat gggcattaaa gaaaatgatg ctgcatgcta tattgactcc
     9781 ttcaaatcca gatacacagg gattaatcaa ttcatgacag agacagtgaa gaattgtaaa
     9841 agagacggat ttgttcagac cattttggga aggcgtagat atttgccagg aatcaaagac
     9901 aacaaccctt atcgtaaagc tcacgctgag cgtcaagcta tcaacacaat agtccaagga
     9961 tcagcagctg atattgtcaa aatagccaca gttaacattc agaagcaatt agagaccttc
    10021 cactcaacct tcaaatccca tggtcatcga gagggtatgc tccaaagtga ccgaacagga
    10081 ttgtcacgaa agagaaaact gcaagggatg ttctgcccaa tcagaggagg cttcttcatc
    10141 cttcaactcc atgatgaact cctatatgaa gtggcagaag aagatgttgt tcaggtagct
    10201 cagattgtca agaatgaaat ggaaagtgct gtaaaactgt ctgtgaaatt gaaagtgaaa
    10261 gtgaaaatag gcgccagctg gggagagcta aaggactttg atgtggcggc cgctggagga
    10321 gactacaagg acgacgatga caagtcggcc gctggaggat acccctacga cgtgcccgac
    10381 tacgcctagg gccgcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
    10441 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
    10501 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
    10561 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
    10621 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
    10681 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
    10741 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
    10801 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
    10861 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
    10921 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac ctccggaatg
    10981 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
    11041 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
    11101 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
    11161 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
    11221 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
    11281 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
    11341 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
    11401 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
    11461 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
    11521 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaggtc
    11581 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    11641 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    11701 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    11761 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    11821 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    11881 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    11941 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    12001 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    12061 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    12121 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
    12181 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    12241 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
    12301 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    12361 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    12421 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    12481 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    12541 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    12601 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    12661 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
    12721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    12781 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    12841 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
    12901 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    12961 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    13021 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    13081 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    13141 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    13201 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    13261 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    13321 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    13381 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    13441 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    13501 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13561 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13621 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13681 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    13741 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    13801 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    13861 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    13921 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    13981 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    14041 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    14101 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    14161 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    14221 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    14281 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    14341 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    14401 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    14461 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    14521 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14581 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14641 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14701 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14761 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14821 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    14881 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    14941 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    15001 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    15061 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    15121 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    15181 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    15241 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    15301 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    15361 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    15421 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    15481 taaaacgacg g
//
