LOCUS       sequence-421140-       11428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY1052 PASTEv3
            pCMV-SpCas9-XTEN3-RT(L139P)-(G...
FEATURES             Location/Qualifiers
     source          1..11428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(155..174)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        346..725
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     880..900
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     971..990
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        971..989
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1039..1059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1060..3573,3574..3576,3577..5160)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1060..5160
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7429..7449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7504..7524
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7525..7551
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9077..9094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9083..9307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9378..9394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9378..9394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9391..9413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9402..9418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9426..9432,9433..9450,9451..9456))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9471..9492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9609..9626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9780..10368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9860..9879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10539..11330,11331..11399))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11162..11181
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11400..11428,1..76))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
       61 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
      121 atcgatctcc cgatccccta gggtctactc tcagtacaat ctgctctgat gccgcatagt
      181 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
      241 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
      301 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
      361 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      421 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      481 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      721 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      781 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      841 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      901 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat
      961 ccgcggccgc taatacgact cactataggg agagccgcca ccatgaaacg gacagccgac
     1021 ggaagcgagt tcgagtcacc aaagaagaag cggaaagtcg acaagaagta cagcatcggc
     1081 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1141 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1201 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1261 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1321 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1381 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1441 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1501 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1561 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1621 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1681 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1741 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1801 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1861 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1921 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1981 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     2041 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     2101 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2161 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2221 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2281 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     2341 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2401 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2461 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2521 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2581 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2641 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2701 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2761 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2821 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2881 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2941 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     3001 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     3061 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3121 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3181 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3241 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3301 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3361 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3421 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3481 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3541 gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag ctttctgaag
     3601 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3661 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3721 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3781 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3841 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3901 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3961 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     4021 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     4081 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4141 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4201 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4261 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4321 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     4381 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     4441 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     4501 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4561 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     4621 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4681 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     4741 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4801 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4861 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4921 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4981 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     5041 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     5101 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac
     5161 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca
     5221 acaccagaga gctctggtag cgagacaccc ggtaccagtg aaagcgccac gccagaaagc
     5281 agtgggagtg agactccggg tacatctgaa tcagcgacac cggaatcaag tggcggcagc
     5341 agtggcggca gcagcaccct aaatatagaa gatgagtatc ggctacatga gacctcaaaa
     5401 gagccagatg tttctctagg gtccacatgg ctgtctgatt ttcctcaggc ctgggcggaa
     5461 accgggggca tgggactggc agttcgccaa gctcctctga tcatacctct gaaagcaacc
     5521 tctacccccg tgtccataaa acaatacccc atgtcacaag aagccagact ggggatcaag
     5581 ccccacatac agagactgtt ggaccaggga atactggtac cctgccagtc cccctggaac
     5641 acgcccctgc tacccgttaa gaaaccaggg actaatgatt ataggcctgt ccaggatctg
     5701 agagaagtca acaagcgggt ggaagacatc caccccaccg tgcccaaccc ttacaacctc
     5761 ttgagcgggc ccccaccgtc ccaccagtgg tacactgtgc ttgatttaaa ggatgccttt
     5821 ttctgcctga gactccaccc caccagtcag cctctcttcg cctttgagtg gagagatcca
     5881 gagatgggaa tctcaggaca attgacctgg accagactcc cacagggttt caaaaacagt
     5941 cccaccctgt ttaatgaggc actgcacaga gacctagcag acttccggat ccagcaccca
     6001 gacttgatcc tgctacagta cgtggatgac ttactgctgg ccgccacttc tgagctagac
     6061 tgccaacaag gtactcgggc cctgttacaa accctaggga acctcgggta tcgggcctcg
     6121 gccaagaaag cccaaatttg ccagaaacag gtcaagtatc tggggtatct tctaaaagag
     6181 ggtcagagat ggctgactga ggccagaaaa gagactgtga tggggcagcc tactccgaag
     6241 acccctcgac aactaaggga gttcctaggg aaggcaggct tctgtcgcct cttcatccct
     6301 gggtttgcag aaatggcagc ccccctgtac cctctcacca aaccggggac tctgtttaat
     6361 tggggcccag accaacaaaa ggcctatcaa gaaatcaagc aagctcttct aactgcccca
     6421 gccctggggt tgccagattt gactaagccc tttgaactct ttgtcgacga gaagcagggc
     6481 tacgccaaag gtgtcctaac gcaaaaactg ggaccttggc gtcggccggt ggcctacctg
     6541 tccaaaaagc tagacccagt agcagctggg tggccccctt gcctacggat ggtagcagcc
     6601 attgccgtac tgacaaagga tgcaggcaag ctaaccatgg gacagccact agtcattctg
     6661 gccccccatg cagtagaggc actagtcaaa caaccccccg accgctggct ttccaacgcc
     6721 cggatgactc actatcaggc cttgcttttg gacacggacc gggtccagtt cggaccggtg
     6781 gtagccctga acccggctac gctgctccca ctgcctgagg aagggctgca acacaactgc
     6841 cttgatatcc tggccgaagc ccacggaacc cgacccgacc taacggacca gccgctccca
     6901 gacgccgacc acacctggta cacggatgga agcagtctct tacaagaggg acagcgtaag
     6961 gcgggagctg cggtgaccac cgagaccgag gtaatctggg ctaaagccct gccagccggg
     7021 acatccgctc agcgggctga actgatagca ctcacccagg ccctaaagat ggcagaaggt
     7081 aagaagctaa atgtttatac tgatagccgt tatgcttttg ctactgccca tatccatgga
     7141 gaaatataca gaaggcgtgg gtggctcaca tcagaaggca aagagatcaa aaataaagac
     7201 gagatcttgg ccctactaaa agccctcttt ctgcccaaaa gacttagcat aatccattgt
     7261 ccaggacatc aaaagggaca cagcgccgag gctagaggca accggatggc tgaccaagcg
     7321 gcccgaaagg cagccatcac agagactcca gacacctcta ccctcctcat agaaaattca
     7381 tcaccctctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc caagaagaag
     7441 aggaaagtcg gggggtcagg tggatccggc ggaagtggcg gatccggtgg atctggcggc
     7501 agtccaaaaa agaaaagaaa agtgtatccc tatgatgtcc ccgattatgc cggttcaaga
     7561 gccctggtcg tgattagact gagccgagtg acagacgcca ccacaagtcc cgagagacag
     7621 ctggaatcat gccagcagct ctgtgctcag cggggttggg atgtggtcgg cgtggcagag
     7681 gatctggacg tgagcggggc cgtcgatcca ttcgacagaa agaggaggcc caacctggca
     7741 agatggctcg ctttcgagga acagcccttt gatgtgatcg tcgcctacag agtggaccgg
     7801 ctgacccgct caattcgaca tctccagcag ctggtgcatt gggctgagga ccacaagaaa
     7861 ctggtggtca gcgcaacaga agcccacttc gatactacca caccttttgc cgctgtggtc
     7921 atcgcactga tgggcactgt ggcccagatg gagctcgaag ctatcaagga gcgaaacagg
     7981 agcgcagccc atttcaatat tagggccggt aaatacagag gctccctgcc cccttgggga
     8041 tatctcccta ccagggtgga tggggagtgg agactggtgc cagaccccgt ccagagagag
     8101 cggattctgg aagtgtacca cagagtggtc gataaccacg aaccactcca tctggtggca
     8161 cacgacctga atagacgcgg cgtgctctct ccaaaggatt attttgctca gctgcaggga
     8221 agagagccac agggaagaga atggagtgct actgcactga agagatctat gatcagtgag
     8281 gctatgctgg gttacgcaac actcaatggc aaaactgtcc gggacgatga cggagcccct
     8341 ctggtgaggg ctgagcctat tctcaccaga gagcagctcg aagctctgcg ggcagaactg
     8401 gtcaagacta gtcgcgccaa acctgccgtg agcaccccaa gcctgctcct gagggtgctg
     8461 ttctgcgccg tctgtggaga gccagcatac aagtttgccg gcggagggcg caaacatccc
     8521 cgctatcgat gcaggagcat ggggttccct aagcactgtg gaaacgggac agtggccatg
     8581 gctgagtggg acgccttttg cgaggaacag gtgctggatc tcctgggtga cgctgagcgg
     8641 ctggaaaaag tgtgggtggc aggatctgac tccgctgtgg agctggcaga agtcaatgcc
     8701 gagctcgtgg atctgacttc cctcatcgga tctcctgcat atagagctgg gtccccacag
     8761 agagaagctc tggacgcacg aattgctgca ctcgctgcta gacaggagga actggagggc
     8821 ctggaggcca ggccctctgg atgggagtgg cgagaaaccg gacagaggtt tggggattgg
     8881 tggagggagc aggacaccgc agccaagaac acatggctga gatccatgaa tgtccggctc
     8941 acattcgacg tgcgcggtgg cctgactcga accatcgatt ttggcgacct gcaggagtat
     9001 gaacagcacc tgagactggg gtccgtggtc gaaagactgc acactgggat gtcctaggtt
     9061 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9121 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9181 gagaaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9241 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9301 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     9361 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     9481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10381 atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg gattttggtc
    10441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11401 ctcttccttt ttcaatatta ttgaagca
//
